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The map label for this gene is pilR [H]

Identifier: 66767389

GI number: 66767389

Start: 1277218

End: 1278621

Strand: Reverse

Name: pilR [H]

Synonym: XC_1061

Alternate gene names: 66767389

Gene position: 1278621-1277218 (Counterclockwise)

Preceding gene: 66767390

Following gene: 77761128

Centisome position: 24.83

GC content: 66.1

Gene sequence:

>1404_bases
GTGCGCAACATGAACGAAACGAAAAGTGCCCTGGTCGTCGATGACGAGCGTGACATCCGCGAATTGCTTGTTCTCACCCT
GGGCCGCATGGGGCTGCGCATCAGCACCGCCGCCAACCTGGCCGAAGCACGCGAATTGCTGGCCAGCAACCCGTACGACC
TGTGCCTGACCGACATGCGGTTGCCCGACGGCAACGGCATCGAGCTGGTGACCGAGATCGCGCGCCAATACCCGCAGACG
CCGGTGGCCATGATCACCGCGTTCGGCAGCATGGACCTGGCGGTGGAAGCGCTGAAAGCCGGCGCGTTCGACTTCGTCAG
CAAGCCGGTGGACATCAGCGTGCTGCGCGGCCTGGTCAAGCACGCACTGGAATTGAACAACCGCGACCGGCCGGCGCCGC
CACCGCCTCCGCTGGAACAGGCCAGCCGCCTGCTCGGCGATTCGACCGCCATGGAGAGCCTGCGCTCCACCATCGGCAAG
GTCGCGCGCAGCCAGGCGCCGGTCTACATCGTTGGCGAATCCGGCGTGGGCAAGGAACTGGTGGCCCGCACCATCCACGA
GCAGGGCGCGCGCGCGGCCGGGCCGTTCATTCCGGTCAACTGCGGCGCGATCCCCGCCGAGCTGATGGAGAGCGAGTTCT
TCGGCCATAAGAAGGGCAGCTTTACCGGCGCGCATGCCGACAAACCGGGTCTGTTTCAGGCCGCGCATGGCGGCACGCTG
TTTCTGGACGAAGTGGCCGAGCTGCCGCTGCAGATGCAGGTCAAGCTGCTGCGCGCCATCCAGGAAAAATCAGTGCGCCC
GGTCGGCGCCTCGGGCGAGACGCTGGTGGACGTGCGCATTCTGTCGGCCACACACAAGGACCTGGGCGACCTGGTCTCCG
ACGGCCGCTTTCGTCACGATCTGTATTACCGCATCAACGTGATCGAGCTGCGTGTGCCACCGCTGCGCGAGCGCAGTGGC
GACCTGCCGCAACTGGCCGCCGCCATCATTGCGCGCCTGGCCCGCAGCCATGGCCGCCCGATCCCGCTGCTGACCCAGTC
AGCCCTGGATGCATTGGATACTTACGGCTTTCCGGGCAACGTGCGCGAACTGGAAAACATCCTCGAACGCGCCCTGGCCC
TGGCCGAAGACGACCAGATCAGCGCCAGCGATCTGCGCCTACCCGCCCACGGCGGCCATCGCCTTGCCGCCAGCCCCGGC
AGCGCCGCCATCGAACCGCGCGAAGCGGTCGTCGACATCGATCCGGCCTCCTCTGCCCTGCCCTCCTACATCGAGCAACT
GGAACGCGCCGCGATCCAGAAGGCGCTGGAAGAAAACCGCTGGAACAAGACCAAGACCGCCGCCCAGCTCGGCATCACGT
TTCGTGCGTTGCGCTACAAGCTGAAGAAATTGGGGATGGAGTAG

Upstream 100 bases:

>100_bases
AACGTGGGACGAACATCGGTAGCAGCCCCGCACGCCCAACACATGACGCTTCAGGTCCAGCAGCGCCGGTAATTGTCGAC
TGTCATCACGCTGGGCTATC

Downstream 100 bases:

>100_bases
AGGGGCCGGGAATTGGGAGTGGGGAATCGGGAATCGTAAAAGCGGGTTGGTTGGTTTCGCTTGCTCTGGCCCCGCCGTCC
ATCGCTACTGGATGCGGCAT

Product: two-component system regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MRNMNETKSALVVDDERDIRELLVLTLGRMGLRISTAANLAEARELLASNPYDLCLTDMRLPDGNGIELVTEIARQYPQT
PVAMITAFGSMDLAVEALKAGAFDFVSKPVDISVLRGLVKHALELNNRDRPAPPPPPLEQASRLLGDSTAMESLRSTIGK
VARSQAPVYIVGESGVGKELVARTIHEQGARAAGPFIPVNCGAIPAELMESEFFGHKKGSFTGAHADKPGLFQAAHGGTL
FLDEVAELPLQMQVKLLRAIQEKSVRPVGASGETLVDVRILSATHKDLGDLVSDGRFRHDLYYRINVIELRVPPLRERSG
DLPQLAAAIIARLARSHGRPIPLLTQSALDALDTYGFPGNVRELENILERALALAEDDQISASDLRLPAHGGHRLAASPG
SAAIEPREAVVDIDPASSALPSYIEQLERAAIQKALEENRWNKTKTAAQLGITFRALRYKLKKLGME

Sequences:

>Translated_467_residues
MRNMNETKSALVVDDERDIRELLVLTLGRMGLRISTAANLAEARELLASNPYDLCLTDMRLPDGNGIELVTEIARQYPQT
PVAMITAFGSMDLAVEALKAGAFDFVSKPVDISVLRGLVKHALELNNRDRPAPPPPPLEQASRLLGDSTAMESLRSTIGK
VARSQAPVYIVGESGVGKELVARTIHEQGARAAGPFIPVNCGAIPAELMESEFFGHKKGSFTGAHADKPGLFQAAHGGTL
FLDEVAELPLQMQVKLLRAIQEKSVRPVGASGETLVDVRILSATHKDLGDLVSDGRFRHDLYYRINVIELRVPPLRERSG
DLPQLAAAIIARLARSHGRPIPLLTQSALDALDTYGFPGNVRELENILERALALAEDDQISASDLRLPAHGGHRLAASPG
SAAIEPREAVVDIDPASSALPSYIEQLERAAIQKALEENRWNKTKTAAQLGITFRALRYKLKKLGME
>Mature_467_residues
MRNMNETKSALVVDDERDIRELLVLTLGRMGLRISTAANLAEARELLASNPYDLCLTDMRLPDGNGIELVTEIARQYPQT
PVAMITAFGSMDLAVEALKAGAFDFVSKPVDISVLRGLVKHALELNNRDRPAPPPPPLEQASRLLGDSTAMESLRSTIGK
VARSQAPVYIVGESGVGKELVARTIHEQGARAAGPFIPVNCGAIPAELMESEFFGHKKGSFTGAHADKPGLFQAAHGGTL
FLDEVAELPLQMQVKLLRAIQEKSVRPVGASGETLVDVRILSATHKDLGDLVSDGRFRHDLYYRINVIELRVPPLRERSG
DLPQLAAAIIARLARSHGRPIPLLTQSALDALDTYGFPGNVRELENILERALALAEDDQISASDLRLPAHGGHRLAASPG
SAAIEPREAVVDIDPASSALPSYIEQLERAAIQKALEENRWNKTKTAAQLGITFRALRYKLKKLGME

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=472, Percent_Identity=38.5593220338983, Blast_Score=329, Evalue=3e-91,
Organism=Escherichia coli, GI1790437, Length=453, Percent_Identity=41.280353200883, Blast_Score=311, Evalue=4e-86,
Organism=Escherichia coli, GI1790299, Length=466, Percent_Identity=38.8412017167382, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1788905, Length=456, Percent_Identity=37.719298245614, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI1789233, Length=333, Percent_Identity=40.5405405405405, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI87082117, Length=238, Percent_Identity=46.6386554621849, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI1789087, Length=247, Percent_Identity=45.3441295546559, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI87082152, Length=333, Percent_Identity=39.9399399399399, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI1786524, Length=294, Percent_Identity=40.4761904761905, Blast_Score=205, Evalue=6e-54,
Organism=Escherichia coli, GI87081872, Length=326, Percent_Identity=35.5828220858896, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1787583, Length=325, Percent_Identity=35.3846153846154, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1789828, Length=233, Percent_Identity=37.7682403433476, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI87081858, Length=309, Percent_Identity=31.3915857605178, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1786911, Length=142, Percent_Identity=33.8028169014084, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787487, Length=145, Percent_Identity=33.7931034482759, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50672; Mature: 50672

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNMNETKSALVVDDERDIRELLVLTLGRMGLRISTAANLAEARELLASNPYDLCLTDMR
CCCCCCCCCEEEECCHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHCCCCCEEEEECC
LPDGNGIELVTEIARQYPQTPVAMITAFGSMDLAVEALKAGAFDFVSKPVDISVLRGLVK
CCCCCCHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHCCCHHHHCCCCCHHHHHHHHH
HALELNNRDRPAPPPPPLEQASRLLGDSTAMESLRSTIGKVARSQAPVYIVGESGVGKEL
HHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHH
VARTIHEQGARAAGPFIPVNCGAIPAELMESEFFGHKKGSFTGAHADKPGLFQAAHGGTL
HHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCEE
FLDEVAELPLQMQVKLLRAIQEKSVRPVGASGETLVDVRILSATHKDLGDLVSDGRFRHD
EHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHCCCEEEE
LYYRINVIELRVPPLRERSGDLPQLAAAIIARLARSHGRPIPLLTQSALDALDTYGFPGN
EEEEEEEEEEECCCCHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
VRELENILERALALAEDDQISASDLRLPAHGGHRLAASPGSAAIEPREAVVDIDPASSAL
HHHHHHHHHHHHHHCCCCCCCHHHEECCCCCCCEEECCCCCCCCCCCHHEEECCCCHHHH
PSYIEQLERAAIQKALEENRWNKTKTAAQLGITFRALRYKLKKLGME
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRNMNETKSALVVDDERDIRELLVLTLGRMGLRISTAANLAEARELLASNPYDLCLTDMR
CCCCCCCCCEEEECCHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHCCCCCEEEEECC
LPDGNGIELVTEIARQYPQTPVAMITAFGSMDLAVEALKAGAFDFVSKPVDISVLRGLVK
CCCCCCHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHCCCHHHHCCCCCHHHHHHHHH
HALELNNRDRPAPPPPPLEQASRLLGDSTAMESLRSTIGKVARSQAPVYIVGESGVGKEL
HHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHH
VARTIHEQGARAAGPFIPVNCGAIPAELMESEFFGHKKGSFTGAHADKPGLFQAAHGGTL
HHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCEE
FLDEVAELPLQMQVKLLRAIQEKSVRPVGASGETLVDVRILSATHKDLGDLVSDGRFRHD
EHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHCCCEEEE
LYYRINVIELRVPPLRERSGDLPQLAAAIIARLARSHGRPIPLLTQSALDALDTYGFPGN
EEEEEEEEEEECCCCHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
VRELENILERALALAEDDQISASDLRLPAHGGHRLAASPGSAAIEPREAVVDIDPASSAL
HHHHHHHHHHHHHHCCCCCCCHHHEECCCCCCCEEECCCCCCCCCCCHHEEECCCCHHHH
PSYIEQLERAAIQKALEENRWNKTKTAAQLGITFRALRYKLKKLGME
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]