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The map label for this gene is nagE [H]

Identifier: 71893924

GI number: 71893924

Start: 742727

End: 744436

Strand: Reverse

Name: nagE [H]

Synonym: MHJ_0575

Alternate gene names: 71893924

Gene position: 744436-742727 (Counterclockwise)

Preceding gene: 71893925

Following gene: 71893923

Centisome position: 82.95

GC content: 33.86

Gene sequence:

>1710_bases
ATTTTTAACCGAATTTTGAATCAATTACAGCGGCTTGGTAGATCACTAATGTTCCCAATTGCCGTTTTACCGATTGCGGC
ACTTTTATTACGAATTGGTGCACTAATTCAGGATCCTTTAGCAAATGGATCAATTGGAATATCCGAGACGCAAAAATTTT
GGGGAAGGCTGATTTCAACCCCAGGCGGAATAGTTTTTGATAATCTAGCGATAATTTTTGCAATTGGAATCGGTTTTGGT
TTTGCCAAAGACAACCGTGGCGAGGCCGCTCTTGTAGCTGCTGTTGTTTGATATGGGATGTCAACACTTCTAAAACAGAA
CCAATTAGCGGAGGCAATTTATAAAAATGTTTTAGTTGCTAAGTCCGGTCAACTCAAAGGATTTACGCAACTTTTGTATT
TTTTAAAAGCAGGAAAGCCAGTTTATCAGCTCGATTCAGGAGTTATTGGCGGAATTAGTGTTGGACTTGTTGTTGCATTT
TTGTATAATAAATACAAAAATATTAAACTACCACAAACACTTTCTTTTTTTGGAGGCCGCAGATTTGTGCCTATGTTAGG
AATTCTTTCAATAATTCCGCTTGGTTTGATATTTGCAATTATTTGACCTTGAGCCCAATATGGACTAATTAAAATTGGTT
CAGCTCTATCAGATCAAAGTGCAAATCATTATGCTCGAGGGTTTTATGTTTCAATTTATGCATTTTTAAATAGATTATTA
CAACCTTTTGGACTTCATCATATTTTGAATACTTTTGTCTGATTTCAATTGCCAATTAAGGGTAAATTAATTTCCCCCGT
GATTAATCAGGTGGGAGAAACTGTTGCAAAATTAGGTGAAATTTATACTGTAAATGGGGATATAACTGCATTTAATACTG
GAATTATTGGCTCTGGTGGGTTTACAACCGGATTTTTCCCGTTATATTTAGGGGGGCTTCCTGGAGCTGCAGTTGCAATG
ATTTTTGCTGCAAAAAAAGAAAGAAGAAAAACAATTACTACCTTTTTAGCAGGAGCGACACTTGTAAGTTTTCTTACTGG
AATTGATGAGCCTTTAATTTTTACTTTTATTTTCATCTCACCACTTTTATGACTTTTAAATGCTTTTTTGACCTCAGTGA
TTTACATGTTTGTATCTTGAACTGGAATGAGTATCGGAATTGGATTTTCTGCTGGATTTATTGATTATATTGTTTCTTTT
CCAAGGTCATGGGCGTTTGCAAAAAACTTTGGAGTTTTGGCAAATCCACTATGAATTTGGGTTTTTTCCGCGATAATGTT
TGTAATTCAAGGTTCAAGTTTCTTCTTTTGTATTAAAAAGTTTAATATTAAAACTTTGGGTCGTGAAGATAAAATTGGGC
CAGAATTTAGTATAATAGATGAAAAAACTGACTTGCAAAATCAGGAAATATTTCTACCTGATCAAGAAAAAATAACTAAA
AATAAAGTAAAATTTGCTGATGACTATCAAAAAATGGCAGCAGATTTTATTGAAATTTTAGGTAAGGAAAATATTGAAGA
AGTCTCAAATTGTGCAACACGGCTAAGATTAATTCTTAAAGATAATGCAAAAAATGATGAATTAGATGCAAAAATTACAG
CCGCCGGCGGCCGTGGAGTTGTCCGTGTTGGAAATAAAGGATATCAAATTATTATTGGAACCGATGTTGAATTTGTAGCT
GATTATCTTAAAAAAATGATAGGAAAATAA

Upstream 100 bases:

>100_bases
ATTAAAAAATTTAGAAAAACTAGTTATAATGTCAGTAGGATTTGTAAAAAAAATAAAGTTTTTTCGGCCAAAAAAGGCCG
AAAATTATAGTCAAAATTCG

Downstream 100 bases:

>100_bases
TGGTTTTTATCGATCCAAAAGAAACATTTTCAAAACTAAATGCAGAGAATAAACCAATTTTTGCTTTTAATGTTATAAAT
TTAGAAACACTTTTTGCTGT

Product: putative PTS system N-acetylglucosamine-specific transporter subunit IIABC

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 569; Mature: 569

Protein sequence:

>569_residues
MFNRILNQLQRLGRSLMFPIAVLPIAALLLRIGALIQDPLANGSIGISETQKFWGRLISTPGGIVFDNLAIIFAIGIGFG
FAKDNRGEAALVAAVVWYGMSTLLKQNQLAEAIYKNVLVAKSGQLKGFTQLLYFLKAGKPVYQLDSGVIGGISVGLVVAF
LYNKYKNIKLPQTLSFFGGRRFVPMLGILSIIPLGLIFAIIWPWAQYGLIKIGSALSDQSANHYARGFYVSIYAFLNRLL
QPFGLHHILNTFVWFQLPIKGKLISPVINQVGETVAKLGEIYTVNGDITAFNTGIIGSGGFTTGFFPLYLGGLPGAAVAM
IFAAKKERRKTITTFLAGATLVSFLTGIDEPLIFTFIFISPLLWLLNAFLTSVIYMFVSWTGMSIGIGFSAGFIDYIVSF
PRSWAFAKNFGVLANPLWIWVFSAIMFVIQGSSFFFCIKKFNIKTLGREDKIGPEFSIIDEKTDLQNQEIFLPDQEKITK
NKVKFADDYQKMAADFIEILGKENIEEVSNCATRLRLILKDNAKNDELDAKITAAGGRGVVRVGNKGYQIIIGTDVEFVA
DYLKKMIGK

Sequences:

>Translated_569_residues
MFNRILNQLQRLGRSLMFPIAVLPIAALLLRIGALIQDPLANGSIGISETQKFWGRLISTPGGIVFDNLAIIFAIGIGFG
FAKDNRGEAALVAAVV*YGMSTLLKQNQLAEAIYKNVLVAKSGQLKGFTQLLYFLKAGKPVYQLDSGVIGGISVGLVVAF
LYNKYKNIKLPQTLSFFGGRRFVPMLGILSIIPLGLIFAII*P*AQYGLIKIGSALSDQSANHYARGFYVSIYAFLNRLL
QPFGLHHILNTFV*FQLPIKGKLISPVINQVGETVAKLGEIYTVNGDITAFNTGIIGSGGFTTGFFPLYLGGLPGAAVAM
IFAAKKERRKTITTFLAGATLVSFLTGIDEPLIFTFIFISPLL*LLNAFLTSVIYMFVS*TGMSIGIGFSAGFIDYIVSF
PRSWAFAKNFGVLANPL*IWVFSAIMFVIQGSSFFFCIKKFNIKTLGREDKIGPEFSIIDEKTDLQNQEIFLPDQEKITK
NKVKFADDYQKMAADFIEILGKENIEEVSNCATRLRLILKDNAKNDELDAKITAAGGRGVVRVGNKGYQIIIGTDVEFVA
DYLKKMIGK
>Mature_569_residues
MFNRILNQLQRLGRSLMFPIAVLPIAALLLRIGALIQDPLANGSIGISETQKFWGRLISTPGGIVFDNLAIIFAIGIGFG
FAKDNRGEAALVAAVV*YGMSTLLKQNQLAEAIYKNVLVAKSGQLKGFTQLLYFLKAGKPVYQLDSGVIGGISVGLVVAF
LYNKYKNIKLPQTLSFFGGRRFVPMLGILSIIPLGLIFAII*P*AQYGLIKIGSALSDQSANHYARGFYVSIYAFLNRLL
QPFGLHHILNTFV*FQLPIKGKLISPVINQVGETVAKLGEIYTVNGDITAFNTGIIGSGGFTTGFFPLYLGGLPGAAVAM
IFAAKKERRKTITTFLAGATLVSFLTGIDEPLIFTFIFISPLL*LLNAFLTSVIYMFVS*TGMSIGIGFSAGFIDYIVSF
PRSWAFAKNFGVLANPL*IWVFSAIMFVIQGSSFFFCIKKFNIKTLGREDKIGPEFSIIDEKTDLQNQEIFLPDQEKITK
NKVKFADDYQKMAADFIEILGKENIEEVSNCATRLRLILKDNAKNDELDAKITAAGGRGVVRVGNKGYQIIIGTDVEFVA
DYLKKMIGK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=565, Percent_Identity=35.5752212389381, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1787343, Length=561, Percent_Identity=31.9073083778966, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI1787908, Length=571, Percent_Identity=28.0210157618214, Blast_Score=172, Evalue=6e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 61340; Mature: 61340

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEEEEECCHHHHHHHHHHHHCC
>Mature Secondary Structure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EEEEEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3284790; 3056518; 8905232; 9278503 [H]