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The map label for this gene is nuoE2 [H]

Identifier: 73540668

GI number: 73540668

Start: 1052255

End: 1052761

Strand: Direct

Name: nuoE2 [H]

Synonym: Reut_A0965

Alternate gene names: 73540668

Gene position: 1052255-1052761 (Clockwise)

Preceding gene: 73540667

Following gene: 73540669

Centisome position: 27.64

GC content: 62.33

Gene sequence:

>507_bases
ATGACCATGCTATCAGCAGAAGCTCTCAAGGAAATCGATCGCGCGATCGCGAAGTATCCGGCCGACCAGAAGCAGTCGGC
CGTGATGGCGGCGCTTGCCGTGGCGCAGGGCGAGGTGGGCTGGGTTTCCCCCGAAGTCATGCAGTTCGTCGCCAGCTATC
TCGACATGCCGCCCGTGTGGGTGGAAGAGGTGGCGACCTTCTACAACATGTACGACACCAAGCCGGTGGGTAGATTCAAG
CTCGCCGTCTGCACCAACCTGCCCTGCGCCCTGTCGGGCGGCGAGCGGGCCGGCGAGTACCTCAAGCGCAAGCTTGGCAT
CGACTACAACGAGACCACTGCCGACGGCTGCTTCACCTTGAAAGAAGGTGAGTGCATGGGCGCTTGCGGCGACGCGCCGG
TAATGATCGTCAACAACACCCGCATGTGCAGCTTCATGAGCGACGACAAGCTCGACGCGCTGGTCGACGAGCTGAAGGCC
GACGCTGCCGCCAAGGGGGCAAAGTAA

Upstream 100 bases:

>100_bases
CGGCGGAACGGCAGGGCCTAAGGCCCGCCGGCGGAACAGGCAGCGACGGCTGCAAACCTGCGGATGCAAGCCCGTTTGCT
GCCTCCGCCGTTTTACTGCA

Downstream 100 bases:

>100_bases
CATGACCTCTCTGCACGACCGCCACATCCAGCCGCTGATCCTGGCCGGCCTGGACGGCAAGAACTGGCACCTGGAAGACT
ACGTCAAGCGCGGCGGCTAC

Product: NADH dehydrogenase subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E 2; NDH-1 subunit E 2 [H]

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MTMLSAEALKEIDRAIAKYPADQKQSAVMAALAVAQGEVGWVSPEVMQFVASYLDMPPVWVEEVATFYNMYDTKPVGRFK
LAVCTNLPCALSGGERAGEYLKRKLGIDYNETTADGCFTLKEGECMGACGDAPVMIVNNTRMCSFMSDDKLDALVDELKA
DAAAKGAK

Sequences:

>Translated_168_residues
MTMLSAEALKEIDRAIAKYPADQKQSAVMAALAVAQGEVGWVSPEVMQFVASYLDMPPVWVEEVATFYNMYDTKPVGRFK
LAVCTNLPCALSGGERAGEYLKRKLGIDYNETTADGCFTLKEGECMGACGDAPVMIVNNTRMCSFMSDDKLDALVDELKA
DAAAKGAK
>Mature_167_residues
TMLSAEALKEIDRAIAKYPADQKQSAVMAALAVAQGEVGWVSPEVMQFVASYLDMPPVWVEEVATFYNMYDTKPVGRFKL
AVCTNLPCALSGGERAGEYLKRKLGIDYNETTADGCFTLKEGECMGACGDAPVMIVNNTRMCSFMSDDKLDALVDELKAD
AAAKGAK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=162, Percent_Identity=40.7407407407407, Blast_Score=132, Evalue=2e-31,
Organism=Escherichia coli, GI1788621, Length=135, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17561328, Length=157, Percent_Identity=38.8535031847134, Blast_Score=120, Evalue=3e-28,
Organism=Drosophila melanogaster, GI18859877, Length=158, Percent_Identity=39.873417721519, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21355353, Length=158, Percent_Identity=35.4430379746835, Blast_Score=107, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 18169; Mature: 18038

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
9.5 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
9.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMLSAEALKEIDRAIAKYPADQKQSAVMAALAVAQGEVGWVSPEVMQFVASYLDMPPVW
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHH
VEEVATFYNMYDTKPVGRFKLAVCTNLPCALSGGERAGEYLKRKLGIDYNETTADGCFTL
HHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEE
KEGECMGACGDAPVMIVNNTRMCSFMSDDKLDALVDELKADAAAKGAK
CCCCCCCCCCCCCEEEEECCEEEECCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TMLSAEALKEIDRAIAKYPADQKQSAVMAALAVAQGEVGWVSPEVMQFVASYLDMPPVW
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHH
VEEVATFYNMYDTKPVGRFKLAVCTNLPCALSGGERAGEYLKRKLGIDYNETTADGCFTL
HHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEE
KEGECMGACGDAPVMIVNNTRMCSFMSDDKLDALVDELKADAAAKGAK
CCCCCCCCCCCCCEEEEECCEEEECCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]