Click here to switch to the map view.

The map label for this gene is topB [H]

Identifier: 161377478

GI number: 161377478

Start: 22231

End: 24750

Strand: Reverse

Name: topB [H]

Synonym: Daro_0018

Alternate gene names: 161377478

Gene position: 24750-22231 (Counterclockwise)

Preceding gene: 71905661

Following gene: 71905657

Centisome position: 0.55

GC content: 60.63

Gene sequence:

>2520_bases
ATGTCCAAAAAGCTGATCATTGCCGAAAAACCTTCCGTCGCCGCCGACATCGCTCGGGCGCTGGGGGGCTTCACCAAACA
CGACGATTACTTCGAAAGCGAAGAGCTCGTGCTCTCCTCGGCCGTCGGTCACCTGCTGGAACTGGCCTGCCCCGAAGAGT
TCGAGGTCAAGCGCGGCAAATGGTCCTTCGCCCACCTTCCGGTCATTCCGCCACACTTCGCCCTGAAGCCGATCGAAAAG
ACGGAATCGCGCCTCAAGGTGCTCAACAAGCTGATCAAACGCAAGGACGTTACCGGCCTGATCAATGCCTGTGACGCGGG
GCGTGAAGGCGAGTTGATCTTCAATTACATCGCCCAGAACGCCAAGTCCGGCAAGCCGGTCCAACGCCTGTGGCTGCAGT
CGATGACCCAGGGCGCCATCCGCGACGGCTTCTCCCGCCTGCGTCCCGGGCAGGAAATGGAAGGCCTGGGCGATGCCGCC
GTCTGCCGCTCCGAATCCGACTGGCTGGTTGGCATCAACGGCACGCGGGCGATGACTGCCTTCAACTCGAAGACCGGCGG
CTTCCACCTGACCACCGTTGGTCGCGTGCAAACCCCAACGCTGGCCATCGTTGTCGAGCGTGAACGCAAGATTCGCGACT
TCAAATCACGCGACTACTGGGAAGTCGAAGCCGATTTCACGGCCAAGGCCGGCTCCTACAAGGGCAAATGGTTCGACGAA
GCCTTCAAGGGCAAAAACGACGACGAACATGCCCGCGCCGACCGCCTGTGGGAAGTTGCCCGCGCCGAAGCCATCCGCGC
CGCCACTGAAGGCAAGCCCGGTGAAGTGACCGAAGAAGCCAAGCCGGAAACCCGTTTGTCGCCGGCGCTCTTCGATCTGA
CCACCCTGCAGCGCGAAGCAAACGCTCGCTTCGGCTTCTCGGCCAAGACCACGCTATCGCTGGCCCAGGCCCTGTACGAA
AAACACAAGGTTCTGACCTATCCACGAACCGACTCCCGCTGCCTGCCAGAAGACTACCTGCCAACCGTCAAGGAAACCTT
GACCATCCTGACCGGCGAAGGTGCCGGCAAGGGGCACGACGAAGTTCTGCTCGCCCGCTATTCACCGTTTGCCCACCAGA
TCCTGGCGCGCAACTGGGTGCTACCCAACAAGCGCATCTTCAATAACGCCAAGATCAGCGATCACTTCGCCATCATCCCG
ACGCCGCAGGCGCCCAAGAGCCTGAACGAAATGGAACAGAAGCTGTACGACTTCGTCGTTCGCCGCTTCCTGTCCGTCTT
CTTCCCGGCCGCCGAATACATGGTCACCACCCGTATCACCCGGGTCGAAGGCCACCCCTTCAAGACTGAGGGCAAGGTGC
TGGTCAATCCGGGCTGGCTCGCCGTCTACGGCAAGGAAGGCCAGGACGGCGACGAAGGTAATCTGGTTGCCGTCGATGCC
AAGGAAAAGGTCAAGACCGACGAAGTCACCGTCAAGGCCAACGCCACCAAGCCGCCACCACGCTACTCGGAAGCCACCCT
GCTCTCCGCCATGGAAGGTGCCGGCAAGATGGTCGACGACGAAGAACTGAAGGCGGCCATGGCCGGCCGCGGCCTAGGCA
CCCCGGCCACCCGCGCCCAGATCATCGAGAACCTGATCGGCGAGCAATACATGCATCGCGAAGGCCGGGAACTGATCCCG
ACCGCCAAGGCATTTACCCTGATGACGCTGCTCAACGGCCTCGGCATCAAGGAATTGACCCAACCGGAACTGACCGGCGA
CTGGGAATGGAAGCTCGGCCGTATCGAGAAGGGCGAATTCACGCGCGACGAGTTCATGCGTGAAATCGCCGAAATGACCC
GTCACATGGTCGAACGCGCCAAGCAATACGAAGCCGACACCATCCCCGGCGACTTCGGTGTGCTGACGGCGCCCTGCCCC
AAGTGCGGCGGCCAGATTCGCGAAACCTACAAGAAGTTCCAGTGCGGCAGTTGCGACTACGCACTGTGGAAGATCGTCGC
CGGCCGTCAGTACGAACCGGCAGAAATCGAGCAATTGCTGACCGAAAAGCAGGTCGGCCCACTGACCGGTTTCCGCAACA
AGATGGGCCGCAGCTTTTCCGCCGCGATCAAGCTCAATGATGAATTGCAGCCGGAATTCGACTTCGGCCAGGACAAGGCG
GATGCCGCCGATGCAGAACCCGTCGATTTCTCCGCGCAGGAAAGCCTGGGCAAGTGCCCGAAGTGCGCGGCCAATGTCTT
CGAGCACGGCACTTCCTACGTCTGCGAAAAATCGGTCGGCCCGGCCAAGACCTGCGATTTCCGCTCCGGCAAGATCATCC
TGCAGCAGCCGATCGATGCCGCCCAGATGAAGAAGCTGCTGGCCGATGGCAAAACCGACCTGCTGAAGGAATTCGTGTCC
AACCGCACCCGCCGCAAGTTCTCGGCCTATCTGGTCAATCAGGGTGGCAAAGTCGGATTCGAGTTTGAAAAGAAAGTGGC
AAAACCCAAGGCTCCAGCCAAAAAGAAGACAGAAGCCTGA

Upstream 100 bases:

>100_bases
AACCGCTACCAGCGCCTGAACTAGGCACCTATATATATTGACGGCTTGCCAAGCCGCTCGCCGGGCACTTATCATGCCCC
GGCACCCAACAGGCCAAACC

Downstream 100 bases:

>100_bases
TTGGCCTGCTGCTTGAAGCAAAAAGCCCTCGACAAAATCGAGGGCTTTTTCATTTCAGGCGCCGGAATGCACTTTCCGTA
CGCGTTCCACGTGAAACCTC

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 839; Mature: 838

Protein sequence:

>839_residues
MSKKLIIAEKPSVAADIARALGGFTKHDDYFESEELVLSSAVGHLLELACPEEFEVKRGKWSFAHLPVIPPHFALKPIEK
TESRLKVLNKLIKRKDVTGLINACDAGREGELIFNYIAQNAKSGKPVQRLWLQSMTQGAIRDGFSRLRPGQEMEGLGDAA
VCRSESDWLVGINGTRAMTAFNSKTGGFHLTTVGRVQTPTLAIVVERERKIRDFKSRDYWEVEADFTAKAGSYKGKWFDE
AFKGKNDDEHARADRLWEVARAEAIRAATEGKPGEVTEEAKPETRLSPALFDLTTLQREANARFGFSAKTTLSLAQALYE
KHKVLTYPRTDSRCLPEDYLPTVKETLTILTGEGAGKGHDEVLLARYSPFAHQILARNWVLPNKRIFNNAKISDHFAIIP
TPQAPKSLNEMEQKLYDFVVRRFLSVFFPAAEYMVTTRITRVEGHPFKTEGKVLVNPGWLAVYGKEGQDGDEGNLVAVDA
KEKVKTDEVTVKANATKPPPRYSEATLLSAMEGAGKMVDDEELKAAMAGRGLGTPATRAQIIENLIGEQYMHREGRELIP
TAKAFTLMTLLNGLGIKELTQPELTGDWEWKLGRIEKGEFTRDEFMREIAEMTRHMVERAKQYEADTIPGDFGVLTAPCP
KCGGQIRETYKKFQCGSCDYALWKIVAGRQYEPAEIEQLLTEKQVGPLTGFRNKMGRSFSAAIKLNDELQPEFDFGQDKA
DAADAEPVDFSAQESLGKCPKCAANVFEHGTSYVCEKSVGPAKTCDFRSGKIILQQPIDAAQMKKLLADGKTDLLKEFVS
NRTRRKFSAYLVNQGGKVGFEFEKKVAKPKAPAKKKTEA

Sequences:

>Translated_839_residues
MSKKLIIAEKPSVAADIARALGGFTKHDDYFESEELVLSSAVGHLLELACPEEFEVKRGKWSFAHLPVIPPHFALKPIEK
TESRLKVLNKLIKRKDVTGLINACDAGREGELIFNYIAQNAKSGKPVQRLWLQSMTQGAIRDGFSRLRPGQEMEGLGDAA
VCRSESDWLVGINGTRAMTAFNSKTGGFHLTTVGRVQTPTLAIVVERERKIRDFKSRDYWEVEADFTAKAGSYKGKWFDE
AFKGKNDDEHARADRLWEVARAEAIRAATEGKPGEVTEEAKPETRLSPALFDLTTLQREANARFGFSAKTTLSLAQALYE
KHKVLTYPRTDSRCLPEDYLPTVKETLTILTGEGAGKGHDEVLLARYSPFAHQILARNWVLPNKRIFNNAKISDHFAIIP
TPQAPKSLNEMEQKLYDFVVRRFLSVFFPAAEYMVTTRITRVEGHPFKTEGKVLVNPGWLAVYGKEGQDGDEGNLVAVDA
KEKVKTDEVTVKANATKPPPRYSEATLLSAMEGAGKMVDDEELKAAMAGRGLGTPATRAQIIENLIGEQYMHREGRELIP
TAKAFTLMTLLNGLGIKELTQPELTGDWEWKLGRIEKGEFTRDEFMREIAEMTRHMVERAKQYEADTIPGDFGVLTAPCP
KCGGQIRETYKKFQCGSCDYALWKIVAGRQYEPAEIEQLLTEKQVGPLTGFRNKMGRSFSAAIKLNDELQPEFDFGQDKA
DAADAEPVDFSAQESLGKCPKCAANVFEHGTSYVCEKSVGPAKTCDFRSGKIILQQPIDAAQMKKLLADGKTDLLKEFVS
NRTRRKFSAYLVNQGGKVGFEFEKKVAKPKAPAKKKTEA
>Mature_838_residues
SKKLIIAEKPSVAADIARALGGFTKHDDYFESEELVLSSAVGHLLELACPEEFEVKRGKWSFAHLPVIPPHFALKPIEKT
ESRLKVLNKLIKRKDVTGLINACDAGREGELIFNYIAQNAKSGKPVQRLWLQSMTQGAIRDGFSRLRPGQEMEGLGDAAV
CRSESDWLVGINGTRAMTAFNSKTGGFHLTTVGRVQTPTLAIVVERERKIRDFKSRDYWEVEADFTAKAGSYKGKWFDEA
FKGKNDDEHARADRLWEVARAEAIRAATEGKPGEVTEEAKPETRLSPALFDLTTLQREANARFGFSAKTTLSLAQALYEK
HKVLTYPRTDSRCLPEDYLPTVKETLTILTGEGAGKGHDEVLLARYSPFAHQILARNWVLPNKRIFNNAKISDHFAIIPT
PQAPKSLNEMEQKLYDFVVRRFLSVFFPAAEYMVTTRITRVEGHPFKTEGKVLVNPGWLAVYGKEGQDGDEGNLVAVDAK
EKVKTDEVTVKANATKPPPRYSEATLLSAMEGAGKMVDDEELKAAMAGRGLGTPATRAQIIENLIGEQYMHREGRELIPT
AKAFTLMTLLNGLGIKELTQPELTGDWEWKLGRIEKGEFTRDEFMREIAEMTRHMVERAKQYEADTIPGDFGVLTAPCPK
CGGQIRETYKKFQCGSCDYALWKIVAGRQYEPAEIEQLLTEKQVGPLTGFRNKMGRSFSAAIKLNDELQPEFDFGQDKAD
AADAEPVDFSAQESLGKCPKCAANVFEHGTSYVCEKSVGPAKTCDFRSGKIILQQPIDAAQMKKLLADGKTDLLKEFVSN
RTRRKFSAYLVNQGGKVGFEFEKKVAKPKAPAKKKTEA

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=649, Percent_Identity=24.3451463790447, Blast_Score=122, Evalue=2e-27,
Organism=Escherichia coli, GI1788061, Length=650, Percent_Identity=31.6923076923077, Blast_Score=291, Evalue=9e-80,
Organism=Escherichia coli, GI1787529, Length=708, Percent_Identity=27.2598870056497, Blast_Score=142, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI32563869, Length=638, Percent_Identity=25.705329153605, Blast_Score=122, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17555378, Length=659, Percent_Identity=24.2792109256449, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24585251, Length=635, Percent_Identity=25.1968503937008, Blast_Score=118, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 93441; Mature: 93309

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKLIIAEKPSVAADIARALGGFTKHDDYFESEELVLSSAVGHLLELACPEEFEVKRGK
CCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCC
WSFAHLPVIPPHFALKPIEKTESRLKVLNKLIKRKDVTGLINACDAGREGELIFNYIAQN
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHC
AKSGKPVQRLWLQSMTQGAIRDGFSRLRPGQEMEGLGDAAVCRSESDWLVGINGTRAMTA
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHCCCCCCEEEEECCCEEEEE
FNSKTGGFHLTTVGRVQTPTLAIVVERERKIRDFKSRDYWEVEADFTAKAGSYKGKWFDE
ECCCCCCEEEEEECCCCCCEEEEEEECHHHHHHHCCCCCEEEECCCEECCCCCCCCHHHH
AFKGKNDDEHARADRLWEVARAEAIRAATEGKPGEVTEEAKPETRLSPALFDLTTLQREA
HHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
NARFGFSAKTTLSLAQALYEKHKVLTYPRTDSRCLPEDYLPTVKETLTILTGEGAGKGHD
CCCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHHEEEEECCCCCCCCC
EVLLARYSPFAHQILARNWVLPNKRIFNNAKISDHFAIIPTPQAPKSLNEMEQKLYDFVV
CEEEECCCHHHHHHHHHHCCCCCCHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
RRFLSVFFPAAEYMVTTRITRVEGHPFKTEGKVLVNPGWLAVYGKEGQDGDEGNLVAVDA
HHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEECCCEEEEECCCCCCCCCCCEEEECC
KEKVKTDEVTVKANATKPPPRYSEATLLSAMEGAGKMVDDEELKAAMAGRGLGTPATRAQ
HHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHH
IIENLIGEQYMHREGRELIPTAKAFTLMTLLNGLGIKELTQPELTGDWEWKLGRIEKGEF
HHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCC
TRDEFMREIAEMTRHMVERAKQYEADTIPGDFGVLTAPCPKCGGQIRETYKKFQCGSCDY
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCH
ALWKIVAGRQYEPAEIEQLLTEKQVGPLTGFRNKMGRSFSAAIKLNDELQPEFDFGQDKA
HHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCC
DAADAEPVDFSAQESLGKCPKCAANVFEHGTSYVCEKSVGPAKTCDFRSGKIILQQPIDA
CCCCCCCCCCCHHHHHCCCHHHHHHHHHCCCHHEECCCCCCCCCCCCCCCCEEEECCCCH
AQMKKLLADGKTDLLKEFVSNRTRRKFSAYLVNQGGKVGFEFEKKVAKPKAPAKKKTEA
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SKKLIIAEKPSVAADIARALGGFTKHDDYFESEELVLSSAVGHLLELACPEEFEVKRGK
CCCEEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCC
WSFAHLPVIPPHFALKPIEKTESRLKVLNKLIKRKDVTGLINACDAGREGELIFNYIAQN
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHC
AKSGKPVQRLWLQSMTQGAIRDGFSRLRPGQEMEGLGDAAVCRSESDWLVGINGTRAMTA
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHCCCCCCEEEEECCCEEEEE
FNSKTGGFHLTTVGRVQTPTLAIVVERERKIRDFKSRDYWEVEADFTAKAGSYKGKWFDE
ECCCCCCEEEEEECCCCCCEEEEEEECHHHHHHHCCCCCEEEECCCEECCCCCCCCHHHH
AFKGKNDDEHARADRLWEVARAEAIRAATEGKPGEVTEEAKPETRLSPALFDLTTLQREA
HHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
NARFGFSAKTTLSLAQALYEKHKVLTYPRTDSRCLPEDYLPTVKETLTILTGEGAGKGHD
CCCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHHEEEEECCCCCCCCC
EVLLARYSPFAHQILARNWVLPNKRIFNNAKISDHFAIIPTPQAPKSLNEMEQKLYDFVV
CEEEECCCHHHHHHHHHHCCCCCCHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
RRFLSVFFPAAEYMVTTRITRVEGHPFKTEGKVLVNPGWLAVYGKEGQDGDEGNLVAVDA
HHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEECCCEEEEECCCCCCCCCCCEEEECC
KEKVKTDEVTVKANATKPPPRYSEATLLSAMEGAGKMVDDEELKAAMAGRGLGTPATRAQ
HHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHH
IIENLIGEQYMHREGRELIPTAKAFTLMTLLNGLGIKELTQPELTGDWEWKLGRIEKGEF
HHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCC
TRDEFMREIAEMTRHMVERAKQYEADTIPGDFGVLTAPCPKCGGQIRETYKKFQCGSCDY
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCH
ALWKIVAGRQYEPAEIEQLLTEKQVGPLTGFRNKMGRSFSAAIKLNDELQPEFDFGQDKA
HHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCC
DAADAEPVDFSAQESLGKCPKCAANVFEHGTSYVCEKSVGPAKTCDFRSGKIILQQPIDA
CCCCCCCCCCCHHHHHCCCHHHHHHHHHCCCHHEECCCCCCCCCCCCCCCCEEEECCCCH
AQMKKLLADGKTDLLKEFVSNRTRRKFSAYLVNQGGKVGFEFEKKVAKPKAPAKKKTEA
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA