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The map label for this gene is ksgA [H]

Identifier: 73540206

GI number: 73540206

Start: 544306

End: 545142

Strand: Direct

Name: ksgA [H]

Synonym: Reut_A0500

Alternate gene names: 73540206

Gene position: 544306-545142 (Clockwise)

Preceding gene: 73540205

Following gene: 73540207

Centisome position: 14.3

GC content: 65.71

Gene sequence:

>837_bases
ATGCGTTCAAACATGCACCAGGGCCACGTGGCCCGCAAGCGATTCGGCCAGAACTTCCTGGTCGACGATTCCATCATTCA
CGGCATCGTCAATGCCATCAATCCGTTGGCCGACGATGTGCTAGTGGAGATCGGCCCGGGCCTCGGCGCGCTGACCGATC
CGCTGCTCGAGCGCGTGCCGCAGATGCAGGTGGTCGAGCTGGACCGCGATCTGGTCGAGCGCCTGCGGCGCCGCTATGGC
GACCGCCTGCAGGTCCATGCCGGCGACGCGCTGGCCTTCGACTTCGGCAGGCTCGCGGTGCCCGGGCGGCCATTGCGCAT
CGTCGGCAACCTGCCGTACAACATTTCCAGCCCGCTGCTGTTCCACCTGATGGACTACGCCGACCACGTGCACGACCAGC
ACTTCATGCTGCAAAAGGAAGTGGTCGACCGCATGGTGGCTGAACCAGGCAGCAAGGCCTTCGGCCGACTGTCGATCATG
CTCCAGGTGCGCTACCACATGGAGCATGTGCTCGACGTGCCGCCGGGTTCGTTCAATCCGCCGCCGAAGGTCGATTCCGC
CGTGGTGCGCATGATCCCGTGGCCGCGCACGGAAAGCGGCCGGCTGCGCTCGCCGCATGCCGATTGCGACATTACCGTGC
TCGGCGATCTGGTGACCGCAGCGTTCTCGCAGCGGCGCAAGGTGTTGCGCAATACGCTCTCGTTCCTGCGCGACCAGATC
GATTTCGAGGCCATCGGCTTCGACCTTGGCCGCCGCGCTGAAGAGGTGCCGGTCGGCGAGTACGTCGAGCTGGCGCGCCG
CTTGGGCGGCGATGCATCCGACAGATCGGCCGCCTGA

Upstream 100 bases:

>100_bases
GCAGCATGATCGAGGCTATCCGCAGTGCGGTCACCATGGCCGGACATGCCAACGGCAGGCATGCTTCAGCCAGGGTGCCG
CACTAGTGGTGTATTCCTCC

Downstream 100 bases:

>100_bases
AGGCCGGACCGCTTACATCCCCAAATTTCCGCGAATTTCCGCAATGACAGCCAACACGCAAACCAACAAGCGACCCGTCA
TCCTGACCGGCGATCGTCCC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MRSNMHQGHVARKRFGQNFLVDDSIIHGIVNAINPLADDVLVEIGPGLGALTDPLLERVPQMQVVELDRDLVERLRRRYG
DRLQVHAGDALAFDFGRLAVPGRPLRIVGNLPYNISSPLLFHLMDYADHVHDQHFMLQKEVVDRMVAEPGSKAFGRLSIM
LQVRYHMEHVLDVPPGSFNPPPKVDSAVVRMIPWPRTESGRLRSPHADCDITVLGDLVTAAFSQRRKVLRNTLSFLRDQI
DFEAIGFDLGRRAEEVPVGEYVELARRLGGDASDRSAA

Sequences:

>Translated_278_residues
MRSNMHQGHVARKRFGQNFLVDDSIIHGIVNAINPLADDVLVEIGPGLGALTDPLLERVPQMQVVELDRDLVERLRRRYG
DRLQVHAGDALAFDFGRLAVPGRPLRIVGNLPYNISSPLLFHLMDYADHVHDQHFMLQKEVVDRMVAEPGSKAFGRLSIM
LQVRYHMEHVLDVPPGSFNPPPKVDSAVVRMIPWPRTESGRLRSPHADCDITVLGDLVTAAFSQRRKVLRNTLSFLRDQI
DFEAIGFDLGRRAEEVPVGEYVELARRLGGDASDRSAA
>Mature_278_residues
MRSNMHQGHVARKRFGQNFLVDDSIIHGIVNAINPLADDVLVEIGPGLGALTDPLLERVPQMQVVELDRDLVERLRRRYG
DRLQVHAGDALAFDFGRLAVPGRPLRIVGNLPYNISSPLLFHLMDYADHVHDQHFMLQKEVVDRMVAEPGSKAFGRLSIM
LQVRYHMEHVLDVPPGSFNPPPKVDSAVVRMIPWPRTESGRLRSPHADCDITVLGDLVTAAFSQRRKVLRNTLSFLRDQI
DFEAIGFDLGRRAEEVPVGEYVELARRLGGDASDRSAA

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657198, Length=219, Percent_Identity=34.2465753424658, Blast_Score=114, Evalue=8e-26,
Organism=Homo sapiens, GI156415992, Length=247, Percent_Identity=28.7449392712551, Blast_Score=92, Evalue=4e-19,
Organism=Escherichia coli, GI1786236, Length=275, Percent_Identity=51.2727272727273, Blast_Score=275, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI25146882, Length=183, Percent_Identity=35.5191256830601, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25141369, Length=290, Percent_Identity=25.8620689655172, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324989, Length=188, Percent_Identity=31.9148936170213, Blast_Score=99, Evalue=8e-22,
Organism=Drosophila melanogaster, GI21358017, Length=234, Percent_Identity=33.7606837606838, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI21357273, Length=272, Percent_Identity=30.5147058823529, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 31189; Mature: 31189

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSNMHQGHVARKRFGQNFLVDDSIIHGIVNAINPLADDVLVEIGPGLGALTDPLLERVP
CCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
QMQVVELDRDLVERLRRRYGDRLQVHAGDALAFDFGRLAVPGRPLRIVGNLPYNISSPLL
CHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEECCCCCCCCCCCEEEEECCCCCCCHHHH
FHLMDYADHVHDQHFMLQKEVVDRMVAEPGSKAFGRLSIMLQVRYHMEHVLDVPPGSFNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PPKVDSAVVRMIPWPRTESGRLRSPHADCDITVLGDLVTAAFSQRRKVLRNTLSFLRDQI
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFEAIGFDLGRRAEEVPVGEYVELARRLGGDASDRSAA
HHHHHCHHHHCCHHCCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRSNMHQGHVARKRFGQNFLVDDSIIHGIVNAINPLADDVLVEIGPGLGALTDPLLERVP
CCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
QMQVVELDRDLVERLRRRYGDRLQVHAGDALAFDFGRLAVPGRPLRIVGNLPYNISSPLL
CHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEECCCCCCCCCCCEEEEECCCCCCCHHHH
FHLMDYADHVHDQHFMLQKEVVDRMVAEPGSKAFGRLSIMLQVRYHMEHVLDVPPGSFNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PPKVDSAVVRMIPWPRTESGRLRSPHADCDITVLGDLVTAAFSQRRKVLRNTLSFLRDQI
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFEAIGFDLGRRAEEVPVGEYVELARRLGGDASDRSAA
HHHHHCHHHHCCHHCCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA