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The map label for this gene is pcm

Identifier: 71908139

GI number: 71908139

Start: 2720857

End: 2721507

Strand: Reverse

Name: pcm

Synonym: Daro_2523

Alternate gene names: 71908139

Gene position: 2721507-2720857 (Counterclockwise)

Preceding gene: 71908140

Following gene: 71908138

Centisome position: 60.46

GC content: 55.61

Gene sequence:

>651_bases
GTGTTGCACGGTATTGGCATGACTTCGCAGCGGACGCGGGCGCGCATGATTGAGCGCCTGCGCGAGAAGGGGATTCGCAA
CGAGGCGGTTCTCAAGGCAATGGCGGCTGTTCCTCGGCATGTCTTCGTTGAAGAGGCTTTGGCTTCGCGGGCTTATGAAG
ACACTGCGCTACCGCTTGGCATGGGGCAGACTATTTCCCAGCCTTTTGTCGTGGCTCGGATGATCGAACTCTTGCTCAAC
GGGCGAAGTTCTTTGGGTAAAACCCTGGAGGTCGGTGCAGGTTGTGGTTATCAAGCTGCCGTTCTGGCGCAATTGACCAA
GGATGTCTATGCCGTTGAGCGGCTTGGTCCATTGCTGGAAAAGGCCAAAGCCAACATGCGGATCTTGCAGCAGTTCAATG
TTCGCCTTAAACATGCCGATGGACAACTTGGCTTGCCCGAAGCCGGGCCATTTGACAGTATCATAGTCGCTGCTGCCGGT
TCCCACGTTCCTCCTGCACTGCTCGAACAGCTTGCGCCAGGTGGGCGTTTGGTCTTGCCAGTTGGGGCTGGTGAACAGTA
TCTTAGCTTTATCGAACATACGCCTCAGGGATATGTCGAAACGCGGCTTGATGCCGTACGTTTCGTCCCGCTACTTTCAG
GAACGCAATGA

Upstream 100 bases:

>100_bases
CGGTCGAACGAGGCTTTGTTTCGATTACGCCCTTGCAGATTGATCTGACGCATTCGGCGCAGCTATCCCATATCGATCAG
TGGATGAAGTGAGTCAAGGC

Downstream 100 bases:

>100_bases
TTCGAATCGCCGCTGTAATTCTTTCTACACTGCTTCTGGCTGGTTGCATGTCTCAGCAGCCGGTGCCGGCTGTGGACCGC
TCGGGAACGGCACAATCCAG

Product: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MLHGIGMTSQRTRARMIERLREKGIRNEAVLKAMAAVPRHVFVEEALASRAYEDTALPLGMGQTISQPFVVARMIELLLN
GRSSLGKTLEVGAGCGYQAAVLAQLTKDVYAVERLGPLLEKAKANMRILQQFNVRLKHADGQLGLPEAGPFDSIIVAAAG
SHVPPALLEQLAPGGRLVLPVGAGEQYLSFIEHTPQGYVETRLDAVRFVPLLSGTQ

Sequences:

>Translated_216_residues
MLHGIGMTSQRTRARMIERLREKGIRNEAVLKAMAAVPRHVFVEEALASRAYEDTALPLGMGQTISQPFVVARMIELLLN
GRSSLGKTLEVGAGCGYQAAVLAQLTKDVYAVERLGPLLEKAKANMRILQQFNVRLKHADGQLGLPEAGPFDSIIVAAAG
SHVPPALLEQLAPGGRLVLPVGAGEQYLSFIEHTPQGYVETRLDAVRFVPLLSGTQ
>Mature_216_residues
MLHGIGMTSQRTRARMIERLREKGIRNEAVLKAMAAVPRHVFVEEALASRAYEDTALPLGMGQTISQPFVVARMIELLLN
GRSSLGKTLEVGAGCGYQAAVLAQLTKDVYAVERLGPLLEKAKANMRILQQFNVRLKHADGQLGLPEAGPFDSIIVAAAG
SHVPPALLEQLAPGGRLVLPVGAGEQYLSFIEHTPQGYVETRLDAVRFVPLLSGTQ

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=212, Percent_Identity=33.0188679245283, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1789100, Length=209, Percent_Identity=46.88995215311, Blast_Score=179, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI71983477, Length=218, Percent_Identity=31.651376146789, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI193207222, Length=218, Percent_Identity=30.7339449541284, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI17981723, Length=213, Percent_Identity=32.3943661971831, Blast_Score=94, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_DECAR (Q47D25)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_285726.1
- ProteinModelPortal:   Q47D25
- SMR:   Q47D25
- STRING:   Q47D25
- GeneID:   3567557
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_2523
- eggNOG:   COG2518
- HOGENOM:   HBG699907
- OMA:   YSVERIR
- ProtClustDB:   CLSK752525
- BioCyc:   DARO159087:DARO_2523-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 23317; Mature: 23317

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 66-66

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHGIGMTSQRTRARMIERLREKGIRNEAVLKAMAAVPRHVFVEEALASRAYEDTALPLG
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
MGQTISQPFVVARMIELLLNGRSSLGKTLEVGAGCGYQAAVLAQLTKDVYAVERLGPLLE
CCCHHCHHHHHHHHHHHHHCCCHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KAKANMRILQQFNVRLKHADGQLGLPEAGPFDSIIVAAAGSHVPPALLEQLAPGGRLVLP
HHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHEECCCCCCCHHHHHHHCCCCEEEEE
VGAGEQYLSFIEHTPQGYVETRLDAVRFVPLLSGTQ
ECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLHGIGMTSQRTRARMIERLREKGIRNEAVLKAMAAVPRHVFVEEALASRAYEDTALPLG
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
MGQTISQPFVVARMIELLLNGRSSLGKTLEVGAGCGYQAAVLAQLTKDVYAVERLGPLLE
CCCHHCHHHHHHHHHHHHHCCCHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KAKANMRILQQFNVRLKHADGQLGLPEAGPFDSIIVAAAGSHVPPALLEQLAPGGRLVLP
HHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHEECCCCCCCHHHHHHHCCCCEEEEE
VGAGEQYLSFIEHTPQGYVETRLDAVRFVPLLSGTQ
ECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA