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The map label for this gene is cysN [H]

Identifier: 68535312

GI number: 68535312

Start: 304168

End: 305427

Strand: Direct

Name: cysN [H]

Synonym: jk0247

Alternate gene names: 68535312

Gene position: 304168-305427 (Clockwise)

Preceding gene: 68535311

Following gene: 68535313

Centisome position: 12.35

GC content: 66.27

Gene sequence:

>1260_bases
ATGTCCACCAACCTGCCGACCCTCCGCCTGTGCACCGCCGGCTCCGTCGACGACGGCAAGTCCACCTTCGTGGGCCGCTT
GCTGCACGATACGAAGTCCATCCTGGCCGACCAGTATGAGTCCGTAGAGCGCGTTTCCAACGCCAAGGGCCTGGAGAACC
CGGATCTGTCGCTTCTGGTCGATGGTCTGCGTGCCGAGCGCGAACAGGGCATCACCATCGATGTGGCCTACCGTTACTTC
GCCACCGACAAGCGCAGCTTCATCCTGGCCGACACCCCGGGGCACGTGCAGTACACGCGCAACACCGTCACGGGCCTGTC
CACCTCGGAGCTCGTCATTGTTCTGGTTGACGCCCGCAACGGCGTGATCGAGCAGACCCGTCGCCACCTGACCGTGGCTG
CGATGATGAAGATCTCCCACGTTATCGTGGCCGTGAACAAGATCGATGCGGTCGATTTCAGCGAGCAGGTCTTCAACGAC
GTGAAATCCGACGTGGACGCGCTGGTCTCCGAGCTGGGCCTGGCGCACGTCGACGTGATCCCGACCTCCGCGCTGCTGGG
CGACAATGTCGTCGAGCGCAGCCAGAACACCCCCTGGTACACGGGTCCGTCCGTGCTGGAGATCCTGGAGACCACCGAGC
CGGCGAAGAACATCGCCGATGTGGAGCGCGGATTGCGTTTCCCGATCCAGATCGTCATCCGCGACCACGCCACCGACTAC
CGCGGCTACGCAGGCCGCATCACCGCGGGCAAGGTGTCCGTCGGCGATAAGGTGCTGGCCGATGGCCGCGAGGCGATCAT
CGAGGGGATCGACGGCCCGGCAGGGGAGCAGCAGACTGCCACCCGCGGCGAGTCAGTCTCCCTGCGCCTGGACCGCGACA
TCGACCTGTCCCGCGGGGACATCATCGCCGCCCCGGACCTGCCGGAGCCGCAGCAGTCCTTCACCGCGACGGTCTTCCAC
CTGTCCGAGTCCCCGGTGCGCCTGCGCAGCAATGTGCTGGTGCGCTACGGCACCGCGCTGGTGCGCGGTCGGGTGGACGA
GGTTATCCGTCGCGTGGACATCTCCGGCCAGAACCGCAGCGAGGAGGAGATCGCCCACGGTAGCGCCGACGAGCTGGCTC
TCAACGACATCGCGGAAGTGCGCGTGACGGTGGCCGAGCCGCTGCCCGTCGAAACCTACCGCCGCGGCGGTCGCGTGGGT
TCCCTGCTGCTGATCAACCCCGGTAGTGGCGACACCCTGACCGCAGGTCTGGTGAACTAA

Upstream 100 bases:

>100_bases
AAATCCGCCAGTCCACCACCACCGAGCGCGGCGCCACCCGTGCCGACGACAAGCTCAGTGAGTCCTCCATGGAGGATCGC
AAGAAGGAAGGTTACTTCTG

Downstream 100 bases:

>100_bases
CATGACTCGGCCGGCACCCATCATCTGCTTGGCGCATGGATCCCGCCACCCGCAGGCGGACCAGGCGGTCTTCGAGATCG
CCGAGCGCATTTCCGAAAGC

Product: sulfate adenylyltransferase subunit 1 / adenylylsulfate kinase

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSTNLPTLRLCTAGSVDDGKSTFVGRLLHDTKSILADQYESVERVSNAKGLENPDLSLLVDGLRAEREQGITIDVAYRYF
ATDKRSFILADTPGHVQYTRNTVTGLSTSELVIVLVDARNGVIEQTRRHLTVAAMMKISHVIVAVNKIDAVDFSEQVFND
VKSDVDALVSELGLAHVDVIPTSALLGDNVVERSQNTPWYTGPSVLEILETTEPAKNIADVERGLRFPIQIVIRDHATDY
RGYAGRITAGKVSVGDKVLADGREAIIEGIDGPAGEQQTATRGESVSLRLDRDIDLSRGDIIAAPDLPEPQQSFTATVFH
LSESPVRLRSNVLVRYGTALVRGRVDEVIRRVDISGQNRSEEEIAHGSADELALNDIAEVRVTVAEPLPVETYRRGGRVG
SLLLINPGSGDTLTAGLVN

Sequences:

>Translated_419_residues
MSTNLPTLRLCTAGSVDDGKSTFVGRLLHDTKSILADQYESVERVSNAKGLENPDLSLLVDGLRAEREQGITIDVAYRYF
ATDKRSFILADTPGHVQYTRNTVTGLSTSELVIVLVDARNGVIEQTRRHLTVAAMMKISHVIVAVNKIDAVDFSEQVFND
VKSDVDALVSELGLAHVDVIPTSALLGDNVVERSQNTPWYTGPSVLEILETTEPAKNIADVERGLRFPIQIVIRDHATDY
RGYAGRITAGKVSVGDKVLADGREAIIEGIDGPAGEQQTATRGESVSLRLDRDIDLSRGDIIAAPDLPEPQQSFTATVFH
LSESPVRLRSNVLVRYGTALVRGRVDEVIRRVDISGQNRSEEEIAHGSADELALNDIAEVRVTVAEPLPVETYRRGGRVG
SLLLINPGSGDTLTAGLVN
>Mature_418_residues
STNLPTLRLCTAGSVDDGKSTFVGRLLHDTKSILADQYESVERVSNAKGLENPDLSLLVDGLRAEREQGITIDVAYRYFA
TDKRSFILADTPGHVQYTRNTVTGLSTSELVIVLVDARNGVIEQTRRHLTVAAMMKISHVIVAVNKIDAVDFSEQVFNDV
KSDVDALVSELGLAHVDVIPTSALLGDNVVERSQNTPWYTGPSVLEILETTEPAKNIADVERGLRFPIQIVIRDHATDYR
GYAGRITAGKVSVGDKVLADGREAIIEGIDGPAGEQQTATRGESVSLRLDRDIDLSRGDIIAAPDLPEPQQSFTATVFHL
SESPVRLRSNVLVRYGTALVRGRVDEVIRRVDISGQNRSEEEIAHGSADELALNDIAEVRVTVAEPLPVETYRRGGRVGS
LLLINPGSGDTLTAGLVN

Specific function: May be the GTPase, regulating ATP sulfurylase activity [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI223555963, Length=434, Percent_Identity=29.7235023041475, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI5729864, Length=434, Percent_Identity=29.7235023041475, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI4503475, Length=426, Percent_Identity=26.5258215962441, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI194018522, Length=424, Percent_Identity=25.4716981132075, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI194097354, Length=424, Percent_Identity=25.4716981132075, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI194018520, Length=424, Percent_Identity=25.4716981132075, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI46094014, Length=424, Percent_Identity=25, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI4503471, Length=424, Percent_Identity=26.4150943396226, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI34147630, Length=335, Percent_Identity=27.7611940298507, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI157426893, Length=254, Percent_Identity=31.1023622047244, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1789108, Length=418, Percent_Identity=45.2153110047847, Blast_Score=332, Evalue=2e-92,
Organism=Escherichia coli, GI1789737, Length=327, Percent_Identity=24.7706422018349, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790412, Length=327, Percent_Identity=24.7706422018349, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI2367247, Length=347, Percent_Identity=24.4956772334294, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17552884, Length=265, Percent_Identity=31.6981132075472, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17569207, Length=265, Percent_Identity=31.6981132075472, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI32566629, Length=432, Percent_Identity=25.462962962963, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI115532067, Length=262, Percent_Identity=29.7709923664122, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI115532065, Length=262, Percent_Identity=29.7709923664122, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32566303, Length=264, Percent_Identity=28.4090909090909, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI32566301, Length=136, Percent_Identity=32.3529411764706, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17556456, Length=207, Percent_Identity=28.0193236714976, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI25141371, Length=141, Percent_Identity=27.6595744680851, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322937, Length=453, Percent_Identity=25.8278145695364, Blast_Score=149, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6325337, Length=421, Percent_Identity=27.5534441805226, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6319594, Length=421, Percent_Identity=27.5534441805226, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320377, Length=427, Percent_Identity=27.4004683840749, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6324761, Length=415, Percent_Identity=26.0240963855422, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6323320, Length=183, Percent_Identity=33.879781420765, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24652838, Length=427, Percent_Identity=27.1662763466042, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI17137572, Length=427, Percent_Identity=27.1662763466042, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI45553807, Length=271, Percent_Identity=30.6273062730627, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI45553816, Length=271, Percent_Identity=30.6273062730627, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24651721, Length=271, Percent_Identity=30.6273062730627, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI17864154, Length=271, Percent_Identity=30.6273062730627, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI45550900, Length=435, Percent_Identity=25.9770114942529, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI17137380, Length=256, Percent_Identity=28.515625, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI281363316, Length=335, Percent_Identity=25.3731343283582, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17864358, Length=335, Percent_Identity=25.3731343283582, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19921738, Length=209, Percent_Identity=27.2727272727273, Blast_Score=89, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 45453; Mature: 45322

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00018 EF_HAND_1 ; PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTNLPTLRLCTAGSVDDGKSTFVGRLLHDTKSILADQYESVERVSNAKGLENPDLSLLV
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
DGLRAEREQGITIDVAYRYFATDKRSFILADTPGHVQYTRNTVTGLSTSELVIVLVDARN
HHHHHHHHCCCEEEEEEEEEECCCCCEEEECCCCCEEEECCEECCCCCCCEEEEEEECCC
GVIEQTRRHLTVAAMMKISHVIVAVNKIDAVDFSEQVFNDVKSDVDALVSELGLAHVDVI
CHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEC
PTSALLGDNVVERSQNTPWYTGPSVLEILETTEPAKNIADVERGLRFPIQIVIRDHATDY
CCHHHHCCHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEECCCCCC
RGYAGRITAGKVSVGDKVLADGREAIIEGIDGPAGEQQTATRGESVSLRLDRDIDLSRGD
CCCCCEEEECEECCCCHHHHCCHHHHHHCCCCCCCCCHHHCCCCEEEEEECCCCCCCCCC
IIAAPDLPEPQQSFTATVFHLSESPVRLRSNVLVRYGTALVRGRVDEVIRRVDISGQNRS
EEECCCCCCCHHHHEEEEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
EEEIAHGSADELALNDIAEVRVTVAEPLPVETYRRGGRVGSLLLINPGSGDTLTAGLVN
HHHHHCCCCCHHHHHHHHHEEEEECCCCCHHHHHCCCCEEEEEEEECCCCCEEECCCCC
>Mature Secondary Structure 
STNLPTLRLCTAGSVDDGKSTFVGRLLHDTKSILADQYESVERVSNAKGLENPDLSLLV
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
DGLRAEREQGITIDVAYRYFATDKRSFILADTPGHVQYTRNTVTGLSTSELVIVLVDARN
HHHHHHHHCCCEEEEEEEEEECCCCCEEEECCCCCEEEECCEECCCCCCCEEEEEEECCC
GVIEQTRRHLTVAAMMKISHVIVAVNKIDAVDFSEQVFNDVKSDVDALVSELGLAHVDVI
CHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEC
PTSALLGDNVVERSQNTPWYTGPSVLEILETTEPAKNIADVERGLRFPIQIVIRDHATDY
CCHHHHCCHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEECCCCCC
RGYAGRITAGKVSVGDKVLADGREAIIEGIDGPAGEQQTATRGESVSLRLDRDIDLSRGD
CCCCCEEEECEECCCCHHHHCCHHHHHHCCCCCCCCCHHHCCCCEEEEEECCCCCCCCCC
IIAAPDLPEPQQSFTATVFHLSESPVRLRSNVLVRYGTALVRGRVDEVIRRVDISGQNRS
EEECCCCCCCHHHHEEEEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
EEEIAHGSADELALNDIAEVRVTVAEPLPVETYRRGGRVGSLLLINPGSGDTLTAGLVN
HHHHHCCCCCHHHHHHHHHEEEEECCCCCHHHHHCCCCEEEEEEEECCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA