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The map label for this gene is 73540752

Identifier: 73540752

GI number: 73540752

Start: 1141057

End: 1142298

Strand: Reverse

Name: 73540752

Synonym: Reut_A1049

Alternate gene names: NA

Gene position: 1142298-1141057 (Counterclockwise)

Preceding gene: 73540753

Following gene: 73540750

Centisome position: 30.01

GC content: 62.88

Gene sequence:

>1242_bases
ATGCTCTACCAACTGCATGAGTTCCAGCGTTCGATGCTGCACCCGCTGACCGCGTGGGCACAGGCGACCGCCAAGACTTT
CACCAATCCCCTCAGTCCGTTGTCCCTGGTTCCCGGCGCCCCCCGCCTGGCTGCCGGCTACGAACTCCTGTACAGGCTCG
GAAAGGAATACGAGAAACCCGCGTTCGACATCAAGTCGGTACGCTCGAACGGCCGCGAAATCCCGATCGTCGAACAGACC
ATCATGGAAAAGCCGTTCTGCCGCCTGGTCCGCTTCAAGCGCTACGCCGATGATCCGGACACCATCAAGCTGCTCAAGGA
CGAGCCCGTGGTGCTGGTCGCCGCACCGCTGTCGGGCCACCACGCCACGCTTCTGCGCGACACGGTGCGCACGCTGCTGC
AGGACCACAAGGTCTATGTGACCGACTGGGTCGACGCGCGCATGGTGCCGGCCGAACATGGCCCGTTCCACCTGTCGGAC
TACATTTATTACATTCAGGAGTTCATTCGCCATATCGGCGCGGACAAGCTCCACGTCATTTCGGTGTGCCAGCCCACCGT
GCCGGTGCTGGCAGCGATTTCGCTGATGGCCTCGGCGGGCGAGAAGACGCCGCTCACCATGACCATGATGGGTGGCCCGA
TCGACGCTCGCAAGAGCCCGACAGCGGTCAACTCGCTTGCGACCAACAAGTCGTTCGAGTGGTTCGAGAACAACGTGATC
TACACCGTGCCGGCCAACTATCCCGGCCACGGGCGCCGTGTCTATCCGGGCTTCCTGCAGCATGCCGGTTTCGTGGCGAT
GAACCCGGACCGCCACCTGTCGTCGCACTACGACTACTACCTGAGCCTGATCCAGGGGGATGCCGATGACGCGGAAGCTC
ACGTGCGGTTCTACGACGAGTACAACGCCGTGCTCGACATGGCAGCCGAGTACTATCTCGACACGATTCGCGAGGTCTTC
CAGGAGTTCCGCCTGGCCAACGGCACGTGGACCATCGACGGCAATCCGGTGCGCCCGCAAGACATCAAGGGCACTGCGCT
GCTGACCGTCGAAGGCGAGCTCGACGACATCTCCGGCGCCGGCCAGACCTCCGCGGCGCACGACTTGTGCGCAGGCATTC
CACAGACGCACAAGCACCATCTGTCCGCAGCGAAGTGCGGCCACTACGGGATCTTCTCGGGCCGCCGCTGGCGCGATGAA
ATCTATCCGCAGCTGCGCGATTTCATCCGCAAGTACCGGTGA

Upstream 100 bases:

>100_bases
CGATCGTTCGGTTGCGTGCATAATCGGCACACAGTCGCACGGTGGTGCCCCCATCGAGAAAGACGTTTTCATCGTATTCG
TATCACCAGGCCAGACCGCC

Downstream 100 bases:

>100_bases
CCTGACCGTCCGCCATGCAAGAACGCCCGCAACCGCGGGCGTTTTTTTCTGCGCCGTGGATGCAACGCGCCGGTCAGCGC
CGCAGGTAGCTCAGGAGCAT

Product: polyhydroxyalkanoate depolymerase

Products: NA

Alternate protein names: Intracellular PHB Depolymerase; Polyhydroxyalkanoate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase; PHB Depolymerase; Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Intracellular Poly(3-Hydroxybutyrate) Depolymerase; Intracellular Polyhydroxyalkanoate Depolymerase; PHB De-Polymerase Domain-Containing Protein; Polyhydroxyalkanoate Depolymerase Intracellular Subfamily; Poly(3-Hydroxyalkanoate) Depolymerase; Poly(3-Hydroxybutyrate) Depolymerase; Alpha/Beta Hydrolase Fold; Intracellular PHB Depolymerase Protein; Poly-Beta-Hydroxyalkanoate Depolymerase-Like Protein; PHB De-Polymerase Domain Protein; Intracellular Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Poly[D(-)-3-Hydroxybutyrate](Phb) Depolymerase; Poly-Beta-Hydroxyalkanoate/Butyrate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase Protein; Poly[D(-)-3-Hydroxyalkanoate] Depolymerase; Polyhydroxybutanoate Depolymerase; Poly-Beta-Hydroxybutyrate Depolymerase

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MLYQLHEFQRSMLHPLTAWAQATAKTFTNPLSPLSLVPGAPRLAAGYELLYRLGKEYEKPAFDIKSVRSNGREIPIVEQT
IMEKPFCRLVRFKRYADDPDTIKLLKDEPVVLVAAPLSGHHATLLRDTVRTLLQDHKVYVTDWVDARMVPAEHGPFHLSD
YIYYIQEFIRHIGADKLHVISVCQPTVPVLAAISLMASAGEKTPLTMTMMGGPIDARKSPTAVNSLATNKSFEWFENNVI
YTVPANYPGHGRRVYPGFLQHAGFVAMNPDRHLSSHYDYYLSLIQGDADDAEAHVRFYDEYNAVLDMAAEYYLDTIREVF
QEFRLANGTWTIDGNPVRPQDIKGTALLTVEGELDDISGAGQTSAAHDLCAGIPQTHKHHLSAAKCGHYGIFSGRRWRDE
IYPQLRDFIRKYR

Sequences:

>Translated_413_residues
MLYQLHEFQRSMLHPLTAWAQATAKTFTNPLSPLSLVPGAPRLAAGYELLYRLGKEYEKPAFDIKSVRSNGREIPIVEQT
IMEKPFCRLVRFKRYADDPDTIKLLKDEPVVLVAAPLSGHHATLLRDTVRTLLQDHKVYVTDWVDARMVPAEHGPFHLSD
YIYYIQEFIRHIGADKLHVISVCQPTVPVLAAISLMASAGEKTPLTMTMMGGPIDARKSPTAVNSLATNKSFEWFENNVI
YTVPANYPGHGRRVYPGFLQHAGFVAMNPDRHLSSHYDYYLSLIQGDADDAEAHVRFYDEYNAVLDMAAEYYLDTIREVF
QEFRLANGTWTIDGNPVRPQDIKGTALLTVEGELDDISGAGQTSAAHDLCAGIPQTHKHHLSAAKCGHYGIFSGRRWRDE
IYPQLRDFIRKYR
>Mature_413_residues
MLYQLHEFQRSMLHPLTAWAQATAKTFTNPLSPLSLVPGAPRLAAGYELLYRLGKEYEKPAFDIKSVRSNGREIPIVEQT
IMEKPFCRLVRFKRYADDPDTIKLLKDEPVVLVAAPLSGHHATLLRDTVRTLLQDHKVYVTDWVDARMVPAEHGPFHLSD
YIYYIQEFIRHIGADKLHVISVCQPTVPVLAAISLMASAGEKTPLTMTMMGGPIDARKSPTAVNSLATNKSFEWFENNVI
YTVPANYPGHGRRVYPGFLQHAGFVAMNPDRHLSSHYDYYLSLIQGDADDAEAHVRFYDEYNAVLDMAAEYYLDTIREVF
QEFRLANGTWTIDGNPVRPQDIKGTALLTVEGELDDISGAGQTSAAHDLCAGIPQTHKHHLSAAKCGHYGIFSGRRWRDE
IYPQLRDFIRKYR

Specific function: Unknown

COG id: COG4553

COG function: function code I; Poly-beta-hydroxyalkanoate depolymerase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 46704; Mature: 46704

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYQLHEFQRSMLHPLTAWAQATAKTFTNPLSPLSLVPGAPRLAAGYELLYRLGKEYEKP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHCCC
AFDIKSVRSNGREIPIVEQTIMEKPFCRLVRFKRYADDPDTIKLLKDEPVVLVAAPLSGH
CHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCCCC
HATLLRDTVRTLLQDHKVYVTDWVDARMVPAEHGPFHLSDYIYYIQEFIRHIGADKLHVI
HHHHHHHHHHHHHHCCCEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
SVCQPTVPVLAAISLMASAGEKTPLTMTMMGGPIDARKSPTAVNSLATNKSFEWFENNVI
EECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHCCCCCHHHCCCEE
YTVPANYPGHGRRVYPGFLQHAGFVAMNPDRHLSSHYDYYLSLIQGDADDAEAHVRFYDE
EEECCCCCCCCCEECCHHHHHCCEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHEEEHHH
YNAVLDMAAEYYLDTIREVFQEFRLANGTWTIDGNPVRPQDIKGTALLTVEGELDDISGA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEEECCCCCCCCC
GQTSAAHDLCAGIPQTHKHHLSAAKCGHYGIFSGRRWRDEIYPQLRDFIRKYR
CCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLYQLHEFQRSMLHPLTAWAQATAKTFTNPLSPLSLVPGAPRLAAGYELLYRLGKEYEKP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHCCC
AFDIKSVRSNGREIPIVEQTIMEKPFCRLVRFKRYADDPDTIKLLKDEPVVLVAAPLSGH
CHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCCCC
HATLLRDTVRTLLQDHKVYVTDWVDARMVPAEHGPFHLSDYIYYIQEFIRHIGADKLHVI
HHHHHHHHHHHHHHCCCEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
SVCQPTVPVLAAISLMASAGEKTPLTMTMMGGPIDARKSPTAVNSLATNKSFEWFENNVI
EECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHCCCCCHHHCCCEE
YTVPANYPGHGRRVYPGFLQHAGFVAMNPDRHLSSHYDYYLSLIQGDADDAEAHVRFYDE
EEECCCCCCCCCEECCHHHHHCCEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHEEEHHH
YNAVLDMAAEYYLDTIREVFQEFRLANGTWTIDGNPVRPQDIKGTALLTVEGELDDISGA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEEECCCCCCCCC
GQTSAAHDLCAGIPQTHKHHLSAAKCGHYGIFSGRRWRDEIYPQLRDFIRKYR
CCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA