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The map label for this gene is smf [H]

Identifier: 71905661

GI number: 71905661

Start: 24827

End: 25903

Strand: Reverse

Name: smf [H]

Synonym: Daro_0019

Alternate gene names: 71905661

Gene position: 25903-24827 (Counterclockwise)

Preceding gene: 71905662

Following gene: 161377478

Centisome position: 0.58

GC content: 61.47

Gene sequence:

>1077_bases
GTGAGCCATAACGACAGTCTGGCCGCCTGGTTGCGGCTGACCCTGATCCCGGGCATCGGCGGCGAGACGCAAAGAAAGCT
TCTCGCCGCTTTCGGTTTACCGGAAGCCATTTTCTCGGCTGGCCGCCTGGAAGCACGCGGCGTCATCGGTAACCGCGCCG
ATCTGCTGTTCGATTTTGATCCGACGGAAGCGGTAGCACACAGTCTCGAATGGGCCAGGCAACCGGGGCAACACATCATC
TCGCTGGCCGACGAAGCCTACCCGAAAGCACTGCTCGAAATAGCCGACCCGCCCAGCCTGCTCTACGTACGCGGCAACCT
AGCCCTGCTCCAGAAGCGCGGACTGGCCATGGTTGGTAGCCGCAATGCAACACCGCAAGGCGTGCAAACCGCCGAAAACT
TCGCCAAAACGCTGGCCGCCAAGGGTCTGACAATCATTAGCGGACTGGCACTGGGGATTGATGCCGCCGCCCACCGTGGC
GCCCTGGCTGCCAAGGGGGAAACCATCGCGGTGATCGGCACCGGGCCCGACCGCATCTACCCGGCACGCAACAAGGAGCT
GGCTTTGGCGATTGTCGAATCCGGTGCGATCGTTTCCGAATTCCCGCTCGGCACACCGGCCATCGCTTCAAATTTCCCAA
GGCGCAATCGGATCATTTCCGGACTATCGTGCGGCGTACTGGTGGTCGAAGCGGCGCCGGAAAGTGGCTCGCTGATCACG
GCGCGGCTTGCCGCAGAGCAGGGGCGTGAAGTTTTCGCCATTCCCGGCTCGATCCACTCACCAGTTGCTCGTGGTTGCCA
CAAATTGATCAAGCAGGGTGCCAAGCTGGTTGAAACCGCTACCGACATCCTGGAGGAGCTGGGCAGTTTCAACGCAGCTC
CCGCAGCAGACATCCCATCGGATAAGGCCGATGAAGGGCCGATTCTCACTGCACTTGGCCACGATCCATGCAGCCTTGAC
GACCTCGTCGAACGAACCACCATGAGCGCCGATCAGTTACTGCCGGAACTCCTGACACTGGAGCTTTGCGGCCTGATCGC
CACCCTGCCCGGTAACCGCTACCAGCGCCTGAACTAG

Upstream 100 bases:

>100_bases
ATGGCCTTGCCTTCGTTTTCCGCGTCTTTGACCGCGTCGCCTACGCCTTGGTCGTCGAGTCCTCCAAGGCAGTCATCATC
GGGGACTCCGCACTGAACCC

Downstream 100 bases:

>100_bases
GCACCTATATATATTGACGGCTTGCCAAGCCGCTCGCCGGGCACTTATCATGCCCCGGCACCCAACAGGCCAAACCATGT
CCAAAAAGCTGATCATTGCC

Product: SMF protein

Products: NA

Alternate protein names: DNA-processing chain A [H]

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MSHNDSLAAWLRLTLIPGIGGETQRKLLAAFGLPEAIFSAGRLEARGVIGNRADLLFDFDPTEAVAHSLEWARQPGQHII
SLADEAYPKALLEIADPPSLLYVRGNLALLQKRGLAMVGSRNATPQGVQTAENFAKTLAAKGLTIISGLALGIDAAAHRG
ALAAKGETIAVIGTGPDRIYPARNKELALAIVESGAIVSEFPLGTPAIASNFPRRNRIISGLSCGVLVVEAAPESGSLIT
ARLAAEQGREVFAIPGSIHSPVARGCHKLIKQGAKLVETATDILEELGSFNAAPAADIPSDKADEGPILTALGHDPCSLD
DLVERTTMSADQLLPELLTLELCGLIATLPGNRYQRLN

Sequences:

>Translated_358_residues
MSHNDSLAAWLRLTLIPGIGGETQRKLLAAFGLPEAIFSAGRLEARGVIGNRADLLFDFDPTEAVAHSLEWARQPGQHII
SLADEAYPKALLEIADPPSLLYVRGNLALLQKRGLAMVGSRNATPQGVQTAENFAKTLAAKGLTIISGLALGIDAAAHRG
ALAAKGETIAVIGTGPDRIYPARNKELALAIVESGAIVSEFPLGTPAIASNFPRRNRIISGLSCGVLVVEAAPESGSLIT
ARLAAEQGREVFAIPGSIHSPVARGCHKLIKQGAKLVETATDILEELGSFNAAPAADIPSDKADEGPILTALGHDPCSLD
DLVERTTMSADQLLPELLTLELCGLIATLPGNRYQRLN
>Mature_357_residues
SHNDSLAAWLRLTLIPGIGGETQRKLLAAFGLPEAIFSAGRLEARGVIGNRADLLFDFDPTEAVAHSLEWARQPGQHIIS
LADEAYPKALLEIADPPSLLYVRGNLALLQKRGLAMVGSRNATPQGVQTAENFAKTLAAKGLTIISGLALGIDAAAHRGA
LAAKGETIAVIGTGPDRIYPARNKELALAIVESGAIVSEFPLGTPAIASNFPRRNRIISGLSCGVLVVEAAPESGSLITA
RLAAEQGREVFAIPGSIHSPVARGCHKLIKQGAKLVETATDILEELGSFNAAPAADIPSDKADEGPILTALGHDPCSLDD
LVERTTMSADQLLPELLTLELCGLIATLPGNRYQRLN

Specific function: Essential for efficient chromosomal DNA transformation but not required for plasmid transformation [H]

COG id: COG0758

COG function: function code LU; Predicted Rossmann fold nucleotide-binding protein involved in DNA uptake

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smf family [H]

Homologues:

Organism=Escherichia coli, GI48994932, Length=359, Percent_Identity=43.7325905292479, Blast_Score=244, Evalue=8e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003488 [H]

Pfam domain/function: PF02481 DNA_processg_A [H]

EC number: NA

Molecular weight: Translated: 37581; Mature: 37449

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHNDSLAAWLRLTLIPGIGGETQRKLLAAFGLPEAIFSAGRLEARGVIGNRADLLFDFD
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHCCCCEECCCCCCCCCEEEECC
PTEAVAHSLEWARQPGQHIISLADEAYPKALLEIADPPSLLYVRGNLALLQKRGLAMVGS
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCEEEECC
RNATPQGVQTAENFAKTLAAKGLTIISGLALGIDAAAHRGALAAKGETIAVIGTGPDRIY
CCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEECCCEEEEEECCCCCCC
PARNKELALAIVESGAIVSEFPLGTPAIASNFPRRNRIISGLSCGVLVVEAAPESGSLIT
CCCCCEEEEEEEECCCEEECCCCCCCHHHHCCCHHHHHHCCCCCCEEEEEECCCCCCEEE
ARLAAEQGREVFAIPGSIHSPVARGCHKLIKQGAKLVETATDILEELGSFNAAPAADIPS
EEEHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DKADEGPILTALGHDPCSLDDLVERTTMSADQLLPELLTLELCGLIATLPGNRYQRLN
CCCCCCCEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SHNDSLAAWLRLTLIPGIGGETQRKLLAAFGLPEAIFSAGRLEARGVIGNRADLLFDFD
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHCCCCEECCCCCCCCCEEEECC
PTEAVAHSLEWARQPGQHIISLADEAYPKALLEIADPPSLLYVRGNLALLQKRGLAMVGS
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCEEEECC
RNATPQGVQTAENFAKTLAAKGLTIISGLALGIDAAAHRGALAAKGETIAVIGTGPDRIY
CCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEECCCEEEEEECCCCCCC
PARNKELALAIVESGAIVSEFPLGTPAIASNFPRRNRIISGLSCGVLVVEAAPESGSLIT
CCCCCEEEEEEEECCCEEECCCCCCCHHHHCCCHHHHHHCCCCCCEEEEEECCCCCCEEE
ARLAAEQGREVFAIPGSIHSPVARGCHKLIKQGAKLVETATDILEELGSFNAAPAADIPS
EEEHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DKADEGPILTALGHDPCSLDDLVERTTMSADQLLPELLTLELCGLIATLPGNRYQRLN
CCCCCCCEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7768823; 7542800 [H]