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The map label for this gene is glnG [H]

Identifier: 66048046

GI number: 66048046

Start: 5718853

End: 5720289

Strand: Direct

Name: glnG [H]

Synonym: Psyr_4822

Alternate gene names: 66048046

Gene position: 5718853-5720289 (Clockwise)

Preceding gene: 66048045

Following gene: 66048052

Centisome position: 93.85

GC content: 63.26

Gene sequence:

>1437_bases
ATGAGCCGTAGTGAAACTGTCTGGATCGTAGACGACGATCGTTCTATCCGCTGGGTACTGGAAAAAGCCTTGCAACAGGA
AGGCATGACCACGCAAAGCTTCGACAGCGCCGACGGGGTGATGAGCCGTCTGGCCCGTCAGCAACCGGACGTGATCATTT
CCGACATCCGCATGCCGGGTGCCAGTGGCCTGGACCTGTTGGCGCGGATTCGCGAGCAGCATCCGCGTCTGCCGGTGATC
ATCATGACCGCCCATTCGGACCTGGACAGCGCCGTGGCGTCCTATCAGGGCGGTGCGTTCGAGTACCTGCCCAAGCCATT
CGACGTCGATGAAGCCGTGTCGCTGGTCAAGCGCGCCAATCAGCACGCTCAGGAACAACAGGGCCTGGACGTCGCCCCGA
CGCTGACCCGCACTCCCGAGATCATTGGCGAAGCACCAGCGATGCAGGAAGTGTTTCGCGCCATCGGCCGTCTGAGCCAC
TCCAACATCACGGTGTTGATCAATGGTGAGTCGGGTACTGGCAAGGAACTGGTCGCTCACGCATTGCATCGCCACAGCCC
GCGTTCGGCCTCGCCGTTCATTGCGCTGAACATGGCGGCGATCCCCAAGGACCTGATGGAGTCCGAGCTGTTCGGGCATG
AGAAAGGCGCGTTCACCGGCGCGGCCAACCTGCGCCGTGGACGCTTCGAACAGGCCGACGGCGGCACGCTGTTTCTCGAT
GAAATCGGTGACATGCCTGCCGACACCCAGACCCGTCTGTTGCGTGTCCTGGCGGATGGCGAGTTCTACCGGGTCGGCGG
TCACACGCCGGTCAAGGTCGACGTGCGCATCATTGCCGCCACCCACCAGAATCTGGAAACCCTGGTGCAGGCCGGCAAGT
TCCGCGAGGACTTGTTCCACCGCCTGAACGTGATCCGAATCCACATTCCGCGCATGTCGGACCGTCGCGAAGACATCCCG
ACCCTGGCCAAGCACTTCCTCAGCCGCGCGGCCCAGGAACTGGCGGTCGAACCCAAGCTGCTCAAGGCCGAGACCGAGGA
ATACCTCAAGCATCTGCCGTGGCCTGGCAACGTTCGTCAGCTGGAAAACACCTGCCGCTGGATCACGGTCATGGCCTCGG
GTCGTGAAGTGCACATCAGCGATCTGCCGCCGGAACTGCTGAGCCTGCCGCAGGACGCCGCGCCGGTCACCAACTGGGAA
CAGGCATTGCGCCAGTGGGCCGATCAGGCTTTGTCTCGCGGTCAGTCCAGCCTGCTGGACAGCGCCGTGCCGACCTTCGA
GCGGATCATGATCGAAACCGCCCTCAAACACACCGCCGGCCGCCGCCGCGACGCCGCCGTATTGCTGGGCTGGGGCCGCA
ACACGCTGACCCGCAAGATCAAGGAACTGGGCATGAAGATCGATGGCGGGGATGACGACGAGGCCGATGAGGGCTGA

Upstream 100 bases:

>100_bases
ACATCATCAGCCAGCACCAGGGTCTGATCGAGTGTGACAGCCATCCCGGCCACACCACATTCTCGATCTTCCTGCCGCTG
GAACAAGGAGCAGCTTCGAC

Downstream 100 bases:

>100_bases
GTGGTTATATGATGGTTGTTCATGCCTACGCGGAGCGCTCAGCGTTATACAAGTCCGCTTTTGATTCTGGCCGTAGGGCG
TAGGAGCGAACTTGTTCGCG

Product: helix-turn-helix, Fis-type:nitrogen regulation protein NR(I)

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSRSETVWIVDDDRSIRWVLEKALQQEGMTTQSFDSADGVMSRLARQQPDVIISDIRMPGASGLDLLARIREQHPRLPVI
IMTAHSDLDSAVASYQGGAFEYLPKPFDVDEAVSLVKRANQHAQEQQGLDVAPTLTRTPEIIGEAPAMQEVFRAIGRLSH
SNITVLINGESGTGKELVAHALHRHSPRSASPFIALNMAAIPKDLMESELFGHEKGAFTGAANLRRGRFEQADGGTLFLD
EIGDMPADTQTRLLRVLADGEFYRVGGHTPVKVDVRIIAATHQNLETLVQAGKFREDLFHRLNVIRIHIPRMSDRREDIP
TLAKHFLSRAAQELAVEPKLLKAETEEYLKHLPWPGNVRQLENTCRWITVMASGREVHISDLPPELLSLPQDAAPVTNWE
QALRQWADQALSRGQSSLLDSAVPTFERIMIETALKHTAGRRRDAAVLLGWGRNTLTRKIKELGMKIDGGDDDEADEG

Sequences:

>Translated_478_residues
MSRSETVWIVDDDRSIRWVLEKALQQEGMTTQSFDSADGVMSRLARQQPDVIISDIRMPGASGLDLLARIREQHPRLPVI
IMTAHSDLDSAVASYQGGAFEYLPKPFDVDEAVSLVKRANQHAQEQQGLDVAPTLTRTPEIIGEAPAMQEVFRAIGRLSH
SNITVLINGESGTGKELVAHALHRHSPRSASPFIALNMAAIPKDLMESELFGHEKGAFTGAANLRRGRFEQADGGTLFLD
EIGDMPADTQTRLLRVLADGEFYRVGGHTPVKVDVRIIAATHQNLETLVQAGKFREDLFHRLNVIRIHIPRMSDRREDIP
TLAKHFLSRAAQELAVEPKLLKAETEEYLKHLPWPGNVRQLENTCRWITVMASGREVHISDLPPELLSLPQDAAPVTNWE
QALRQWADQALSRGQSSLLDSAVPTFERIMIETALKHTAGRRRDAAVLLGWGRNTLTRKIKELGMKIDGGDDDEADEG
>Mature_477_residues
SRSETVWIVDDDRSIRWVLEKALQQEGMTTQSFDSADGVMSRLARQQPDVIISDIRMPGASGLDLLARIREQHPRLPVII
MTAHSDLDSAVASYQGGAFEYLPKPFDVDEAVSLVKRANQHAQEQQGLDVAPTLTRTPEIIGEAPAMQEVFRAIGRLSHS
NITVLINGESGTGKELVAHALHRHSPRSASPFIALNMAAIPKDLMESELFGHEKGAFTGAANLRRGRFEQADGGTLFLDE
IGDMPADTQTRLLRVLADGEFYRVGGHTPVKVDVRIIAATHQNLETLVQAGKFREDLFHRLNVIRIHIPRMSDRREDIPT
LAKHFLSRAAQELAVEPKLLKAETEEYLKHLPWPGNVRQLENTCRWITVMASGREVHISDLPPELLSLPQDAAPVTNWEQ
ALRQWADQALSRGQSSLLDSAVPTFERIMIETALKHTAGRRRDAAVLLGWGRNTLTRKIKELGMKIDGGDDDEADEG

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=464, Percent_Identity=68.5344827586207, Blast_Score=645, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=475, Percent_Identity=40.4210526315789, Blast_Score=345, Evalue=3e-96,
Organism=Escherichia coli, GI1790437, Length=454, Percent_Identity=40.3083700440529, Blast_Score=321, Evalue=7e-89,
Organism=Escherichia coli, GI1788905, Length=370, Percent_Identity=42.7027027027027, Blast_Score=284, Evalue=1e-77,
Organism=Escherichia coli, GI87082117, Length=336, Percent_Identity=42.5595238095238, Blast_Score=245, Evalue=5e-66,
Organism=Escherichia coli, GI1789233, Length=335, Percent_Identity=42.089552238806, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1789087, Length=331, Percent_Identity=42.2960725075529, Blast_Score=234, Evalue=9e-63,
Organism=Escherichia coli, GI87082152, Length=340, Percent_Identity=40, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1787583, Length=326, Percent_Identity=39.2638036809816, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1786524, Length=335, Percent_Identity=40.5970149253731, Blast_Score=215, Evalue=6e-57,
Organism=Escherichia coli, GI87081872, Length=227, Percent_Identity=45.374449339207, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI87081858, Length=408, Percent_Identity=28.4313725490196, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI1789828, Length=269, Percent_Identity=34.5724907063197, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1790860, Length=113, Percent_Identity=36.283185840708, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1789809, Length=190, Percent_Identity=25.7894736842105, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786599, Length=150, Percent_Identity=30, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1786911, Length=106, Percent_Identity=33.0188679245283, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790552, Length=124, Percent_Identity=32.258064516129, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53150; Mature: 53019

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSETVWIVDDDRSIRWVLEKALQQEGMTTQSFDSADGVMSRLARQQPDVIISDIRMPG
CCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCC
ASGLDLLARIREQHPRLPVIIMTAHSDLDSAVASYQGGAFEYLPKPFDVDEAVSLVKRAN
CCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHH
QHAQEQQGLDVAPTLTRTPEIIGEAPAMQEVFRAIGRLSHSNITVLINGESGTGKELVAH
HHHHHHCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHH
ALHRHSPRSASPFIALNMAAIPKDLMESELFGHEKGAFTGAANLRRGRFEQADGGTLFLD
HHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCEEEHH
EIGDMPADTQTRLLRVLADGEFYRVGGHTPVKVDVRIIAATHQNLETLVQAGKFREDLFH
HHCCCCCCHHHHHHHHHHCCCEEEECCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHH
RLNVIRIHIPRMSDRREDIPTLAKHFLSRAAQELAVEPKLLKAETEEYLKHLPWPGNVRQ
HCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHH
LENTCRWITVMASGREVHISDLPPELLSLPQDAAPVTNWEQALRQWADQALSRGQSSLLD
HHHHCEEEEEEECCCEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHH
SAVPTFERIMIETALKHTAGRRRDAAVLLGWGRNTLTRKIKELGMKIDGGDDDEADEG
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRSETVWIVDDDRSIRWVLEKALQQEGMTTQSFDSADGVMSRLARQQPDVIISDIRMPG
CCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCC
ASGLDLLARIREQHPRLPVIIMTAHSDLDSAVASYQGGAFEYLPKPFDVDEAVSLVKRAN
CCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHH
QHAQEQQGLDVAPTLTRTPEIIGEAPAMQEVFRAIGRLSHSNITVLINGESGTGKELVAH
HHHHHHCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHH
ALHRHSPRSASPFIALNMAAIPKDLMESELFGHEKGAFTGAANLRRGRFEQADGGTLFLD
HHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCEEEHH
EIGDMPADTQTRLLRVLADGEFYRVGGHTPVKVDVRIIAATHQNLETLVQAGKFREDLFH
HHCCCCCCHHHHHHHHHHCCCEEEECCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHH
RLNVIRIHIPRMSDRREDIPTLAKHFLSRAAQELAVEPKLLKAETEEYLKHLPWPGNVRQ
HCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHH
LENTCRWITVMASGREVHISDLPPELLSLPQDAAPVTNWEQALRQWADQALSRGQSSLLD
HHHHCEEEEEEECCCEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHH
SAVPTFERIMIETALKHTAGRRRDAAVLLGWGRNTLTRKIKELGMKIDGGDDDEADEG
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]