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The map label for this gene is ribE [H]

Identifier: 66047688

GI number: 66047688

Start: 5324058

End: 5324720

Strand: Reverse

Name: ribE [H]

Synonym: Psyr_4461

Alternate gene names: 66047688

Gene position: 5324720-5324058 (Counterclockwise)

Preceding gene: 66047689

Following gene: 66047687

Centisome position: 87.38

GC content: 61.54

Gene sequence:

>663_bases
ATGTTTACCGGCATTATCGAATCCATTGGCAGCATCCGCGCCCTGACTCCCAAAGGCGGCGACGTCCGCGTTTACGTGGA
AACCGGCAAGCTTGACCTGACCGACGTCAAACTCGGCGACAGCATTGCCGTGAATGGCGTATGCCTGACCGCCGTCGAAT
TGCCCGGTGATGGCTTCTGGGCTGACGTCAGCCGGGAGACACTAACCGTCACCGCCTTCGTCGACCTGAAGAGCGGCAGC
CGGGTCAACCTGGAAAAGGCCCTGACCCCGACCACACGTCTGGGCGGCCATCTGGTCAGCGGCCATGTCGACGGCGTCGG
CGAAATCGTCGCCCGTGCAGATAACGCCCGTGCCATTCAGTTCACGGTGCGTGCGCCACGTGAACTGGCCAAATACATCG
CCCACAAAGGCTCGATCACGGTCGACGGCACCAGCCTGACTGTCAATTCGGTCAACGGCGCGGAGTTTGAACTGACCATC
GTGCCTCATACCTGGAGCGAAACGATCATGGCCGACTACCAGCCCGGCCGTAAGGTCAACCTGGAAGTCGATCTGCTGGC
GCGTTATCTGGAGCGCCTGCTGCTGGGTGACAAGGCCGCCGAGCCGGGCCACGGCACGATCACCGAAAGCTTCCTGGCCG
AGAACGGCTACCTCAAATCCTGA

Upstream 100 bases:

>100_bases
TCGGTAATGACTGGCGAGTCATCGCTCTCCCTGTGCGCACACCCGGCGTATAATTCCCGGCTTTCGCCTTGCGCGCAGCC
CTTGTTCTCCCGGAGGACCC

Downstream 100 bases:

>100_bases
ATTGATTGAAGGGGGTGCCGCGTGGCGCTCAACAGTATCGAAGAACTGGTCGAAGACATTCGCCAGGGCAAGATGGTCAT
CCTCATGGATGACGAAGACC

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MFTGIIESIGSIRALTPKGGDVRVYVETGKLDLTDVKLGDSIAVNGVCLTAVELPGDGFWADVSRETLTVTAFVDLKSGS
RVNLEKALTPTTRLGGHLVSGHVDGVGEIVARADNARAIQFTVRAPRELAKYIAHKGSITVDGTSLTVNSVNGAEFELTI
VPHTWSETIMADYQPGRKVNLEVDLLARYLERLLLGDKAAEPGHGTITESFLAENGYLKS

Sequences:

>Translated_220_residues
MFTGIIESIGSIRALTPKGGDVRVYVETGKLDLTDVKLGDSIAVNGVCLTAVELPGDGFWADVSRETLTVTAFVDLKSGS
RVNLEKALTPTTRLGGHLVSGHVDGVGEIVARADNARAIQFTVRAPRELAKYIAHKGSITVDGTSLTVNSVNGAEFELTI
VPHTWSETIMADYQPGRKVNLEVDLLARYLERLLLGDKAAEPGHGTITESFLAENGYLKS
>Mature_220_residues
MFTGIIESIGSIRALTPKGGDVRVYVETGKLDLTDVKLGDSIAVNGVCLTAVELPGDGFWADVSRETLTVTAFVDLKSGS
RVNLEKALTPTTRLGGHLVSGHVDGVGEIVARADNARAIQFTVRAPRELAKYIAHKGSITVDGTSLTVNSVNGAEFELTI
VPHTWSETIMADYQPGRKVNLEVDLLARYLERLLLGDKAAEPGHGTITESFLAENGYLKS

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=211, Percent_Identity=35.0710900473934, Blast_Score=114, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6319733, Length=206, Percent_Identity=39.3203883495146, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 23536; Mature: 23536

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIIESIGSIRALTPKGGDVRVYVETGKLDLTDVKLGDSIAVNGVCLTAVELPGDGFW
CCCHHHHHCCCEEEECCCCCCEEEEEECCCEEEEEEECCCCEEECCEEEEEEEECCCCEE
ADVSRETLTVTAFVDLKSGSRVNLEKALTPTTRLGGHLVSGHVDGVGEIVARADNARAIQ
EECCCCEEEEEEEEEECCCCEEEHHHHCCCCHHCCCEEEECCCCCHHHHHCCCCCCEEEE
FTVRAPRELAKYIAHKGSITVDGTSLTVNSVNGAEFELTIVPHTWSETIMADYQPGRKVN
EEECCHHHHHHHHHCCCCEEECCCEEEEECCCCCEEEEEEECCCCCCCEEECCCCCCEEE
LEVDLLARYLERLLLGDKAAEPGHGTITESFLAENGYLKS
EEHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MFTGIIESIGSIRALTPKGGDVRVYVETGKLDLTDVKLGDSIAVNGVCLTAVELPGDGFW
CCCHHHHHCCCEEEECCCCCCEEEEEECCCEEEEEEECCCCEEECCEEEEEEEECCCCEE
ADVSRETLTVTAFVDLKSGSRVNLEKALTPTTRLGGHLVSGHVDGVGEIVARADNARAIQ
EECCCCEEEEEEEEEECCCCEEEHHHHCCCCHHCCCEEEECCCCCHHHHHCCCCCCEEEE
FTVRAPRELAKYIAHKGSITVDGTSLTVNSVNGAEFELTIVPHTWSETIMADYQPGRKVN
EEECCHHHHHHHHHCCCCEEECCCEEEEECCCCCEEEEEEECCCCCCCEEECCCCCCEEE
LEVDLLARYLERLLLGDKAAEPGHGTITESFLAENGYLKS
EEHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144477 [H]