BacMap

Definition	Corynebacterium jeikeium K411, complete genome.
Accession	NC_007164
Length	2,462,499

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The map label for this gene is fadD4 [H]

Identifier: 68536433

GI number: 68536433

Start: 1593679

End: 1595367

Strand: Direct

Name: fadD4 [H]

Synonym: jk1348

Alternate gene names: 68536433

Gene position: 1593679-1595367 (Clockwise)

Preceding gene: 68536415

Following gene: 68536434

Centisome position: 64.72

GC content: 61.34

Gene sequence:

>1689_bases
ATGAAGTCGACGATGCAGGAGATCCCACTTTCCGTTGCGAGGATCCTGGAATACGGGGCCAGCGTTCACCGAAACACCAC
CGTGACCACGTATTTTGACGAAGCTGCGGAACAAACTAGCTTCCTGCACATCGGCATCCGCGCAGCCGCGATGGCCAACA
TGCTGCGCGACGAGTTTGGCATCGAGCGGGGCGACCGCGTGGGCACCGTCCTGCCGAACTGCACCGAACACCTTGAGGTG
CTGCTCTCCGTAGCCTCGATGGGTGCGGTGTTTAACCCCATCAACCGGCACCTCATGGACACCCAGATCACGCACATCAT
CAACAAGGCCGCGCCGAAAGTGCTGGTTCTCGACCCGGCTTGTAAAGAACAGATCATCCCGCTGCTAGCCGACTGCCCCT
GCGTAGAAGCGGTGCTGGTGATCGGCCCGGACTTCGCTGACACCGAAGCTGTACAAAAGCTCGCCACAGCACGGCGCGAT
CAGGGCGAAAAGGGCACGCCCGGCTTGAGCCACCTGCGCATCCTAAACCTGGAGGCCGAGTTGGATGGGCGTAGCGCAGA
CTTCGACTGGCCCGAAGTGGAAGAAACTGACCCGGCGGCTATCTGCTTTTCCACTGGTACAGAGGGCCCGCCGAAGGGCG
TGGTCTATTCCCATCGCGCGCTGTGGCTGCACTCCATGCAGCTGCGCGCCGCCGATAGCTTCAGCATCCGCAACGGCACT
AGCTTCCTGTGCTGCGTGCCGATCTATCACGTACTCAGTTGGGGCGTGCCACTGGCAGCTTTTATGGCCGGCGCACCGAT
CGTGTTTACCGGACGCAGCGCCACTCCAGAACACCTGGCGCACGTAATCGAAGACGCCATGCCGCGGCAAGCGCACGGAT
CGCCGGCAGTATGGACCGGCCTGCTGGTGCATTACGCCAAGAAGCACCCGAAGAAGATGAGCCTGCAGGAGATCTACGTT
GGCGGTTCACAGGTCTCCCCTGCCATGATCGATGCCTGGGAGGAGCGCTTCGGCGTGGACATCATCCACTCCTGGGGCAT
GACGGAGACCGGCCCGGTGGGCACCGTTGCCCACCCACCCGCCGGAGTGGCCGGCGCTGCTCGTGCTAAGTACCGCGAAA
GCCAAGGGCGCTTTCACGCGGGCATGCGCTACCGCATCGTCGACGACCACGACAATGTGCTGGAAGCCAACGACCGCAAC
GAAGGCGAGCTGCAGGTACGCGGCAACACGGTGACAGCCTCGTACTATAAGGACGACAGCCCGCGCTTTACCGAGGATGG
CTGGCTGCGCACCGGCGACATCGCCACCGTGAATAAGGACGGCTACCTGACAATTCACGACCGCAAGGCCGACATCATTC
GCTCTGGCGGTGAATGGATCTACTCTGCAGCGCTGGAAAACTACTTGTTGGAACCGGAAGCCGTGATCGAAGCCGCGGTG
ATCGGCATCCCATCAGAAAAATGGGGGCAGCGCCCGCTGGCAGTGGTAGTTGTGGCCGAAGGAACCCCGTGGACGGCAGA
AACCGCACAAGAACTGGCTAAAGAGTTACGCGAGCGCGTGCCCGGTTGGATGGTGCCGGAGAACTGGACCTTCGTGGATC
ACATCGACAAGACCAGCGTGGATAAGTTCGACAAGAAGGACCTGCGCCAGCACTTCCGCGAGGGGAAATTCGACATCATC
ACGGTCTAG

Upstream 100 bases:

>100_bases
GTAAAAAAGATAAGAGAAAGAAACGAGCCTCGCATCCGGCTCCCGTAGCTAACCATAGCACCTCCCACACCGCGAGTACC
TAACGGCTAGGCTGAATTGC

Downstream 100 bases:

>100_bases
GGTGCGCCTTGAAGGGCTGCAAGAGTCACTCCGGGCTGGTTGAGAGTAAATTCGCTAGCTTTTAACAGGCGCCACTCTAA
CTATTACCGGGCAAAGTAGA

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MKSTMQEIPLSVARILEYGASVHRNTTVTTYFDEAAEQTSFLHIGIRAAAMANMLRDEFGIERGDRVGTVLPNCTEHLEV
LLSVASMGAVFNPINRHLMDTQITHIINKAAPKVLVLDPACKEQIIPLLADCPCVEAVLVIGPDFADTEAVQKLATARRD
QGEKGTPGLSHLRILNLEAELDGRSADFDWPEVEETDPAAICFSTGTEGPPKGVVYSHRALWLHSMQLRAADSFSIRNGT
SFLCCVPIYHVLSWGVPLAAFMAGAPIVFTGRSATPEHLAHVIEDAMPRQAHGSPAVWTGLLVHYAKKHPKKMSLQEIYV
GGSQVSPAMIDAWEERFGVDIIHSWGMTETGPVGTVAHPPAGVAGAARAKYRESQGRFHAGMRYRIVDDHDNVLEANDRN
EGELQVRGNTVTASYYKDDSPRFTEDGWLRTGDIATVNKDGYLTIHDRKADIIRSGGEWIYSAALENYLLEPEAVIEAAV
IGIPSEKWGQRPLAVVVVAEGTPWTAETAQELAKELRERVPGWMVPENWTFVDHIDKTSVDKFDKKDLRQHFREGKFDII
TV

Sequences:

>Translated_562_residues
MKSTMQEIPLSVARILEYGASVHRNTTVTTYFDEAAEQTSFLHIGIRAAAMANMLRDEFGIERGDRVGTVLPNCTEHLEV
LLSVASMGAVFNPINRHLMDTQITHIINKAAPKVLVLDPACKEQIIPLLADCPCVEAVLVIGPDFADTEAVQKLATARRD
QGEKGTPGLSHLRILNLEAELDGRSADFDWPEVEETDPAAICFSTGTEGPPKGVVYSHRALWLHSMQLRAADSFSIRNGT
SFLCCVPIYHVLSWGVPLAAFMAGAPIVFTGRSATPEHLAHVIEDAMPRQAHGSPAVWTGLLVHYAKKHPKKMSLQEIYV
GGSQVSPAMIDAWEERFGVDIIHSWGMTETGPVGTVAHPPAGVAGAARAKYRESQGRFHAGMRYRIVDDHDNVLEANDRN
EGELQVRGNTVTASYYKDDSPRFTEDGWLRTGDIATVNKDGYLTIHDRKADIIRSGGEWIYSAALENYLLEPEAVIEAAV
IGIPSEKWGQRPLAVVVVAEGTPWTAETAQELAKELRERVPGWMVPENWTFVDHIDKTSVDKFDKKDLRQHFREGKFDII
TV
>Mature_562_residues
MKSTMQEIPLSVARILEYGASVHRNTTVTTYFDEAAEQTSFLHIGIRAAAMANMLRDEFGIERGDRVGTVLPNCTEHLEV
LLSVASMGAVFNPINRHLMDTQITHIINKAAPKVLVLDPACKEQIIPLLADCPCVEAVLVIGPDFADTEAVQKLATARRD
QGEKGTPGLSHLRILNLEAELDGRSADFDWPEVEETDPAAICFSTGTEGPPKGVVYSHRALWLHSMQLRAADSFSIRNGT
SFLCCVPIYHVLSWGVPLAAFMAGAPIVFTGRSATPEHLAHVIEDAMPRQAHGSPAVWTGLLVHYAKKHPKKMSLQEIYV
GGSQVSPAMIDAWEERFGVDIIHSWGMTETGPVGTVAHPPAGVAGAARAKYRESQGRFHAGMRYRIVDDHDNVLEANDRN
EGELQVRGNTVTASYYKDDSPRFTEDGWLRTGDIATVNKDGYLTIHDRKADIIRSGGEWIYSAALENYLLEPEAVIEAAV
IGIPSEKWGQRPLAVVVVAEGTPWTAETAQELAKELRERVPGWMVPENWTFVDHIDKTSVDKFDKKDLRQHFREGKFDII
TV

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI58082049, Length=531, Percent_Identity=25.2354048964218, Blast_Score=126, Evalue=7e-29,
Organism=Homo sapiens, GI157311624, Length=531, Percent_Identity=24.2937853107345, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI157311622, Length=531, Percent_Identity=24.2937853107345, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI156151445, Length=513, Percent_Identity=25.9259259259259, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI122937307, Length=537, Percent_Identity=22.5325884543762, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI187761345, Length=386, Percent_Identity=27.720207253886, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI187761343, Length=386, Percent_Identity=27.720207253886, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI38505220, Length=534, Percent_Identity=24.3445692883895, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI115511026, Length=537, Percent_Identity=22.5325884543762, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI13375727, Length=569, Percent_Identity=24.0773286467487, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI42544132, Length=532, Percent_Identity=22.1804511278195, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI28416953, Length=518, Percent_Identity=23.7451737451737, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI253970489, Length=561, Percent_Identity=21.74688057041, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI8923896, Length=555, Percent_Identity=21.981981981982, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI1788107, Length=541, Percent_Identity=24.2144177449168, Blast_Score=137, Evalue=3e-33,
Organism=Escherichia coli, GI145693145, Length=511, Percent_Identity=25.2446183953033, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI221142682, Length=534, Percent_Identity=22.4719101123595, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786810, Length=389, Percent_Identity=26.2210796915167, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1789201, Length=359, Percent_Identity=25.0696378830084, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1790505, Length=538, Percent_Identity=23.9776951672862, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17558820, Length=514, Percent_Identity=25.4863813229572, Blast_Score=163, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI32563687, Length=531, Percent_Identity=25.0470809792844, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI71994703, Length=498, Percent_Identity=23.2931726907631, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI71994694, Length=467, Percent_Identity=24.1970021413276, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI71994690, Length=498, Percent_Identity=23.2931726907631, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17560140, Length=320, Percent_Identity=25.9375, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17559526, Length=369, Percent_Identity=27.10027100271, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17560308, Length=506, Percent_Identity=24.1106719367589, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17531443, Length=378, Percent_Identity=24.8677248677249, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17557194, Length=575, Percent_Identity=21.9130434782609, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71985884, Length=368, Percent_Identity=23.6413043478261, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71996755, Length=500, Percent_Identity=22.2, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17538037, Length=513, Percent_Identity=20.6627680311891, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71983001, Length=512, Percent_Identity=22.4609375, Blast_Score=65, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI71982997, Length=511, Percent_Identity=22.3091976516634, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=365, Percent_Identity=25.2054794520548, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319264, Length=524, Percent_Identity=21.7557251908397, Blast_Score=90, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24581924, Length=542, Percent_Identity=25.4612546125461, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI18859661, Length=536, Percent_Identity=24.8134328358209, Blast_Score=126, Evalue=5e-29,
Organism=Drosophila melanogaster, GI21355181, Length=547, Percent_Identity=23.7659963436929, Blast_Score=122, Evalue=8e-28,
Organism=Drosophila melanogaster, GI19922652, Length=557, Percent_Identity=22.262118491921, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24656500, Length=571, Percent_Identity=23.292469352014, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24653035, Length=484, Percent_Identity=22.5206611570248, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24648260, Length=483, Percent_Identity=23.8095238095238, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI281365686, Length=249, Percent_Identity=27.710843373494, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356947, Length=499, Percent_Identity=21.8436873747495, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI62472339, Length=549, Percent_Identity=22.7686703096539, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI161076582, Length=491, Percent_Identity=22.4032586558045, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24667955, Length=549, Percent_Identity=22.7686703096539, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21358303, Length=566, Percent_Identity=23.1448763250883, Blast_Score=74, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 62030; Mature: 62030

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSTMQEIPLSVARILEYGASVHRNTTVTTYFDEAAEQTSFLHIGIRAAAMANMLRDEFG
CCCCHHHHHHHHHHHHHHCCHHCCCCEEEEEEHHHHCCCEEEEEHHHHHHHHHHHHHHHC
IERGDRVGTVLPNCTEHLEVLLSVASMGAVFNPINRHLMDTQITHIINKAAPKVLVLDPA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCH
CKEQIIPLLADCPCVEAVLVIGPDFADTEAVQKLATARRDQGEKGTPGLSHLRILNLEAE
HHHHHHHHHCCCCHHHEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEC
LDGRSADFDWPEVEETDPAAICFSTGTEGPPKGVVYSHRALWLHSMQLRAADSFSIRNGT
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEECCHHHHHHHHHHCCCCCCEECCCC
SFLCCVPIYHVLSWGVPLAAFMAGAPIVFTGRSATPEHLAHVIEDAMPRQAHGSPAVWTG
CEEEHHHHHHHHHCCCHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHH
LLVHYAKKHPKKMSLQEIYVGGSQVSPAMIDAWEERFGVDIIHSWGMTETGPVGTVAHPP
HHHHHHHCCCCCCCHHHEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCC
AGVAGAARAKYRESQGRFHAGMRYRIVDDHDNVLEANDRNEGELQVRGNTVTASYYKDDS
CCCCHHHHHHHHHHCCCEECCEEEEEECCCCCEECCCCCCCCEEEEECCEEEEEEEECCC
PRFTEDGWLRTGDIATVNKDGYLTIHDRKADIIRSGGEWIYSAALENYLLEPEAVIEAAV
CCCCCCCCEEECCEEEECCCCEEEEECCCHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHH
IGIPSEKWGQRPLAVVVVAEGTPWTAETAQELAKELRERVPGWMVPENWTFVDHIDKTSV
CCCCCHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEHCCCCCCCH
DKFDKKDLRQHFREGKFDIITV
HHCCHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MKSTMQEIPLSVARILEYGASVHRNTTVTTYFDEAAEQTSFLHIGIRAAAMANMLRDEFG
CCCCHHHHHHHHHHHHHHCCHHCCCCEEEEEEHHHHCCCEEEEEHHHHHHHHHHHHHHHC
IERGDRVGTVLPNCTEHLEVLLSVASMGAVFNPINRHLMDTQITHIINKAAPKVLVLDPA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCH
CKEQIIPLLADCPCVEAVLVIGPDFADTEAVQKLATARRDQGEKGTPGLSHLRILNLEAE
HHHHHHHHHCCCCHHHEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEC
LDGRSADFDWPEVEETDPAAICFSTGTEGPPKGVVYSHRALWLHSMQLRAADSFSIRNGT
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEECCHHHHHHHHHHCCCCCCEECCCC
SFLCCVPIYHVLSWGVPLAAFMAGAPIVFTGRSATPEHLAHVIEDAMPRQAHGSPAVWTG
CEEEHHHHHHHHHCCCHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHH
LLVHYAKKHPKKMSLQEIYVGGSQVSPAMIDAWEERFGVDIIHSWGMTETGPVGTVAHPP
HHHHHHHCCCCCCCHHHEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCC
AGVAGAARAKYRESQGRFHAGMRYRIVDDHDNVLEANDRNEGELQVRGNTVTASYYKDDS
CCCCHHHHHHHHHHCCCEECCEEEEEECCCCCEECCCCCCCCEEEEECCEEEEEEEECCC
PRFTEDGWLRTGDIATVNKDGYLTIHDRKADIIRSGGEWIYSAALENYLLEPEAVIEAAV
CCCCCCCCEEECCEEEECCCCEEEEECCCHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHH
IGIPSEKWGQRPLAVVVVAEGTPWTAETAQELAKELRERVPGWMVPENWTFVDHIDKTSV
CCCCCHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEHCCCCCCCH
DKFDKKDLRQHFREGKFDIITV
HHCCHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]