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The map label for this gene is pilS [H]

Identifier: 66767390

GI number: 66767390

Start: 1278821

End: 1280431

Strand: Reverse

Name: pilS [H]

Synonym: XC_1062

Alternate gene names: 66767390

Gene position: 1280431-1278821 (Counterclockwise)

Preceding gene: 66767396

Following gene: 66767389

Centisome position: 24.87

GC content: 69.03

Gene sequence:

>1611_bases
GTGGCATCCAGCGTATCGCTCATCGACCGGATCCAGTCGGCGCCGCAGCGCGAGCTGTATTTCTTCTCGCTGTACCGCGT
GCTGGTGGCCGGCCTGATCGCCGCGCTGGTGTTCAGCCCGTTGTCCGACGCCATCCCCGAGCCGCGCTACCCGCGGTTGG
CCGCCGGGGTGGCGATCGGCTATCTGTCGATGGCCATCCCGCTGCTGTTCTGGGGCCGCAGCGAGCGCCGCCTCACCGCC
ACCGTGCTGTGCGCGGTGCTGGCCGACATCCTGGCCGCGACCCTGGCCACGCACGCCCTGCCCGGCGCCAGCGACGGTAT
CGCGATGATGCTGCTGTTCAACGTGGCGGCCGGCTCGATCCTGCTGCGGCTGCGCTACGGCATGAGTGTCGCGGTGGTGG
CCAGCACCGCGATCGTGCTGGAGTACCTGTGGACGCTACTGGAAGGCGGCACCACCCGCCCGGTGGCCGAGCTGGCCATG
TTCGCCACCAGCTACGTGGCGGTGGCCTACATCTGCCAGCAGATCGCCTCGCGCGCGCGCAGCAACCAGGCGCTGGCCGA
ACAACGCGGCGCGCAGGTCGCCAACCTTTACGAAATCAACGAGCTGATCATCCGCCGCATGCGCACCGGCGTGCTGGTGG
TCGATACCCACAACCGCATCACCCTGGCCAACGAAGCCGCCCTGGCACTGCTCGGCGACGGCGACCAGCGCAACGCCCCG
GCCGACCTGGCCCTGGCCGCGCTCACCCCGGAACTGGCGCGCCGGCTGCAGCGCTGGCGCAGCGGCTGGCGCCAGGAAGA
ATCCCCGCTGCAGCTGGGCGCGGACCGCCCCGAAGTGCAGCCGCGCTTCGTGCGCCTGCTCGCCGACAGCGACCTGGTGC
TGATCTTCCTGGACGATGCCAGCGTGGTCTCGCGGCGCGCCGAATCGCTGACCCTCTCGGCCATGGGCCGGTTCTCGGCC
AGCCTGGCCCACGAGATCCGCAACCCGCTGGCGGCCATCAACTACGCCTCGCAGCTGCTGGAAGAATCGCCGGACATCGG
CGATGCCGACCGCCGCCTGCTGCAGATCATCCACCAGCAATGCCAGCGCACCAACGGCATCGTCGAAAGCGTGCTCGGCC
TGGCCCGGCGCGAGCGCGCCAGCCCGGAAAACCTGGACCTGGCCGCGTTCGTGCGCCGCTTCGTCGTGGAATACCGGCAG
ACCTTGTCGATGGAGACCGACATCCTGGAAGCCAGCATCGAGGGCGCCTCGGTGCACGCCTTGATCGACCCCAAGCACCT
GCACCAGGTCCTGACCGCGCTGGTACACAACGCACTCAAGTACGGCCGCATCCTGGACGAGCCGGCACGGGTGAAGCTGC
GGGTGGAGCGGCGCGAGCGCATGGTGGCCATCGACGTCATCGACCGTGGCCCCGGCATTCCTGAAACCGTGGCAGCGCAG
CTGTTCCGACCGTTCTACACCACCTCCGAACACGGCACCGGGCTCGGCCTGTATATCGCCCAGGAACTGTGCCGCGCCAA
CCAGGCGCAACTGGATTACGTCTCCGTCCCCGGCGGCGGCGCGTGTTTCCGCATCCTGCTACAGGGCCCGAATGGGCTAT
TCGGCAAGTAG

Upstream 100 bases:

>100_bases
CCAGATCTGTGCGCTTGCGCAATGCGCGAATGCATGCGCAAGCGGCCGTGGGCGGCGCAGCTGCCTATACTCGCGGCTCG
TTTCAGCAGGGGGTTCAGGC

Downstream 100 bases:

>100_bases
GCGAGCAGAGCAAATCTGCGCAGGCCGGCTGTCCTGCCCGGGCAGCAATCGGCTCGCAATCAGCAGCGATCTCCGCGCGC
GCGAGGCCTTGTGGCACCGA

Product: two-component system sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MASSVSLIDRIQSAPQRELYFFSLYRVLVAGLIAALVFSPLSDAIPEPRYPRLAAGVAIGYLSMAIPLLFWGRSERRLTA
TVLCAVLADILAATLATHALPGASDGIAMMLLFNVAAGSILLRLRYGMSVAVVASTAIVLEYLWTLLEGGTTRPVAELAM
FATSYVAVAYICQQIASRARSNQALAEQRGAQVANLYEINELIIRRMRTGVLVVDTHNRITLANEAALALLGDGDQRNAP
ADLALAALTPELARRLQRWRSGWRQEESPLQLGADRPEVQPRFVRLLADSDLVLIFLDDASVVSRRAESLTLSAMGRFSA
SLAHEIRNPLAAINYASQLLEESPDIGDADRRLLQIIHQQCQRTNGIVESVLGLARRERASPENLDLAAFVRRFVVEYRQ
TLSMETDILEASIEGASVHALIDPKHLHQVLTALVHNALKYGRILDEPARVKLRVERRERMVAIDVIDRGPGIPETVAAQ
LFRPFYTTSEHGTGLGLYIAQELCRANQAQLDYVSVPGGGACFRILLQGPNGLFGK

Sequences:

>Translated_536_residues
MASSVSLIDRIQSAPQRELYFFSLYRVLVAGLIAALVFSPLSDAIPEPRYPRLAAGVAIGYLSMAIPLLFWGRSERRLTA
TVLCAVLADILAATLATHALPGASDGIAMMLLFNVAAGSILLRLRYGMSVAVVASTAIVLEYLWTLLEGGTTRPVAELAM
FATSYVAVAYICQQIASRARSNQALAEQRGAQVANLYEINELIIRRMRTGVLVVDTHNRITLANEAALALLGDGDQRNAP
ADLALAALTPELARRLQRWRSGWRQEESPLQLGADRPEVQPRFVRLLADSDLVLIFLDDASVVSRRAESLTLSAMGRFSA
SLAHEIRNPLAAINYASQLLEESPDIGDADRRLLQIIHQQCQRTNGIVESVLGLARRERASPENLDLAAFVRRFVVEYRQ
TLSMETDILEASIEGASVHALIDPKHLHQVLTALVHNALKYGRILDEPARVKLRVERRERMVAIDVIDRGPGIPETVAAQ
LFRPFYTTSEHGTGLGLYIAQELCRANQAQLDYVSVPGGGACFRILLQGPNGLFGK
>Mature_535_residues
ASSVSLIDRIQSAPQRELYFFSLYRVLVAGLIAALVFSPLSDAIPEPRYPRLAAGVAIGYLSMAIPLLFWGRSERRLTAT
VLCAVLADILAATLATHALPGASDGIAMMLLFNVAAGSILLRLRYGMSVAVVASTAIVLEYLWTLLEGGTTRPVAELAMF
ATSYVAVAYICQQIASRARSNQALAEQRGAQVANLYEINELIIRRMRTGVLVVDTHNRITLANEAALALLGDGDQRNAPA
DLALAALTPELARRLQRWRSGWRQEESPLQLGADRPEVQPRFVRLLADSDLVLIFLDDASVVSRRAESLTLSAMGRFSAS
LAHEIRNPLAAINYASQLLEESPDIGDADRRLLQIIHQQCQRTNGIVESVLGLARRERASPENLDLAAFVRRFVVEYRQT
LSMETDILEASIEGASVHALIDPKHLHQVLTALVHNALKYGRILDEPARVKLRVERRERMVAIDVIDRGPGIPETVAAQL
FRPFYTTSEHGTGLGLYIAQELCRANQAQLDYVSVPGGGACFRILLQGPNGLFGK

Specific function: Member of the two-component regulatory system pilS/pilR involved in the regulation of the expression of type 4 fimbriae. PilS may function as a membrane-associated protein kinase that phosphorylates pilR in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=376, Percent_Identity=26.063829787234, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI87082128, Length=222, Percent_Identity=28.8288288288288, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790436, Length=227, Percent_Identity=26.8722466960352, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1790861, Length=211, Percent_Identity=27.9620853080569, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1786600, Length=223, Percent_Identity=28.2511210762332, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1790300, Length=208, Percent_Identity=31.7307692307692, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI87081816, Length=233, Percent_Identity=30.0429184549356, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788713, Length=229, Percent_Identity=26.2008733624454, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1789149, Length=241, Percent_Identity=26.5560165975104, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1789808, Length=223, Percent_Identity=27.3542600896861, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 58703; Mature: 58571

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSVSLIDRIQSAPQRELYFFSLYRVLVAGLIAALVFSPLSDAIPEPRYPRLAAGVAIG
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
YLSMAIPLLFWGRSERRLTATVLCAVLADILAATLATHALPGASDGIAMMLLFNVAAGSI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
LLRLRYGMSVAVVASTAIVLEYLWTLLEGGTTRPVAELAMFATSYVAVAYICQQIASRAR
HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNQALAEQRGAQVANLYEINELIIRRMRTGVLVVDTHNRITLANEAALALLGDGDQRNAP
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEECCCEEEEECCCCCCCCC
ADLALAALTPELARRLQRWRSGWRQEESPLQLGADRPEVQPRFVRLLADSDLVLIFLDDA
HHHHHHHHCHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCHHHHHHHCCCCEEEEEECCH
SVVSRRAESLTLSAMGRFSASLAHEIRNPLAAINYASQLLEESPDIGDADRRLLQIIHQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
CQRTNGIVESVLGLARRERASPENLDLAAFVRRFVVEYRQTLSMETDILEASIEGASVHA
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
LIDPKHLHQVLTALVHNALKYGRILDEPARVKLRVERRERMVAIDVIDRGPGIPETVAAQ
EECHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEHHHHCEEEEEEECCCCCCCHHHHHH
LFRPFYTTSEHGTGLGLYIAQELCRANQAQLDYVSVPGGGACFRILLQGPNGLFGK
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure 
ASSVSLIDRIQSAPQRELYFFSLYRVLVAGLIAALVFSPLSDAIPEPRYPRLAAGVAIG
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
YLSMAIPLLFWGRSERRLTATVLCAVLADILAATLATHALPGASDGIAMMLLFNVAAGSI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
LLRLRYGMSVAVVASTAIVLEYLWTLLEGGTTRPVAELAMFATSYVAVAYICQQIASRAR
HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNQALAEQRGAQVANLYEINELIIRRMRTGVLVVDTHNRITLANEAALALLGDGDQRNAP
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEECCCEEEEECCCCCCCCC
ADLALAALTPELARRLQRWRSGWRQEESPLQLGADRPEVQPRFVRLLADSDLVLIFLDDA
HHHHHHHHCHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCHHHHHHHCCCCEEEEEECCH
SVVSRRAESLTLSAMGRFSASLAHEIRNPLAAINYASQLLEESPDIGDADRRLLQIIHQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
CQRTNGIVESVLGLARRERASPENLDLAAFVRRFVVEYRQTLSMETDILEASIEGASVHA
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
LIDPKHLHQVLTALVHNALKYGRILDEPARVKLRVERRERMVAIDVIDRGPGIPETVAAQ
EECHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEHHHHCEEEEEEECCCCCCCHHHHHH
LFRPFYTTSEHGTGLGLYIAQELCRANQAQLDYVSVPGGGACFRILLQGPNGLFGK
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8097014; 10984043 [H]