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The map label for this gene is gudD [H]

Identifier: 66767283

GI number: 66767283

Start: 1142266

End: 1143621

Strand: Reverse

Name: gudD [H]

Synonym: XC_0951

Alternate gene names: 66767283

Gene position: 1143621-1142266 (Counterclockwise)

Preceding gene: 66767284

Following gene: 66767282

Centisome position: 22.21

GC content: 65.34

Gene sequence:

>1356_bases
ATGAACATCCATTCTTCAAGTGCGCGCAGCAGCGCGACACCACGCATCACCGACGTGCGCGTGGTGCCGGTCGCCGGGCA
GGACAGCATGCTGCTGAACCTGAGTGGCGCGCACGCACCGTACTTCACCCGCAACGTGCTGGTGCTGCACGATTCCTCCG
GGCGCATCGGCGTGGGCGAAGTGCCGGGCGGCGAGGCGATTGCCGCGCTGTTGCGCGAGGCCGCCGGGTTGATCCTTGAC
CGGCCCATCGCCGACTACCAGCGCATCCTCAATCAGGTGCGCGCAACCTTTGCCGACCGCGACAGTGCGGGCCGTGGCCT
GCAGACCTTCGATCTGCGCATCACCATCCATGCGGTCACCGCGATCGAGTCGGCATTGCTGGACCTGCTTGGGCAATTTC
TGGAGCAGCCGGTGGCCGCGTTACTGGGCGAAGGCCAGCAACGCGACGCGGTGGATGTGCTTGGCTATCTGTTCTTCATC
GGCGACCGCCGCAAGGCCAGCGTGGCCTACCGCGATGGCAGCGGCGCGCGTGATCGCTGGGAATCGCTGCGTGACGAGGA
GGCATTGACCCCCGATGCCGTGGTTGCACTGGCGGAGGCCGCCTACGACAAATATGGCTTCCGTGATTTCAAGCTCAAGG
GCGGCGTGCTGCGCGGCGAGCAGGAGATCGAGGCCATCCGCGCCTTGCATGCACGCTTCCCGCAGGCGCGTATCACGCTC
GACCCCAATGGCGCGTGGTCGCTGGAGCAGGCCATCGCGCTGTGCCGCGATCTGCACGGCGTGCTGGCGTATGCCGAAGA
CCCATGTGGTGCGCAGGATGGGTATTCCGGGCGCGAAGTCATGGCCGAGTTTCGGCGTGCCACCGGCCTGCCCACGGCCA
CCAACATGATCGCCACCGATTGGCGCCAGATGGGCCACGCCATCCAGCTGCAATCGGTGGATATTCCATTGGCCGATCCG
CATTTCTGGACGCTGCAAGGTTCGGTGCGCGTTGCGCAGATGTGTCGCGATTGGGGCCTGACCTGGGGTTCGCATTCCAA
CAATCACTTCGATATTTCGCTGGCCATGTTCACCCACGTGGCCGCCGCCGCACCCGGGCGCGTAACGGCGATCGATACGC
ACTGGATCTGGCAGGACGGTCAACGCCTGACGCACGCGCCGTTGACAATTGCAGGCGGCCAGATCCAGGTGCCTGCCAAA
CCGGGCCTGGGCATCGAGCTGGACATCGACGCACTGGATGCGGCGCATCGCACCTATCAGAGCCTGAGCCTGGGTGCGCG
CGACGACGCGGCAGGCATGCAGTGCCTGATTCCGAACTGGGCATTCGACAACAAGCGTCCGTGCCTGGTGCGCTAG

Upstream 100 bases:

>100_bases
GTGGTGGTCGGCCAAATCAAGCGGGTGGAGCTCAAGGCGCCGCCTGGCGGCCCATCCATGCCCCTTCCTGCAGCGCTGTG
ACTCACGCGGAGATCCTTCA

Downstream 100 bases:

>100_bases
CTGTGGCGTCGCAGGCCAACACGGGTGCATTGCGCCAGGTTGCATGCACCGCTTCACCACCGCGGAGGGCGGTTGACATG
AAACACGTACTGGCACTCGG

Product: glucarate hydratase

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNIHSSSARSSATPRITDVRVVPVAGQDSMLLNLSGAHAPYFTRNVLVLHDSSGRIGVGEVPGGEAIAALLREAAGLILD
RPIADYQRILNQVRATFADRDSAGRGLQTFDLRITIHAVTAIESALLDLLGQFLEQPVAALLGEGQQRDAVDVLGYLFFI
GDRRKASVAYRDGSGARDRWESLRDEEALTPDAVVALAEAAYDKYGFRDFKLKGGVLRGEQEIEAIRALHARFPQARITL
DPNGAWSLEQAIALCRDLHGVLAYAEDPCGAQDGYSGREVMAEFRRATGLPTATNMIATDWRQMGHAIQLQSVDIPLADP
HFWTLQGSVRVAQMCRDWGLTWGSHSNNHFDISLAMFTHVAAAAPGRVTAIDTHWIWQDGQRLTHAPLTIAGGQIQVPAK
PGLGIELDIDALDAAHRTYQSLSLGARDDAAGMQCLIPNWAFDNKRPCLVR

Sequences:

>Translated_451_residues
MNIHSSSARSSATPRITDVRVVPVAGQDSMLLNLSGAHAPYFTRNVLVLHDSSGRIGVGEVPGGEAIAALLREAAGLILD
RPIADYQRILNQVRATFADRDSAGRGLQTFDLRITIHAVTAIESALLDLLGQFLEQPVAALLGEGQQRDAVDVLGYLFFI
GDRRKASVAYRDGSGARDRWESLRDEEALTPDAVVALAEAAYDKYGFRDFKLKGGVLRGEQEIEAIRALHARFPQARITL
DPNGAWSLEQAIALCRDLHGVLAYAEDPCGAQDGYSGREVMAEFRRATGLPTATNMIATDWRQMGHAIQLQSVDIPLADP
HFWTLQGSVRVAQMCRDWGLTWGSHSNNHFDISLAMFTHVAAAAPGRVTAIDTHWIWQDGQRLTHAPLTIAGGQIQVPAK
PGLGIELDIDALDAAHRTYQSLSLGARDDAAGMQCLIPNWAFDNKRPCLVR
>Mature_451_residues
MNIHSSSARSSATPRITDVRVVPVAGQDSMLLNLSGAHAPYFTRNVLVLHDSSGRIGVGEVPGGEAIAALLREAAGLILD
RPIADYQRILNQVRATFADRDSAGRGLQTFDLRITIHAVTAIESALLDLLGQFLEQPVAALLGEGQQRDAVDVLGYLFFI
GDRRKASVAYRDGSGARDRWESLRDEEALTPDAVVALAEAAYDKYGFRDFKLKGGVLRGEQEIEAIRALHARFPQARITL
DPNGAWSLEQAIALCRDLHGVLAYAEDPCGAQDGYSGREVMAEFRRATGLPTATNMIATDWRQMGHAIQLQSVDIPLADP
HFWTLQGSVRVAQMCRDWGLTWGSHSNNHFDISLAMFTHVAAAAPGRVTAIDTHWIWQDGQRLTHAPLTIAGGQIQVPAK
PGLGIELDIDALDAAHRTYQSLSLGARDDAAGMQCLIPNWAFDNKRPCLVR

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=441, Percent_Identity=68.9342403628118, Blast_Score=649, Evalue=0.0,
Organism=Escherichia coli, GI1789151, Length=445, Percent_Identity=60.8988764044944, Blast_Score=543, Evalue=1e-155,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017653
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 49138; Mature: 49138

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIHSSSARSSATPRITDVRVVPVAGQDSMLLNLSGAHAPYFTRNVLVLHDSSGRIGVGE
CCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCCEEECEEEEEECCCCCEECCC
VPGGEAIAALLREAAGLILDRPIADYQRILNQVRATFADRDSAGRGLQTFDLRITIHAVT
CCCHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEHHH
AIESALLDLLGQFLEQPVAALLGEGQQRDAVDVLGYLFFIGDRRKASVAYRDGSGARDRW
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHH
ESLRDEEALTPDAVVALAEAAYDKYGFRDFKLKGGVLRGEQEIEAIRALHARFPQARITL
HHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCEECCHHHHHHHHHHHHCCCCEEEEE
DPNGAWSLEQAIALCRDLHGVLAYAEDPCGAQDGYSGREVMAEFRRATGLPTATNMIATD
CCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
WRQMGHAIQLQSVDIPLADPHFWTLQGSVRVAQMCRDWGLTWGSHSNNHFDISLAMFTHV
HHHCCCEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHH
AAAAPGRVTAIDTHWIWQDGQRLTHAPLTIAGGQIQVPAKPGLGIELDIDALDAAHRTYQ
HHCCCCCEEEEEEEEEECCCCEEEECCEEEECCEEEECCCCCCCEEEEHHHHHHHHHHHH
SLSLGARDDAAGMQCLIPNWAFDNKRPCLVR
HHCCCCCCCCCCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MNIHSSSARSSATPRITDVRVVPVAGQDSMLLNLSGAHAPYFTRNVLVLHDSSGRIGVGE
CCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCCEEECEEEEEECCCCCEECCC
VPGGEAIAALLREAAGLILDRPIADYQRILNQVRATFADRDSAGRGLQTFDLRITIHAVT
CCCHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEHHH
AIESALLDLLGQFLEQPVAALLGEGQQRDAVDVLGYLFFIGDRRKASVAYRDGSGARDRW
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHH
ESLRDEEALTPDAVVALAEAAYDKYGFRDFKLKGGVLRGEQEIEAIRALHARFPQARITL
HHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCEECCHHHHHHHHHHHHCCCCEEEEE
DPNGAWSLEQAIALCRDLHGVLAYAEDPCGAQDGYSGREVMAEFRRATGLPTATNMIATD
CCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
WRQMGHAIQLQSVDIPLADPHFWTLQGSVRVAQMCRDWGLTWGSHSNNHFDISLAMFTHV
HHHCCCEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHH
AAAAPGRVTAIDTHWIWQDGQRLTHAPLTIAGGQIQVPAKPGLGIELDIDALDAAHRTYQ
HHCCCCCEEEEEEEEEECCCCEEEECCEEEECCEEEECCCCCCCEEEEHHHHHHHHHHHH
SLSLGARDDAAGMQCLIPNWAFDNKRPCLVR
HHCCCCCCCCCCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9772161 [H]