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The map label for this gene is nuoB [H]

Identifier: 73540665

GI number: 73540665

Start: 1049726

End: 1050208

Strand: Direct

Name: nuoB [H]

Synonym: Reut_A0962

Alternate gene names: 73540665

Gene position: 1049726-1050208 (Clockwise)

Preceding gene: 73540664

Following gene: 73540666

Centisome position: 27.58

GC content: 62.94

Gene sequence:

>483_bases
ATGGCAATCGAAGGCGTTCTCAACGAAGGCTTTGTCACGACTACCGCTGACAAGCTGATCAACTGGACCCGCACCGGGTC
GCTGTGGCCGATGACCTTCGGCCTGGCCTGCTGTGCTGTGGAAATGATGCATGCTGGCGCGGCGCGCTATGACCTGGACC
GCTTCGGCGTGGTGTTCCGCCCGTCGCCGCGCCAGTCGGACGTGATGATCGTGGCCGGCACGCTTTGCAACAAGATGGCG
CCCGCACTGCGCAAGGTCTACGACCAGATGGCCGAGCCACGCTGGGTGATCTCGATGGGCTCTTGCGCCAATGGCGGCGG
CTATTACCACTACTCGTATTCGGTGGTGCGTGGCTGCGACCGCATTGTGCCGGTGGACATCTACGTGCCGGGCTGCCCGC
CCACGGCTGAAGCGCTGATCTATGGCGTGATCCAGCTGCAGAACAAGATCAAGCGCACCAACACCATCGCGCGCAAGGGC
TGA

Upstream 100 bases:

>100_bases
CTGGCCGGGATTCATCGCCATGGGCGTGTTTCTGCTGGAATTCATCGTGGGCTTCGTCTACATCTGGAAAAAGGGCGCGC
TCGATTGGGAGTGATGTGAA

Downstream 100 bases:

>100_bases
CATGGCGAAGCTTGAGACCCTGAAGGCCGCGCTCGAGAAAGTTCTCGGCAAGCGCGTGCAGAAACTGATCGAGGCGACCG
GCGAACTGACGCTGATCGTC

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MAIEGVLNEGFVTTTADKLINWTRTGSLWPMTFGLACCAVEMMHAGAARYDLDRFGVVFRPSPRQSDVMIVAGTLCNKMA
PALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALIYGVIQLQNKIKRTNTIARKG

Sequences:

>Translated_160_residues
MAIEGVLNEGFVTTTADKLINWTRTGSLWPMTFGLACCAVEMMHAGAARYDLDRFGVVFRPSPRQSDVMIVAGTLCNKMA
PALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALIYGVIQLQNKIKRTNTIARKG
>Mature_159_residues
AIEGVLNEGFVTTTADKLINWTRTGSLWPMTFGLACCAVEMMHAGAARYDLDRFGVVFRPSPRQSDVMIVAGTLCNKMAP
ALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALIYGVIQLQNKIKRTNTIARKG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=146, Percent_Identity=80.8219178082192, Blast_Score=261, Evalue=3e-70,
Organism=Escherichia coli, GI1788624, Length=151, Percent_Identity=49.6688741721854, Blast_Score=165, Evalue=1e-42,
Organism=Escherichia coli, GI1789074, Length=139, Percent_Identity=36.6906474820144, Blast_Score=98, Evalue=3e-22,
Organism=Escherichia coli, GI1788834, Length=123, Percent_Identity=39.0243902439024, Blast_Score=89, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17509685, Length=136, Percent_Identity=75.7352941176471, Blast_Score=238, Evalue=7e-64,
Organism=Drosophila melanogaster, GI18859983, Length=140, Percent_Identity=82.8571428571429, Blast_Score=261, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24642371, Length=140, Percent_Identity=82.8571428571429, Blast_Score=261, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24651058, Length=140, Percent_Identity=82.1428571428571, Blast_Score=255, Evalue=9e-69,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 17603; Mature: 17472

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
8.8 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
8.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIEGVLNEGFVTTTADKLINWTRTGSLWPMTFGLACCAVEMMHAGAARYDLDRFGVVFR
CCCCCCCCCCCEEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEC
PSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCD
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEHHHHHCCC
RIVPVDIYVPGCPPTAEALIYGVIQLQNKIKRTNTIARKG
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIEGVLNEGFVTTTADKLINWTRTGSLWPMTFGLACCAVEMMHAGAARYDLDRFGVVFR
CCCCCCCCCCEEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEC
PSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCD
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEHHHHHCCC
RIVPVDIYVPGCPPTAEALIYGVIQLQNKIKRTNTIARKG
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA