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The map label for this gene is rutF2 [H]

Identifier: 73539590

GI number: 73539590

Start: 2595772

End: 2596323

Strand: Reverse

Name: rutF2 [H]

Synonym: Reut_B5770

Alternate gene names: 73539590

Gene position: 2596323-2595772 (Counterclockwise)

Preceding gene: 73539591

Following gene: 73539589

Centisome position: 95.24

GC content: 65.94

Gene sequence:

>552_bases
ATGAGCATCGACGCCAACCAGTTCAGGACAGCGATGCGCTGCCTCGCCGGACATGTCTGCCTGATTACCACGTTGTCGGC
GGACGGCTCGCGTGCGGGGCTAACAGCCACGGCCGTCTGCTCGGTCTCGGCCGATCCTCCCACGCTGCTCGCCTGTGTCA
ACCGCAACAGTGCGTCGTTCCACGCGATCCAGGCATCAGGGATCTTTGCGGTGAACGTGCTGGCGGTGGAAAGCCGCCGG
CTGGCGGACCGCTTTGCCAGCCCCATACCGGCAGAGGAAAAGTTTGCCGACGGCGTATGGGGACGCGCCAGCACCGGCGC
GCCAGTTTTCCGCGACGCGTTGGTCAGCTTCGATTGCCGAGTGGCCAGTGCGTTGGACGTTGGCACGCACGGCATTCTCT
TCGGCGCCATCGACACCGCAACGCCCCGCGCCGACCGCGCCCGGCCGCTGCTGTACGCGCACGGCAGCTACGGCGAATTT
TCATCGACCGACGCTGCCGCAGCTCCAGAACTGCTCTGGATTCCGACATGGGACCCGGAGCCTGCTAGCTGA

Upstream 100 bases:

>100_bases
CGCTCGCCGCGATGCAGGCGCACGGTCGGACGGTGCTGCACTTCGCCCGCCATCTTGCCGCAGCCCCCACGGTCGCATAG
CCTCCGGACGGAGAACTCGC

Downstream 100 bases:

>100_bases
GGCGGACCCTCGCAAATCTGCCGGTCGACCGAAACCGGCCTGCTCCCCTGCAGGGCAGGCACCACAACAATAAATTCCAA
CCACGGAGACACGGAATGGC

Product: flavin reductase-like protein

Products: NA

Alternate protein names: FMN reductase 2; NAD(P)H:flavin oxidoreductase 2 [H]

Number of amino acids: Translated: 183; Mature: 182

Protein sequence:

>183_residues
MSIDANQFRTAMRCLAGHVCLITTLSADGSRAGLTATAVCSVSADPPTLLACVNRNSASFHAIQASGIFAVNVLAVESRR
LADRFASPIPAEEKFADGVWGRASTGAPVFRDALVSFDCRVASALDVGTHGILFGAIDTATPRADRARPLLYAHGSYGEF
SSTDAAAAPELLWIPTWDPEPAS

Sequences:

>Translated_183_residues
MSIDANQFRTAMRCLAGHVCLITTLSADGSRAGLTATAVCSVSADPPTLLACVNRNSASFHAIQASGIFAVNVLAVESRR
LADRFASPIPAEEKFADGVWGRASTGAPVFRDALVSFDCRVASALDVGTHGILFGAIDTATPRADRARPLLYAHGSYGEF
SSTDAAAAPELLWIPTWDPEPAS
>Mature_182_residues
SIDANQFRTAMRCLAGHVCLITTLSADGSRAGLTATAVCSVSADPPTLLACVNRNSASFHAIQASGIFAVNVLAVESRRL
ADRFASPIPAEEKFADGVWGRASTGAPVFRDALVSFDCRVASALDVGTHGILFGAIDTATPRADRARPLLYAHGSYGEFS
STDAAAAPELLWIPTWDPEPAS

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=136, Percent_Identity=44.1176470588235, Blast_Score=115, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 19166; Mature: 19034

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDANQFRTAMRCLAGHVCLITTLSADGSRAGLTATAVCSVSADPPTLLACVNRNSASF
CCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCE
HAIQASGIFAVNVLAVESRRLADRFASPIPAEEKFADGVWGRASTGAPVFRDALVSFDCR
EEEEECCEEEEEEEEECHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCH
VASALDVGTHGILFGAIDTATPRADRARPLLYAHGSYGEFSSTDAAAAPELLWIPTWDPE
HHHHHCCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCCC
PAS
CCC
>Mature Secondary Structure 
SIDANQFRTAMRCLAGHVCLITTLSADGSRAGLTATAVCSVSADPPTLLACVNRNSASF
CCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCE
HAIQASGIFAVNVLAVESRRLADRFASPIPAEEKFADGVWGRASTGAPVFRDALVSFDCR
EEEEECCEEEEEEEEECHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCH
VASALDVGTHGILFGAIDTATPRADRARPLLYAHGSYGEFSSTDAAAAPELLWIPTWDPE
HHHHHCCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCCC
PAS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA