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The map label for this gene is rimO

Identifier: 71908364

GI number: 71908364

Start: 2945820

End: 2947142

Strand: Reverse

Name: rimO

Synonym: Daro_2751

Alternate gene names: 71908364

Gene position: 2947142-2945820 (Counterclockwise)

Preceding gene: 71908365

Following gene: 71908363

Centisome position: 65.48

GC content: 61.38

Gene sequence:

>1323_bases
ATGACTATTCAACAAAAAGTGCCGACCGTCGGCTTCGTTTCTCTCGGTTGCCCCAAGGCTAGCAGTGACGCCGAGCGTAT
CCTGACCAAGCTGCGCGCCGAAGGCTACGAGATTTCGCCGAGTTACGACAATTCCGATCTGGTGATCGTCAATACTTGCG
GCTTCATCGATGCTGCAGTCGAAGAGTCGCTCGACGCCATCGGCGAGGCACTCAACGAGAACGGCAAGGTGATCGTCACC
GGCTGTCTCGGCGCCAAGGGCGACATCGTCCAGACGACGCATCCGGCCGTGCTCGCCGTCACCGGCCCGCATGCCGCCGA
CGAGGTCATGGGGATCGTCCACACCCATCTGCCCAAGCCGCACGACCCGTATTCCGATCTGGTGCCACCGCAGGGTGTTC
GCCTGACCCCGGACCACTTTGCCTATCTGAAGATTTCTGAAGGCTGCAACCACAGCTGCACCTTCTGCATCATCCCGTCG
CTGCGCGGCCCGCTGGTCTCAAGGCCGGTTGGCGATGTGCTGGCCGAAGCCGAGAATCTGGCCCGCGCCGGGGTCAAGGA
AATTCTGGTCATTTCGCAGGACACCAGCGCCTACGGCGTCGATCTCAAGTACCGCACCGCCTTCTGGGGCGGCAAGCCGG
TCAAGTCGCGCCTGAAGGAATTGTGCGAGGCGCTGGCCAGTTTAGGCATCTGGGTCCGCCTGCATTACGTTTACCCGTAC
CCGTCGGTCGATGACGTCATTCCGCTGATGGCCGAGGGCAAGATCCTGCCTTACCTCGACGTACCTTTCCAGCACGCCAG
CCCGAAGATCCTGAAGGCGATGAAGCGCCCAGCCTCGGCCGAGAACACGCTGGAACGCATCGCCAAGTGGCGCGAAATCT
GCCCGGAAATCGTCATCCGCTCGACCTTCATCACCGGCTTCCCCGGCGAGACGGAAGAGGACTTCGACCAGCTGATCCAG
TTCCTGGAAGATGCCAAGCTCGACCGCGTCGGTGCTTTTGCTTACTCGCCGGTCGACGGTGCCAAGGCCAACGAACTCGG
TGAGCTGCTGCCGGAAGACGTGCGCGAGGATCGCCGTCGCTGGCTGATGCAGGTCCAGGAAGACATTTCCGCCGACAAGC
TGGCGGCCAAGATCGACACGGTCATTCAGGTGCTGGTCGACGAGGTCGATGAAGAAGGCACCATCGCCCGTTCCAAGGCC
GATGCGCCGGAAATCGACGGACTGGTTTACCTGGATGGTCATTTCGATGCCCAGCCGGGCGACTTCCTGCAGGTCAAGGT
CATTGATGCCGACCACCATGACCTCTACGCCCAAGTCGTCTGA

Upstream 100 bases:

>100_bases
TTTTGATGCACTGAAACAGGCACTTGCCCGCCTGCCAAGCTGAATCCCCCGTAAAATACCCCCTTTGTTCGCCGGCCTGT
CCGGATAAGGTTCCATTCGC

Downstream 100 bases:

>100_bases
TCTGAAATCAATCCTGGCCGGGATCGTCGGTACCGCTCAGGTGCTGACTGACCCGGTCGAGTTGGCGCCGTTTCTGACAG
ACTGGCGCGGTCGTTATCGC

Product: hypothetical protein

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MTIQQKVPTVGFVSLGCPKASSDAERILTKLRAEGYEISPSYDNSDLVIVNTCGFIDAAVEESLDAIGEALNENGKVIVT
GCLGAKGDIVQTTHPAVLAVTGPHAADEVMGIVHTHLPKPHDPYSDLVPPQGVRLTPDHFAYLKISEGCNHSCTFCIIPS
LRGPLVSRPVGDVLAEAENLARAGVKEILVISQDTSAYGVDLKYRTAFWGGKPVKSRLKELCEALASLGIWVRLHYVYPY
PSVDDVIPLMAEGKILPYLDVPFQHASPKILKAMKRPASAENTLERIAKWREICPEIVIRSTFITGFPGETEEDFDQLIQ
FLEDAKLDRVGAFAYSPVDGAKANELGELLPEDVREDRRRWLMQVQEDISADKLAAKIDTVIQVLVDEVDEEGTIARSKA
DAPEIDGLVYLDGHFDAQPGDFLQVKVIDADHHDLYAQVV

Sequences:

>Translated_440_residues
MTIQQKVPTVGFVSLGCPKASSDAERILTKLRAEGYEISPSYDNSDLVIVNTCGFIDAAVEESLDAIGEALNENGKVIVT
GCLGAKGDIVQTTHPAVLAVTGPHAADEVMGIVHTHLPKPHDPYSDLVPPQGVRLTPDHFAYLKISEGCNHSCTFCIIPS
LRGPLVSRPVGDVLAEAENLARAGVKEILVISQDTSAYGVDLKYRTAFWGGKPVKSRLKELCEALASLGIWVRLHYVYPY
PSVDDVIPLMAEGKILPYLDVPFQHASPKILKAMKRPASAENTLERIAKWREICPEIVIRSTFITGFPGETEEDFDQLIQ
FLEDAKLDRVGAFAYSPVDGAKANELGELLPEDVREDRRRWLMQVQEDISADKLAAKIDTVIQVLVDEVDEEGTIARSKA
DAPEIDGLVYLDGHFDAQPGDFLQVKVIDADHHDLYAQVV
>Mature_439_residues
TIQQKVPTVGFVSLGCPKASSDAERILTKLRAEGYEISPSYDNSDLVIVNTCGFIDAAVEESLDAIGEALNENGKVIVTG
CLGAKGDIVQTTHPAVLAVTGPHAADEVMGIVHTHLPKPHDPYSDLVPPQGVRLTPDHFAYLKISEGCNHSCTFCIIPSL
RGPLVSRPVGDVLAEAENLARAGVKEILVISQDTSAYGVDLKYRTAFWGGKPVKSRLKELCEALASLGIWVRLHYVYPYP
SVDDVIPLMAEGKILPYLDVPFQHASPKILKAMKRPASAENTLERIAKWREICPEIVIRSTFITGFPGETEEDFDQLIQF
LEDAKLDRVGAFAYSPVDGAKANELGELLPEDVREDRRRWLMQVQEDISADKLAAKIDTVIQVLVDEVDEEGTIARSKAD
APEIDGLVYLDGHFDAQPGDFLQVKVIDADHHDLYAQVV

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=485, Percent_Identity=24.5360824742268, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI28872782, Length=485, Percent_Identity=24.5360824742268, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI93277076, Length=392, Percent_Identity=26.2755102040816, Blast_Score=129, Evalue=5e-30,
Organism=Escherichia coli, GI1787057, Length=434, Percent_Identity=66.8202764976959, Blast_Score=616, Evalue=1e-178,
Organism=Escherichia coli, GI1786882, Length=452, Percent_Identity=26.7699115044248, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17553146, Length=484, Percent_Identity=23.7603305785124, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71996771, Length=223, Percent_Identity=30.4932735426009, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356207, Length=479, Percent_Identity=24.0083507306889, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI19922432, Length=398, Percent_Identity=25.3768844221106, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_DECAR (Q47CF0)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_285951.1
- ProteinModelPortal:   Q47CF0
- SMR:   Q47CF0
- STRING:   Q47CF0
- GeneID:   3566805
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_2751
- NMPDR:   fig|159087.4.peg.3882
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- BioCyc:   DARO159087:DARO_2751-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 48189; Mature: 48058

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIQQKVPTVGFVSLGCPKASSDAERILTKLRAEGYEISPSYDNSDLVIVNTCGFIDAAV
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCEEECCCCCCCCEEEEEECCCHHHHH
EESLDAIGEALNENGKVIVTGCLGAKGDIVQTTHPAVLAVTGPHAADEVMGIVHTHLPKP
HHHHHHHHHHHCCCCCEEEEEECCCCCCEEECCCCEEEEEECCCHHHHHHHHHHHCCCCC
HDPYSDLVPPQGVRLTPDHFAYLKISEGCNHSCTFCIIPSLRGPLVSRPVGDVLAEAENL
CCCHHHCCCCCCCEECCCCEEEEEECCCCCCCEEEEEECCCCCCHHCCCHHHHHHHHHHH
ARAGVKEILVISQDTSAYGVDLKYRTAFWGGKPVKSRLKELCEALASLGIWVRLHYVYPY
HHCCHHEEEEEECCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEEEEECC
PSVDDVIPLMAEGKILPYLDVPFQHASPKILKAMKRPASAENTLERIAKWREICPEIVIR
CCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
STFITGFPGETEEDFDQLIQFLEDAKLDRVGAFAYSPVDGAKANELGELLPEDVREDRRR
HHEEECCCCCCHHHHHHHHHHHHHCCHHHHCCEEECCCCCCCHHHHHHHCHHHHHHHHHH
WLMQVQEDISADKLAAKIDTVIQVLVDEVDEEGTIARSKADAPEIDGLVYLDGHFDAQPG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCEEEECCCCCCCCC
DFLQVKVIDADHHDLYAQVV
CEEEEEEEECCCCCEEEEEC
>Mature Secondary Structure 
TIQQKVPTVGFVSLGCPKASSDAERILTKLRAEGYEISPSYDNSDLVIVNTCGFIDAAV
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCEEECCCCCCCCEEEEEECCCHHHHH
EESLDAIGEALNENGKVIVTGCLGAKGDIVQTTHPAVLAVTGPHAADEVMGIVHTHLPKP
HHHHHHHHHHHCCCCCEEEEEECCCCCCEEECCCCEEEEEECCCHHHHHHHHHHHCCCCC
HDPYSDLVPPQGVRLTPDHFAYLKISEGCNHSCTFCIIPSLRGPLVSRPVGDVLAEAENL
CCCHHHCCCCCCCEECCCCEEEEEECCCCCCCEEEEEECCCCCCHHCCCHHHHHHHHHHH
ARAGVKEILVISQDTSAYGVDLKYRTAFWGGKPVKSRLKELCEALASLGIWVRLHYVYPY
HHCCHHEEEEEECCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEEEEECC
PSVDDVIPLMAEGKILPYLDVPFQHASPKILKAMKRPASAENTLERIAKWREICPEIVIR
CCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
STFITGFPGETEEDFDQLIQFLEDAKLDRVGAFAYSPVDGAKANELGELLPEDVREDRRR
HHEEECCCCCCHHHHHHHHHHHHHCCHHHHCCEEECCCCCCCHHHHHHHCHHHHHHHHHH
WLMQVQEDISADKLAAKIDTVIQVLVDEVDEEGTIARSKADAPEIDGLVYLDGHFDAQPG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCEEEECCCCCCCCC
DFLQVKVIDADHHDLYAQVV
CEEEEEEEECCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA