Click here to switch to the map view.

The map label for this gene is braD [H]

Identifier: 71907069

GI number: 71907069

Start: 1562296

End: 1563873

Strand: Reverse

Name: braD [H]

Synonym: Daro_1437

Alternate gene names: 71907069

Gene position: 1563873-1562296 (Counterclockwise)

Preceding gene: 71907074

Following gene: 71907068

Centisome position: 34.74

GC content: 65.65

Gene sequence:

>1578_bases
ATGAAAAAAACTTTAATTGCTTTATTGCTCAGCCTCGGCAGCCTGTCGGCGCAAGCGGCGCTCGACCCGGCGCAGGTGCG
CCAGCTGGCCGCCGAGGACTCCAGCGACAAGGTGGCGGCCATCCGCCAACTGACGCAAACCGCCGACCCGGATGCCGTGC
GCGTCCTCAAGGCGATGGCCGACGACAGCCTGTTTCTGGCCGGCGACAAGGTCGTCATCATCGATGGCGACAAGGCCTTC
GACGGCGCGACCGGCGAAGCGCTCAAGATGCCGGCCAACCCCGAGTCGCCGACGGTCAATAACCGCATCCGCGGCGAACT
GGCCAGCGCGCTGGCCGCGCTGAAGTTGTTCGATGCCGATGCCACGGTCCGCCTCGCCTCGGCCCAGAAGCTGCAAAGCC
AGGTGACACCGGAAATGGGGCCGCTGCTGGCGCGGGCGCTGGAAAAGGAAACCGACGCCCAGATCAAGGCTCTGCTCACC
CTCGCTTACGCCCAGGCCAATCTCGGCAACGCCGACTCGGCCGCCCGCCTGGCCGCCGTCAAGGCGCTAGCCGAAACCTC
CTCGCCCACCATCAAGAGCGTCCTGCTGCCGCTGACCGAAAAGGCTGGCGAGCCGGACGACAAGGTGCGCTACGAAGCGA
TCGCCGCGTTGAAGGCGATCGACGGCCGGCTGGCCATTGCAGAAAACGTCGGCCGCGCCTTTTCCGGTCTGTCGCTCGGC
AGCATCCTGCTCCTCGGCGCGCTCGGCCTAGCCATTACCTACGGCGTCATGGGCATTATCAACATGGCGCACGGCGAGCT
GCTGATGGTCGGCGCCTACGCCACCTACGGCATGCAGACGCTGTTCCGCGCCTACTTCCCGGACGCCATCGACTGGTATC
TGCCGGCGGCCATCCCGGTCGCCTTCTTCGCCGCCGCGCTGGTCGGCGTCGTCATGGAACGCACGGTCATCCGCTGGCTC
TACGGCCGCACGCTGGAAACCCTGCTCGCCACCTGGGGCCTGTCGCTGGTCCTGATCCAGTCGGCGCGCGTGCTGTTCGG
CGCACAGAACGTCGAAGTCTCGAACCCGAGCTGGATGTCGGGCGGTATCGAAATCATGCCCAACCTGATCCTGCCGTGGA
ACCGCATCATCATTGTCGGCTTTGCGATGTTCGTGCTCTTCCTTGTCTGGGTGCTGATGAACAAGACCCGGCTCGGCATG
TTCGTCCGCGCCGTGACGCAAAACCGCCCGATGGCGGGTTGTGTCGGCGTACCGACCTCACGCATCGACACCATGGCCTT
CGCGCTGGGCGCCGGCATCGCCGGGCTGGGCGGCGTCGCGCTCTCCCAAATCTCCAACGTCGGGCCGGACATGGGCCAGG
GCTACATCGTCGATTCCTTCATGGTCGTCGTCGTCGGCGGCGTCGGGCAACTCGCCGGTGCGGTCTGGGCGGCGCTCGGT
CTGGGCATCTTCTCAAAACTGCTCGAAGGCTGGGCCGGGGCGGTCATCGCCAAGATCAGCATCCTCGTCTGCATCATCAT
CTTCATCCAGAAGCGCCCGCAGGGCATCTTCGCCCTCAAGGGCCGCTTCGTCGAGTAA

Upstream 100 bases:

>100_bases
TCCCCTTCCCTTGCCCCCACTACGCAATTCATCGTATTTCTGATCCCCGGCCCGCCCCGTAATCTGTCGCTGCACTGCAC
AAATTGCGGACGGACCATTG

Downstream 100 bases:

>100_bases
GGCTGAACCATGCGCAAATCTCTCACCCTTCGTATCCTGTCCTCACTGGACAAGAAAAGCTGGCAGGCGCTGGGTTTCTT
CCTCGCCCTCGCGTTGGTCG

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MKKTLIALLLSLGSLSAQAALDPAQVRQLAAEDSSDKVAAIRQLTQTADPDAVRVLKAMADDSLFLAGDKVVIIDGDKAF
DGATGEALKMPANPESPTVNNRIRGELASALAALKLFDADATVRLASAQKLQSQVTPEMGPLLARALEKETDAQIKALLT
LAYAQANLGNADSAARLAAVKALAETSSPTIKSVLLPLTEKAGEPDDKVRYEAIAALKAIDGRLAIAENVGRAFSGLSLG
SILLLGALGLAITYGVMGIINMAHGELLMVGAYATYGMQTLFRAYFPDAIDWYLPAAIPVAFFAAALVGVVMERTVIRWL
YGRTLETLLATWGLSLVLIQSARVLFGAQNVEVSNPSWMSGGIEIMPNLILPWNRIIIVGFAMFVLFLVWVLMNKTRLGM
FVRAVTQNRPMAGCVGVPTSRIDTMAFALGAGIAGLGGVALSQISNVGPDMGQGYIVDSFMVVVVGGVGQLAGAVWAALG
LGIFSKLLEGWAGAVIAKISILVCIIIFIQKRPQGIFALKGRFVE

Sequences:

>Translated_525_residues
MKKTLIALLLSLGSLSAQAALDPAQVRQLAAEDSSDKVAAIRQLTQTADPDAVRVLKAMADDSLFLAGDKVVIIDGDKAF
DGATGEALKMPANPESPTVNNRIRGELASALAALKLFDADATVRLASAQKLQSQVTPEMGPLLARALEKETDAQIKALLT
LAYAQANLGNADSAARLAAVKALAETSSPTIKSVLLPLTEKAGEPDDKVRYEAIAALKAIDGRLAIAENVGRAFSGLSLG
SILLLGALGLAITYGVMGIINMAHGELLMVGAYATYGMQTLFRAYFPDAIDWYLPAAIPVAFFAAALVGVVMERTVIRWL
YGRTLETLLATWGLSLVLIQSARVLFGAQNVEVSNPSWMSGGIEIMPNLILPWNRIIIVGFAMFVLFLVWVLMNKTRLGM
FVRAVTQNRPMAGCVGVPTSRIDTMAFALGAGIAGLGGVALSQISNVGPDMGQGYIVDSFMVVVVGGVGQLAGAVWAALG
LGIFSKLLEGWAGAVIAKISILVCIIIFIQKRPQGIFALKGRFVE
>Mature_525_residues
MKKTLIALLLSLGSLSAQAALDPAQVRQLAAEDSSDKVAAIRQLTQTADPDAVRVLKAMADDSLFLAGDKVVIIDGDKAF
DGATGEALKMPANPESPTVNNRIRGELASALAALKLFDADATVRLASAQKLQSQVTPEMGPLLARALEKETDAQIKALLT
LAYAQANLGNADSAARLAAVKALAETSSPTIKSVLLPLTEKAGEPDDKVRYEAIAALKAIDGRLAIAENVGRAFSGLSLG
SILLLGALGLAITYGVMGIINMAHGELLMVGAYATYGMQTLFRAYFPDAIDWYLPAAIPVAFFAAALVGVVMERTVIRWL
YGRTLETLLATWGLSLVLIQSARVLFGAQNVEVSNPSWMSGGIEIMPNLILPWNRIIIVGFAMFVLFLVWVLMNKTRLGM
FVRAVTQNRPMAGCVGVPTSRIDTMAFALGAGIAGLGGVALSQISNVGPDMGQGYIVDSFMVVVVGGVGQLAGAVWAALG
LGIFSKLLEGWAGAVIAKISILVCIIIFIQKRPQGIFALKGRFVE

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=295, Percent_Identity=30.1694915254237, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 55382; Mature: 55382

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTLIALLLSLGSLSAQAALDPAQVRQLAAEDSSDKVAAIRQLTQTADPDAVRVLKAMA
CHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHC
DDSLFLAGDKVVIIDGDKAFDGATGEALKMPANPESPTVNNRIRGELASALAALKLFDAD
CCCEEEECCEEEEEECCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
ATVRLASAQKLQSQVTPEMGPLLARALEKETDAQIKALLTLAYAQANLGNADSAARLAAV
CCEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
KALAETSSPTIKSVLLPLTEKAGEPDDKVRYEAIAALKAIDGRLAIAENVGRAFSGLSLG
HHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHH
SILLLGALGLAITYGVMGIINMAHGELLMVGAYATYGMQTLFRAYFPDAIDWYLPAAIPV
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
AFFAAALVGVVMERTVIRWLYGRTLETLLATWGLSLVLIQSARVLFGAQNVEVSNPSWMS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
GGIEIMPNLILPWNRIIIVGFAMFVLFLVWVLMNKTRLGMFVRAVTQNRPMAGCVGVPTS
CCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHH
RIDTMAFALGAGIAGLGGVALSQISNVGPDMGQGYIVDSFMVVVVGGVGQLAGAVWAALG
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHH
LGIFSKLLEGWAGAVIAKISILVCIIIFIQKRPQGIFALKGRFVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
>Mature Secondary Structure
MKKTLIALLLSLGSLSAQAALDPAQVRQLAAEDSSDKVAAIRQLTQTADPDAVRVLKAMA
CHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHC
DDSLFLAGDKVVIIDGDKAFDGATGEALKMPANPESPTVNNRIRGELASALAALKLFDAD
CCCEEEECCEEEEEECCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
ATVRLASAQKLQSQVTPEMGPLLARALEKETDAQIKALLTLAYAQANLGNADSAARLAAV
CCEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
KALAETSSPTIKSVLLPLTEKAGEPDDKVRYEAIAALKAIDGRLAIAENVGRAFSGLSLG
HHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHH
SILLLGALGLAITYGVMGIINMAHGELLMVGAYATYGMQTLFRAYFPDAIDWYLPAAIPV
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
AFFAAALVGVVMERTVIRWLYGRTLETLLATWGLSLVLIQSARVLFGAQNVEVSNPSWMS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
GGIEIMPNLILPWNRIIIVGFAMFVLFLVWVLMNKTRLGMFVRAVTQNRPMAGCVGVPTS
CCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHH
RIDTMAFALGAGIAGLGGVALSQISNVGPDMGQGYIVDSFMVVVVGGVGQLAGAVWAALG
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHH
LGIFSKLLEGWAGAVIAKISILVCIIIFIQKRPQGIFALKGRFVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]