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The map label for this gene is lipN [H]

Identifier: 68535621

GI number: 68535621

Start: 665177

End: 666274

Strand: Direct

Name: lipN [H]

Synonym: jk0549

Alternate gene names: 68535621

Gene position: 665177-666274 (Clockwise)

Preceding gene: 68535620

Following gene: 68535622

Centisome position: 27.01

GC content: 64.03

Gene sequence:

>1098_bases
ATGTTAGGCATGACATCAGCCACTGAACCCACAGCCCCGATCCACTCCCAACCACTCCAGCACTCACTTTTTAGCCAGCT
CTCCGCCCGCGCGGGGTGGCTGCTGCCCGCGATTCCCGAGCCAGTTCTGCGGATCTTTGGCTCCCGCACTAACTCCGACG
GTGATCGACTGGATCCCGACGTGCGTGCCTCCAACCTGGTCACCGACCTTATGCACCCGGCGGACTATTCCCAGCAGCCT
GTCGAGGAAACTCGTGCGGTAGTAGAAGACGCTGCGTACATGGCCGGTGGACACGTAACTCAGGTCGGCTCTGTGGTGGA
GCAGCAGATCCGGGGCGTGCGCGTGCGCCACTATCGACCCACCGGTGCGGCCTCGCCGGATGAAGATCTTCCCACTCTGG
TGTACTTCCACGGCGGTGGCTGGGTGGTCGGCTCCCTCGACTCGCACGATTCCACCTGTCGTTGGCTGTGCGAGGCCGCC
CGCGTGGCCGTGCTCTCCGTGGACTACCGCCTGGCCCCAGAGCACCCTTTCCCCGCCGCGCCTGACGACTGCTGTGAAGT
TGTTGATGCGGCGATGGCCGGCGAAGTTAAGGGCGTCAATAAGAATAGGGTGGCCGTCGGCGGCGATTCCGCGGGAGGCA
ACCTGGCCGCCGTGGTATGCCTGCGCCGTAAGCGCGAAGGACAGCAACAGCCGCTTGCGCAGCTGCTATTTGTGCCGGTT
ACGGATCTGTCCAGGCTGGATACACCCAGCCACAAGGAGTTCTCCAAGGGGTACTTCCTGACTCAGGCTCACATGAAGTG
GTACCGCGACCAGTACCTCACCAACGACTTGCAGCGGCTGGACCCGGACGTTTCGCCTCTGCTCGCCGAAGACGTCAGTG
GGGTGGCTCCGGCTTATGTCGCCGTCGCTGGTTTCGATCCGCTGCGCGACGAGGGCGTGGCCTATGCCGAGAAGCTGCGG
GAGGCAGGTGTGCCCGTTTCTTTCCGACGCCACCCCGGCCTGATCCACCCCTTCGTGAATTCTTCGGGTGTCTGGCGCAA
TTCCGCGCGGGCGTTGGACGAGGCTGCGGGTGTGCTGCGGGCGGCGCTCGAGATGTAA

Upstream 100 bases:

>100_bases
AGGAGGCTGGCCTGCTGTAGCTTGCTGTAGCTTTGTATAGCTTTTTGAGCCAAAACTTTTTTCTGCATATCGCCACCCTG
CCGACCGGTCGGGTGGTTTT

Downstream 100 bases:

>100_bases
ATGTCTAGTAGGAGAGTGGGGTACAGGCGAGGTGTAGACGGGTTGTAGACAGGTGGTATGCCGTGTCTGGCCCGTCTACT
AGACTTGGCAGACGTGAGTG

Product: putative lipase/esterase LipN

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MLGMTSATEPTAPIHSQPLQHSLFSQLSARAGWLLPAIPEPVLRIFGSRTNSDGDRLDPDVRASNLVTDLMHPADYSQQP
VEETRAVVEDAAYMAGGHVTQVGSVVEQQIRGVRVRHYRPTGAASPDEDLPTLVYFHGGGWVVGSLDSHDSTCRWLCEAA
RVAVLSVDYRLAPEHPFPAAPDDCCEVVDAAMAGEVKGVNKNRVAVGGDSAGGNLAAVVCLRRKREGQQQPLAQLLFVPV
TDLSRLDTPSHKEFSKGYFLTQAHMKWYRDQYLTNDLQRLDPDVSPLLAEDVSGVAPAYVAVAGFDPLRDEGVAYAEKLR
EAGVPVSFRRHPGLIHPFVNSSGVWRNSARALDEAAGVLRAALEM

Sequences:

>Translated_365_residues
MLGMTSATEPTAPIHSQPLQHSLFSQLSARAGWLLPAIPEPVLRIFGSRTNSDGDRLDPDVRASNLVTDLMHPADYSQQP
VEETRAVVEDAAYMAGGHVTQVGSVVEQQIRGVRVRHYRPTGAASPDEDLPTLVYFHGGGWVVGSLDSHDSTCRWLCEAA
RVAVLSVDYRLAPEHPFPAAPDDCCEVVDAAMAGEVKGVNKNRVAVGGDSAGGNLAAVVCLRRKREGQQQPLAQLLFVPV
TDLSRLDTPSHKEFSKGYFLTQAHMKWYRDQYLTNDLQRLDPDVSPLLAEDVSGVAPAYVAVAGFDPLRDEGVAYAEKLR
EAGVPVSFRRHPGLIHPFVNSSGVWRNSARALDEAAGVLRAALEM
>Mature_365_residues
MLGMTSATEPTAPIHSQPLQHSLFSQLSARAGWLLPAIPEPVLRIFGSRTNSDGDRLDPDVRASNLVTDLMHPADYSQQP
VEETRAVVEDAAYMAGGHVTQVGSVVEQQIRGVRVRHYRPTGAASPDEDLPTLVYFHGGGWVVGSLDSHDSTCRWLCEAA
RVAVLSVDYRLAPEHPFPAAPDDCCEVVDAAMAGEVKGVNKNRVAVGGDSAGGNLAAVVCLRRKREGQQQPLAQLLFVPV
TDLSRLDTPSHKEFSKGYFLTQAHMKWYRDQYLTNDLQRLDPDVSPLLAEDVSGVAPAYVAVAGFDPLRDEGVAYAEKLR
EAGVPVSFRRHPGLIHPFVNSSGVWRNSARALDEAAGVLRAALEM

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=180, Percent_Identity=33.8888888888889, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI157041239, Length=257, Percent_Identity=29.1828793774319, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI206597554, Length=284, Percent_Identity=28.169014084507, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI68051721, Length=339, Percent_Identity=25.3687315634218, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI61966717, Length=285, Percent_Identity=25.6140350877193, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI226423947, Length=347, Percent_Identity=24.4956772334294, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI21328446, Length=122, Percent_Identity=34.4262295081967, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1786682, Length=247, Percent_Identity=31.5789473684211, Blast_Score=113, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17567059, Length=264, Percent_Identity=35.2272727272727, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI72001146, Length=155, Percent_Identity=33.5483870967742, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17540028, Length=152, Percent_Identity=34.8684210526316, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71996133, Length=147, Percent_Identity=30.6122448979592, Blast_Score=85, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 39620; Mature: 39620

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGMTSATEPTAPIHSQPLQHSLFSQLSARAGWLLPAIPEPVLRIFGSRTNSDGDRLDPD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCCC
VRASNLVTDLMHPADYSQQPVEETRAVVEDAAYMAGGHVTQVGSVVEQQIRGVRVRHYRP
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECC
TGAASPDEDLPTLVYFHGGGWVVGSLDSHDSTCRWLCEAARVAVLSVDYRLAPEHPFPAA
CCCCCCCCCCCEEEEEECCCEEEECCCCCCHHHHHHHHHHHEEEEEECEEECCCCCCCCC
PDDCCEVVDAAMAGEVKGVNKNRVAVGGDSAGGNLAAVVCLRRKREGQQQPLAQLLFVPV
CHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCC
TDLSRLDTPSHKEFSKGYFLTQAHMKWYRDQYLTNDLQRLDPDVSPLLAEDVSGVAPAYV
HHHHHCCCCCCHHHHCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHH
AVAGFDPLRDEGVAYAEKLREAGVPVSFRRHPGLIHPFVNSSGVWRNSARALDEAAGVLR
EEECCCCHHHHHHHHHHHHHHCCCCEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHH
AALEM
HHHCC
>Mature Secondary Structure
MLGMTSATEPTAPIHSQPLQHSLFSQLSARAGWLLPAIPEPVLRIFGSRTNSDGDRLDPD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCCC
VRASNLVTDLMHPADYSQQPVEETRAVVEDAAYMAGGHVTQVGSVVEQQIRGVRVRHYRP
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECC
TGAASPDEDLPTLVYFHGGGWVVGSLDSHDSTCRWLCEAARVAVLSVDYRLAPEHPFPAA
CCCCCCCCCCCEEEEEECCCEEEECCCCCCHHHHHHHHHHHEEEEEECEEECCCCCCCCC
PDDCCEVVDAAMAGEVKGVNKNRVAVGGDSAGGNLAAVVCLRRKREGQQQPLAQLLFVPV
CHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCC
TDLSRLDTPSHKEFSKGYFLTQAHMKWYRDQYLTNDLQRLDPDVSPLLAEDVSGVAPAYV
HHHHHCCCCCCHHHHCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHH
AVAGFDPLRDEGVAYAEKLREAGVPVSFRRHPGLIHPFVNSSGVWRNSARALDEAAGVLR
EEECCCCHHHHHHHHHHHHHHCCCCEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHH
AALEM
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]