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The map label for this gene is mdh

Identifier: 68535620

GI number: 68535620

Start: 664104

End: 665096

Strand: Direct

Name: mdh

Synonym: jk0548

Alternate gene names: 68535620

Gene position: 664104-665096 (Clockwise)

Preceding gene: 68535618

Following gene: 68535621

Centisome position: 26.97

GC content: 65.36

Gene sequence:

>993_bases
GTGACCACAAACAACCAGAACGCCGTTACCGTCACCGTCACGGGTGCAGGCGGCCAGATCGGCTACTCCCTGCTGTTCCG
CATCGCCAAGGGAGAGGTCTTCGGCGACCGCGTAGTGAACCTGCGTCTGCTGGAGACCGAGCAGGGCGTGCAGGCAGCAC
GCGGTGTGGCCCTGGAACTGGCCGACTGCGCCTTCCCTCTGCTGGGGGAGGTTAGCATCACCACCGAGCTCAGCGAGGGG
TTCAAGGATGCCAACGCAGTCTTCCTGGTGGGCGCTAAGCCTCGCCAGAAGGGCGAGGAGCGTGCCGACCTGCTGGCCGC
CAACGGCAAGATTTTTGGCCCGCAGGGCAAAGCGATCGCAGAGTACGCCGCCGATGATGTCCGCGTGATCGTCGTCGGTA
ACCCGGCCAACACCAACGCCGCAATCGTTGCCGCTCACGCCGAAGGCCTCGACCCGCGCCGAGTGACCGCACTTACTCGT
CTGGACCACAACCGTGGCCTGGCACAGGTGGCAGACAAACTGGGCGTGGCGGTGCGCGACCTGAAGAACATGACTGTCTG
GGGCAACCACTCTGCCTCCCAGTTCCCGGACGTTGCGGAGCTGACGCTGAATGGTGAAAAGGTCGCCGACAAGCTGGACG
CGGCGTGGGTAAACGACGAGTTCATCCCGCGCGTAGCCAAGCGTGGCGCGGAGATCATCGAGGTCCGCGGGCGCTCCTCG
GCAGCCTCCGCAGCAAGCGCCGCGCTGGACCACATGCGCGACTGGGTTAACGGCACCGCTGAGGGAGACTGGGTTTCCGT
CGCGCTGCCCTCCGATGGCTCCTATGGCATCCCGGAGGGGCTGGTGTTCTCCTTCCCATGCCGCTCCGTCGATGGCGAGT
GGGAGATCGTGCAGGGCCTCGAGATCAGCCCGGCGCAGCAGGAGCGCATCGACGCAAACATCAAGGAACTGCAGGAGGAA
AAGGCCGCCGTGGAGGAGGCTGGCCTGCTGTAG

Upstream 100 bases:

>100_bases
AGTGAGAGCGCAGTGAGAGTGCAGGGTGTCGGGCAGGTCATTACAGTAGGAAGAGGATCTAAGAAAACTCTCACAAACGC
ATTGCTTAAAGGAGCCCCGC

Downstream 100 bases:

>100_bases
CTTGCTGTAGCTTTGTATAGCTTTTTGAGCCAAAACTTTTTTCTGCATATCGCCACCCTGCCGACCGGTCGGGTGGTTTT
ATGTTAGGCATGACATCAGC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MTTNNQNAVTVTVTGAGGQIGYSLLFRIAKGEVFGDRVVNLRLLETEQGVQAARGVALELADCAFPLLGEVSITTELSEG
FKDANAVFLVGAKPRQKGEERADLLAANGKIFGPQGKAIAEYAADDVRVIVVGNPANTNAAIVAAHAEGLDPRRVTALTR
LDHNRGLAQVADKLGVAVRDLKNMTVWGNHSASQFPDVAELTLNGEKVADKLDAAWVNDEFIPRVAKRGAEIIEVRGRSS
AASAASAALDHMRDWVNGTAEGDWVSVALPSDGSYGIPEGLVFSFPCRSVDGEWEIVQGLEISPAQQERIDANIKELQEE
KAAVEEAGLL

Sequences:

>Translated_330_residues
MTTNNQNAVTVTVTGAGGQIGYSLLFRIAKGEVFGDRVVNLRLLETEQGVQAARGVALELADCAFPLLGEVSITTELSEG
FKDANAVFLVGAKPRQKGEERADLLAANGKIFGPQGKAIAEYAADDVRVIVVGNPANTNAAIVAAHAEGLDPRRVTALTR
LDHNRGLAQVADKLGVAVRDLKNMTVWGNHSASQFPDVAELTLNGEKVADKLDAAWVNDEFIPRVAKRGAEIIEVRGRSS
AASAASAALDHMRDWVNGTAEGDWVSVALPSDGSYGIPEGLVFSFPCRSVDGEWEIVQGLEISPAQQERIDANIKELQEE
KAAVEEAGLL
>Mature_329_residues
TTNNQNAVTVTVTGAGGQIGYSLLFRIAKGEVFGDRVVNLRLLETEQGVQAARGVALELADCAFPLLGEVSITTELSEGF
KDANAVFLVGAKPRQKGEERADLLAANGKIFGPQGKAIAEYAADDVRVIVVGNPANTNAAIVAAHAEGLDPRRVTALTRL
DHNRGLAQVADKLGVAVRDLKNMTVWGNHSASQFPDVAELTLNGEKVADKLDAAWVNDEFIPRVAKRGAEIIEVRGRSSA
ASAASAALDHMRDWVNGTAEGDWVSVALPSDGSYGIPEGLVFSFPCRSVDGEWEIVQGLEISPAQQERIDANIKELQEEK
AAVEEAGLL

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family

Homologues:

Organism=Homo sapiens, GI5174539, Length=325, Percent_Identity=47.3846153846154, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI89886456, Length=336, Percent_Identity=25.297619047619, Blast_Score=104, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17561064, Length=318, Percent_Identity=52.8301886792453, Blast_Score=295, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI32566798, Length=256, Percent_Identity=52.34375, Blast_Score=226, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI32566800, Length=169, Percent_Identity=52.0710059171598, Blast_Score=158, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24583394, Length=319, Percent_Identity=50.4702194357367, Blast_Score=296, Evalue=1e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MDH_CORJK (Q4JWV0)

Other databases:

- EMBL:   CR931997
- RefSeq:   YP_250325.1
- ProteinModelPortal:   Q4JWV0
- SMR:   Q4JWV0
- STRING:   Q4JWV0
- GeneID:   3433195
- GenomeReviews:   CR931997_GR
- KEGG:   cjk:jk0548
- NMPDR:   fig|306537.3.peg.544
- eggNOG:   COG0039
- HOGENOM:   HBG289884
- OMA:   MIIWGNH
- ProtClustDB:   PRK05442
- BioCyc:   CJEI306537:JK0548-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01517
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR010945
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR23382
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01759

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 35065; Mature: 34934

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00068 MDH

Important sites: ACT_SITE 190-190 BINDING 95-95 BINDING 101-101 BINDING 108-108 BINDING 115-115 BINDING 134-134 BINDING 165-165

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNNQNAVTVTVTGAGGQIGYSLLFRIAKGEVFGDRVVNLRLLETEQGVQAARGVALEL
CCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCHHHHCCCEEEE
ADCAFPLLGEVSITTELSEGFKDANAVFLVGAKPRQKGEERADLLAANGKIFGPQGKAIA
HHCCCCHHCCEEEEEHHHCCCCCCCEEEEEECCCHHCCHHHHHEEEECCEEECCCCCHHH
EYAADDVRVIVVGNPANTNAAIVAAHAEGLDPRRVTALTRLDHNRGLAQVADKLGVAVRD
HHCCCCEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LKNMTVWGNHSASQFPDVAELTLNGEKVADKLDAAWVNDEFIPRVAKRGAEIIEVRGRSS
HCCCEEECCCCHHHCCCCEEEEECCHHHHHHHHHHHCCCHHHHHHHHCCCEEEEECCCCC
AASAASAALDHMRDWVNGTAEGDWVSVALPSDGSYGIPEGLVFSFPCRSVDGEWEIVQGL
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCEEECCCCCCCCCEEEEECC
EISPAQQERIDANIKELQEEKAAVEEAGLL
CCCCCHHHHHCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTNNQNAVTVTVTGAGGQIGYSLLFRIAKGEVFGDRVVNLRLLETEQGVQAARGVALEL
CCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCHHHHCCCEEEE
ADCAFPLLGEVSITTELSEGFKDANAVFLVGAKPRQKGEERADLLAANGKIFGPQGKAIA
HHCCCCHHCCEEEEEHHHCCCCCCCEEEEEECCCHHCCHHHHHEEEECCEEECCCCCHHH
EYAADDVRVIVVGNPANTNAAIVAAHAEGLDPRRVTALTRLDHNRGLAQVADKLGVAVRD
HHCCCCEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LKNMTVWGNHSASQFPDVAELTLNGEKVADKLDAAWVNDEFIPRVAKRGAEIIEVRGRSS
HCCCEEECCCCHHHCCCCEEEEECCHHHHHHHHHHHCCCHHHHHHHHCCCEEEEECCCCC
AASAASAALDHMRDWVNGTAEGDWVSVALPSDGSYGIPEGLVFSFPCRSVDGEWEIVQGL
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCEEECCCCCCCCCEEEEECC
EISPAQQERIDANIKELQEEKAAVEEAGLL
CCCCCHHHHHCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA