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The map label for this gene is tktB [C]

Identifier: 73538602

GI number: 73538602

Start: 1477330

End: 1478175

Strand: Direct

Name: tktB [C]

Synonym: Reut_B4777

Alternate gene names: 73538602

Gene position: 1477330-1478175 (Clockwise)

Preceding gene: 73538601

Following gene: 73538603

Centisome position: 54.19

GC content: 64.66

Gene sequence:

>846_bases
ATGCATTACGCAACCAACGAGGTGGCCGTGCCCCTCGCCGAACGCGCCTACCGCATCCGGCGCAACGCCCTGCTGATGGG
CGAAGTCCAGGGACAAGGCTACATTGGGCAGGCGCTCGATATCGCCGATGTCCTGGCCGTCGCCTACTTCGGCGCCATGC
GCTACCGCGCGGAAGAGCCGGACTGGGAAGGGCGCGATCGCTTCCTGCTGTCCAACGGGCACTATGCGATCGCTTTGTAC
GCCGCGCTGCTCGAAGCCGGCATCCTGCCGATGGAGGAACTCGAAACGTATGGCAGCGACGACAGCCGCCTGCCCATGTC
CGGCATGGCCAGCTACACGCCGGGCATGGAGATGTCCGGCGGCTCGCTGGGCCAGGGCCTGACCATTGCCGTGGGCCGCT
GCCTCGGCCTCAAGCGCAAGGGATCGGATGCGTTCGTCTACACGCTGTTCTCCGACGGCGAACTCGATGAAGGCGCGATC
TGGGAAGGCATCCAGTCGGCAAGCCACTGGAAGCTCGACAACCTGATCGGCATCGTCGACGTCAACAACCAGCAGGCCGA
TGGCCCGTCCAGCCAGATCATGGCGTTCGAGCCGCTGGTCGAAAAGCTGCAGGCCTTCGGCTGGTTCACGCAGCGCGTCG
ACGGCAACGACATCGACGCGGTCGCGGCGGCCTTCGATACCGCACGCAAGCATCCGGGCGAACAACCGCGCATGATCGTC
TGCGACACGCGCATGGGTTGCGGCGTGCCGTTCCTCGAACAACGCGAAAAGAATCACTTCATCCGGGTCGATGCCCACGA
ATGGCAACTCGCCCTGCAGGCTCTCGAAGCCGGGAGACAAGCATGA

Upstream 100 bases:

>100_bases
CTGCTCGTCTTCCTCGTGCCGGCCAGATCGGTCAACCGCTGAGCCAGCCATGTACACAGGCTTTCCCTTCGCTCCGACTA
CCCAATCCACAGGAGACAAC

Downstream 100 bases:

>100_bases
GCAGCAACAATGGCAAGCCGAAGCTGAAGACCTCGGCAATGATCGCCTCGATCGCCGGTGAAGGACAGGCGACACGTTCG
GCCCCGTTCGGCCACGCGCT

Product: transketolase

Products: D-ribose 5-phosphate; D-xylulose 5-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MHYATNEVAVPLAERAYRIRRNALLMGEVQGQGYIGQALDIADVLAVAYFGAMRYRAEEPDWEGRDRFLLSNGHYAIALY
AALLEAGILPMEELETYGSDDSRLPMSGMASYTPGMEMSGGSLGQGLTIAVGRCLGLKRKGSDAFVYTLFSDGELDEGAI
WEGIQSASHWKLDNLIGIVDVNNQQADGPSSQIMAFEPLVEKLQAFGWFTQRVDGNDIDAVAAAFDTARKHPGEQPRMIV
CDTRMGCGVPFLEQREKNHFIRVDAHEWQLALQALEAGRQA

Sequences:

>Translated_281_residues
MHYATNEVAVPLAERAYRIRRNALLMGEVQGQGYIGQALDIADVLAVAYFGAMRYRAEEPDWEGRDRFLLSNGHYAIALY
AALLEAGILPMEELETYGSDDSRLPMSGMASYTPGMEMSGGSLGQGLTIAVGRCLGLKRKGSDAFVYTLFSDGELDEGAI
WEGIQSASHWKLDNLIGIVDVNNQQADGPSSQIMAFEPLVEKLQAFGWFTQRVDGNDIDAVAAAFDTARKHPGEQPRMIV
CDTRMGCGVPFLEQREKNHFIRVDAHEWQLALQALEAGRQA
>Mature_281_residues
MHYATNEVAVPLAERAYRIRRNALLMGEVQGQGYIGQALDIADVLAVAYFGAMRYRAEEPDWEGRDRFLLSNGHYAIALY
AALLEAGILPMEELETYGSDDSRLPMSGMASYTPGMEMSGGSLGQGLTIAVGRCLGLKRKGSDAFVYTLFSDGELDEGAI
WEGIQSASHWKLDNLIGIVDVNNQQADGPSSQIMAFEPLVEKLQAFGWFTQRVDGNDIDAVAAAFDTARKHPGEQPRMIV
CDTRMGCGVPFLEQREKNHFIRVDAHEWQLALQALEAGRQA

Specific function: Unknown

COG id: COG3959

COG function: function code G; Transketolase, N-terminal subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transketolase family [H]

Homologues:

Organism=Homo sapiens, GI133778974, Length=252, Percent_Identity=34.5238095238095, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI205277463, Length=252, Percent_Identity=34.5238095238095, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI4507521, Length=252, Percent_Identity=34.5238095238095, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI225637459, Length=251, Percent_Identity=28.2868525896414, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI225637461, Length=220, Percent_Identity=30, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI225637463, Length=218, Percent_Identity=30.2752293577982, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1788808, Length=267, Percent_Identity=32.5842696629214, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI48994911, Length=251, Percent_Identity=33.0677290836653, Blast_Score=110, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17539652, Length=252, Percent_Identity=33.3333333333333, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6325331, Length=231, Percent_Identity=35.4978354978355, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6319593, Length=230, Percent_Identity=31.304347826087, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI45551847, Length=252, Percent_Identity=31.7460317460317, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI45550715, Length=252, Percent_Identity=31.7460317460317, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24666278, Length=258, Percent_Identity=31.7829457364341, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24645119, Length=219, Percent_Identity=33.7899543378995, Blast_Score=111, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005474 [H]

Pfam domain/function: PF00456 Transketolase_N [H]

EC number: 2.2.1.1

Molecular weight: Translated: 30936; Mature: 30936

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYATNEVAVPLAERAYRIRRNALLMGEVQGQGYIGQALDIADVLAVAYFGAMRYRAEEP
CCCCCCCEECHHHHHHHHHHHCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
DWEGRDRFLLSNGHYAIALYAALLEAGILPMEELETYGSDDSRLPMSGMASYTPGMEMSG
CCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCHHCCCCCCCCC
GSLGQGLTIAVGRCLGLKRKGSDAFVYTLFSDGELDEGAIWEGIQSASHWKLDNLIGIVD
CCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHCCCCEECCCEEEEEE
VNNQQADGPSSQIMAFEPLVEKLQAFGWFTQRVDGNDIDAVAAAFDTARKHPGEQPRMIV
CCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEE
CDTRMGCGVPFLEQREKNHFIRVDAHEWQLALQALEAGRQA
EECCCCCCCCHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHYATNEVAVPLAERAYRIRRNALLMGEVQGQGYIGQALDIADVLAVAYFGAMRYRAEEP
CCCCCCCEECHHHHHHHHHHHCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
DWEGRDRFLLSNGHYAIALYAALLEAGILPMEELETYGSDDSRLPMSGMASYTPGMEMSG
CCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCHHCCCCCCCCC
GSLGQGLTIAVGRCLGLKRKGSDAFVYTLFSDGELDEGAIWEGIQSASHWKLDNLIGIVD
CCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHCCCCEECCCEEEEEE
VNNQQADGPSSQIMAFEPLVEKLQAFGWFTQRVDGNDIDAVAAAFDTARKHPGEQPRMIV
CCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEE
CDTRMGCGVPFLEQREKNHFIRVDAHEWQLALQALEAGRQA
EECCCCCCCCHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Thiamine diphosphate, mono-or triphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sedoheptulose 7-phosphate; D-glyceraldehyde 3-phosphate

Specific reaction: sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate

General reaction: Keto group transfer [C]

Inhibitor: EDTA; Oxythiamine diphosphate; Phosphate; Rabbit Antibodies; Sulfate [C]

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]