Click here to switch to the map view.

The map label for this gene is ntrB [H]

Identifier: 66048045

GI number: 66048045

Start: 5717771

End: 5718856

Strand: Direct

Name: ntrB [H]

Synonym: Psyr_4821

Alternate gene names: 66048045

Gene position: 5717771-5718856 (Clockwise)

Preceding gene: 66048044

Following gene: 66048046

Centisome position: 93.83

GC content: 59.48

Gene sequence:

>1086_bases
ATGACTATCAGCGATGCGCTGCACAGACTGTTACTGGATAACCTGACCACTGCCACCATTCTGCTCAACGCCGACCTGCG
ACTTGAGTACATGAACCCGGCGGCGGAAATGCTGCTGGCCATCAGCGGGCAGCGCAGCCATGGGCAATTCATCAGCGAGC
TGTTCACCGAGTCGGCCGAAGCATTGAGTTCGCTGCGTCAGGCAGTCGAACAGGCTCATCCTTTTACCAAGCGCGAAGCG
ATGCTCACTGCACTGACCGGCCAGACCCTGACGGTCGACTACGCGGTGACGCCTATCCTTAGCAAGGGCGATACCCTGCT
GCTGCTTGAAGTGCATCCGCGTGACCGGCTGTTGCGTATCACCAAGGAAGAGGCTCAGCTGTCCAAGCAGGAAACCACCA
AGATGCTGGTGCGTGGCCTGGCTCACGAGATCAAGAATCCACTGGGCGGCATTCGCGGGGCCGCACAATTGCTGGCCCGC
GAGCTGCCTGAAGAAAGCCTCAAGGACTACACCAACGTCATTATCGAAGAGGCCGACCGCCTGCGTAATCTGGTCGACCG
CATGCTCGGCTCCAACAAGCTGCCATCGCTGGCGATGACCAACGTGCACGAAGTGCTGGAGCGCGTGTGCAGCCTGGTCG
AGGCCGAAAGCCAGGGTTGCATTACCTTGGTGCGCGACTACGACCCAAGCATCCCGGACGTGTTGATCGACCGCGAGCAA
ATGATCCAGGCGGTGCTCAATATCGTGCGCAACGCCATGCAGGCCATCAGCGGCCAGAACGAGTTGCGCCTTGGCCGGAT
CACCCTGCGTACCCGCGCCATGCGCCAGTTCACCATCGGCCACGTTCGCCATCGACTGGTGAGCAAGATCGAAATCATCG
ACAACGGCCCCGGTATTCCGGCCGAACTTCAGGAAACCATTTTCTATCCGATGGTCAGTGGCCGCCCGGATGGCACGGGA
CTAGGTCTTGCCATTACCCAGAACATCATCAGCCAGCACCAGGGTCTGATCGAGTGTGACAGCCATCCCGGCCACACCAC
ATTCTCGATCTTCCTGCCGCTGGAACAAGGAGCAGCTTCGACATGA

Upstream 100 bases:

>100_bases
CTGGCAAAGCACCGTATTGGTGGCCTTGCTCCTGCTGGTGCAGACCAACCAGCACCCGCATTGCGCGACTGCAATGTGCC
AAAAGAGGTCAACACGATTC

Downstream 100 bases:

>100_bases
GCCGTAGTGAAACTGTCTGGATCGTAGACGACGATCGTTCTATCCGCTGGGTACTGGAAAAAGCCTTGCAACAGGAAGGC
ATGACCACGCAAAGCTTCGA

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MTISDALHRLLLDNLTTATILLNADLRLEYMNPAAEMLLAISGQRSHGQFISELFTESAEALSSLRQAVEQAHPFTKREA
MLTALTGQTLTVDYAVTPILSKGDTLLLLEVHPRDRLLRITKEEAQLSKQETTKMLVRGLAHEIKNPLGGIRGAAQLLAR
ELPEESLKDYTNVIIEEADRLRNLVDRMLGSNKLPSLAMTNVHEVLERVCSLVEAESQGCITLVRDYDPSIPDVLIDREQ
MIQAVLNIVRNAMQAISGQNELRLGRITLRTRAMRQFTIGHVRHRLVSKIEIIDNGPGIPAELQETIFYPMVSGRPDGTG
LGLAITQNIISQHQGLIECDSHPGHTTFSIFLPLEQGAAST

Sequences:

>Translated_361_residues
MTISDALHRLLLDNLTTATILLNADLRLEYMNPAAEMLLAISGQRSHGQFISELFTESAEALSSLRQAVEQAHPFTKREA
MLTALTGQTLTVDYAVTPILSKGDTLLLLEVHPRDRLLRITKEEAQLSKQETTKMLVRGLAHEIKNPLGGIRGAAQLLAR
ELPEESLKDYTNVIIEEADRLRNLVDRMLGSNKLPSLAMTNVHEVLERVCSLVEAESQGCITLVRDYDPSIPDVLIDREQ
MIQAVLNIVRNAMQAISGQNELRLGRITLRTRAMRQFTIGHVRHRLVSKIEIIDNGPGIPAELQETIFYPMVSGRPDGTG
LGLAITQNIISQHQGLIECDSHPGHTTFSIFLPLEQGAAST
>Mature_360_residues
TISDALHRLLLDNLTTATILLNADLRLEYMNPAAEMLLAISGQRSHGQFISELFTESAEALSSLRQAVEQAHPFTKREAM
LTALTGQTLTVDYAVTPILSKGDTLLLLEVHPRDRLLRITKEEAQLSKQETTKMLVRGLAHEIKNPLGGIRGAAQLLARE
LPEESLKDYTNVIIEEADRLRNLVDRMLGSNKLPSLAMTNVHEVLERVCSLVEAESQGCITLVRDYDPSIPDVLIDREQM
IQAVLNIVRNAMQAISGQNELRLGRITLRTRAMRQFTIGHVRHRLVSKIEIIDNGPGIPAELQETIFYPMVSGRPDGTGL
GLAITQNIISQHQGLIECDSHPGHTTFSIFLPLEQGAAST

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=349, Percent_Identity=46.7048710601719, Blast_Score=300, Evalue=9e-83,
Organism=Escherichia coli, GI1788549, Length=359, Percent_Identity=28.9693593314763, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1790436, Length=224, Percent_Identity=32.1428571428571, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1789808, Length=235, Percent_Identity=28.0851063829787, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87081816, Length=255, Percent_Identity=28.6274509803922, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI145693157, Length=269, Percent_Identity=26.7657992565056, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1789149, Length=253, Percent_Identity=25.6916996047431, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 39971; Mature: 39840

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISDALHRLLLDNLTTATILLNADLRLEYMNPAAEMLLAISGQRSHGQFISELFTESAE
CCHHHHHHHHHHHCCCCEEEEEECCEEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
ALSSLRQAVEQAHPFTKREAMLTALTGQTLTVDYAVTPILSKGDTLLLLEVHPRDRLLRI
HHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEEEECCCHHHHHH
TKEEAQLSKQETTKMLVRGLAHEIKNPLGGIRGAAQLLARELPEESLKDYTNVIIEEADR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LRNLVDRMLGSNKLPSLAMTNVHEVLERVCSLVEAESQGCITLVRDYDPSIPDVLIDREQ
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHCCHHH
MIQAVLNIVRNAMQAISGQNELRLGRITLRTRAMRQFTIGHVRHRLVSKIEIIDNGPGIP
HHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCC
AELQETIFYPMVSGRPDGTGLGLAITQNIISQHQGLIECDSHPGHTTFSIFLPLEQGAAS
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEEEEEEECCCCCC
T
C
>Mature Secondary Structure 
TISDALHRLLLDNLTTATILLNADLRLEYMNPAAEMLLAISGQRSHGQFISELFTESAE
CHHHHHHHHHHHCCCCEEEEEECCEEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
ALSSLRQAVEQAHPFTKREAMLTALTGQTLTVDYAVTPILSKGDTLLLLEVHPRDRLLRI
HHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEEEECCCHHHHHH
TKEEAQLSKQETTKMLVRGLAHEIKNPLGGIRGAAQLLARELPEESLKDYTNVIIEEADR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LRNLVDRMLGSNKLPSLAMTNVHEVLERVCSLVEAESQGCITLVRDYDPSIPDVLIDREQ
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHCCHHH
MIQAVLNIVRNAMQAISGQNELRLGRITLRTRAMRQFTIGHVRHRLVSKIEIIDNGPGIP
HHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCC
AELQETIFYPMVSGRPDGTGLGLAITQNIISQHQGLIECDSHPGHTTFSIFLPLEQGAAS
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEEEEEEECCCCCC
T
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]