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The map label for this gene is cynS

Identifier: 71907074

GI number: 71907074

Start: 1568913

End: 1569356

Strand: Reverse

Name: cynS

Synonym: Daro_1442

Alternate gene names: 71907074

Gene position: 1569356-1568913 (Counterclockwise)

Preceding gene: 71907075

Following gene: 71907069

Centisome position: 34.87

GC content: 60.14

Gene sequence:

>444_bases
ATGAACCGCGAAGAAGTGACCGATCTGATCGTCACCCAAAAAGTCAAAAAGAAACTGACCTGGGCCCAACTGGCCGAAGT
GGTCGGCCACAGCAAGGAATGGAGCACCGCCGCCCTGCTCGGCCAGATGACGCTGACCGAAGCCCAGGCCAAAGCCATCG
GCACCACCCTCGACCTGCCGGAAGAGGCCGTCACGCAACTGCAGGTCGTTCCCTACAAGGGTTCGCTGCCCACCGCCGTG
CCGACTGACCCGCTGATCTACCGCTTCTATGAACTGATCAGCGTTTACGGCACCACGTTCAAGTCGCTGATCCACGAAGA
GTTCGGCGACGGCATCATGAGCGCCATCGACTTCAACATGGACCTGACCCGCGAAGCCGACCCCAAGGGCGACCGCGTCA
GGATCACCATGAGCGGCAAGTTCCTGCCCTACAAGACCTACTGA

Upstream 100 bases:

>100_bases
AAGGACGAGCCGGAAGCGAAGTGATCCGGATCGGTCTGTCAGTCGCAAACGAACGGGGGCCTCGGCCCCCGTTTTTCCAG
CCAATCGAAAGGAATGCACC

Downstream 100 bases:

>100_bases
GCAACGCCCCAGCGAGTGAAATTTCGCCCGATTTTCCGGAAAGCCCCAGGATCCGCAGCTGCTTCCAGGGTCGCGGAAAT
CGGGCCTTTAGTTGGCTGAT

Product: cyanate hydratase

Products: NA

Alternate protein names: Cyanase; Cyanate hydrolase; Cyanate lyase

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MNREEVTDLIVTQKVKKKLTWAQLAEVVGHSKEWSTAALLGQMTLTEAQAKAIGTTLDLPEEAVTQLQVVPYKGSLPTAV
PTDPLIYRFYELISVYGTTFKSLIHEEFGDGIMSAIDFNMDLTREADPKGDRVRITMSGKFLPYKTY

Sequences:

>Translated_147_residues
MNREEVTDLIVTQKVKKKLTWAQLAEVVGHSKEWSTAALLGQMTLTEAQAKAIGTTLDLPEEAVTQLQVVPYKGSLPTAV
PTDPLIYRFYELISVYGTTFKSLIHEEFGDGIMSAIDFNMDLTREADPKGDRVRITMSGKFLPYKTY
>Mature_147_residues
MNREEVTDLIVTQKVKKKLTWAQLAEVVGHSKEWSTAALLGQMTLTEAQAKAIGTTLDLPEEAVTQLQVVPYKGSLPTAV
PTDPLIYRFYELISVYGTTFKSLIHEEFGDGIMSAIDFNMDLTREADPKGDRVRITMSGKFLPYKTY

Specific function: Catalyzes the reaction of cyanate with bicarbonate to produce ammonia and carbon dioxide

COG id: COG1513

COG function: function code P; Cyanate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cyanase family

Homologues:

Organism=Escherichia coli, GI1786535, Length=146, Percent_Identity=46.5753424657534, Blast_Score=150, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYNS_DECAR (Q47G40)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_284661.1
- HSSP:   P00816
- ProteinModelPortal:   Q47G40
- SMR:   Q47G40
- STRING:   Q47G40
- GeneID:   3569049
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_1442
- NMPDR:   fig|159087.4.peg.1329
- eggNOG:   COG1513
- HOGENOM:   HBG317643
- OMA:   KEADPKG
- ProtClustDB:   PRK02866
- BioCyc:   DARO159087:DARO_1442-MONOMER
- HAMAP:   MF_00535
- InterPro:   IPR008076
- InterPro:   IPR003712
- InterPro:   IPR010982
- Gene3D:   G3DSA:3.30.1160.10
- PIRSF:   PIRSF001263
- PRINTS:   PR01693
- TIGRFAMs:   TIGR00673

Pfam domain/function: PF02560 Cyanate_lyase; SSF55234 Cyanate_lyase_C; SSF47413 Lambda_like_DNA

EC number: =4.2.1.104

Molecular weight: Translated: 16460; Mature: 16460

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: ACT_SITE 88-88 ACT_SITE 91-91 ACT_SITE 114-114

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNREEVTDLIVTQKVKKKLTWAQLAEVVGHSKEWSTAALLGQMTLTEAQAKAIGTTLDLP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
EEAVTQLQVVPYKGSLPTAVPTDPLIYRFYELISVYGTTFKSLIHEEFGDGIMSAIDFNM
HHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCC
DLTREADPKGDRVRITMSGKFLPYKTY
CCCCCCCCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure
MNREEVTDLIVTQKVKKKLTWAQLAEVVGHSKEWSTAALLGQMTLTEAQAKAIGTTLDLP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
EEAVTQLQVVPYKGSLPTAVPTDPLIYRFYELISVYGTTFKSLIHEEFGDGIMSAIDFNM
HHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCC
DLTREADPKGDRVRITMSGKFLPYKTY
CCCCCCCCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA