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The map label for this gene is cysD [H]

Identifier: 68535311

GI number: 68535311

Start: 303239

End: 304168

Strand: Direct

Name: cysD [H]

Synonym: jk0246

Alternate gene names: 68535311

Gene position: 303239-304168 (Clockwise)

Preceding gene: 68535310

Following gene: 68535312

Centisome position: 12.31

GC content: 65.91

Gene sequence:

>930_bases
ATGCGGACTTCACACATGAGCAATGAAAACACCACCGCCCTCTCGCCGCACCTGGCGAGCCTCGAGGCGGAAGCAATCGA
GATCCTGCGCGAAGTAGCAGGCCAGTTCGACCGCCCCGCGCTGCTGTTTTCCGGCGGCAAGGATTCCGTCGTCGTCCTGG
AGCTAGCGAAGCGCGCTTTCCACCCCGCACCGGTTCCCTTCGAACTGGTGCACGTGGATACCGGCCACAACTTCCCGGAG
GTCATCGAGTTCCGCGACAAGATCGCCGCGGACCCGAAGGTGACCCTGCACGTAGCCGCCGTCCAGGACTGGATCGACAG
CGGTGCCATCCAGGAACGCCCGGACGGTACCCGCAACCCACTGCAGACCGTGCCGCTGGTGGAGACCATCCAGAACCGCG
GCTACGACGCGGTGCTCGGCGGCGCTCGCCGCGACGAGGAGAAGGCCCGCGCCAAGGAGCGCGTGTTCTCCGTGCGCGAT
AGCTTCGGCGGCTGGAACCCGCGCCGCCAGCGACCCGAGCTGTGGGGCCTGTACAACGGCCGTCACCAGGTGGGCGAGAA
CATCCGCGTGTTCCCGATCTCCAACTGGACCGAGGCCGACGTGTGGGACTACATCCGCGACCGCAACGTCGAGGTCCCGG
CGATCTACTACTCCCACCAGCGCGAGGTCTTCAACCGCAACGGCATGTGGCTGACCCCGGGCGAGTGGGGTGGCCCGAAG
GAAGGCGAGGAGCTGGTCGAAAAGACCGTGCGCTACCGCACCGTCGGCGACATGAGCTGCACCGGTGCCGTCCTTTCCAC
CGCCACGACCATCGAGGACGTGATCGACGAAATCCGCCAGTCCACCACCACCGAGCGCGGCGCCACCCGTGCCGACGACA
AGCTCAGTGAGTCCTCCATGGAGGATCGCAAGAAGGAAGGTTACTTCTGA

Upstream 100 bases:

>100_bases
AGCAGGGCTACCCGTCGATCGGCTGCGAAACCTGCACCTTGCCAGTTGCCCCAGGACAGGACCCCCGATCCGGCCGCTGG
GCCGGCTCCAACAAGACAGA

Downstream 100 bases:

>100_bases
TGTCCACCAACCTGCCGACCCTCCGCCTGTGCACCGCCGGCTCCGTCGACGACGGCAAGTCCACCTTCGTGGGCCGCTTG
CTGCACGATACGAAGTCCAT

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Mitomycin biosynthesis protein V; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MRTSHMSNENTTALSPHLASLEAEAIEILREVAGQFDRPALLFSGGKDSVVVLELAKRAFHPAPVPFELVHVDTGHNFPE
VIEFRDKIAADPKVTLHVAAVQDWIDSGAIQERPDGTRNPLQTVPLVETIQNRGYDAVLGGARRDEEKARAKERVFSVRD
SFGGWNPRRQRPELWGLYNGRHQVGENIRVFPISNWTEADVWDYIRDRNVEVPAIYYSHQREVFNRNGMWLTPGEWGGPK
EGEELVEKTVRYRTVGDMSCTGAVLSTATTIEDVIDEIRQSTTTERGATRADDKLSESSMEDRKKEGYF

Sequences:

>Translated_309_residues
MRTSHMSNENTTALSPHLASLEAEAIEILREVAGQFDRPALLFSGGKDSVVVLELAKRAFHPAPVPFELVHVDTGHNFPE
VIEFRDKIAADPKVTLHVAAVQDWIDSGAIQERPDGTRNPLQTVPLVETIQNRGYDAVLGGARRDEEKARAKERVFSVRD
SFGGWNPRRQRPELWGLYNGRHQVGENIRVFPISNWTEADVWDYIRDRNVEVPAIYYSHQREVFNRNGMWLTPGEWGGPK
EGEELVEKTVRYRTVGDMSCTGAVLSTATTIEDVIDEIRQSTTTERGATRADDKLSESSMEDRKKEGYF
>Mature_309_residues
MRTSHMSNENTTALSPHLASLEAEAIEILREVAGQFDRPALLFSGGKDSVVVLELAKRAFHPAPVPFELVHVDTGHNFPE
VIEFRDKIAADPKVTLHVAAVQDWIDSGAIQERPDGTRNPLQTVPLVETIQNRGYDAVLGGARRDEEKARAKERVFSVRD
SFGGWNPRRQRPELWGLYNGRHQVGENIRVFPISNWTEADVWDYIRDRNVEVPAIYYSHQREVFNRNGMWLTPGEWGGPK
EGEELVEKTVRYRTVGDMSCTGAVLSTATTIEDVIDEIRQSTTTERGATRADDKLSESSMEDRKKEGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=297, Percent_Identity=45.1178451178451, Blast_Score=248, Evalue=4e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 34905; Mature: 34905

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSHMSNENTTALSPHLASLEAEAIEILREVAGQFDRPALLFSGGKDSVVVLELAKRAF
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEHHHHHHHHC
HPAPVPFELVHVDTGHNFPEVIEFRDKIAADPKVTLHVAAVQDWIDSGAIQERPDGTRNP
CCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCC
LQTVPLVETIQNRGYDAVLGGARRDEEKARAKERVFSVRDSFGGWNPRRQRPELWGLYNG
CHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEECCC
RHQVGENIRVFPISNWTEADVWDYIRDRNVEVPAIYYSHQREVFNRNGMWLTPGEWGGPK
HHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEHHHHHHHCCCCCEECCCCCCCCC
EGEELVEKTVRYRTVGDMSCTGAVLSTATTIEDVIDEIRQSTTTERGATRADDKLSESSM
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCHHHHHHHHH
EDRKKEGYF
HHHHHCCCC
>Mature Secondary Structure
MRTSHMSNENTTALSPHLASLEAEAIEILREVAGQFDRPALLFSGGKDSVVVLELAKRAF
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEHHHHHHHHC
HPAPVPFELVHVDTGHNFPEVIEFRDKIAADPKVTLHVAAVQDWIDSGAIQERPDGTRNP
CCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCC
LQTVPLVETIQNRGYDAVLGGARRDEEKARAKERVFSVRDSFGGWNPRRQRPELWGLYNG
CHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEECCC
RHQVGENIRVFPISNWTEADVWDYIRDRNVEVPAIYYSHQREVFNRNGMWLTPGEWGGPK
HHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEHHHHHHHCCCCCEECCCCCCCCC
EGEELVEKTVRYRTVGDMSCTGAVLSTATTIEDVIDEIRQSTTTERGATRADDKLSESSM
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCHHHHHHHHH
EDRKKEGYF
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10099135 [H]