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The map label for this gene is fusA [H]

Identifier: 73537584

GI number: 73537584

Start: 343623

End: 345689

Strand: Reverse

Name: fusA [H]

Synonym: Reut_B3749

Alternate gene names: 73537584

Gene position: 345689-343623 (Counterclockwise)

Preceding gene: 73537585

Following gene: 73537583

Centisome position: 12.68

GC content: 65.55

Gene sequence:

>2067_bases
ATGCACTATAGTCCCGACGCAATTCGAACCATTGCCCTGCTGGGCCACGCGGGGTGCGGCAAGACCTCGCTAGCCGAGGC
GCTGCTGCACAAGGGGGGCGCCCTGCATGCCCCAGGCAGCGTCGAGCGGGGTTCAACGGTCAGCGACTTTGACCCGCTCG
AGCGCAAATACCACCACTCCCTCACCTCCGCAGTCCTGCACGTCGATTTCCGCAACACCCGCATCTACCTGATTGACACC
CCCGGATATCCCGACTTCTCTGGCCAGGCCATCAGCGCCCTGCCTGCGGTGGAAACGGCCGCCATCGTCATCAATGCCCA
GACCGGCATCGAGATGACCACGCGGCGCGCCATGGCGTGGGCCCAGGCGCGCAAGTTGTGCCGGATGATCATCGTCAACG
GCATCGATGGCGAGAAGGTCGACCTGCCAGCGCTGCTTGCCGACATCCAGGAAGCGTTCGGCAAGGAATGCCTGCCGATC
AACCTGCCGGCGGCGGGCGGCAGCCAGGTGGTGGACTGCTTTTTCAACCCTGCCGGCGAAGCGGATTTCCTGAGCGTGCC
CGCCGCGCATGATGCGCTGGTCGACCAGGTGATCGAAATCGATCCGGAGCTGATGGAGCTTTACCTCGAGCAAGGCGAAG
CGATTCAGCCCGAGCAGTTGCACGCGCCGTTCGAGCGCGCGCTGCGCGAAGGGCACCTGGTGCCGATCTGCTTCACCTCG
GCCGCCACCGGTGCGGGTGTGCCAGAACTGCTCGACGCGTTCGTGCGGCTGCTGCCGAACCCGACCGAAGGCAACCCGCC
CATGTTCTACCGCAGTGTCGGCGAAGGCAGCGACGCTGCGGAGCGCAAGAAGGAAGTGCGCGCCGAGCCCGTGCCCGACA
AGCATGTGCTCGCGCACGTCTTCAAGATCGTGATGGACCCGTACGTCGGCAAGATGGCGGTGTTCCGCATCCACCAGGGC
ACCGTCACGCGCGACAGCCAGCTCTACATCGGCGATGCGCGCCAGCCGATCAAGGTCGCGCATTTGCTGTTGCTGCAAGG
GAAGGAGCACGAGGAAGTGCCACGCGCGGGGCCAGGCGACATCTGTGCGGTGGCCAAGATCGACGAGATCGGCTTCGATG
CGGTGCTGCATGATGCCAACGAGGACGGCAATATCCACCTGATCCCGCTCGAATTCCCGACGCCGATCTATGGCCTCGCG
ATCGAGCCTGCACGCCGCGGCAACGAGCAGCGCATGGCCGAAGTCCTGCACAAGCTGTCCGCCGAAGATCCTTGCCTGCG
CGTCGAGCATCCGCCCGGCACGAATGAGACCGTGCTGCTCGGGCTCGGCGAGTTCCACTTGCGCTGCGTGATGGAGCGGC
TGACCGAGCAGTACAAGCTCGAAGTGGTCACACGGCCGCCGCGCATCGCCTATCGCGAAACCATTGGCGGATCGGCGGAG
GGCCATCACCGCCACAAGAAGCAGACCGGCGGCGCGGGTCAGTTTGGCGAGGTCATGCTGCGCGTGGACCCCCTGCCGCG
CGGCACGGGCTTCGAGTTCGTCGACGCGGTCAAGGGTGGCGCGATTCCCGGGCAGTTCATTCCGGCCGTGGAAAAAGGCA
TCCGCCAGGCACTGGAGTGCGGGCCGCTGGCCGGCTTCCCGATGCAGGACGTGCGCGTCACGGTGCTCGATGGCAAGAGC
CATCCTGTCGATTCCAAGGAAGTGGCATTCGCCACTGCCGGGCGCAAAGCCTTTGTCGACGCCGTGCTCAAGGCCAGGCC
CAGCGTGCTGGAACCGGTGGTCGATATCGAGGTGATGATGCCCGACTCGTCGATGGGCGACGTGATCGGCGATCTCTCGT
CCAAGCGCGGCCAGGTGCATGGCACCCGTACCGGTGCGGCCAACACGGTAATTGTCGCCGGCAAGGTCCCGCTGTCGGAG
CTTAACGACTACCAGTCGCGGCTGAACAGCCTGACCGGCGGCCACGGCAGCTACACCATCCAGTTCAGCCACTATGAGGG
CGTGCCGCCCGGGCAGCAGGACAAGATGGCGGCCAAGCACAAGGCGCAGGCCGATACCGGGGCCTGA

Upstream 100 bases:

>100_bases
CCTTTGTCTGCTCCCGCCCCATCGGCGGGTTTCCGCATATGGTGCTTCCGGCAATTTTCGCCAATAATGGCCTGACTGCC
ATTGCCGGACGAGAGCTGCC

Downstream 100 bases:

>100_bases
GCGCGTCCCCTCTGCGCGTATGGCCGCGGCACGCTCGCGGCCATACGCGAGCATCGCGCGCACCGTTCGGACGATAATGG
GCCCCGTTTCCCACTGATTG

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 688; Mature: 688

Protein sequence:

>688_residues
MHYSPDAIRTIALLGHAGCGKTSLAEALLHKGGALHAPGSVERGSTVSDFDPLERKYHHSLTSAVLHVDFRNTRIYLIDT
PGYPDFSGQAISALPAVETAAIVINAQTGIEMTTRRAMAWAQARKLCRMIIVNGIDGEKVDLPALLADIQEAFGKECLPI
NLPAAGGSQVVDCFFNPAGEADFLSVPAAHDALVDQVIEIDPELMELYLEQGEAIQPEQLHAPFERALREGHLVPICFTS
AATGAGVPELLDAFVRLLPNPTEGNPPMFYRSVGEGSDAAERKKEVRAEPVPDKHVLAHVFKIVMDPYVGKMAVFRIHQG
TVTRDSQLYIGDARQPIKVAHLLLLQGKEHEEVPRAGPGDICAVAKIDEIGFDAVLHDANEDGNIHLIPLEFPTPIYGLA
IEPARRGNEQRMAEVLHKLSAEDPCLRVEHPPGTNETVLLGLGEFHLRCVMERLTEQYKLEVVTRPPRIAYRETIGGSAE
GHHRHKKQTGGAGQFGEVMLRVDPLPRGTGFEFVDAVKGGAIPGQFIPAVEKGIRQALECGPLAGFPMQDVRVTVLDGKS
HPVDSKEVAFATAGRKAFVDAVLKARPSVLEPVVDIEVMMPDSSMGDVIGDLSSKRGQVHGTRTGAANTVIVAGKVPLSE
LNDYQSRLNSLTGGHGSYTIQFSHYEGVPPGQQDKMAAKHKAQADTGA

Sequences:

>Translated_688_residues
MHYSPDAIRTIALLGHAGCGKTSLAEALLHKGGALHAPGSVERGSTVSDFDPLERKYHHSLTSAVLHVDFRNTRIYLIDT
PGYPDFSGQAISALPAVETAAIVINAQTGIEMTTRRAMAWAQARKLCRMIIVNGIDGEKVDLPALLADIQEAFGKECLPI
NLPAAGGSQVVDCFFNPAGEADFLSVPAAHDALVDQVIEIDPELMELYLEQGEAIQPEQLHAPFERALREGHLVPICFTS
AATGAGVPELLDAFVRLLPNPTEGNPPMFYRSVGEGSDAAERKKEVRAEPVPDKHVLAHVFKIVMDPYVGKMAVFRIHQG
TVTRDSQLYIGDARQPIKVAHLLLLQGKEHEEVPRAGPGDICAVAKIDEIGFDAVLHDANEDGNIHLIPLEFPTPIYGLA
IEPARRGNEQRMAEVLHKLSAEDPCLRVEHPPGTNETVLLGLGEFHLRCVMERLTEQYKLEVVTRPPRIAYRETIGGSAE
GHHRHKKQTGGAGQFGEVMLRVDPLPRGTGFEFVDAVKGGAIPGQFIPAVEKGIRQALECGPLAGFPMQDVRVTVLDGKS
HPVDSKEVAFATAGRKAFVDAVLKARPSVLEPVVDIEVMMPDSSMGDVIGDLSSKRGQVHGTRTGAANTVIVAGKVPLSE
LNDYQSRLNSLTGGHGSYTIQFSHYEGVPPGQQDKMAAKHKAQADTGA
>Mature_688_residues
MHYSPDAIRTIALLGHAGCGKTSLAEALLHKGGALHAPGSVERGSTVSDFDPLERKYHHSLTSAVLHVDFRNTRIYLIDT
PGYPDFSGQAISALPAVETAAIVINAQTGIEMTTRRAMAWAQARKLCRMIIVNGIDGEKVDLPALLADIQEAFGKECLPI
NLPAAGGSQVVDCFFNPAGEADFLSVPAAHDALVDQVIEIDPELMELYLEQGEAIQPEQLHAPFERALREGHLVPICFTS
AATGAGVPELLDAFVRLLPNPTEGNPPMFYRSVGEGSDAAERKKEVRAEPVPDKHVLAHVFKIVMDPYVGKMAVFRIHQG
TVTRDSQLYIGDARQPIKVAHLLLLQGKEHEEVPRAGPGDICAVAKIDEIGFDAVLHDANEDGNIHLIPLEFPTPIYGLA
IEPARRGNEQRMAEVLHKLSAEDPCLRVEHPPGTNETVLLGLGEFHLRCVMERLTEQYKLEVVTRPPRIAYRETIGGSAE
GHHRHKKQTGGAGQFGEVMLRVDPLPRGTGFEFVDAVKGGAIPGQFIPAVEKGIRQALECGPLAGFPMQDVRVTVLDGKS
HPVDSKEVAFATAGRKAFVDAVLKARPSVLEPVVDIEVMMPDSSMGDVIGDLSSKRGQVHGTRTGAANTVIVAGKVPLSE
LNDYQSRLNSLTGGHGSYTIQFSHYEGVPPGQQDKMAAKHKAQADTGA

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=700, Percent_Identity=28.1428571428571, Blast_Score=288, Evalue=9e-78,
Organism=Homo sapiens, GI19923640, Length=727, Percent_Identity=27.0976616231087, Blast_Score=238, Evalue=2e-62,
Organism=Homo sapiens, GI25306287, Length=302, Percent_Identity=30.4635761589404, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI25306283, Length=449, Percent_Identity=25.3897550111359, Blast_Score=107, Evalue=5e-23,
Organism=Escherichia coli, GI1789738, Length=695, Percent_Identity=31.3669064748201, Blast_Score=341, Evalue=9e-95,
Organism=Escherichia coli, GI1790835, Length=486, Percent_Identity=23.4567901234568, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI48994988, Length=200, Percent_Identity=25, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17533571, Length=706, Percent_Identity=28.4702549575071, Blast_Score=287, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17556745, Length=711, Percent_Identity=23.0661040787623, Blast_Score=163, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6323098, Length=712, Percent_Identity=27.6685393258427, Blast_Score=288, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6322359, Length=781, Percent_Identity=24.0717029449424, Blast_Score=207, Evalue=5e-54,
Organism=Drosophila melanogaster, GI24582462, Length=707, Percent_Identity=27.4398868458274, Blast_Score=271, Evalue=8e-73,
Organism=Drosophila melanogaster, GI221458488, Length=741, Percent_Identity=25.5060728744939, Blast_Score=198, Evalue=9e-51,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 74260; Mature: 74260

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYSPDAIRTIALLGHAGCGKTSLAEALLHKGGALHAPGSVERGSTVSDFDPLERKYHHS
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LTSAVLHVDFRNTRIYLIDTPGYPDFSGQAISALPAVETAAIVINAQTGIEMTTRRAMAW
HHHEEEEEEECCCEEEEEECCCCCCCCCCHHHHCCCCCEEEEEEECCCCCHHHHHHHHHH
AQARKLCRMIIVNGIDGEKVDLPALLADIQEAFGKECLPINLPAAGGSQVVDCFFNPAGE
HHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCEEHEECCCCCC
ADFLSVPAAHDALVDQVIEIDPELMELYLEQGEAIQPEQLHAPFERALREGHLVPICFTS
CCEEECCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHCCHHHHHHHCCCEEEEEEEC
AATGAGVPELLDAFVRLLPNPTEGNPPMFYRSVGEGSDAAERKKEVRAEPVPDKHVLAHV
CCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCCCHHHHHHHH
FKIVMDPYVGKMAVFRIHQGTVTRDSQLYIGDARQPIKVAHLLLLQGKEHEEVPRAGPGD
HHHHHCCCCCCEEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
ICAVAKIDEIGFDAVLHDANEDGNIHLIPLEFPTPIYGLAIEPARRGNEQRMAEVLHKLS
EEEEEEECCCCCCCEEECCCCCCCEEEEEECCCCCEEEEEECHHHCCCHHHHHHHHHHHC
AEDPCLRVEHPPGTNETVLLGLGEFHLRCVMERLTEQYKLEVVTRPPRIAYRETIGGSAE
CCCCEEEEECCCCCCCEEEEECHHHHHHHHHHHHHHHHEEEEEECCCCEEHHHHCCCCCC
GHHRHKKQTGGAGQFGEVMLRVDPLPRGTGFEFVDAVKGGAIPGQFIPAVEKGIRQALEC
CCHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHC
GPLAGFPMQDVRVTVLDGKSHPVDSKEVAFATAGRKAFVDAVLKARPSVLEPVVDIEVMM
CCCCCCCCCCCEEEEECCCCCCCCCCCEEEECCCHHHHHHHHHHCCCHHHCCCEEEEEEC
PDSSMGDVIGDLSSKRGQVHGTRTGAANTVIVAGKVPLSELNDYQSRLNSLTGGHGSYTI
CCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEE
QFSHYEGVPPGQQDKMAAKHKAQADTGA
EECCCCCCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MHYSPDAIRTIALLGHAGCGKTSLAEALLHKGGALHAPGSVERGSTVSDFDPLERKYHHS
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LTSAVLHVDFRNTRIYLIDTPGYPDFSGQAISALPAVETAAIVINAQTGIEMTTRRAMAW
HHHEEEEEEECCCEEEEEECCCCCCCCCCHHHHCCCCCEEEEEEECCCCCHHHHHHHHHH
AQARKLCRMIIVNGIDGEKVDLPALLADIQEAFGKECLPINLPAAGGSQVVDCFFNPAGE
HHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCEEHEECCCCCC
ADFLSVPAAHDALVDQVIEIDPELMELYLEQGEAIQPEQLHAPFERALREGHLVPICFTS
CCEEECCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHCCHHHHHHHCCCEEEEEEEC
AATGAGVPELLDAFVRLLPNPTEGNPPMFYRSVGEGSDAAERKKEVRAEPVPDKHVLAHV
CCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCCCHHHHHHHH
FKIVMDPYVGKMAVFRIHQGTVTRDSQLYIGDARQPIKVAHLLLLQGKEHEEVPRAGPGD
HHHHHCCCCCCEEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
ICAVAKIDEIGFDAVLHDANEDGNIHLIPLEFPTPIYGLAIEPARRGNEQRMAEVLHKLS
EEEEEEECCCCCCCEEECCCCCCCEEEEEECCCCCEEEEEECHHHCCCHHHHHHHHHHHC
AEDPCLRVEHPPGTNETVLLGLGEFHLRCVMERLTEQYKLEVVTRPPRIAYRETIGGSAE
CCCCEEEEECCCCCCCEEEEECHHHHHHHHHHHHHHHHEEEEEECCCCEEHHHHCCCCCC
GHHRHKKQTGGAGQFGEVMLRVDPLPRGTGFEFVDAVKGGAIPGQFIPAVEKGIRQALEC
CCHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHC
GPLAGFPMQDVRVTVLDGKSHPVDSKEVAFATAGRKAFVDAVLKARPSVLEPVVDIEVMM
CCCCCCCCCCCEEEEECCCCCCCCCCCEEEECCCHHHHHHHHHHCCCHHHCCCEEEEEEC
PDSSMGDVIGDLSSKRGQVHGTRTGAANTVIVAGKVPLSELNDYQSRLNSLTGGHGSYTI
CCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEE
QFSHYEGVPPGQQDKMAAKHKAQADTGA
EECCCCCCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]