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The map label for this gene is lysS [H]

Identifier: 73540772

GI number: 73540772

Start: 1159731

End: 1161281

Strand: Reverse

Name: lysS [H]

Synonym: Reut_A1070

Alternate gene names: 73540772

Gene position: 1161281-1159731 (Counterclockwise)

Preceding gene: 73540773

Following gene: 73540771

Centisome position: 30.51

GC content: 64.22

Gene sequence:

>1551_bases
ATGACTGAACCCAACCGCGCCCAGGCCGCACCGGCCTCTCCGACCGCAGAGCTGCCCGCCGCAGACGAGAACAAGATCAT
CGCCGAGCGTCGCGAGAAACTGGCGGCCCTGCGCCGGCAAGGCGTGGCCTTCCCCAACGATTTCCGCCCGACCCACCAGG
CCGCCGCGCTGCACGAGCAGTACGGCGAGACCGACCAGCCCGCGCTGGAAGCGACCCCGGTGGAAGTCGCCATTGCCGGC
CGCATGATGCTCAAGCGCGTCATGGGCAAGGCAAGCTTCGCCACCGTGCAGGATGGCAGCGGCCAGATCCAGTTCTACAT
CACGCGCGACAAGGTTGGTGAAGACGTCTACGCCGCCTTCAAGCACTGGGACCTTGGCGACATCATCAGCGCGCGCGGCG
TGCTGTTCCGCACCAACAAGGGCGAGCTGTCCGTGCAGGTGCAGGAACTGCGCCTGCTGTCCAAGTCGCTGCGCCCGCTG
CCGGACAAGTTCCACGGCCTGGCCGACCAGGAAATGAAGTACCGCCAGCGCTATGTCGACCTGATCGTCTCGCCGGAAAC
CCGCAACACCTTCCGCGCGCGCACCAAGGCCATGTCATCGCTGCGCCGCCATATGTCCGACGCCGGCTTCATGGAAGTCG
AAACGCCGATGCTGCACCCGATCCCGGGCGGCGCCGCGGCCAAGCCGTTCATCACGCACCACAATGCGCTGGACATGCAG
ATGTTCATGCGCATCGCGCCGGAGTTGTACCTGAAGCGCCTGATCGTCGGCGGCTTCGAGCGCGTGTTCGAGATCAACCG
CAACTTCCGCAATGAAGGGGTGAGCCCGCGCCACAACCCCGAGTTCACGATGATGGAGTTCTACGCGGCCTACACGGACT
ACCGCTGGCTGATGGACTTTACCGAGAACCTGATCCGCCAGGCCGCCATCGACGCCAGCGGTACCGCGGTGCTGACCTAC
CAGGGCCGCGAGCTGGACTTGTCGAAGCCGTTCCACCGCCTGACCATCTGCCAGGCGATCCAAAAGTTCGCGCCGCAATA
CACCGACGCGCAACTGGCCGATGCCGATTTCCTGCGCACCGAGCTGAAGAAATTTGGCGTGAACACCAGCGCGCCGCAGT
TCCTCAATGCAGGCATCGGTACGCTGCAACTCGTGCTGTTCGAGGAGACCGCGGAAAGCCAGCTGTGGGAGCCGACTTTC
ATCGTCGATTACCCGGTGGAAGTCTCGCCGCTGGCACGTGCATCGGACACAGTGCCGGGCATCACCGAACGCTTCGAGCT
GTTCATCACGGGCCGCGAGATCGCCAACGGCTTCTCGGAGCTCAACGACGCAGAAGACCAGGCCGATCGTTTCCGCAAGC
AGGTCGAGCAGAAGGACGCCGGCGATGAAGAGGCAATGTACTACGACGCCGACTACATTCGCGCGCTGGAATACGGCATG
CCCCCGACGGGCGGCTGCGGTATCGGTATCGACCGACTGGTGATGCTGCTGACGGACAGCCCGAACATCCGCGACGTCAT
TCTGTTCCCGCACCTGCGCCGCGAAGACTGA

Upstream 100 bases:

>100_bases
TCGCCCGGACTTCGGCAGCACCGAGCTGGACGACCTGCGCAATTACTGAAGCCATACTGAACCCGCGCGCGGTACGCCGC
GTGCGATCCGACAAGACATC

Downstream 100 bases:

>100_bases
GCGCTGAGCGCGGCCGCAAGGAAATACGACGCTGAACAGCAAGAACCCCGCCTCTATGGCGGGGTTTTTCTTTTGATCCG
ATGATCGGGAATATTAAAAG

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MTEPNRAQAAPASPTAELPAADENKIIAERREKLAALRRQGVAFPNDFRPTHQAAALHEQYGETDQPALEATPVEVAIAG
RMMLKRVMGKASFATVQDGSGQIQFYITRDKVGEDVYAAFKHWDLGDIISARGVLFRTNKGELSVQVQELRLLSKSLRPL
PDKFHGLADQEMKYRQRYVDLIVSPETRNTFRARTKAMSSLRRHMSDAGFMEVETPMLHPIPGGAAAKPFITHHNALDMQ
MFMRIAPELYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTENLIRQAAIDASGTAVLTY
QGRELDLSKPFHRLTICQAIQKFAPQYTDAQLADADFLRTELKKFGVNTSAPQFLNAGIGTLQLVLFEETAESQLWEPTF
IVDYPVEVSPLARASDTVPGITERFELFITGREIANGFSELNDAEDQADRFRKQVEQKDAGDEEAMYYDADYIRALEYGM
PPTGGCGIGIDRLVMLLTDSPNIRDVILFPHLRRED

Sequences:

>Translated_516_residues
MTEPNRAQAAPASPTAELPAADENKIIAERREKLAALRRQGVAFPNDFRPTHQAAALHEQYGETDQPALEATPVEVAIAG
RMMLKRVMGKASFATVQDGSGQIQFYITRDKVGEDVYAAFKHWDLGDIISARGVLFRTNKGELSVQVQELRLLSKSLRPL
PDKFHGLADQEMKYRQRYVDLIVSPETRNTFRARTKAMSSLRRHMSDAGFMEVETPMLHPIPGGAAAKPFITHHNALDMQ
MFMRIAPELYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTENLIRQAAIDASGTAVLTY
QGRELDLSKPFHRLTICQAIQKFAPQYTDAQLADADFLRTELKKFGVNTSAPQFLNAGIGTLQLVLFEETAESQLWEPTF
IVDYPVEVSPLARASDTVPGITERFELFITGREIANGFSELNDAEDQADRFRKQVEQKDAGDEEAMYYDADYIRALEYGM
PPTGGCGIGIDRLVMLLTDSPNIRDVILFPHLRRED
>Mature_515_residues
TEPNRAQAAPASPTAELPAADENKIIAERREKLAALRRQGVAFPNDFRPTHQAAALHEQYGETDQPALEATPVEVAIAGR
MMLKRVMGKASFATVQDGSGQIQFYITRDKVGEDVYAAFKHWDLGDIISARGVLFRTNKGELSVQVQELRLLSKSLRPLP
DKFHGLADQEMKYRQRYVDLIVSPETRNTFRARTKAMSSLRRHMSDAGFMEVETPMLHPIPGGAAAKPFITHHNALDMQM
FMRIAPELYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTENLIRQAAIDASGTAVLTYQ
GRELDLSKPFHRLTICQAIQKFAPQYTDAQLADADFLRTELKKFGVNTSAPQFLNAGIGTLQLVLFEETAESQLWEPTFI
VDYPVEVSPLARASDTVPGITERFELFITGREIANGFSELNDAEDQADRFRKQVEQKDAGDEEAMYYDADYIRALEYGMP
PTGGCGIGIDRLVMLLTDSPNIRDVILFPHLRRED

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=509, Percent_Identity=39.2927308447937, Blast_Score=353, Evalue=3e-97,
Organism=Homo sapiens, GI5031815, Length=509, Percent_Identity=39.2927308447937, Blast_Score=353, Evalue=3e-97,
Organism=Homo sapiens, GI40789249, Length=245, Percent_Identity=23.6734693877551, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1789256, Length=495, Percent_Identity=59.3939393939394, Blast_Score=609, Evalue=1e-175,
Organism=Escherichia coli, GI1790571, Length=495, Percent_Identity=59.1919191919192, Blast_Score=609, Evalue=1e-175,
Organism=Escherichia coli, GI87082379, Length=326, Percent_Identity=31.9018404907975, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1788173, Length=548, Percent_Identity=22.2627737226277, Blast_Score=106, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17535927, Length=461, Percent_Identity=42.5162689804772, Blast_Score=355, Evalue=5e-98,
Organism=Caenorhabditis elegans, GI17535925, Length=461, Percent_Identity=42.5162689804772, Blast_Score=354, Evalue=5e-98,
Organism=Caenorhabditis elegans, GI71994340, Length=463, Percent_Identity=42.548596112311, Blast_Score=353, Evalue=9e-98,
Organism=Caenorhabditis elegans, GI32566633, Length=246, Percent_Identity=24.7967479674797, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6320242, Length=526, Percent_Identity=40.4942965779468, Blast_Score=347, Evalue=2e-96,
Organism=Saccharomyces cerevisiae, GI6324256, Length=572, Percent_Identity=29.8951048951049, Blast_Score=180, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24640849, Length=460, Percent_Identity=42.8260869565217, Blast_Score=352, Evalue=4e-97,
Organism=Drosophila melanogaster, GI24640851, Length=460, Percent_Identity=42.8260869565217, Blast_Score=351, Evalue=5e-97,
Organism=Drosophila melanogaster, GI24584738, Length=292, Percent_Identity=22.6027397260274, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 58357; Mature: 58226

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPNRAQAAPASPTAELPAADENKIIAERREKLAALRRQGVAFPNDFRPTHQAAALHEQ
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
YGETDQPALEATPVEVAIAGRMMLKRVMGKASFATVQDGSGQIQFYITRDKVGEDVYAAF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCHHHHHHH
KHWDLGDIISARGVLFRTNKGELSVQVQELRLLSKSLRPLPDKFHGLADQEMKYRQRYVD
HCCCHHHHHHHCCEEEECCCCCEEEEHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHH
LIVSPETRNTFRARTKAMSSLRRHMSDAGFMEVETPMLHPIPGGAAAKPFITHHNALDMQ
EEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCEEECCCHHHHH
MFMRIAPELYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TENLIRQAAIDASGTAVLTYQGRELDLSKPFHRLTICQAIQKFAPQYTDAQLADADFLRT
HHHHHHHHHCCCCCCEEEEECCCEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
ELKKFGVNTSAPQFLNAGIGTLQLVLFEETAESQLWEPTFIVDYPVEVSPLARASDTVPG
HHHHHCCCCCCCHHHHCCCCCEEEEEEECHHHHHCCCCEEEEECCCCCCCCHHCCCCCCC
ITERFELFITGREIANGFSELNDAEDQADRFRKQVEQKDAGDEEAMYYDADYIRALEYGM
HHHEEEEEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHCCC
PPTGGCGIGIDRLVMLLTDSPNIRDVILFPHLRRED
CCCCCCCCCHHHEEEEEECCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
TEPNRAQAAPASPTAELPAADENKIIAERREKLAALRRQGVAFPNDFRPTHQAAALHEQ
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
YGETDQPALEATPVEVAIAGRMMLKRVMGKASFATVQDGSGQIQFYITRDKVGEDVYAAF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCHHHHHHH
KHWDLGDIISARGVLFRTNKGELSVQVQELRLLSKSLRPLPDKFHGLADQEMKYRQRYVD
HCCCHHHHHHHCCEEEECCCCCEEEEHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHH
LIVSPETRNTFRARTKAMSSLRRHMSDAGFMEVETPMLHPIPGGAAAKPFITHHNALDMQ
EEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCEEECCCHHHHH
MFMRIAPELYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TENLIRQAAIDASGTAVLTYQGRELDLSKPFHRLTICQAIQKFAPQYTDAQLADADFLRT
HHHHHHHHHCCCCCCEEEEECCCEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
ELKKFGVNTSAPQFLNAGIGTLQLVLFEETAESQLWEPTFIVDYPVEVSPLARASDTVPG
HHHHHCCCCCCCHHHHCCCCCEEEEEEECHHHHHCCCCEEEEECCCCCCCCHHCCCCCCC
ITERFELFITGREIANGFSELNDAEDQADRFRKQVEQKDAGDEEAMYYDADYIRALEYGM
HHHEEEEEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHCCC
PPTGGCGIGIDRLVMLLTDSPNIRDVILFPHLRRED
CCCCCCCCCHHHEEEEEECCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA