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The map label for this gene is gph2 [H]

Identifier: 71906865

GI number: 71906865

Start: 1331293

End: 1331961

Strand: Reverse

Name: gph2 [H]

Synonym: Daro_1226

Alternate gene names: 71906865

Gene position: 1331961-1331293 (Counterclockwise)

Preceding gene: 71906866

Following gene: 71906862

Centisome position: 29.59

GC content: 60.84

Gene sequence:

>669_bases
ATGTTTGATGCTGTTCTGTTCGACCTGGATGGCACCTTGGCCGACACGGCGCCCGACCTGGGCGAGTCGGTCAACATTCT
GCTGCTGGAGGAAGGACGACTGCAGATCCCGCTTGAAACCCTGCGCCCCTTCACGTCGCAGGGCGTTCGTGGCCTGCTAA
AGACTGGCTTTGGCATCGACGTTGCGCATTCCGCCTACGAACGCCTGTCGCAACGCTTTCTCGCCATTTACGCAGAGCGC
TTGTGCCATGCCACCCGGCTGTTCGATGAAATTCCGGAATTGCTGGATGCGCTGGAGCAGATGAGTCTGGCCTGGGGGAT
CGTCACCAACAAACGGATGCGCTTTACCGACCCTTTAGTCGAATTGCTGCAACTCACCCCTCGTACCACCTGCGTCGTCA
GCGGCGACACGACGGCCGAAGTCAAGCCGTCGCCATTGCCCATCCTGCATGCCTGCCGCCTGCTGAACTGCCGGCCGGAG
CGCACACTCTACGTTGGCGATGACCGGCGTGACATCGTCGCCGGCAACGCAGCCGGTTGCAAGACAGTGGCTGTTTCCTA
TGGCTACCTCGGCGATAGCGGTCCTCCACACACTTGGGGCGCCGATCTGATCATCGACCGGCCAAGCGAACTGGCGGAAT
ACCTCGCTAGACACGCCGGGCCGAAATAA

Upstream 100 bases:

>100_bases
GAACCTGAAGAACTTGTCGGCATGAGCTACAACCCACTGACCAAGAAGTACTCACTCGGCAAAGACACCAGCGTCAATTA
CCTGATGCGCACCATCCGGC

Downstream 100 bases:

>100_bases
GCAGGTTGCGCGGCGTTAATTTTCGTTCGCAAAAACTGCCCAAAGCGACTGCGTAGCCTACCTTTTCGAGATATACCGAC
AAATCGAGAACCAGCCAGAC

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP 2; PGPase2 [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MFDAVLFDLDGTLADTAPDLGESVNILLLEEGRLQIPLETLRPFTSQGVRGLLKTGFGIDVAHSAYERLSQRFLAIYAER
LCHATRLFDEIPELLDALEQMSLAWGIVTNKRMRFTDPLVELLQLTPRTTCVVSGDTTAEVKPSPLPILHACRLLNCRPE
RTLYVGDDRRDIVAGNAAGCKTVAVSYGYLGDSGPPHTWGADLIIDRPSELAEYLARHAGPK

Sequences:

>Translated_222_residues
MFDAVLFDLDGTLADTAPDLGESVNILLLEEGRLQIPLETLRPFTSQGVRGLLKTGFGIDVAHSAYERLSQRFLAIYAER
LCHATRLFDEIPELLDALEQMSLAWGIVTNKRMRFTDPLVELLQLTPRTTCVVSGDTTAEVKPSPLPILHACRLLNCRPE
RTLYVGDDRRDIVAGNAAGCKTVAVSYGYLGDSGPPHTWGADLIIDRPSELAEYLARHAGPK
>Mature_222_residues
MFDAVLFDLDGTLADTAPDLGESVNILLLEEGRLQIPLETLRPFTSQGVRGLLKTGFGIDVAHSAYERLSQRFLAIYAER
LCHATRLFDEIPELLDALEQMSLAWGIVTNKRMRFTDPLVELLQLTPRTTCVVSGDTTAEVKPSPLPILHACRLLNCRPE
RTLYVGDDRRDIVAGNAAGCKTVAVSYGYLGDSGPPHTWGADLIIDRPSELAEYLARHAGPK

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=209, Percent_Identity=32.0574162679426, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 24431; Mature: 24431

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDAVLFDLDGTLADTAPDLGESVNILLLEEGRLQIPLETLRPFTSQGVRGLLKTGFGID
CCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCEEECHHHHCCHHHHHHHHHHHCCCCCH
VAHSAYERLSQRFLAIYAERLCHATRLFDEIPELLDALEQMSLAWGIVTNKRMRFTDPLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHH
ELLQLTPRTTCVVSGDTTAEVKPSPLPILHACRLLNCRPERTLYVGDDRRDIVAGNAAGC
HHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCEEEECCCCCC
KTVAVSYGYLGDSGPPHTWGADLIIDRPSELAEYLARHAGPK
EEEEEEECCCCCCCCCCCCCCCEEEECHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFDAVLFDLDGTLADTAPDLGESVNILLLEEGRLQIPLETLRPFTSQGVRGLLKTGFGID
CCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCEEECHHHHCCHHHHHHHHHHHCCCCCH
VAHSAYERLSQRFLAIYAERLCHATRLFDEIPELLDALEQMSLAWGIVTNKRMRFTDPLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHH
ELLQLTPRTTCVVSGDTTAEVKPSPLPILHACRLLNCRPERTLYVGDDRRDIVAGNAAGC
HHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCEEEECCCCCC
KTVAVSYGYLGDSGPPHTWGADLIIDRPSELAEYLARHAGPK
EEEEEEECCCCCCCCCCCCCCCEEEECHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]