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The map label for this gene is cusS [H]

Identifier: 71908091

GI number: 71908091

Start: 2671798

End: 2673165

Strand: Reverse

Name: cusS [H]

Synonym: Daro_2473

Alternate gene names: 71908091

Gene position: 2673165-2671798 (Counterclockwise)

Preceding gene: 71908092

Following gene: 71908088

Centisome position: 59.39

GC content: 61.18

Gene sequence:

>1368_bases
GTGCCGGGCTGGCGGTATTCGATCACGCTGCGCCTGTCGCTGGCCTTCGCGCTGCTGGCGACGCTCGTGTTCGCAGCGCT
CGGTCTGTATTTCAGCCGCGCTGCCGATGACCACATGGCCGAACTGGATGCCCACGATCTGTTCGGCAAGCTGGCGCTGA
TTGGCCATGTTGGCGCGCAAGAGGAAACGGCGGAAGCCTTCGCAGCCCGGCTCGGCGATGCCCTGATTGGCCAGAATGGG
GTAATTGCGACAGTCGATGCCCAAAAAAGGGCTGTTTTCAGATGGCCGTCAGCCGAACTTGCCGATCCGCTGGCGGCAGC
CGGTGTGGCCTTGAGCCAGAGGCCGATCCGGCAAACCCTGGGCGGCGCCGAGTACAGGGTCATTGCCGCCGATATGGAAA
CCCCTTGGGGTGGCACGGCACACGTCGTGGTCGGCCGCGACATTCGCCACCACACCGATTTTCTCGATCAACTGCAGCAG
GATTTCTGGCTGGCCATCGTGGCTGCGGCCTTGCTGACGGCCGTTGTTGGCGCCCTGATCGTTCGTCGTGGCATGCACCC
GGTGCGCGATATTGCCAGTGCTGCAGGCCGCATCTCCGCCGGGCAACTGGCCGAGCGGATTCCTGAACAGGATGTGCCGA
CGGAATTAGCTGAACTGGTCCATTCCTTCAACGCCATGCTGGGCCGTCTGGAGGAGTCATTCAAGCGCTTGTCTGATTTT
TCGGCCGATCTCGCCCATGAGTTGCGGACGCCGATTCACAGCTTGCGCATGCAGACCGAGGTATCGCTAGGCAAGGCGCG
TAGCGTCAATGAATACCATGATCTGCTGGCGTCGAATCTGGAAGAATACGAACGCCTGTCGCGGATGATTGCCGACATGC
TGTTCCTGGCCAAAGCCGAGCATGGCCTGCTTGTGCCGCAGAAGGCGGCGGTGCCATTGCTCGATCTGTGTCGCCGGCTC
GTTGATTACTATGGCATTCTGGCCGACGATATTGCCATCGAAGTGCTTGGCGAAAACCTGGTGGTTCAAGGCGACAGCCT
GATGCTGGAGCGGGCCATCGGCAATTTGCTGAGCAATGCCATCCTCCATACACCGGCGGGCGGCATGGTCGGATTGTCGG
TGCGCCAGCGAGACGGCGAGGTGGTGCTATCGATTGCTAATACGGGGCAGCCGATCCCGGCTGAGGCCTTGCATGGCATC
TTCGACCGCTTCGTCAGGCTCAATCCCGGTGGTGAGGGGAGTGGCCTTGGGCTGGCGATTGTCAAATCGATCGTGCTGGC
CCACGGTGGCGTTGTTTCGGCGACTTCTGGCGACTCGATGACCGAGTTCGTCATTGAGTTTCTCGATAAGGGCGATCAGC
CGGCCTGA

Upstream 100 bases:

>100_bases
TTGCCATCCGCCGCCTGCGGGCCAAGGTCGATGAGGAGTTCACGCCCAAACTGATCCATACCCTGCGCGGCATGGGCTAT
GTGATGGAGATTCGCTGATC

Downstream 100 bases:

>100_bases
AGCGTGGCCGCGTAGCGCTGTTTTTCGGCGGCGCTCAGTTGCTTGATCCGGTATTCCGCCTTTGAGGCCGATGAGCGATC
GGGATGGGTTTCATGCCCCA

Product: heavy metal sensor kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MPGWRYSITLRLSLAFALLATLVFAALGLYFSRAADDHMAELDAHDLFGKLALIGHVGAQEETAEAFAARLGDALIGQNG
VIATVDAQKRAVFRWPSAELADPLAAAGVALSQRPIRQTLGGAEYRVIAADMETPWGGTAHVVVGRDIRHHTDFLDQLQQ
DFWLAIVAAALLTAVVGALIVRRGMHPVRDIASAAGRISAGQLAERIPEQDVPTELAELVHSFNAMLGRLEESFKRLSDF
SADLAHELRTPIHSLRMQTEVSLGKARSVNEYHDLLASNLEEYERLSRMIADMLFLAKAEHGLLVPQKAAVPLLDLCRRL
VDYYGILADDIAIEVLGENLVVQGDSLMLERAIGNLLSNAILHTPAGGMVGLSVRQRDGEVVLSIANTGQPIPAEALHGI
FDRFVRLNPGGEGSGLGLAIVKSIVLAHGGVVSATSGDSMTEFVIEFLDKGDQPA

Sequences:

>Translated_455_residues
MPGWRYSITLRLSLAFALLATLVFAALGLYFSRAADDHMAELDAHDLFGKLALIGHVGAQEETAEAFAARLGDALIGQNG
VIATVDAQKRAVFRWPSAELADPLAAAGVALSQRPIRQTLGGAEYRVIAADMETPWGGTAHVVVGRDIRHHTDFLDQLQQ
DFWLAIVAAALLTAVVGALIVRRGMHPVRDIASAAGRISAGQLAERIPEQDVPTELAELVHSFNAMLGRLEESFKRLSDF
SADLAHELRTPIHSLRMQTEVSLGKARSVNEYHDLLASNLEEYERLSRMIADMLFLAKAEHGLLVPQKAAVPLLDLCRRL
VDYYGILADDIAIEVLGENLVVQGDSLMLERAIGNLLSNAILHTPAGGMVGLSVRQRDGEVVLSIANTGQPIPAEALHGI
FDRFVRLNPGGEGSGLGLAIVKSIVLAHGGVVSATSGDSMTEFVIEFLDKGDQPA
>Mature_454_residues
PGWRYSITLRLSLAFALLATLVFAALGLYFSRAADDHMAELDAHDLFGKLALIGHVGAQEETAEAFAARLGDALIGQNGV
IATVDAQKRAVFRWPSAELADPLAAAGVALSQRPIRQTLGGAEYRVIAADMETPWGGTAHVVVGRDIRHHTDFLDQLQQD
FWLAIVAAALLTAVVGALIVRRGMHPVRDIASAAGRISAGQLAERIPEQDVPTELAELVHSFNAMLGRLEESFKRLSDFS
ADLAHELRTPIHSLRMQTEVSLGKARSVNEYHDLLASNLEEYERLSRMIADMLFLAKAEHGLLVPQKAAVPLLDLCRRLV
DYYGILADDIAIEVLGENLVVQGDSLMLERAIGNLLSNAILHTPAGGMVGLSVRQRDGEVVLSIANTGQPIPAEALHGIF
DRFVRLNPGGEGSGLGLAIVKSIVLAHGGVVSATSGDSMTEFVIEFLDKGDQPA

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=472, Percent_Identity=34.5338983050847, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI1788279, Length=447, Percent_Identity=28.6353467561521, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI1788393, Length=295, Percent_Identity=32.2033898305085, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1789403, Length=313, Percent_Identity=27.4760383386581, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790346, Length=299, Percent_Identity=25.4180602006689, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790861, Length=224, Percent_Identity=30.3571428571429, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787894, Length=263, Percent_Identity=31.9391634980989, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790551, Length=264, Percent_Identity=26.5151515151515, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790436, Length=214, Percent_Identity=27.5700934579439, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1787374, Length=229, Percent_Identity=28.3842794759825, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI145693157, Length=253, Percent_Identity=28.0632411067194, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786600, Length=201, Percent_Identity=29.8507462686567, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786912, Length=197, Percent_Identity=27.4111675126904, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 48913; Mature: 48782

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGWRYSITLRLSLAFALLATLVFAALGLYFSRAADDHMAELDAHDLFGKLALIGHVGAQ
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCC
EETAEAFAARLGDALIGQNGVIATVDAQKRAVFRWPSAELADPLAAAGVALSQRPIRQTL
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEECCCHHHHCHHHHHHHHHHHHHHHHHH
GGAEYRVIAADMETPWGGTAHVVVGRDIRHHTDFLDQLQQDFWLAIVAAALLTAVVGALI
CCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRRGMHPVRDIASAAGRISAGQLAERIPEQDVPTELAELVHSFNAMLGRLEESFKRLSDF
HHHCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SADLAHELRTPIHSLRMQTEVSLGKARSVNEYHDLLASNLEEYERLSRMIADMLFLAKAE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
HGLLVPQKAAVPLLDLCRRLVDYYGILADDIAIEVLGENLVVQGDSLMLERAIGNLLSNA
CCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHH
ILHTPAGGMVGLSVRQRDGEVVLSIANTGQPIPAEALHGIFDRFVRLNPGGEGSGLGLAI
HHCCCCCCEEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHEECCCCCCCCCHHHHH
VKSIVLAHGGVVSATSGDSMTEFVIEFLDKGDQPA
HHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PGWRYSITLRLSLAFALLATLVFAALGLYFSRAADDHMAELDAHDLFGKLALIGHVGAQ
CCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCC
EETAEAFAARLGDALIGQNGVIATVDAQKRAVFRWPSAELADPLAAAGVALSQRPIRQTL
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEECCCHHHHCHHHHHHHHHHHHHHHHHH
GGAEYRVIAADMETPWGGTAHVVVGRDIRHHTDFLDQLQQDFWLAIVAAALLTAVVGALI
CCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRRGMHPVRDIASAAGRISAGQLAERIPEQDVPTELAELVHSFNAMLGRLEESFKRLSDF
HHHCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SADLAHELRTPIHSLRMQTEVSLGKARSVNEYHDLLASNLEEYERLSRMIADMLFLAKAE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
HGLLVPQKAAVPLLDLCRRLVDYYGILADDIAIEVLGENLVVQGDSLMLERAIGNLLSNA
CCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHH
ILHTPAGGMVGLSVRQRDGEVVLSIANTGQPIPAEALHGIFDRFVRLNPGGEGSGLGLAI
HHCCCCCCEEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHEECCCCCCCCCHHHHH
VKSIVLAHGGVVSATSGDSMTEFVIEFLDKGDQPA
HHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]