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The map label for this gene is amiC [H]

Identifier: 71907075

GI number: 71907075

Start: 1569433

End: 1570695

Strand: Reverse

Name: amiC [H]

Synonym: Daro_1443

Alternate gene names: 71907075

Gene position: 1570695-1569433 (Counterclockwise)

Preceding gene: 71907077

Following gene: 71907074

Centisome position: 34.9

GC content: 59.14

Gene sequence:

>1263_bases
ATGAGCAATCGTCGCAACTTCATCAAGGCAACCGTTCTCGCCGCCGCCCTGTCGTCCATCGGCATGGCCGCCCACGCCGC
CGATACCATCAAGGTCGGCGTACTGCACTCCCTGTCCGGCACCATGGCGATTTCCGAGACCGCGCTCAAGGAAACCGTGC
TGATGACCATCGAGGAAATCAACAAGTCCGGCGGCGTACTCGGCAAGAAGCTCGAGCCGGTCGTCGTCGATCCGGCTTCC
AACTGGCCGCTGTTCGCCGAAAAGGCCCGCCAGCTGCTGACCAAGGACAAGGTTGCCGTCACCTTTGGTTGCTGGACTTC
GGTGTCCCGCAAGTCTGTGCTGCCGGTCTACAAGGAACTGAACGGCCTGCTCTTCTACCCGGTTCAGTACGAGGGCGAAG
AACTGGAAAAAAACGTCTTCTACACCGGCGCTGCGCCCAACCAGCAGGCCATTCCGGCCGTCGAATACTTGATGAGTAAG
GATGGCGGCGAAGCCAAGCGATTCGTGCTGCTCGGCACCGACTATGTCTATCCGCGTACCACCAACAAGATTCTGCGCGC
CTTCCTGAAGTCCAAAGGCGTGGCCGACGCCGACATCATGGAAGACTACACGCCGTTCGGCCACAGCGATTACCAGACCA
TCATCGCCAAGATCAAGAAGTTCGCTTCCGAAGGCAAGAAGACCGCCGTCGTCTCGACCATCAACGGCGATTCCAACGTG
CCGTTCTACAAGGAACTGGGCAATGCCGGCCTGAAGGCCACCGATGTGCCGGTTGTCGCCTTCTCGGTCGGTGAAGAAGA
GCTGCGCGGTGTCGATACCAAGCCGCTGGTTGGCCACCTGGCTTCGTGGAACTACTTCATGTCGCTGAAGAATCCGGAAA
ACGACAAGTTCGTGAAGATGTACCGCGAATGGGCCAAGAAGGCCAAGCTGCCAAATGCCGACAAGGTCGTGACGAATGAC
CCGATGGAAGCTACCTACATCGGCATCCACATGTGGAAGCAGGCTGTCGAGAAGGCCAAGTCCACCGACACCGACAAGGT
TATCGCCGCCATGGCCGGCCAGACCTTCAAGGCACCGAGCGGCATCCTGTCGAAGATGGACGAGAAGAACCACCACCTGC
ACAAGTCGGTGTTCATCGGCGAAGTGAAGGCCGATGGCCAGTTCAATGTCGTCTGGAAGACGCCCGGCCCGGTCAAGGCC
CAGCCGTGGAGCCCGTACATCGCCGGCAATGACAAGAAGAAGGACGAGCCGGAAGCGAAGTGA

Upstream 100 bases:

>100_bases
CCTACGCAATCTAACGTATTTATCGCGGGTAACCCCGGCCGTAATCTGGCGCTGCACTGCAACAACGCAGACCAGATTAC
CCAACCTCCGAGGAGATTCC

Downstream 100 bases:

>100_bases
TCCGGATCGGTCTGTCAGTCGCAAACGAACGGGGGCCTCGGCCCCCGTTTTTCCAGCCAATCGAAAGGAATGCACCATGA
ACCGCGAAGAAGTGACCGAT

Product: twin-arginine translocation pathway signal

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MSNRRNFIKATVLAAALSSIGMAAHAADTIKVGVLHSLSGTMAISETALKETVLMTIEEINKSGGVLGKKLEPVVVDPAS
NWPLFAEKARQLLTKDKVAVTFGCWTSVSRKSVLPVYKELNGLLFYPVQYEGEELEKNVFYTGAAPNQQAIPAVEYLMSK
DGGEAKRFVLLGTDYVYPRTTNKILRAFLKSKGVADADIMEDYTPFGHSDYQTIIAKIKKFASEGKKTAVVSTINGDSNV
PFYKELGNAGLKATDVPVVAFSVGEEELRGVDTKPLVGHLASWNYFMSLKNPENDKFVKMYREWAKKAKLPNADKVVTND
PMEATYIGIHMWKQAVEKAKSTDTDKVIAAMAGQTFKAPSGILSKMDEKNHHLHKSVFIGEVKADGQFNVVWKTPGPVKA
QPWSPYIAGNDKKKDEPEAK

Sequences:

>Translated_420_residues
MSNRRNFIKATVLAAALSSIGMAAHAADTIKVGVLHSLSGTMAISETALKETVLMTIEEINKSGGVLGKKLEPVVVDPAS
NWPLFAEKARQLLTKDKVAVTFGCWTSVSRKSVLPVYKELNGLLFYPVQYEGEELEKNVFYTGAAPNQQAIPAVEYLMSK
DGGEAKRFVLLGTDYVYPRTTNKILRAFLKSKGVADADIMEDYTPFGHSDYQTIIAKIKKFASEGKKTAVVSTINGDSNV
PFYKELGNAGLKATDVPVVAFSVGEEELRGVDTKPLVGHLASWNYFMSLKNPENDKFVKMYREWAKKAKLPNADKVVTND
PMEATYIGIHMWKQAVEKAKSTDTDKVIAAMAGQTFKAPSGILSKMDEKNHHLHKSVFIGEVKADGQFNVVWKTPGPVKA
QPWSPYIAGNDKKKDEPEAK
>Mature_419_residues
SNRRNFIKATVLAAALSSIGMAAHAADTIKVGVLHSLSGTMAISETALKETVLMTIEEINKSGGVLGKKLEPVVVDPASN
WPLFAEKARQLLTKDKVAVTFGCWTSVSRKSVLPVYKELNGLLFYPVQYEGEELEKNVFYTGAAPNQQAIPAVEYLMSKD
GGEAKRFVLLGTDYVYPRTTNKILRAFLKSKGVADADIMEDYTPFGHSDYQTIIAKIKKFASEGKKTAVVSTINGDSNVP
FYKELGNAGLKATDVPVVAFSVGEEELRGVDTKPLVGHLASWNYFMSLKNPENDKFVKMYREWAKKAKLPNADKVVTNDP
MEATYIGIHMWKQAVEKAKSTDTDKVIAAMAGQTFKAPSGILSKMDEKNHHLHKSVFIGEVKADGQFNVVWKTPGPVKAQ
PWSPYIAGNDKKKDEPEAK

Specific function: Negatively regulates the expression of the aliphatic amidase operon. AmiC functions by inhibiting the action of AmiR at the protein level. It exhibits protein kinase activity [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000709 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 46156; Mature: 46025

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNRRNFIKATVLAAALSSIGMAAHAADTIKVGVLHSLSGTMAISETALKETVLMTIEEI
CCCCHHHHHHHHHHHHHHHHCHHHHCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHH
NKSGGVLGKKLEPVVVDPASNWPLFAEKARQLLTKDKVAVTFGCWTSVSRKSVLPVYKEL
CCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHC
NGLLFYPVQYEGEELEKNVFYTGAAPNQQAIPAVEYLMSKDGGEAKRFVLLGTDYVYPRT
CCEEEEEEECCCCHHCCCEEEECCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCEECCCC
TNKILRAFLKSKGVADADIMEDYTPFGHSDYQTIIAKIKKFASEGKKTAVVSTINGDSNV
HHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
PFYKELGNAGLKATDVPVVAFSVGEEELRGVDTKPLVGHLASWNYFMSLKNPENDKFVKM
CHHHHHCCCCCEECCCCEEEEECCHHHHCCCCCCHHHHHHHCCEEEEEECCCCCHHHHHH
YREWAKKAKLPNADKVVTNDPMEATYIGIHMWKQAVEKAKSTDTDKVIAAMAGQTFKAPS
HHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCH
GILSKMDEKNHHLHKSVFIGEVKADGQFNVVWKTPGPVKAQPWSPYIAGNDKKKDEPEAK
HHHHHHHHHCCHHHHHEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNRRNFIKATVLAAALSSIGMAAHAADTIKVGVLHSLSGTMAISETALKETVLMTIEEI
CCCHHHHHHHHHHHHHHHHCHHHHCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHH
NKSGGVLGKKLEPVVVDPASNWPLFAEKARQLLTKDKVAVTFGCWTSVSRKSVLPVYKEL
CCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHC
NGLLFYPVQYEGEELEKNVFYTGAAPNQQAIPAVEYLMSKDGGEAKRFVLLGTDYVYPRT
CCEEEEEEECCCCHHCCCEEEECCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCEECCCC
TNKILRAFLKSKGVADADIMEDYTPFGHSDYQTIIAKIKKFASEGKKTAVVSTINGDSNV
HHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
PFYKELGNAGLKATDVPVVAFSVGEEELRGVDTKPLVGHLASWNYFMSLKNPENDKFVKM
CHHHHHCCCCCEECCCCEEEEECCHHHHCCCCCCHHHHHHHCCEEEEEECCCCCHHHHHH
YREWAKKAKLPNADKVVTNDPMEATYIGIHMWKQAVEKAKSTDTDKVIAAMAGQTFKAPS
HHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCH
GILSKMDEKNHHLHKSVFIGEVKADGQFNVVWKTPGPVKAQPWSPYIAGNDKKKDEPEAK
HHHHHHHHHCCHHHHHEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1907262; 10984043; 1762155; 7813419; 10508151; 10708652 [H]