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The map label for this gene is nemA [H]

Identifier: 71906738

GI number: 71906738

Start: 1201948

End: 1203030

Strand: Reverse

Name: nemA [H]

Synonym: Daro_1099

Alternate gene names: 71906738

Gene position: 1203030-1201948 (Counterclockwise)

Preceding gene: 71906739

Following gene: 71906737

Centisome position: 26.73

GC content: 66.94

Gene sequence:

>1083_bases
ATGACTACCTTGTTCGATCCAATCCAGATGGGCGACATCGCCCTTGCCAACCGTATCGTGATGGCTCCGCTGACGCGCAA
CCGCGCCATCGAGGGCAACAAGCCCGGCCCGCTGACCGTCGAGTACTACCGCCAGCGCGCCACGGCCGGCCTGATCGTCG
CCGAAGCCAGCCCGATCAGCCCGACCGCCCAGGGGTACCTCGACACCCCGGGCATCTGGTCGGCCGAGCAGGTCGTGGCC
TGGCAGGCCGTAACCAAGGCCGTGCATGCCGAAGGCGGCAAGATCGTGCTGCAGCTCTGGCACGTCGGCCGCATTTCGCA
CATCTCGCTGCTGCCGGCTGGCGAAGTGCCGGTGTCGTCCACCGACAAGGTGGCCGATGCATCGACCTTTACCAGCGAAG
GCTTCATCCCCGTGTCCAAGCCGCGCGCCCTGCACGACGACGAAATCCCGGCGCTGATCGAGGACTACCGCAAGGCCGCC
CGCAATGCGATCGCCGCCGGCTTCGACGGTGTCGAGATCCACGCCGCCAACACCTACCTGATCGAGCAGTTCCTGCGCGA
CAGCGTCAATGACCGCAGCGGCCCGTACGGCGGCAGCATCGAGAACCGCACCCGCCTGTTGCTCGAAGTCACCCGCGCTG
TTGCCGGCGAAATCGGTGCTGGCCGCACCGGCATCCGCCTGAGCCCGATGACCACCTTCACGGCACCGCTCGACAGCAAC
CCGCAGGCGCTCTACGGCTATGTCGTCGAGCAACTGGCCCCGCTCGGCCTGGCTTACCTGCATGTAATCGAAGGCGAAAC
CGGCGGCACCCGCACGCCGGAGGGCAAGCCTTTCGACTACGCGGCCCTGCACCGCGCCTACCCCGGCGTCTGGATGCTCA
ATAATGGCTACGACCGCGACCTGGCGCTGCAAACCGTCGCCGACGGCAAGGCCGACCTGATCGCCTTTGGCCGTCCGTTC
ATCAGCACGCCCGACCTCGTCCGCCGTCTGCGCGACGGTGCGCCGTTGAACGAATTGCGCGCCGACAAGCTCTATGGTGG
TGGCGCCGAGGGTTACACCGACTACCCGACGCTGGCCGGCTAA

Upstream 100 bases:

>100_bases
GCACATGCAAACGTCAATGGCCGCCTCCATAGCGGCCATTTTTTGAACCCTATCACTAGACGACTGGTCTATTTAGCCAA
CCACTTCAGAGGAGCACCGT

Downstream 100 bases:

>100_bases
CAACAACAAACCCCAACCCCAGGCCGCGCACCGGAAAATCCGGGCGCGGCCCACAGAGAACCGCATGAATTCCTACCGCC
GCTGGCTTCCAGCCATCGCC

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MTTLFDPIQMGDIALANRIVMAPLTRNRAIEGNKPGPLTVEYYRQRATAGLIVAEASPISPTAQGYLDTPGIWSAEQVVA
WQAVTKAVHAEGGKIVLQLWHVGRISHISLLPAGEVPVSSTDKVADASTFTSEGFIPVSKPRALHDDEIPALIEDYRKAA
RNAIAAGFDGVEIHAANTYLIEQFLRDSVNDRSGPYGGSIENRTRLLLEVTRAVAGEIGAGRTGIRLSPMTTFTAPLDSN
PQALYGYVVEQLAPLGLAYLHVIEGETGGTRTPEGKPFDYAALHRAYPGVWMLNNGYDRDLALQTVADGKADLIAFGRPF
ISTPDLVRRLRDGAPLNELRADKLYGGGAEGYTDYPTLAG

Sequences:

>Translated_360_residues
MTTLFDPIQMGDIALANRIVMAPLTRNRAIEGNKPGPLTVEYYRQRATAGLIVAEASPISPTAQGYLDTPGIWSAEQVVA
WQAVTKAVHAEGGKIVLQLWHVGRISHISLLPAGEVPVSSTDKVADASTFTSEGFIPVSKPRALHDDEIPALIEDYRKAA
RNAIAAGFDGVEIHAANTYLIEQFLRDSVNDRSGPYGGSIENRTRLLLEVTRAVAGEIGAGRTGIRLSPMTTFTAPLDSN
PQALYGYVVEQLAPLGLAYLHVIEGETGGTRTPEGKPFDYAALHRAYPGVWMLNNGYDRDLALQTVADGKADLIAFGRPF
ISTPDLVRRLRDGAPLNELRADKLYGGGAEGYTDYPTLAG
>Mature_359_residues
TTLFDPIQMGDIALANRIVMAPLTRNRAIEGNKPGPLTVEYYRQRATAGLIVAEASPISPTAQGYLDTPGIWSAEQVVAW
QAVTKAVHAEGGKIVLQLWHVGRISHISLLPAGEVPVSSTDKVADASTFTSEGFIPVSKPRALHDDEIPALIEDYRKAAR
NAIAAGFDGVEIHAANTYLIEQFLRDSVNDRSGPYGGSIENRTRLLLEVTRAVAGEIGAGRTGIRLSPMTTFTAPLDSNP
QALYGYVVEQLAPLGLAYLHVIEGETGGTRTPEGKPFDYAALHRAYPGVWMLNNGYDRDLALQTVADGKADLIAFGRPFI
STPDLVRRLRDGAPLNELRADKLYGGGAEGYTDYPTLAG

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=365, Percent_Identity=47.6712328767123, Blast_Score=307, Evalue=7e-85,
Organism=Escherichia coli, GI1789463, Length=361, Percent_Identity=29.0858725761773, Blast_Score=105, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17559802, Length=266, Percent_Identity=29.6992481203008, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17559804, Length=272, Percent_Identity=27.2058823529412, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17565138, Length=269, Percent_Identity=29.368029739777, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17564188, Length=137, Percent_Identity=33.5766423357664, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI72001454, Length=277, Percent_Identity=27.0758122743682, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17540738, Length=134, Percent_Identity=34.3283582089552, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6325086, Length=375, Percent_Identity=40, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6321973, Length=375, Percent_Identity=38.4, Blast_Score=213, Evalue=4e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 38752; Mature: 38621

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLFDPIQMGDIALANRIVMAPLTRNRAIEGNKPGPLTVEYYRQRATAGLIVAEASPIS
CCCCCCCHHHCCHHHHCEEEEEECCCCCCCCCCCCCCEEHHHHHHHCCCEEEEECCCCCC
PTAQGYLDTPGIWSAEQVVAWQAVTKAVHAEGGKIVLQLWHVGRISHISLLPAGEVPVSS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCCCC
TDKVADASTFTSEGFIPVSKPRALHDDEIPALIEDYRKAARNAIAAGFDGVEIHAANTYL
CCCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECHHHH
IEQFLRDSVNDRSGPYGGSIENRTRLLLEVTRAVAGEIGAGRTGIRLSPMTTFTAPLDSN
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCEEEECCCCCC
PQALYGYVVEQLAPLGLAYLHVIEGETGGTRTPEGKPFDYAALHRAYPGVWMLNNGYDRD
CHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCC
LALQTVADGKADLIAFGRPFISTPDLVRRLRDGAPLNELRADKLYGGGAEGYTDYPTLAG
EEEEECCCCCEEEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTLFDPIQMGDIALANRIVMAPLTRNRAIEGNKPGPLTVEYYRQRATAGLIVAEASPIS
CCCCCCHHHCCHHHHCEEEEEECCCCCCCCCCCCCCEEHHHHHHHCCCEEEEECCCCCC
PTAQGYLDTPGIWSAEQVVAWQAVTKAVHAEGGKIVLQLWHVGRISHISLLPAGEVPVSS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCCCC
TDKVADASTFTSEGFIPVSKPRALHDDEIPALIEDYRKAARNAIAAGFDGVEIHAANTYL
CCCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECHHHH
IEQFLRDSVNDRSGPYGGSIENRTRLLLEVTRAVAGEIGAGRTGIRLSPMTTFTAPLDSN
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCEEEECCCCCC
PQALYGYVVEQLAPLGLAYLHVIEGETGGTRTPEGKPFDYAALHRAYPGVWMLNNGYDRD
CHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCC
LALQTVADGKADLIAFGRPFISTPDLVRRLRDGAPLNELRADKLYGGGAEGYTDYPTLAG
EEEEECCCCCEEEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]