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The map label for this gene is pdhC [H]

Identifier: 73538803

GI number: 73538803

Start: 1705155

End: 1706264

Strand: Direct

Name: pdhC [H]

Synonym: Reut_B4978

Alternate gene names: 73538803

Gene position: 1705155-1706264 (Clockwise)

Preceding gene: 73538802

Following gene: 73538805

Centisome position: 62.55

GC content: 69.19

Gene sequence:

>1110_bases
ATGAGAGTCTTCAAGCTGCCCGACCTGGGCGAAGGCCTGCAGGAAGCCGAGATCGTGACGTGGCATGTGAAGGTCGGCGA
CACCGTGGCCGCGGATCAGCCGTTGCTGTCGGTCGAAACGGCCAAGGCCATTGTGGAAATCCCCTCGCCGTATGCCGGCA
CGATTGGCAAGCTGTTTGCACAGGCGGGCGACCTCGTCCACCTTGGCGCGCCGCTCGCGAGCTTCGAAGGCGCGGGCAAC
GATGCCGATGCGGGCACCGTAGTAGGCGCGGTCAAGGTCGGCTCGCATGTGGTGGCAGAATCCGCCACGCCGCTGAGCGG
GGGTGCGGCCGGCGCGGGCATCAAGGCCACGCCGGCGGTGCGCGCGCTGGCAAGGCGGCTCGGCGTGGACCTGGCGATGG
CAAACCCGTCCGGGCCCGACGGTGTCGTCACGGCTGCTGATGTGGAACGCGTCGCATCAACACTGGCTGAAACCGGCCCG
GGTGAAGTGCTGCGCGGCGTGCGTCGTGCGATGGCGCAGAACATGGCGCGCGCGCAGAGCGAAGTCGCCGCCGCGACCGT
GATGGACGATGCCGATATCCATGCGTGGCAAGGCGCGCATGACGTGACGATCCGGCTGGTGCGCGCACTGGTGGCCGGTT
GCCGTGCGGAGCCGGGGCTCAATGGCTGGTACGAGGGCCAGACCGGCAAGCGGCACGTGATGCAGAAGATCGACGTCGGC
ATCGCCGCCGACCTGCCCGAAGGCCTGTTCGTTCCCGTGCTGCGTAATGTGGGCAACCGCGACGCCGCGGATCTGCGACA
CGGCCTGGACCGCATGCGCGCCGATATCCGCGCGCGCACGATCGCGCCCGAGGAAATGCGCGGCAACACCATCACGCTGT
CCAACTTCGGCATGATCGCGGGCCGCTATGCCGCGCCGATCGTGGTGCCGCCCACGGTGGCGATCCTCGGCGCCGGCCAT
ATCCGCGACGAAGTCGTCGCGGCCAATGGCGTGCCGGCCGTGCACCGCGTGATCCCGCTGAGCCTGACCTTCGACCACCG
CGTGGTCACGGGTGGCGAGGCCGCGCGTTTCCTGGCCGCGGTGATCGCCGACCTGGCGCTGCCCGAATAG

Upstream 100 bases:

>100_bases
GCGGCTGGAACACACCTATCTGCCGAGCGTGGCGCGCATTGTCGATGCCGTGCGCAAGGCGCTGTCGGCATAGTGGCAAT
AGTCTTACGGGGACGCAACG

Downstream 100 bases:

>100_bases
GGACAGGCGAATTCAGGCCAGCCGGATCTCGCCGTCGAACGTCGCGCTGAGCTTGAGCTTGCCGAGCTCGAGCGTCATCT
TGACCGGCAGGATCTCGTTC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2; S complex, 48 kDa subunit [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MRVFKLPDLGEGLQEAEIVTWHVKVGDTVAADQPLLSVETAKAIVEIPSPYAGTIGKLFAQAGDLVHLGAPLASFEGAGN
DADAGTVVGAVKVGSHVVAESATPLSGGAAGAGIKATPAVRALARRLGVDLAMANPSGPDGVVTAADVERVASTLAETGP
GEVLRGVRRAMAQNMARAQSEVAAATVMDDADIHAWQGAHDVTIRLVRALVAGCRAEPGLNGWYEGQTGKRHVMQKIDVG
IAADLPEGLFVPVLRNVGNRDAADLRHGLDRMRADIRARTIAPEEMRGNTITLSNFGMIAGRYAAPIVVPPTVAILGAGH
IRDEVVAANGVPAVHRVIPLSLTFDHRVVTGGEAARFLAAVIADLALPE

Sequences:

>Translated_369_residues
MRVFKLPDLGEGLQEAEIVTWHVKVGDTVAADQPLLSVETAKAIVEIPSPYAGTIGKLFAQAGDLVHLGAPLASFEGAGN
DADAGTVVGAVKVGSHVVAESATPLSGGAAGAGIKATPAVRALARRLGVDLAMANPSGPDGVVTAADVERVASTLAETGP
GEVLRGVRRAMAQNMARAQSEVAAATVMDDADIHAWQGAHDVTIRLVRALVAGCRAEPGLNGWYEGQTGKRHVMQKIDVG
IAADLPEGLFVPVLRNVGNRDAADLRHGLDRMRADIRARTIAPEEMRGNTITLSNFGMIAGRYAAPIVVPPTVAILGAGH
IRDEVVAANGVPAVHRVIPLSLTFDHRVVTGGEAARFLAAVIADLALPE
>Mature_369_residues
MRVFKLPDLGEGLQEAEIVTWHVKVGDTVAADQPLLSVETAKAIVEIPSPYAGTIGKLFAQAGDLVHLGAPLASFEGAGN
DADAGTVVGAVKVGSHVVAESATPLSGGAAGAGIKATPAVRALARRLGVDLAMANPSGPDGVVTAADVERVASTLAETGP
GEVLRGVRRAMAQNMARAQSEVAAATVMDDADIHAWQGAHDVTIRLVRALVAGCRAEPGLNGWYEGQTGKRHVMQKIDVG
IAADLPEGLFVPVLRNVGNRDAADLRHGLDRMRADIRARTIAPEEMRGNTITLSNFGMIAGRYAAPIVVPPTVAILGAGH
IRDEVVAANGVPAVHRVIPLSLTFDHRVVTGGEAARFLAAVIADLALPE

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity [H]

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=387, Percent_Identity=28.4237726098191, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI110671329, Length=407, Percent_Identity=27.2727272727273, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI31711992, Length=429, Percent_Identity=24.4755244755245, Blast_Score=95, Evalue=9e-20,
Organism=Homo sapiens, GI203098753, Length=440, Percent_Identity=20.6818181818182, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI203098816, Length=440, Percent_Identity=20.6818181818182, Blast_Score=82, Evalue=9e-16,
Organism=Escherichia coli, GI1786305, Length=412, Percent_Identity=29.8543689320388, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1786946, Length=397, Percent_Identity=24.9370277078086, Blast_Score=117, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17537937, Length=406, Percent_Identity=26.8472906403941, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI25146366, Length=392, Percent_Identity=29.0816326530612, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17560088, Length=424, Percent_Identity=23.3490566037736, Blast_Score=78, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17538894, Length=167, Percent_Identity=26.9461077844311, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6320352, Length=392, Percent_Identity=26.7857142857143, Blast_Score=116, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6324258, Length=158, Percent_Identity=27.2151898734177, Blast_Score=73, Evalue=8e-14,
Organism=Drosophila melanogaster, GI18859875, Length=418, Percent_Identity=25.5980861244019, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24582497, Length=126, Percent_Identity=30.952380952381, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20129315, Length=126, Percent_Identity=30.952380952381, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 38151; Mature: 38151

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVFKLPDLGEGLQEAEIVTWHVKVGDTVAADQPLLSVETAKAIVEIPSPYAGTIGKLFA
CCEECCCCCCCCCCCCEEEEEEEEECCEECCCCCCHHHHHHHHHEECCCCCCHHHHHHHH
QAGDLVHLGAPLASFEGAGNDADAGTVVGAVKVGSHVVAESATPLSGGAAGAGIKATPAV
HCCCEEEECCCHHHCCCCCCCCCCCCEEEHHHHCCCHHHCCCCCCCCCCCCCCCCCCHHH
RALARRLGVDLAMANPSGPDGVVTAADVERVASTLAETGPGEVLRGVRRAMAQNMARAQS
HHHHHHHCCEEEECCCCCCCCCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
EVAAATVMDDADIHAWQGAHDVTIRLVRALVAGCRAEPGLNGWYEGQTGKRHVMQKIDVG
HHHHHHEECCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCC
IAADLPEGLFVPVLRNVGNRDAADLRHGLDRMRADIRARTIAPEEMRGNTITLSNFGMIA
EECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEECCCCCH
GRYAAPIVVPPTVAILGAGHIRDEVVAANGVPAVHRVIPLSLTFDHRVVTGGEAARFLAA
HCCCCCEEECCCEEEEECCCHHHHHEECCCCCHHHHEEEEEEEECCEEEECCHHHHHHHH
VIADLALPE
HHHHHCCCC
>Mature Secondary Structure
MRVFKLPDLGEGLQEAEIVTWHVKVGDTVAADQPLLSVETAKAIVEIPSPYAGTIGKLFA
CCEECCCCCCCCCCCCEEEEEEEEECCEECCCCCCHHHHHHHHHEECCCCCCHHHHHHHH
QAGDLVHLGAPLASFEGAGNDADAGTVVGAVKVGSHVVAESATPLSGGAAGAGIKATPAV
HCCCEEEECCCHHHCCCCCCCCCCCCEEEHHHHCCCHHHCCCCCCCCCCCCCCCCCCHHH
RALARRLGVDLAMANPSGPDGVVTAADVERVASTLAETGPGEVLRGVRRAMAQNMARAQS
HHHHHHHCCEEEECCCCCCCCCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
EVAAATVMDDADIHAWQGAHDVTIRLVRALVAGCRAEPGLNGWYEGQTGKRHVMQKIDVG
HHHHHHEECCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCC
IAADLPEGLFVPVLRNVGNRDAADLRHGLDRMRADIRARTIAPEEMRGNTITLSNFGMIA
EECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEECCCCCH
GRYAAPIVVPPTVAILGAGHIRDEVVAANGVPAVHRVIPLSLTFDHRVVTGGEAARFLAA
HCCCCCEEECCCEEEEECCCHHHHHEECCCCCHHHHEEEEEEEECCEEEECCHHHHHHHH
VIADLALPE
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377 [H]