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The map label for this gene is braD [H]

Identifier: 73540690

GI number: 73540690

Start: 1075748

End: 1077409

Strand: Direct

Name: braD [H]

Synonym: Reut_A0987

Alternate gene names: 73540690

Gene position: 1075748-1077409 (Clockwise)

Preceding gene: 73540689

Following gene: 73540691

Centisome position: 28.26

GC content: 65.46

Gene sequence:

>1662_bases
ATGTCCCAATCGTTACCGAAGATCGCCATGTCGTGGCTGCGCGCCATGCTGGCTGCGCTGCTGCTTGCCGTGCCGCTATT
GGCGGCAGCCGCAGGCGCATTGACGCAAGCCGACCTCAAGCCATTGGCCGAGGACGATTTCGATGCCAAGACCGCGGCCT
TGTCCGCGCTGGCCAAGGCGCCGCCGGAGCAGGCCGGGCCTATTCTCAAGGCGTTGCAGGACGATACGCTGCAATATGCC
CCATCGGTGGGCATGGTGCGCCAGGACGGTGACAAGACCGTCGATGCGATCACCGGCAAGCCAGTGACGGTCAAGGCCGA
TGAACTGGAATCGCTGACCCTGAACAACAGCCTGCGCGCGATCGTCGATAGCGCCGCAAGCGGATTCCTGCTGCAGTCGC
CCGATATCTCGCTGCGCACGCAGGCGATCAACACGCTGTACGATCAACCAGAGAATGCGTCGCGCGCCAGTGTGGAAACT
GCGCGCAAGTCTGAGGCCGATCCCGGCCTGCGCGCACGCCTGGACGTGATCTGGGCCACTACGGTGCTAGCGGAGGAGCT
GAAGGCTGGCGATGCCGCGCAGCGCGAGGGGCGTCTCGAAGCCATTCGCGTCCTCGGCCGCGACAGCAATCCGCAAAGCC
GCCAGAAGCTCGCACCGTTAGTCGAGCGCGGTAGCGACGGCAAGTATCGCGAACCCGATGTCGAGGTACGTACCGCCGCG
CAGCAGGCGCTCGACACGCTGCGCAGCGACCAGCGCCGCGCCGAACTGGTCGGCAACCTGTTCGCAGGCCTGAGCCTTGG
CAGCGTGCTATTGCTGGCCGCGCTGGGCCTGGCGATCACCTACGGCCTGATCGGCGTCATCAACATGGCGCACGGCGAAT
TCCTGATGATCGGCGCCTATGCGACCTACGTGGTGCAGACGCTGTTCCGCACGTATGCGCCCGGTGCGTTCGACTGGTAC
CTGCCCGCGGCACTGCCGGCATCGTTCCTCGCGGCCGGCATCGTCGGCTTCGTGCTCGAGCGGCTGGTGCTGCGCCATCT
CTATGGCCGCCCGCTGGAGACCTTGCTCGCCACGTTCGGTGTGAGCCTGCTGCTGATGCAGGCGGTGCGCACGTTGTTCG
GCGCGCAGAACGTTGAGGTGGCCAACCCCGCGTGGATGAGCGGCGGTGTCGAATGGATGCCGGGCCTTGTGATTCCGTAC
AACCGCATCGTGATCATCCTGTTCGCGCTGGCCGTCGTGGCCGTTGCCTGGGCGGTGCTCAACCGTACGCGACTCGGCCT
GTTCGTACGTGCCACGACACAGAACCGGACCATGGCTGCGTGCGTGGGCGTGCGCACGTGGAAGGTCGACAGCTACGCGT
TCGCGTTCGGTGCGGGCATTGCCGGCCTGGGCGGCTGCGCGCTGTCGCAGATCGGCAATGTCGGCCCGGACCTCGGCCAG
GCCTACATCATCGATTCCTTCATGGTGGTGGTGCTGGGCGGGGTAGGGCAGTTGGCAGGCACTATCGTCGGCGCGTTCGG
CCTCGGCATCATCAACAAGTTCATCGAGCCGTTCTATGGCGCGGTGCTCGCCAAGATCTTCGTCCTGGTGCTGATCGTGC
TTTTCATCCAGAAGCGCCCGCAGGGACTTTTCGCGCTCAAGGGGCGCAGCGCGGAGGCATGA

Upstream 100 bases:

>100_bases
GCTGACCGTTGCCGGCTGGCGGTGGTGCGCGCCTGACCGGGCCTGGGGGGGTCCCCGAAAAAAGCAGGCACGACCACGTG
CCGCCCAATCGCCAGCACCT

Downstream 100 bases:

>100_bases
TGCAGAGCTTTCACTCAAACTCCCCGGGCACGCCGTTTCGTCTGTCGGTGCCCGAGCGCCAGCCGCTGTTCACTGGGCGC
GGCTGGGCTGTGCTGGCGAT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MSQSLPKIAMSWLRAMLAALLLAVPLLAAAAGALTQADLKPLAEDDFDAKTAALSALAKAPPEQAGPILKALQDDTLQYA
PSVGMVRQDGDKTVDAITGKPVTVKADELESLTLNNSLRAIVDSAASGFLLQSPDISLRTQAINTLYDQPENASRASVET
ARKSEADPGLRARLDVIWATTVLAEELKAGDAAQREGRLEAIRVLGRDSNPQSRQKLAPLVERGSDGKYREPDVEVRTAA
QQALDTLRSDQRRAELVGNLFAGLSLGSVLLLAALGLAITYGLIGVINMAHGEFLMIGAYATYVVQTLFRTYAPGAFDWY
LPAALPASFLAAGIVGFVLERLVLRHLYGRPLETLLATFGVSLLLMQAVRTLFGAQNVEVANPAWMSGGVEWMPGLVIPY
NRIVIILFALAVVAVAWAVLNRTRLGLFVRATTQNRTMAACVGVRTWKVDSYAFAFGAGIAGLGGCALSQIGNVGPDLGQ
AYIIDSFMVVVLGGVGQLAGTIVGAFGLGIINKFIEPFYGAVLAKIFVLVLIVLFIQKRPQGLFALKGRSAEA

Sequences:

>Translated_553_residues
MSQSLPKIAMSWLRAMLAALLLAVPLLAAAAGALTQADLKPLAEDDFDAKTAALSALAKAPPEQAGPILKALQDDTLQYA
PSVGMVRQDGDKTVDAITGKPVTVKADELESLTLNNSLRAIVDSAASGFLLQSPDISLRTQAINTLYDQPENASRASVET
ARKSEADPGLRARLDVIWATTVLAEELKAGDAAQREGRLEAIRVLGRDSNPQSRQKLAPLVERGSDGKYREPDVEVRTAA
QQALDTLRSDQRRAELVGNLFAGLSLGSVLLLAALGLAITYGLIGVINMAHGEFLMIGAYATYVVQTLFRTYAPGAFDWY
LPAALPASFLAAGIVGFVLERLVLRHLYGRPLETLLATFGVSLLLMQAVRTLFGAQNVEVANPAWMSGGVEWMPGLVIPY
NRIVIILFALAVVAVAWAVLNRTRLGLFVRATTQNRTMAACVGVRTWKVDSYAFAFGAGIAGLGGCALSQIGNVGPDLGQ
AYIIDSFMVVVLGGVGQLAGTIVGAFGLGIINKFIEPFYGAVLAKIFVLVLIVLFIQKRPQGLFALKGRSAEA
>Mature_552_residues
SQSLPKIAMSWLRAMLAALLLAVPLLAAAAGALTQADLKPLAEDDFDAKTAALSALAKAPPEQAGPILKALQDDTLQYAP
SVGMVRQDGDKTVDAITGKPVTVKADELESLTLNNSLRAIVDSAASGFLLQSPDISLRTQAINTLYDQPENASRASVETA
RKSEADPGLRARLDVIWATTVLAEELKAGDAAQREGRLEAIRVLGRDSNPQSRQKLAPLVERGSDGKYREPDVEVRTAAQ
QALDTLRSDQRRAELVGNLFAGLSLGSVLLLAALGLAITYGLIGVINMAHGEFLMIGAYATYVVQTLFRTYAPGAFDWYL
PAALPASFLAAGIVGFVLERLVLRHLYGRPLETLLATFGVSLLLMQAVRTLFGAQNVEVANPAWMSGGVEWMPGLVIPYN
RIVIILFALAVVAVAWAVLNRTRLGLFVRATTQNRTMAACVGVRTWKVDSYAFAFGAGIAGLGGCALSQIGNVGPDLGQA
YIIDSFMVVVLGGVGQLAGTIVGAFGLGIINKFIEPFYGAVLAKIFVLVLIVLFIQKRPQGLFALKGRSAEA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=291, Percent_Identity=27.8350515463918, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 58786; Mature: 58655

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSLPKIAMSWLRAMLAALLLAVPLLAAAAGALTQADLKPLAEDDFDAKTAALSALAKA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHC
PPEQAGPILKALQDDTLQYAPSVGMVRQDGDKTVDAITGKPVTVKADELESLTLNNSLRA
CCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHCCCCCEEEEHHHHHHHHHCCHHHH
IVDSAASGFLLQSPDISLRTQAINTLYDQPENASRASVETARKSEADPGLRARLDVIWAT
HHHHHCCCCEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
TVLAEELKAGDAAQREGRLEAIRVLGRDSNPQSRQKLAPLVERGSDGKYREPDVEVRTAA
HHHHHHHHCCCHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
QQALDTLRSDQRRAELVGNLFAGLSLGSVLLLAALGLAITYGLIGVINMAHGEFLMIGAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHH
ATYVVQTLFRTYAPGAFDWYLPAALPASFLAAGIVGFVLERLVLRHLYGRPLETLLATFG
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
VSLLLMQAVRTLFGAQNVEVANPAWMSGGVEWMPGLVIPYNRIVIILFALAVVAVAWAVL
HHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHCCCEECCHHHHHHHHHHHHHHHHHHHHH
NRTRLGLFVRATTQNRTMAACVGVRTWKVDSYAFAFGAGIAGLGGCALSQIGNVGPDLGQ
HHHHHEEEEEECCCCCHHHHHHCCEEEEECCHHHHHHCCHHHHHHHHHHHHCCCCCCCCH
AYIIDSFMVVVLGGVGQLAGTIVGAFGLGIINKFIEPFYGAVLAKIFVLVLIVLFIQKRP
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QGLFALKGRSAEA
CCEEEECCCCCCC
>Mature Secondary Structure 
SQSLPKIAMSWLRAMLAALLLAVPLLAAAAGALTQADLKPLAEDDFDAKTAALSALAKA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHC
PPEQAGPILKALQDDTLQYAPSVGMVRQDGDKTVDAITGKPVTVKADELESLTLNNSLRA
CCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHCCCCCEEEEHHHHHHHHHCCHHHH
IVDSAASGFLLQSPDISLRTQAINTLYDQPENASRASVETARKSEADPGLRARLDVIWAT
HHHHHCCCCEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
TVLAEELKAGDAAQREGRLEAIRVLGRDSNPQSRQKLAPLVERGSDGKYREPDVEVRTAA
HHHHHHHHCCCHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
QQALDTLRSDQRRAELVGNLFAGLSLGSVLLLAALGLAITYGLIGVINMAHGEFLMIGAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHH
ATYVVQTLFRTYAPGAFDWYLPAALPASFLAAGIVGFVLERLVLRHLYGRPLETLLATFG
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
VSLLLMQAVRTLFGAQNVEVANPAWMSGGVEWMPGLVIPYNRIVIILFALAVVAVAWAVL
HHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHCCCEECCHHHHHHHHHHHHHHHHHHHHH
NRTRLGLFVRATTQNRTMAACVGVRTWKVDSYAFAFGAGIAGLGGCALSQIGNVGPDLGQ
HHHHHEEEEEECCCCCHHHHHHCCEEEEECCHHHHHHCCHHHHHHHHHHHHCCCCCCCCH
AYIIDSFMVVVLGGVGQLAGTIVGAFGLGIINKFIEPFYGAVLAKIFVLVLIVLFIQKRP
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QGLFALKGRSAEA
CCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]