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The map label for this gene is ogt [H]

Identifier: 71906178

GI number: 71906178

Start: 596260

End: 596748

Strand: Reverse

Name: ogt [H]

Synonym: Daro_0538

Alternate gene names: 71906178

Gene position: 596748-596260 (Counterclockwise)

Preceding gene: 71906179

Following gene: 71906177

Centisome position: 13.26

GC content: 63.6

Gene sequence:

>489_bases
ATGCAAACTCGACCGAACCAGACCTATCAGGCCATCGTGGCCGCCCCCGGTTTCTGCCTGGGGGTGCAGTGTGACGATGA
TGAAATCACCGGCATCGACTTTCTCGAACCACGCCCGGAAATCGCCGCCAGTTCTCCGCTGGCCGCCGAAGCAGTGCGTC
AGCTCAAGGCCTACATCGCCGACCCGCATTTTGCCTTTGGCCTGCCCCTCAGACCAGCGGGCACGACCTTCCAGCGCCGT
GTCTGGGAACAGATTTCCGCCATCCCGACCGGCCAGACGCACACCTATGGCGAAGTTGCGAAGAACCTGAAGAATGCCCC
ACGGGCCGTCGGCCAGGCTTGCGGATCGAACCCTTACCCGGTCGTCGTCCCCTGCCACCGCGTCGTCGCCACTGGCGGCG
GACTGGGCGGTTTCTCCCGCGAACGGGGCGGTTTCCTGCTCGACGTCAAACGCTGGTTGCTGACGCATGAAAATAGCCTC
CCAGGCTGA

Upstream 100 bases:

>100_bases
TGGTCAATACCTACGAAGGGACGCACGACGTGCACGCCCTGATCCTTGGCCGGGCCCAAACCGGGATTTCGGCTTTCTAG
GCATCCTCGGAGGCTCCCCA

Downstream 100 bases:

>100_bases
CCTTTCCGAGATCGACAATTTCTGCGATGCCCTGTGGCTGGAAGACGGTCTGGCCAAGGCGACGCTGGACAGCTATCGCT
CCGATCTCAGCCGGCTGGCC

Product: methylated-DNA-(protein)-cysteine S-methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MQTRPNQTYQAIVAAPGFCLGVQCDDDEITGIDFLEPRPEIAASSPLAAEAVRQLKAYIADPHFAFGLPLRPAGTTFQRR
VWEQISAIPTGQTHTYGEVAKNLKNAPRAVGQACGSNPYPVVVPCHRVVATGGGLGGFSRERGGFLLDVKRWLLTHENSL
PG

Sequences:

>Translated_162_residues
MQTRPNQTYQAIVAAPGFCLGVQCDDDEITGIDFLEPRPEIAASSPLAAEAVRQLKAYIADPHFAFGLPLRPAGTTFQRR
VWEQISAIPTGQTHTYGEVAKNLKNAPRAVGQACGSNPYPVVVPCHRVVATGGGLGGFSRERGGFLLDVKRWLLTHENSL
PG
>Mature_162_residues
MQTRPNQTYQAIVAAPGFCLGVQCDDDEITGIDFLEPRPEIAASSPLAAEAVRQLKAYIADPHFAFGLPLRPAGTTFQRR
VWEQISAIPTGQTHTYGEVAKNLKNAPRAVGQACGSNPYPVVVPCHRVVATGGGLGGFSRERGGFLLDVKRWLLTHENSL
PG

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1787596, Length=110, Percent_Identity=43.6363636363636, Blast_Score=81, Evalue=4e-17,
Organism=Escherichia coli, GI1788542, Length=126, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320001, Length=68, Percent_Identity=44.1176470588235, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17439; Mature: 17439

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTRPNQTYQAIVAAPGFCLGVQCDDDEITGIDFLEPRPEIAASSPLAAEAVRQLKAYIA
CCCCCCCHHHHHEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
DPHFAFGLPLRPAGTTFQRRVWEQISAIPTGQTHTYGEVAKNLKNAPRAVGQACGSNPYP
CCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCE
VVVPCHRVVATGGGLGGFSRERGGFLLDVKRWLLTHENSLPG
EEEECHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQTRPNQTYQAIVAAPGFCLGVQCDDDEITGIDFLEPRPEIAASSPLAAEAVRQLKAYIA
CCCCCCCHHHHHEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
DPHFAFGLPLRPAGTTFQRRVWEQISAIPTGQTHTYGEVAKNLKNAPRAVGQACGSNPYP
CCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCE
VVVPCHRVVATGGGLGGFSRERGGFLLDVKRWLLTHENSLPG
EEEECHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]