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The map label for this gene is nemR [H]

Identifier: 71906739

GI number: 71906739

Start: 1203131

End: 1203715

Strand: Reverse

Name: nemR [H]

Synonym: Daro_1100

Alternate gene names: 71906739

Gene position: 1203715-1203131 (Counterclockwise)

Preceding gene: 71906740

Following gene: 71906738

Centisome position: 26.74

GC content: 62.39

Gene sequence:

>585_bases
ATGAACAATCGTCACGACAATACCCGCCAGCACATTCTCGAAACCGGCCACCGCATCATCGCGGGCAAGGGCTTCGCGAG
CGTCGGGCTGAACGAAATCCTCACTTCGGCCGGCGTGCCCAAGGGTTCGTTCTACCACTACTTCGAATCCAAGGAACAAT
ACGGCCAGGCTTTGCTGCAGGAGTATTTCGACCATTACCTGGCCGATCTCGACGAACTGTTCGCCGCCGATGCCGCCTCC
GGGCACGAGCGCCTGATACGCTACTGGCAGCGCTGGCTGGGCAAGCAACTCGAGGCCTGTGCCGAGCAGAAATGTCTGGT
CGTCAAGCTGGCCGGCGAAGTGTCCGACCTCTCCGATGCCATGCGCATCACCCTGCGCGACGGCACGGACCAGATCGTCA
AGCGCATCGCCCGCATCATCGAAGCCGGTGTTGCCGATGGCTCGGTGCCGCCGCTCGACCCGCAGATGACCGCCCAGACC
CTGTACCAGATGTGGCTGGGCGCCAGCCTGCTTGGCAAGTTGCACCGCGACACCGCGGCGCTGGAAAACGCCATGGTCTT
CACCCGAAAACTGCTGTCCCGCTAA

Upstream 100 bases:

>100_bases
TGAGAAAACTCACGGCGAGGATTTCCGGGCAGACAATACCGTTCATGGACGGAAAAATACCTACTAGACGACCGGTCTAA
TTTGTGACAGACTGCCCTTC

Downstream 100 bases:

>100_bases
GCACATGCAAACGTCAATGGCCGCCTCCATAGCGGCCATTTTTTGAACCCTATCACTAGACGACTGGTCTATTTAGCCAA
CCACTTCAGAGGAGCACCGT

Product: TetR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 194; Mature: 194

Protein sequence:

>194_residues
MNNRHDNTRQHILETGHRIIAGKGFASVGLNEILTSAGVPKGSFYHYFESKEQYGQALLQEYFDHYLADLDELFAADAAS
GHERLIRYWQRWLGKQLEACAEQKCLVVKLAGEVSDLSDAMRITLRDGTDQIVKRIARIIEAGVADGSVPPLDPQMTAQT
LYQMWLGASLLGKLHRDTAALENAMVFTRKLLSR

Sequences:

>Translated_194_residues
MNNRHDNTRQHILETGHRIIAGKGFASVGLNEILTSAGVPKGSFYHYFESKEQYGQALLQEYFDHYLADLDELFAADAAS
GHERLIRYWQRWLGKQLEACAEQKCLVVKLAGEVSDLSDAMRITLRDGTDQIVKRIARIIEAGVADGSVPPLDPQMTAQT
LYQMWLGASLLGKLHRDTAALENAMVFTRKLLSR
>Mature_194_residues
MNNRHDNTRQHILETGHRIIAGKGFASVGLNEILTSAGVPKGSFYHYFESKEQYGQALLQEYFDHYLADLDELFAADAAS
GHERLIRYWQRWLGKQLEACAEQKCLVVKLAGEVSDLSDAMRITLRDGTDQIVKRIARIIEAGVADGSVPPLDPQMTAQT
LYQMWLGASLLGKLHRDTAALENAMVFTRKLLSR

Specific function: Represses the transcription of the nemRA operon by binding to the nemR box [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693141, Length=194, Percent_Identity=36.0824742268041, Blast_Score=136, Evalue=1e-33,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR001647 [H]

Pfam domain/function: PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 21776; Mature: 21776

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNRHDNTRQHILETGHRIIAGKGFASVGLNEILTSAGVPKGSFYHYFESKEQYGQALLQ
CCCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
EYFDHYLADLDELFAADAASGHERLIRYWQRWLGKQLEACAEQKCLVVKLAGEVSDLSDA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHH
MRITLRDGTDQIVKRIARIIEAGVADGSVPPLDPQMTAQTLYQMWLGASLLGKLHRDTAA
HHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LENAMVFTRKLLSR
HHHHHHHHHHHHCC
>Mature Secondary Structure
MNNRHDNTRQHILETGHRIIAGKGFASVGLNEILTSAGVPKGSFYHYFESKEQYGQALLQ
CCCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
EYFDHYLADLDELFAADAASGHERLIRYWQRWLGKQLEACAEQKCLVVKLAGEVSDLSDA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHH
MRITLRDGTDQIVKRIARIIEAGVADGSVPPLDPQMTAQTLYQMWLGASLLGKLHRDTAA
HHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LENAMVFTRKLLSR
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]