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The map label for this gene is prfB [H]

Identifier: 73540774

GI number: 73540774

Start: 1162179

End: 1163252

Strand: Reverse

Name: prfB [H]

Synonym: Reut_A1072

Alternate gene names: 73540774

Gene position: 1163252-1162179 (Counterclockwise)

Preceding gene: 73540776

Following gene: 73540773

Centisome position: 30.56

GC content: 61.73

Gene sequence:

>1074_bases
GTGCCATCTCCGATCTCCGTTCCCGGACGGAAGATCTACGGGGGTATCTTTGACTACGATGTCAAAGTAGGAAGGCTGGA
AGAAGTCAACGGTTTGCTGGAAGACCCGGACGTCTGGAACAACCCCAAGCGCGCCCAGGATCTCGGCAAGGAAAAGAAAG
CACTCGAGGGCGTGGTAAGCGTTCTCGGCAAGCTCACGGACGACCTCAACGGTGCCGATGAGCTGTTTGAACTCGCCAAG
GAAGAAGGCGACGACGAAACGCTCGAGTCGATCGAGACCGACGTCAAGGGCTTCGAGGAAATCGTCGCCGGCATGGAATT
CCGCCGCATGTTCGCGGGCGAGATGGACCAGGCCAACGCCTTTATCGACATCCAGGCCGGCGCCGGCGGCACCGAAGCGT
GCGACTGGGCGTCGATGCTGCTGCGCCAGTACCTGAAGTACTGCGAGCGCAAGGGCTTCAAGGCCGAGGTGCTCGAAGAG
TCCGAAGGCGACGTGGCCGGCATCAAGAGTGCGACGATCAAGATCGAAGGCGAATACGCGTTCGGCTACCTGCGCACCGA
AACCGGCGTGCACCGCCTCGTGCGCAAGTCGCCGTTCGATTCGTCGGGCGGGCGCCATACGTCGTTCTCGTCGGTGTTCG
TATACCCGGAAGTCGACGACTCGTTCGAGGTCGAGGTCAACCCGGCCGATCTGCGCGTCGATACCTACCGCGCTTCGGGC
GCCGGCGGCCAGCACATCAACAAGACCGATTCGGCCGTGCGTATCACGCACATCCCGACCGGCATCGTCGTGCAGTGTCA
GAACGACCGTTCGCAGCACCGCAACCGTGCCGAGGCCATGTCGATGCTGAAGTCGCGCCTGTACGAGCACGAGATGCGCA
AGCGCCAGGCCGAAGCCGACAAGCTCGAAGCCGGCAAGACCGATGTGGGCTGGGGCCACCAGATCCGTTCGTACGTGCTG
GATCAAAGCCGTATCAAGGACCTGCGTACCAACGTCGAAATGTCCAACACCCAGAAGGTGCTGGACGGCGACCTGGACCC
GTTCATCGAGGCCAGCCTCAAGCAGGGACTGTAA

Upstream 100 bases:

>100_bases
CGCCGCGCGGCGGTCCGAAATACGGCCCGATTCCGCTATAATTCAAGGTTTTCGAAGCGCAGGATCATCATGGAAGCAGA
ACGCCTCAACGCCATCTCCG

Downstream 100 bases:

>100_bases
GGCCGCTGCCGTGCCCGCCATGCGGGCATGGCCGACCGCAACCGCCAACGACTCACCGCCCGGCGCGGCGGCTCCTCCAA
CTGGGGCACCATGAGCGAAG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MPSPISVPGRKIYGGIFDYDVKVGRLEEVNGLLEDPDVWNNPKRAQDLGKEKKALEGVVSVLGKLTDDLNGADELFELAK
EEGDDETLESIETDVKGFEEIVAGMEFRRMFAGEMDQANAFIDIQAGAGGTEACDWASMLLRQYLKYCERKGFKAEVLEE
SEGDVAGIKSATIKIEGEYAFGYLRTETGVHRLVRKSPFDSSGGRHTSFSSVFVYPEVDDSFEVEVNPADLRVDTYRASG
AGGQHINKTDSAVRITHIPTGIVVQCQNDRSQHRNRAEAMSMLKSRLYEHEMRKRQAEADKLEAGKTDVGWGHQIRSYVL
DQSRIKDLRTNVEMSNTQKVLDGDLDPFIEASLKQGL

Sequences:

>Translated_357_residues
MPSPISVPGRKIYGGIFDYDVKVGRLEEVNGLLEDPDVWNNPKRAQDLGKEKKALEGVVSVLGKLTDDLNGADELFELAK
EEGDDETLESIETDVKGFEEIVAGMEFRRMFAGEMDQANAFIDIQAGAGGTEACDWASMLLRQYLKYCERKGFKAEVLEE
SEGDVAGIKSATIKIEGEYAFGYLRTETGVHRLVRKSPFDSSGGRHTSFSSVFVYPEVDDSFEVEVNPADLRVDTYRASG
AGGQHINKTDSAVRITHIPTGIVVQCQNDRSQHRNRAEAMSMLKSRLYEHEMRKRQAEADKLEAGKTDVGWGHQIRSYVL
DQSRIKDLRTNVEMSNTQKVLDGDLDPFIEASLKQGL
>Mature_356_residues
PSPISVPGRKIYGGIFDYDVKVGRLEEVNGLLEDPDVWNNPKRAQDLGKEKKALEGVVSVLGKLTDDLNGADELFELAKE
EGDDETLESIETDVKGFEEIVAGMEFRRMFAGEMDQANAFIDIQAGAGGTEACDWASMLLRQYLKYCERKGFKAEVLEES
EGDVAGIKSATIKIEGEYAFGYLRTETGVHRLVRKSPFDSSGGRHTSFSSVFVYPEVDDSFEVEVNPADLRVDTYRASGA
GGQHINKTDSAVRITHIPTGIVVQCQNDRSQHRNRAEAMSMLKSRLYEHEMRKRQAEADKLEAGKTDVGWGHQIRSYVLD
QSRIKDLRTNVEMSNTQKVLDGDLDPFIEASLKQGL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=248, Percent_Identity=38.7096774193548, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI34577120, Length=320, Percent_Identity=33.125, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI166795305, Length=147, Percent_Identity=40.1360544217687, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI2367172, Length=346, Percent_Identity=65.0289017341041, Blast_Score=475, Evalue=1e-135,
Organism=Escherichia coli, GI1787462, Length=244, Percent_Identity=44.2622950819672, Blast_Score=209, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17542784, Length=305, Percent_Identity=29.5081967213115, Blast_Score=111, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=242, Percent_Identity=37.1900826446281, Blast_Score=154, Evalue=3e-38,
Organism=Drosophila melanogaster, GI19921226, Length=209, Percent_Identity=42.5837320574163, Blast_Score=159, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39767; Mature: 39636

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPISVPGRKIYGGIFDYDVKVGRLEEVNGLLEDPDVWNNPKRAQDLGKEKKALEGVVS
CCCCCCCCCHHHCCCEEECCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VLGKLTDDLNGADELFELAKEEGDDETLESIETDVKGFEEIVAGMEFRRMFAGEMDQANA
HHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
FIDIQAGAGGTEACDWASMLLRQYLKYCERKGFKAEVLEESEGDVAGIKSATIKIEGEYA
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEECCEEEEEEEECCEE
FGYLRTETGVHRLVRKSPFDSSGGRHTSFSSVFVYPEVDDSFEVEVNPADLRVDTYRASG
EEEEEHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCEEEEEEECCC
AGGQHINKTDSAVRITHIPTGIVVQCQNDRSQHRNRAEAMSMLKSRLYEHEMRKRQAEAD
CCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLEAGKTDVGWGHQIRSYVLDQSRIKDLRTNVEMSNTQKVLDGDLDPFIEASLKQGL
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
PSPISVPGRKIYGGIFDYDVKVGRLEEVNGLLEDPDVWNNPKRAQDLGKEKKALEGVVS
CCCCCCCCHHHCCCEEECCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VLGKLTDDLNGADELFELAKEEGDDETLESIETDVKGFEEIVAGMEFRRMFAGEMDQANA
HHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
FIDIQAGAGGTEACDWASMLLRQYLKYCERKGFKAEVLEESEGDVAGIKSATIKIEGEYA
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEECCEEEEEEEECCEE
FGYLRTETGVHRLVRKSPFDSSGGRHTSFSSVFVYPEVDDSFEVEVNPADLRVDTYRASG
EEEEEHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCEEEEEEECCC
AGGQHINKTDSAVRITHIPTGIVVQCQNDRSQHRNRAEAMSMLKSRLYEHEMRKRQAEAD
CCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLEAGKTDVGWGHQIRSYVLDQSRIKDLRTNVEMSNTQKVLDGDLDPFIEASLKQGL
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA