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The map label for this gene is ysgA [C]

Identifier: 73539591

GI number: 73539591

Start: 2596344

End: 2597090

Strand: Reverse

Name: ysgA [C]

Synonym: Reut_B5771

Alternate gene names: 73539591

Gene position: 2597090-2596344 (Counterclockwise)

Preceding gene: 73539592

Following gene: 73539590

Centisome position: 95.27

GC content: 66.4

Gene sequence:

>747_bases
ATGAAGGAAAGCATCGACGACAAGACGACGATCCGCACGAGCTGGATCGACATCCCCGCAGCCGATGGTCGCGCGTACCA
AGGCTATCTCGCGCTGCCGCCAGCGAGAACGGGCCCCGGGATTGTCCTCTTCCAGGAAATCTTCGGCGTGAACCGCCATA
TCCGGGCCGTAGCCGAGCAGTACGCGCAACTGGGCTTTGTCGTGCTCGCGCCCGACGTGTTCTGGCGGCAGGCGCCGCGC
GAATCGTTCGGCTATGTGGGCGATGAAGCCGCTCGCGCGCGTGACATGCTCATTCGCGCCGACATGGCAGAGATCGAGAG
CGACCTGGAAGGCACCGTGCGATCGCTGCGCGAGCGTCCCGAAGTGACGGGCGGCGTGGGGGCGCTCGGCTTCTGCATCG
GTGGCCGCCTGGCCTATCTGGCCGCCGCTGCGGGACTGGTGGACACCGCCGCGTGCTACTACGGCGGCGGCATTCAGGAC
CAGTTACAGAAGGCGTCGGCTGTGCGTTGCCCGATGCTGTTCCACTACGGCGAGACCGATCCCATGATCCCGGTCGCCGC
AGTCGAGCGGGTCAAGGCAGCCTTTGCCGACCGCGACACGGCGAAATTCTTCGTCTATCCCGGCGCAAGCCACGGCTTCA
ACTGCTGGGAGCGTGCCAGTTATCAGCCGCTCGCCGCGATGCAGGCGCACGGTCGGACGGTGCTGCACTTCGCCCGCCAT
CTTGCCGCAGCCCCCACGGTCGCATAG

Upstream 100 bases:

>100_bases
TTGCGCGAGAAGTGAACGAGGCTGATCAGTGCCGCCGACTCCGTGCAGGCGAGGCCACGCTCGACATCTTTGGCTGGCGC
TGAGGAAGGAGGCAGACGAC

Downstream 100 bases:

>100_bases
CCTCCGGACGGAGAACTCGCATGAGCATCGACGCCAACCAGTTCAGGACAGCGATGCGCTGCCTCGCCGGACATGTCTGC
CTGATTACCACGTTGTCGGC

Product: carboxymethylenebutenolidase

Products: NA

Alternate protein names: Dienelactone hydrolase; DLH [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MKESIDDKTTIRTSWIDIPAADGRAYQGYLALPPARTGPGIVLFQEIFGVNRHIRAVAEQYAQLGFVVLAPDVFWRQAPR
ESFGYVGDEAARARDMLIRADMAEIESDLEGTVRSLRERPEVTGGVGALGFCIGGRLAYLAAAAGLVDTAACYYGGGIQD
QLQKASAVRCPMLFHYGETDPMIPVAAVERVKAAFADRDTAKFFVYPGASHGFNCWERASYQPLAAMQAHGRTVLHFARH
LAAAPTVA

Sequences:

>Translated_248_residues
MKESIDDKTTIRTSWIDIPAADGRAYQGYLALPPARTGPGIVLFQEIFGVNRHIRAVAEQYAQLGFVVLAPDVFWRQAPR
ESFGYVGDEAARARDMLIRADMAEIESDLEGTVRSLRERPEVTGGVGALGFCIGGRLAYLAAAAGLVDTAACYYGGGIQD
QLQKASAVRCPMLFHYGETDPMIPVAAVERVKAAFADRDTAKFFVYPGASHGFNCWERASYQPLAAMQAHGRTVLHFARH
LAAAPTVA
>Mature_248_residues
MKESIDDKTTIRTSWIDIPAADGRAYQGYLALPPARTGPGIVLFQEIFGVNRHIRAVAEQYAQLGFVVLAPDVFWRQAPR
ESFGYVGDEAARARDMLIRADMAEIESDLEGTVRSLRERPEVTGGVGALGFCIGGRLAYLAAAAGLVDTAACYYGGGIQD
QLQKASAVRCPMLFHYGETDPMIPVAAVERVKAAFADRDTAKFFVYPGASHGFNCWERASYQPLAAMQAHGRTVLHFARH
LAAAPTVA

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dienelactone hydrolase family [H]

Homologues:

Organism=Escherichia coli, GI48994982, Length=242, Percent_Identity=26.8595041322314, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: =3.1.1.45 [H]

Molecular weight: Translated: 26925; Mature: 26925

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKESIDDKTTIRTSWIDIPAADGRAYQGYLALPPARTGPGIVLFQEIFGVNRHIRAVAEQ
CCCCCCCCCEEEEEEEECCCCCCCCCCCEEECCCCCCCCCEEHHHHHHCCCHHHHHHHHH
YAQLGFVVLAPDVFWRQAPRESFGYVGDEAARARDMLIRADMAEIESDLEGTVRSLRERP
HHHCCEEEECCHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EVTGGVGALGFCIGGRLAYLAAAAGLVDTAACYYGGGIQDQLQKASAVRCPMLFHYGETD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCCC
PMIPVAAVERVKAAFADRDTAKFFVYPGASHGFNCWERASYQPLAAMQAHGRTVLHFARH
CCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHCCCCCHHHHHHCCHHHHHHHHH
LAAAPTVA
HHCCCCCC
>Mature Secondary Structure
MKESIDDKTTIRTSWIDIPAADGRAYQGYLALPPARTGPGIVLFQEIFGVNRHIRAVAEQ
CCCCCCCCCEEEEEEEECCCCCCCCCCCEEECCCCCCCCCEEHHHHHHCCCHHHHHHHHH
YAQLGFVVLAPDVFWRQAPRESFGYVGDEAARARDMLIRADMAEIESDLEGTVRSLRERP
HHHCCEEEECCHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EVTGGVGALGFCIGGRLAYLAAAAGLVDTAACYYGGGIQDQLQKASAVRCPMLFHYGETD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCCC
PMIPVAAVERVKAAFADRDTAKFFVYPGASHGFNCWERASYQPLAAMQAHGRTVLHFARH
CCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHCCCCCHHHHHHCCHHHHHHHHH
LAAAPTVA
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]