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The map label for this gene is nuoF2 [H]

Identifier: 73540669

GI number: 73540669

Start: 1052763

End: 1054058

Strand: Direct

Name: nuoF2 [H]

Synonym: Reut_A0966

Alternate gene names: 73540669

Gene position: 1052763-1054058 (Clockwise)

Preceding gene: 73540668

Following gene: 73540670

Centisome position: 27.66

GC content: 62.81

Gene sequence:

>1296_bases
ATGACCTCTCTGCACGACCGCCACATCCAGCCGCTGATCCTGGCCGGCCTGGACGGCAAGAACTGGCACCTGGAAGACTA
CGTCAAGCGCGGCGGCTACCAGCAGCTCAAGCGCATCCTGACCGAAAAGATCCCGCCCGAGCAGGTGATCGCGGACGTCA
AGGCCTCGGGCCTGCGTGGCCGTGGGGGCGCGGGCTTCCCGACCGGCCTGAAGTGGAGCTTCATGCCGCGTACGTTTCCG
GGCCAGAAGTACCTCGTCTGCAATACCGATGAGGGCGAGCCCGGTACGTTCAAGGACCGCGACATCATCCGGTTCAATCC
GCACTCGCTGATCGAGGGCATGGCCATCGGCGCTTACGCGATGGGCATCACCGTGGGCTACAACTATATCCACGGCGAGA
TCTGGAACGAATACAAGATCTTCGAGGAAGCCCTCGAAGAGGCGCGCGCCGCGGGTTTCCTCGGCGACAACATCCTGGGC
TCGGGCTTCAGCTTCCAGCTGCACGCCCACCATGGCTATGGCGCGTACATCTGCGGCGAAGAAACCGCGCTGCTCGAATC
GCTGGAAGGCAAGAAGGGCCAGCCGCGTTTCAAGCCGCCCTTCCCGGCGAGCTTCGGCCTGTACGGCAAGCCGACCACCA
TCAATAACACCGAGACGTTTGCCGCGGTGCCGTTCCTGCTGGCCATCGGCCCGGAGAACTACCTGAAGATGGGCAAGCCG
AACAACGGCGGAACCAAGATCTTCTCGATCTCCGGTGACGTCGAGCGTCCGGGCAACTACGAGATCCCGCTGGGCACGCC
GTTCGCCAAGCTGCTGGAGCTGGCTGGTGGCATGCGCGGTGGCAAGCGCATCAAGGCCGTGATTCCCGGTGGTTCGTCCG
CGCCGGTCGTACCGGGCGAGATGATGATGGCGTCCGACATGGACTACGACTCGATCGCCAAGGCCGGCTCGATGCTGGGC
TCGGGCGCCGTGATCGTGATGGACGAGACGCGCTGCATGGTGCGCTCGTTGCTGCGCCTGTCGTATTTCTATTTTGAAGA
ATCGTGTGGCCAGTGCACGCCGTGCCGTGAAGGCACCGGTTGGCTGTACCGCATGGTCAATCGCATTGAACACGGAGAGG
GGCGCCAGGAAGACCTGGACCTGCTCAACAACGTCGCGGAAAACATCATGGGCCGCACCATCTGCGCGCTCGGCGATGCC
GCGGCGATGCCGGTCCGCGGCATGCTCAAGCACTACTGGAAAGAGTTCGAATATCACGTCGAACACAAGCAGTGCATGGT
TCCGGCCTACATCTAA

Upstream 100 bases:

>100_bases
GTCAACAACACCCGCATGTGCAGCTTCATGAGCGACGACAAGCTCGACGCGCTGGTCGACGAGCTGAAGGCCGACGCTGC
CGCCAAGGGGGCAAAGTAAC

Downstream 100 bases:

>100_bases
GCGTGGCAGAACATGGTTGAACTCGAGATCGACGGCAAGAAGGTTGAGGTTGCAGAAGGCAGCCTGGTGATGGAAGCCGC
CCGCAAGCTGGGCACCTACA

Product: NADH-quinone oxidoreductase, F subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTSLHDRHIQPLILAGLDGKNWHLEDYVKRGGYQQLKRILTEKIPPEQVIADVKASGLRGRGGAGFPTGLKWSFMPRTFP
GQKYLVCNTDEGEPGTFKDRDIIRFNPHSLIEGMAIGAYAMGITVGYNYIHGEIWNEYKIFEEALEEARAAGFLGDNILG
SGFSFQLHAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTETFAAVPFLLAIGPENYLKMGKP
NNGGTKIFSISGDVERPGNYEIPLGTPFAKLLELAGGMRGGKRIKAVIPGGSSAPVVPGEMMMASDMDYDSIAKAGSMLG
SGAVIVMDETRCMVRSLLRLSYFYFEESCGQCTPCREGTGWLYRMVNRIEHGEGRQEDLDLLNNVAENIMGRTICALGDA
AAMPVRGMLKHYWKEFEYHVEHKQCMVPAYI

Sequences:

>Translated_431_residues
MTSLHDRHIQPLILAGLDGKNWHLEDYVKRGGYQQLKRILTEKIPPEQVIADVKASGLRGRGGAGFPTGLKWSFMPRTFP
GQKYLVCNTDEGEPGTFKDRDIIRFNPHSLIEGMAIGAYAMGITVGYNYIHGEIWNEYKIFEEALEEARAAGFLGDNILG
SGFSFQLHAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTETFAAVPFLLAIGPENYLKMGKP
NNGGTKIFSISGDVERPGNYEIPLGTPFAKLLELAGGMRGGKRIKAVIPGGSSAPVVPGEMMMASDMDYDSIAKAGSMLG
SGAVIVMDETRCMVRSLLRLSYFYFEESCGQCTPCREGTGWLYRMVNRIEHGEGRQEDLDLLNNVAENIMGRTICALGDA
AAMPVRGMLKHYWKEFEYHVEHKQCMVPAYI
>Mature_430_residues
TSLHDRHIQPLILAGLDGKNWHLEDYVKRGGYQQLKRILTEKIPPEQVIADVKASGLRGRGGAGFPTGLKWSFMPRTFPG
QKYLVCNTDEGEPGTFKDRDIIRFNPHSLIEGMAIGAYAMGITVGYNYIHGEIWNEYKIFEEALEEARAAGFLGDNILGS
GFSFQLHAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTETFAAVPFLLAIGPENYLKMGKPN
NGGTKIFSISGDVERPGNYEIPLGTPFAKLLELAGGMRGGKRIKAVIPGGSSAPVVPGEMMMASDMDYDSIAKAGSMLGS
GAVIVMDETRCMVRSLLRLSYFYFEESCGQCTPCREGTGWLYRMVNRIEHGEGRQEDLDLLNNVAENIMGRTICALGDAA
AMPVRGMLKHYWKEFEYHVEHKQCMVPAYI

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=425, Percent_Identity=46.5882352941176, Blast_Score=386, Evalue=1e-107,
Organism=Homo sapiens, GI260656005, Length=425, Percent_Identity=46.5882352941176, Blast_Score=385, Evalue=1e-107,
Organism=Escherichia coli, GI1788620, Length=402, Percent_Identity=43.2835820895522, Blast_Score=332, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI17531727, Length=431, Percent_Identity=47.0997679814385, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI19920794, Length=413, Percent_Identity=46.2469733656174, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45550452, Length=415, Percent_Identity=45.0602409638554, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24653819, Length=402, Percent_Identity=36.5671641791045, Blast_Score=258, Evalue=7e-69,
Organism=Drosophila melanogaster, GI24653817, Length=402, Percent_Identity=32.8358208955224, Blast_Score=220, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 47556; Mature: 47425

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLHDRHIQPLILAGLDGKNWHLEDYVKRGGYQQLKRILTEKIPPEQVIADVKASGLRG
CCCCCCCCCCEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCC
RGGAGFPTGLKWSFMPRTFPGQKYLVCNTDEGEPGTFKDRDIIRFNPHSLIEGMAIGAYA
CCCCCCCCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCCCEEEECHHHHHHHHHHHHHH
MGITVGYNYIHGEIWNEYKIFEEALEEARAAGFLGDNILGSGFSFQLHAHHGYGAYICGE
HHHHHCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCEEEEECC
ETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTETFAAVPFLLAIGPENYLKMGKP
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEECCHHHHCCCCC
NNGGTKIFSISGDVERPGNYEIPLGTPFAKLLELAGGMRGGKRIKAVIPGGSSAPVVPGE
CCCCCEEEEECCCCCCCCCEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCH
MMMASDMDYDSIAKAGSMLGSGAVIVMDETRCMVRSLLRLSYFYFEESCGQCTPCREGTG
HHEECCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
WLYRMVNRIEHGEGRQEDLDLLNNVAENIMGRTICALGDAAAMPVRGMLKHYWKEFEYHV
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHH
EHKQCMVPAYI
CHHHEECCCCC
>Mature Secondary Structure 
TSLHDRHIQPLILAGLDGKNWHLEDYVKRGGYQQLKRILTEKIPPEQVIADVKASGLRG
CCCCCCCCCEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCC
RGGAGFPTGLKWSFMPRTFPGQKYLVCNTDEGEPGTFKDRDIIRFNPHSLIEGMAIGAYA
CCCCCCCCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCCCEEEECHHHHHHHHHHHHHH
MGITVGYNYIHGEIWNEYKIFEEALEEARAAGFLGDNILGSGFSFQLHAHHGYGAYICGE
HHHHHCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCEEEEECC
ETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTETFAAVPFLLAIGPENYLKMGKP
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEECCHHHHCCCCC
NNGGTKIFSISGDVERPGNYEIPLGTPFAKLLELAGGMRGGKRIKAVIPGGSSAPVVPGE
CCCCCEEEEECCCCCCCCCEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCH
MMMASDMDYDSIAKAGSMLGSGAVIVMDETRCMVRSLLRLSYFYFEESCGQCTPCREGTG
HHEECCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
WLYRMVNRIEHGEGRQEDLDLLNNVAENIMGRTICALGDAAAMPVRGMLKHYWKEFEYHV
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHH
EHKQCMVPAYI
CHHHEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]