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The map label for this gene is puuB [H]

Identifier: 73537585

GI number: 73537585

Start: 345881

End: 347320

Strand: Reverse

Name: puuB [H]

Synonym: Reut_B3750

Alternate gene names: 73537585

Gene position: 347320-345881 (Counterclockwise)

Preceding gene: 73537586

Following gene: 73537584

Centisome position: 12.74

GC content: 65.21

Gene sequence:

>1440_bases
ATGGAAGCAGTAGCCACCCGCAATCTCCTCACCGGCGGCCATGCCACCGCGCCGCAGCGGCCGTATGACCCCGCGTACGA
CCCGCTGCGCACGCCTACGCCGGGCCAGGGCCGTGAATATGCGCCGACCTACTGGATCGGCACGGCCGGCGAGCCCCCTG
CCGATGACGGCCCGATCACGCATGACATCGACGTGGACGTCGCCATCATCGGCTCCGGCTTCACGGGCCTGACCAGCGCG
ATCTTTCTCGCGCAGGAGCACGGCATCAAGGCGACCGTGCTCGAAGCGAACCGCGTGAGCTGGGGCTGCAGCACGCGCAA
CGGCGGCCAGGCGCAATGCGCGTCGGGCCGGCTCAAGCGCTCGCAATGGATCCAGCGCTATGGCCTGGATACGGCACTGC
GCCTGCATGAAGAAGTCTGCGACGGCATGCAGACCTTCAAGAGCCTGATCAAGGACATCGACTGCGATCCGCAGCCCGGT
GGCCACCTGTATATTGCACACCGCGCCAAGGCCATGCCTGCGCTCGAAAAGGAAGCGAAGGTCCTGCGCGATATCTTCAA
GTACGATGCGCGCATCATCGATGCCGACACCGTGCGCCGCGAGTACGTCGATGACAAGGAAGCCGCGGGCGCCATGCACG
AGCCCGAGGGCATCGGCATCCACGCCGGCAAGCTCGCGTTCGGTTACCTGCGCCGCGCGCGCGAACTCGGCGTCAAGGTG
CATCCGTCGAGCCCGGTGACGGGCTGGGAGACGCGCAATGGCGTGCATTACCTGCGTACGCCGGGCGGCATCGTGCGCGC
GCGCTCGGTAGGCGTGGCCACGGGCGGCTATACCTCGCAGTCGCTGCACCCGCAGCTCAAGAACCGGCTGTTCCCGATCC
TGTCGAACTCCATCGTCACGCGCGTGCTGACGCCCGCCGAGATTGAAGCGTGCAACTTCCGCACGCACCAGGTCATCACC
GACACGCGCATCCTGCGCCACTACTACCGCCTGATGCCCGACGGGCGCCTGCAGATCGGCAGCCGCAGCGCGATCACCGG
TCATGATGCACCGCAAGACCAGTACCGCCAGAAGCTCGTCGACGACATGTACCGCAAATTTCCGTCGTTGCGCGGCATCC
AGATCGACTATTCGTGGTGGGGCTGGGTCGACGTGAGCCACGACATGATGCCCCGCATCACCCAGCCCGATCCGTCGCAG
TCGATCTATTACGCGTTGGGTTATGGCGGCAACGGCGTGATGTATTCCGCGCAGGCCGGCAAACGGCTTGCGGCGCTGAT
TGCTGGCAAAGGCGATTCACTGCCGCCGCTGCCGATCTTCCGTTCGTCGCTGCCCTTCCCGAATGTGCGCGAGATGGTGG
AATCGCAGATGTTCGCGCCGTTCCGCCGCTTTGGCCAGCGCTTCCTGTATCACTGGTACCACTTCAAGGACGAGGTATAA

Upstream 100 bases:

>100_bases
GACGTGTTCACGTTCCACGACGGCAAGATCGCGGTGAAGAACGCGTACCGCAAGGATCGCCCGCCGGTGACCAACGCGGC
CTGAGCACCGGAGACGCGCC

Downstream 100 bases:

>100_bases
CGCCCTAACGCCCCGGCGGCAGGCTTCGCTGCGTCGCAGCATGCCGGCCGACCGCGCTGTGGGCCGCAGCGCGAGTGCAC
AGGACGGGACTCCTTTGTCT

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MEAVATRNLLTGGHATAPQRPYDPAYDPLRTPTPGQGREYAPTYWIGTAGEPPADDGPITHDIDVDVAIIGSGFTGLTSA
IFLAQEHGIKATVLEANRVSWGCSTRNGGQAQCASGRLKRSQWIQRYGLDTALRLHEEVCDGMQTFKSLIKDIDCDPQPG
GHLYIAHRAKAMPALEKEAKVLRDIFKYDARIIDADTVRREYVDDKEAAGAMHEPEGIGIHAGKLAFGYLRRARELGVKV
HPSSPVTGWETRNGVHYLRTPGGIVRARSVGVATGGYTSQSLHPQLKNRLFPILSNSIVTRVLTPAEIEACNFRTHQVIT
DTRILRHYYRLMPDGRLQIGSRSAITGHDAPQDQYRQKLVDDMYRKFPSLRGIQIDYSWWGWVDVSHDMMPRITQPDPSQ
SIYYALGYGGNGVMYSAQAGKRLAALIAGKGDSLPPLPIFRSSLPFPNVREMVESQMFAPFRRFGQRFLYHWYHFKDEV

Sequences:

>Translated_479_residues
MEAVATRNLLTGGHATAPQRPYDPAYDPLRTPTPGQGREYAPTYWIGTAGEPPADDGPITHDIDVDVAIIGSGFTGLTSA
IFLAQEHGIKATVLEANRVSWGCSTRNGGQAQCASGRLKRSQWIQRYGLDTALRLHEEVCDGMQTFKSLIKDIDCDPQPG
GHLYIAHRAKAMPALEKEAKVLRDIFKYDARIIDADTVRREYVDDKEAAGAMHEPEGIGIHAGKLAFGYLRRARELGVKV
HPSSPVTGWETRNGVHYLRTPGGIVRARSVGVATGGYTSQSLHPQLKNRLFPILSNSIVTRVLTPAEIEACNFRTHQVIT
DTRILRHYYRLMPDGRLQIGSRSAITGHDAPQDQYRQKLVDDMYRKFPSLRGIQIDYSWWGWVDVSHDMMPRITQPDPSQ
SIYYALGYGGNGVMYSAQAGKRLAALIAGKGDSLPPLPIFRSSLPFPNVREMVESQMFAPFRRFGQRFLYHWYHFKDEV
>Mature_479_residues
MEAVATRNLLTGGHATAPQRPYDPAYDPLRTPTPGQGREYAPTYWIGTAGEPPADDGPITHDIDVDVAIIGSGFTGLTSA
IFLAQEHGIKATVLEANRVSWGCSTRNGGQAQCASGRLKRSQWIQRYGLDTALRLHEEVCDGMQTFKSLIKDIDCDPQPG
GHLYIAHRAKAMPALEKEAKVLRDIFKYDARIIDADTVRREYVDDKEAAGAMHEPEGIGIHAGKLAFGYLRRARELGVKV
HPSSPVTGWETRNGVHYLRTPGGIVRARSVGVATGGYTSQSLHPQLKNRLFPILSNSIVTRVLTPAEIEACNFRTHQVIT
DTRILRHYYRLMPDGRLQIGSRSAITGHDAPQDQYRQKLVDDMYRKFPSLRGIQIDYSWWGWVDVSHDMMPRITQPDPSQ
SIYYALGYGGNGVMYSAQAGKRLAALIAGKGDSLPPLPIFRSSLPFPNVREMVESQMFAPFRRFGQRFLYHWYHFKDEV

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=421, Percent_Identity=32.7790973871734, Blast_Score=174, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 53490; Mature: 53490

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAVATRNLLTGGHATAPQRPYDPAYDPLRTPTPGQGREYAPTYWIGTAGEPPADDGPIT
CCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEE
HDIDVDVAIIGSGFTGLTSAIFLAQEHGIKATVLEANRVSWGCSTRNGGQAQCASGRLKR
EECCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCCHHHCCHHHH
SQWIQRYGLDTALRLHEEVCDGMQTFKSLIKDIDCDPQPGGHLYIAHRAKAMPALEKEAK
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHH
VLRDIFKYDARIIDADTVRREYVDDKEAAGAMHEPEGIGIHAGKLAFGYLRRARELGVKV
HHHHHHHHCCEEECHHHHHHHHCCCHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHCCEEE
HPSSPVTGWETRNGVHYLRTPGGIVRARSVGVATGGYTSQSLHPQLKNRLFPILSNSIVT
CCCCCCCCCCCCCCEEEEECCCCEEEEEECEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
RVLTPAEIEACNFRTHQVITDTRILRHYYRLMPDGRLQIGSRSAITGHDAPQDQYRQKLV
HHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHH
DDMYRKFPSLRGIQIDYSWWGWVDVSHDMMPRITQPDPSQSIYYALGYGGNGVMYSAQAG
HHHHHHCCCCCCEEEECCEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCEEEECCCC
KRLAALIAGKGDSLPPLPIFRSSLPFPNVREMVESQMFAPFRRFGQRFLYHWYHFKDEV
CEEEEEEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEAVATRNLLTGGHATAPQRPYDPAYDPLRTPTPGQGREYAPTYWIGTAGEPPADDGPIT
CCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEE
HDIDVDVAIIGSGFTGLTSAIFLAQEHGIKATVLEANRVSWGCSTRNGGQAQCASGRLKR
EECCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCCHHHCCHHHH
SQWIQRYGLDTALRLHEEVCDGMQTFKSLIKDIDCDPQPGGHLYIAHRAKAMPALEKEAK
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHH
VLRDIFKYDARIIDADTVRREYVDDKEAAGAMHEPEGIGIHAGKLAFGYLRRARELGVKV
HHHHHHHHCCEEECHHHHHHHHCCCHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHCCEEE
HPSSPVTGWETRNGVHYLRTPGGIVRARSVGVATGGYTSQSLHPQLKNRLFPILSNSIVT
CCCCCCCCCCCCCCEEEEECCCCEEEEEECEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
RVLTPAEIEACNFRTHQVITDTRILRHYYRLMPDGRLQIGSRSAITGHDAPQDQYRQKLV
HHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHH
DDMYRKFPSLRGIQIDYSWWGWVDVSHDMMPRITQPDPSQSIYYALGYGGNGVMYSAQAG
HHHHHHCCCCCCEEEECCEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCEEEECCCC
KRLAALIAGKGDSLPPLPIFRSSLPFPNVREMVESQMFAPFRRFGQRFLYHWYHFKDEV
CEEEEEEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]