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The map label for this gene is pleD [H]

Identifier: 71907077

GI number: 71907077

Start: 1572557

End: 1573483

Strand: Reverse

Name: pleD [H]

Synonym: Daro_1445

Alternate gene names: 71907077

Gene position: 1573483-1572557 (Counterclockwise)

Preceding gene: 71907078

Following gene: 71907075

Centisome position: 34.96

GC content: 66.67

Gene sequence:

>927_bases
ATGCCACCCGTTGATCGCAACATCTCGGACATCGTCCTGATCGTCGACGATGTGCCGGAAAACCTCTCCCTGCTGCACGA
TGCGTTGGACGAGGCCGGCTACACCGTGCTCGTCGCGACGCACGGCGAAGCGGCGCTGCAACGCGCCCGCCAGAGCCTGC
CCGACGTCATCCTGCTCGACGCGCTGATGCCGGGCATGGACGGTTTCGAGGTCGCCCGCCGCCTGAAAGCCGACTTCTCG
ACCCAGCACATCCCCATCGTCTTCATGACCGGGCTGACCGAAACCGAGCACGTCGTCGCCGCCTTTGCCGCGGGCGGTGC
CGATTACGTCACCAAGCCGATCCGCCCGGCCGAGGTACTGGCCCGCATTGCCGCCCACATGATGAACGCCCGGCAGATGA
AGCAGGCGCGCAGCGCGCTCGACGCCTTCGGCCAGGCCACGGTCGCCGTGCGCGCCGCCGACAGCCGTATCGTCTGGCAG
ACGCCGCTCGCCCGCGGCCTGCTGAACAGCTATTTCGCCAACCCGGAAACCGTCGCCCCGGAGGAACTGCTGACCTGGAT
CACGGCGGCACTCCGCGCCCGGGCCGATGGCCGTGAGCCCGCGCCGCTGCTGATCGCCGAAGGCAACAAGCGCCTGCTCG
CCTCCTTCCATGACCAGACCGGCGACGATGAATGGATGGTCGTGCTGCGCGAAGAAAACGACGCCTCAGCGGTCGAATCG
CTGATCGCCGCCTTCCGCCTGACCCAGCGCGAAGCCGAAGTGCTCTACTGGGTGGTACAGGGCAAGACCAGCAACGATAT
CGGCGACATCCTCGGCAACAGCCCGCGGACGGTCAACAAGCATCTGGAGCATGTTTTCGAGAAGCTCGGCGTCGAAACGC
GGACGGCAGCCGCCAATCTGGCGATGAGCAAGATCCGCGGCGCCTGA

Upstream 100 bases:

>100_bases
CGCCTCGATGCCCGCCACGGTGAATTCGTCCGTGTCCTGCGCGATCTTGCGCGACAATTCCAGTTCGATGCGATGAAGGA
AATTCTCAGGAAAATGCGCG

Downstream 100 bases:

>100_bases
AAGCGCCCCGGATCAGCCATCATTGTCCCATTTCAGGCCGCCAGTGCGGCCGTCACGATGGTGCCCGCCTCGGCCACCAG
TTGCTGCAGGTGCGCCTCGC

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MPPVDRNISDIVLIVDDVPENLSLLHDALDEAGYTVLVATHGEAALQRARQSLPDVILLDALMPGMDGFEVARRLKADFS
TQHIPIVFMTGLTETEHVVAAFAAGGADYVTKPIRPAEVLARIAAHMMNARQMKQARSALDAFGQATVAVRAADSRIVWQ
TPLARGLLNSYFANPETVAPEELLTWITAALRARADGREPAPLLIAEGNKRLLASFHDQTGDDEWMVVLREENDASAVES
LIAAFRLTQREAEVLYWVVQGKTSNDIGDILGNSPRTVNKHLEHVFEKLGVETRTAAANLAMSKIRGA

Sequences:

>Translated_308_residues
MPPVDRNISDIVLIVDDVPENLSLLHDALDEAGYTVLVATHGEAALQRARQSLPDVILLDALMPGMDGFEVARRLKADFS
TQHIPIVFMTGLTETEHVVAAFAAGGADYVTKPIRPAEVLARIAAHMMNARQMKQARSALDAFGQATVAVRAADSRIVWQ
TPLARGLLNSYFANPETVAPEELLTWITAALRARADGREPAPLLIAEGNKRLLASFHDQTGDDEWMVVLREENDASAVES
LIAAFRLTQREAEVLYWVVQGKTSNDIGDILGNSPRTVNKHLEHVFEKLGVETRTAAANLAMSKIRGA
>Mature_307_residues
PPVDRNISDIVLIVDDVPENLSLLHDALDEAGYTVLVATHGEAALQRARQSLPDVILLDALMPGMDGFEVARRLKADFST
QHIPIVFMTGLTETEHVVAAFAAGGADYVTKPIRPAEVLARIAAHMMNARQMKQARSALDAFGQATVAVRAADSRIVWQT
PLARGLLNSYFANPETVAPEELLTWITAALRARADGREPAPLLIAEGNKRLLASFHDQTGDDEWMVVLREENDASAVESL
IAAFRLTQREAEVLYWVVQGKTSNDIGDILGNSPRTVNKHLEHVFEKLGVETRTAAANLAMSKIRGA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=133, Percent_Identity=36.8421052631579, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1786599, Length=179, Percent_Identity=28.4916201117318, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790860, Length=137, Percent_Identity=31.3868613138686, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1786784, Length=112, Percent_Identity=33.9285714285714, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87082012, Length=145, Percent_Identity=27.5862068965517, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1790437, Length=103, Percent_Identity=33.9805825242718, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790299, Length=128, Percent_Identity=30.46875, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789809, Length=114, Percent_Identity=31.5789473684211, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788550, Length=103, Percent_Identity=33.9805825242718, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 33590; Mature: 33459

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPVDRNISDIVLIVDDVPENLSLLHDALDEAGYTVLVATHGEAALQRARQSLPDVILLD
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCHHHHHH
ALMPGMDGFEVARRLKADFSTQHIPIVFMTGLTETEHVVAAFAAGGADYVTKPIRPAEVL
HHCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHCCCCCHHHHH
ARIAAHMMNARQMKQARSALDAFGQATVAVRAADSRIVWQTPLARGLLNSYFANPETVAP
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECHHHHHHHHHHCCCCCCCCH
EELLTWITAALRARADGREPAPLLIAEGNKRLLASFHDQTGDDEWMVVLREENDASAVES
HHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHH
LIAAFRLTQREAEVLYWVVQGKTSNDIGDILGNSPRTVNKHLEHVFEKLGVETRTAAANL
HHHHHHHHHHCCEEEEEEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
AMSKIRGA
HHHHHCCC
>Mature Secondary Structure 
PPVDRNISDIVLIVDDVPENLSLLHDALDEAGYTVLVATHGEAALQRARQSLPDVILLD
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCHHHHHH
ALMPGMDGFEVARRLKADFSTQHIPIVFMTGLTETEHVVAAFAAGGADYVTKPIRPAEVL
HHCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHCCCCCHHHHH
ARIAAHMMNARQMKQARSALDAFGQATVAVRAADSRIVWQTPLARGLLNSYFANPETVAP
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECHHHHHHHHHHCCCCCCCCH
EELLTWITAALRARADGREPAPLLIAEGNKRLLASFHDQTGDDEWMVVLREENDASAVES
HHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHH
LIAAFRLTQREAEVLYWVVQGKTSNDIGDILGNSPRTVNKHLEHVFEKLGVETRTAAANL
HHHHHHHHHHCCEEEEEEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
AMSKIRGA
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA