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The map label for this gene is 71905653

Identifier: 71905653

GI number: 71905653

Start: 15001

End: 16287

Strand: Reverse

Name: 71905653

Synonym: Daro_0011

Alternate gene names: NA

Gene position: 16287-15001 (Counterclockwise)

Preceding gene: 71905654

Following gene: 71905652

Centisome position: 0.36

GC content: 62.47

Gene sequence:

>1287_bases
ATGGTTCCACACCTCACCACAGCCCTCACCGGCCCCCTGCTCGAACTGGAGCGTCGCTTCATCGCCGCCAGTCCGCAAAT
CGAACACTGGCTGCGCGGCCAGTGGCTGGAACACACGCCGCCGTTCTACTCGTCGTGCGACCTGCGCAATTCCGGCTTCA
AGCTGGCACCGGTCGATACCAACCTGTTCCCGGGCGGTTTCAACAACCTGAACCCCGCCTTCCTGCCGCTTTGCGTGCAG
GCGGCGATGAGCGCCATCGAAAAATTCTGTCCGGAAGCCCGCAGCCTGCTGCTGATCCCGGAAAACCACACGCGCAACCA
GTTCTACCTGCAGAACGTCGCGCAGATCGCCGCCATTCTCAAGCAGACCGGCCTGAATGTGCGCCTCGGTTCGCTGAACC
CGGAAGTCACCCAGCCGACGACTATCGAACTGCCCAACGGCCAGAGCCTGCTGCTCGAACCACTGGTTCGCACCAAGAAC
CGCCTTGGGCTGGACGGTTTCGACCCCTGCGCCATCCTGCTCAACAACGACCTGTCGGCCGGCATTCCGGCCATCCTGCA
AGGCCTCCACGAGCAGGTCGTTCTGCCGCCGGTGCATGCCGGCTGGGCGGTACGTCGCAAGTCCAACCACTTCGCTGCCT
ACGATCAGGTCGCCAACGACTTTGCCAAGGCCATCGGCATCGATCCGTGGCGTATCAACCCGGCCTTCTCGGTCTGCCGC
AGCGTCAATTTCCACGAACGCCAAGGTGAGGAATGCCTGGCCGCCAATGTTTCGGCTGTGCTCGACATCGTCAAGGAAAA
GTACCGCGAATACGACATCGAAGAAACGCCCTACGTCGTCGTCAAAGCCGATGCCGGCACCTACGGCATGGGCGTGATGA
CGGTGCGCAACGTCGATGAGGTCATCGCGCTGAACCGCAAGCAGCGCAACAAGATGAGCGTCGTCAAGGAAGGTCTGGAA
GTCAGCGAGGTACTGATCCAGGAAGGCGTGCATTCGTTTGAGACCCTGAACGATGCGGTGGCCGAACCGGTCATCTACAT
GATCGACCGCTATGTCGTCGGCGGCTTCTACCGTGTCCATACCGGGCGCGGCAAGGACGAGAACCTCAACGCGCCGGGCA
TGCACTTCGAGCCGCTGGCCTTCGAGACTGGCTGCAACATGCCCGACTACGGCTGTGGCAACCCGGACGCACCACCCAAC
CGCTTCTACGCGTATGGCGTCGTCGGCCGCCTGGCCTGCCTGGCCGCTGCCATCGAGCTGGAACGCACTGCGCCGGAAGA
CGTCTGA

Upstream 100 bases:

>100_bases
GAACGCCTCGCACAAGCCGACTACCGGCTATAGTCCCTTTTGTTGCACCGCAGTGTAAGATTTGCGGCGATAACAAAAAC
CTCGGATTGAGACACAGCAT

Downstream 100 bases:

>100_bases
GTCGGCATGGCTCAGCAACTGCACTTTGAAAAACACCTGCTCGGCGGCAGCAGCCAGTCCCTGAAACTGGCTTTTGTGCT
CGATCCGCTGGACCATCTGA

Product: glutamate--cysteine ligase GshA

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Glutamate-Cysteine Ligase GshA; Glutamate-Cysteine Ligase-Related Protein; Glutathione Synthetase; GshA Protein

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MVPHLTTALTGPLLELERRFIAASPQIEHWLRGQWLEHTPPFYSSCDLRNSGFKLAPVDTNLFPGGFNNLNPAFLPLCVQ
AAMSAIEKFCPEARSLLLIPENHTRNQFYLQNVAQIAAILKQTGLNVRLGSLNPEVTQPTTIELPNGQSLLLEPLVRTKN
RLGLDGFDPCAILLNNDLSAGIPAILQGLHEQVVLPPVHAGWAVRRKSNHFAAYDQVANDFAKAIGIDPWRINPAFSVCR
SVNFHERQGEECLAANVSAVLDIVKEKYREYDIEETPYVVVKADAGTYGMGVMTVRNVDEVIALNRKQRNKMSVVKEGLE
VSEVLIQEGVHSFETLNDAVAEPVIYMIDRYVVGGFYRVHTGRGKDENLNAPGMHFEPLAFETGCNMPDYGCGNPDAPPN
RFYAYGVVGRLACLAAAIELERTAPEDV

Sequences:

>Translated_428_residues
MVPHLTTALTGPLLELERRFIAASPQIEHWLRGQWLEHTPPFYSSCDLRNSGFKLAPVDTNLFPGGFNNLNPAFLPLCVQ
AAMSAIEKFCPEARSLLLIPENHTRNQFYLQNVAQIAAILKQTGLNVRLGSLNPEVTQPTTIELPNGQSLLLEPLVRTKN
RLGLDGFDPCAILLNNDLSAGIPAILQGLHEQVVLPPVHAGWAVRRKSNHFAAYDQVANDFAKAIGIDPWRINPAFSVCR
SVNFHERQGEECLAANVSAVLDIVKEKYREYDIEETPYVVVKADAGTYGMGVMTVRNVDEVIALNRKQRNKMSVVKEGLE
VSEVLIQEGVHSFETLNDAVAEPVIYMIDRYVVGGFYRVHTGRGKDENLNAPGMHFEPLAFETGCNMPDYGCGNPDAPPN
RFYAYGVVGRLACLAAAIELERTAPEDV
>Mature_428_residues
MVPHLTTALTGPLLELERRFIAASPQIEHWLRGQWLEHTPPFYSSCDLRNSGFKLAPVDTNLFPGGFNNLNPAFLPLCVQ
AAMSAIEKFCPEARSLLLIPENHTRNQFYLQNVAQIAAILKQTGLNVRLGSLNPEVTQPTTIELPNGQSLLLEPLVRTKN
RLGLDGFDPCAILLNNDLSAGIPAILQGLHEQVVLPPVHAGWAVRRKSNHFAAYDQVANDFAKAIGIDPWRINPAFSVCR
SVNFHERQGEECLAANVSAVLDIVKEKYREYDIEETPYVVVKADAGTYGMGVMTVRNVDEVIALNRKQRNKMSVVKEGLE
VSEVLIQEGVHSFETLNDAVAEPVIYMIDRYVVGGFYRVHTGRGKDENLNAPGMHFEPLAFETGCNMPDYGCGNPDAPPN
RFYAYGVVGRLACLAAAIELERTAPEDV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 47331; Mature: 47331

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPHLTTALTGPLLELERRFIAASPQIEHWLRGQWLEHTPPFYSSCDLRNSGFKLAPVDT
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCC
NLFPGGFNNLNPAFLPLCVQAAMSAIEKFCPEARSLLLIPENHTRNQFYLQNVAQIAAIL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHCCEEEECCCCCCCHHHHHHHHHHHHHH
KQTGLNVRLGSLNPEVTQPTTIELPNGQSLLLEPLVRTKNRLGLDGFDPCAILLNNDLSA
HHCCCEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCC
GIPAILQGLHEQVVLPPVHAGWAVRRKSNHFAAYDQVANDFAKAIGIDPWRINPAFSVCR
CHHHHHHHHHHCEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
SVNFHERQGEECLAANVSAVLDIVKEKYREYDIEETPYVVVKADAGTYGMGVMTVRNVDE
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEHHCHHH
VIALNRKQRNKMSVVKEGLEVSEVLIQEGVHSFETLNDAVAEPVIYMIDRYVVGGFYRVH
HHHHCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
TGRGKDENLNAPGMHFEPLAFETGCNMPDYGCGNPDAPPNRFYAYGVVGRLACLAAAIEL
CCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHH
ERTAPEDV
HCCCCCCC
>Mature Secondary Structure
MVPHLTTALTGPLLELERRFIAASPQIEHWLRGQWLEHTPPFYSSCDLRNSGFKLAPVDT
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCC
NLFPGGFNNLNPAFLPLCVQAAMSAIEKFCPEARSLLLIPENHTRNQFYLQNVAQIAAIL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHCCEEEECCCCCCCHHHHHHHHHHHHHH
KQTGLNVRLGSLNPEVTQPTTIELPNGQSLLLEPLVRTKNRLGLDGFDPCAILLNNDLSA
HHCCCEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCC
GIPAILQGLHEQVVLPPVHAGWAVRRKSNHFAAYDQVANDFAKAIGIDPWRINPAFSVCR
CHHHHHHHHHHCEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
SVNFHERQGEECLAANVSAVLDIVKEKYREYDIEETPYVVVKADAGTYGMGVMTVRNVDE
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEHHCHHH
VIALNRKQRNKMSVVKEGLEVSEVLIQEGVHSFETLNDAVAEPVIYMIDRYVVGGFYRVH
HHHHCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
TGRGKDENLNAPGMHFEPLAFETGCNMPDYGCGNPDAPPNRFYAYGVVGRLACLAAAIEL
CCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHH
ERTAPEDV
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA