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The map label for this gene is nirA [H]

Identifier: 66047644

GI number: 66047644

Start: 5280617

End: 5281960

Strand: Reverse

Name: nirA [H]

Synonym: Psyr_4417

Alternate gene names: 66047644

Gene position: 5281960-5280617 (Counterclockwise)

Preceding gene: 66047648

Following gene: 66047643

Centisome position: 86.68

GC content: 65.92

Gene sequence:

>1344_bases
TTGAACGAGCAGCAGTCCGCTCGCACATCCTCTGCAACGCTACGCCCCTCGGCCTGTCCGGGGTTGCTGCGTATCGTCCC
GGCACTGGACGGCGGGATCTGCCGGATCAAGCTGGCGGGCGGGGTGATCAGCAGTGCGCAGGCTATGGCCGTTGCCGAGG
CCGCGCGCACGTACGCACAGGGGGTTATCGAAGCCACCAACCGCGCCAACCTGCAGATTCGCGGCATTGGTGCCGATCAT
GACGGGTTGATCGGTGCCTTGATGGCGGCAGGGCTCGGCCCCGCCAACCCGGCCAGCGACGATGTGCGTAACCTGATGCT
CAGCCCGACGGCCGGGCTGGACCCGCAGATGCTCTTCGATGCCCGGCCGCTGGCAGCGCAGATTCTCGACGCGCTGGAAC
ATCACCCGCGTTTTCATGAGCTGTCACCCAAGTTCGCCTTGTCGCTGGATGCTGGTGAAGCGTTGGTCATGCTCGAACAC
CCGCACGACGTGTGGCTGTCGGCGCTGAAACGCGACGACGAAGTGCTGCTGGCGTTCGGCCTGGCCGGCTGCCCGGCCCA
TGACCGACCGCTGGCCGCTGTCCCTCTAGCCGAAGGGAAGATGCTGGTTGTCGCACTGCTGGAGCTGTTTCTCGACCTGG
CCCGTCCCGAGCACACCCGGATGCGGCACCTGCTGACCGAGGTATCGACCGCCGATCTGCTGCGTGAGCTGTCGACCCGG
TCCGCCTGCACAGTGCGGACCGATAAGGCGATCACCGATTGGCAGCGTCCGGCCATTCAAGGCACTCGGCATATCGGTGT
TTATCCACAGGCCCAGCCGAATCAGGTGGCGGTGGGCGCTGCAGTACCGTTGGGGCGTCTCGATGCGGCGATGCTTTGCA
GCGTGGCGCAACTGGCTGCGGATCAGGGCGACGGCACGCTGCGCCTGACGCCCTGGCAAAGCCTGTTGCTGCCCAACGTG
CCGGTCGAGCGCGCGGCAGCGGTGCTGGACGAGTTGCAGGCATCCGGCTTCGCAGGTTCTGCCGAGCAACCGCTGGCGCA
GATGCTCGCCTGTACCGGTTCTGCCGGATGCGCCAAGGGGCTGGCCGACACCAAGGCCGACGCCCTGCAACTGGCTGCGG
CACTTGATACCGGCCAGGCTATTCACTTGAGCGGCTGCACGCGTTCCTGCGCCGCCGCCCACGTAGCGCCAGTGACCTTG
CTGGCTGTCGCGCCGGGCCGTTACGATCTTTATTTTCGCGATGCAGCGCATGCCGGTTTCGGCGTGCTGCGCGCGCGCAA
CCTTACTATTGAAGCAGTCGGCGCGCAGTTGAACGCCGGCTCACGGAGCAACATGCATGATTGA

Upstream 100 bases:

>100_bases
TTTTCATGCCTGCGGACAAAGCAGGCATAATACCGCGCCCGACGGGGTACAAGTGCTCTAATCAACGTCCGTTTCCAGCC
AGTTCACATCGGGTGACCCA

Downstream 100 bases:

>100_bases
TTACATCCGCGATGGTCAGGAGATATATCGCAACTCTTTCACGATCATTCGCGCCGAAGCGCGTCTTGACACGATCCCTG
CCGACCTGGAAAAACTCGCC

Product: ferredoxin--nitrite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MNEQQSARTSSATLRPSACPGLLRIVPALDGGICRIKLAGGVISSAQAMAVAEAARTYAQGVIEATNRANLQIRGIGADH
DGLIGALMAAGLGPANPASDDVRNLMLSPTAGLDPQMLFDARPLAAQILDALEHHPRFHELSPKFALSLDAGEALVMLEH
PHDVWLSALKRDDEVLLAFGLAGCPAHDRPLAAVPLAEGKMLVVALLELFLDLARPEHTRMRHLLTEVSTADLLRELSTR
SACTVRTDKAITDWQRPAIQGTRHIGVYPQAQPNQVAVGAAVPLGRLDAAMLCSVAQLAADQGDGTLRLTPWQSLLLPNV
PVERAAAVLDELQASGFAGSAEQPLAQMLACTGSAGCAKGLADTKADALQLAAALDTGQAIHLSGCTRSCAAAHVAPVTL
LAVAPGRYDLYFRDAAHAGFGVLRARNLTIEAVGAQLNAGSRSNMHD

Sequences:

>Translated_447_residues
MNEQQSARTSSATLRPSACPGLLRIVPALDGGICRIKLAGGVISSAQAMAVAEAARTYAQGVIEATNRANLQIRGIGADH
DGLIGALMAAGLGPANPASDDVRNLMLSPTAGLDPQMLFDARPLAAQILDALEHHPRFHELSPKFALSLDAGEALVMLEH
PHDVWLSALKRDDEVLLAFGLAGCPAHDRPLAAVPLAEGKMLVVALLELFLDLARPEHTRMRHLLTEVSTADLLRELSTR
SACTVRTDKAITDWQRPAIQGTRHIGVYPQAQPNQVAVGAAVPLGRLDAAMLCSVAQLAADQGDGTLRLTPWQSLLLPNV
PVERAAAVLDELQASGFAGSAEQPLAQMLACTGSAGCAKGLADTKADALQLAAALDTGQAIHLSGCTRSCAAAHVAPVTL
LAVAPGRYDLYFRDAAHAGFGVLRARNLTIEAVGAQLNAGSRSNMHD
>Mature_447_residues
MNEQQSARTSSATLRPSACPGLLRIVPALDGGICRIKLAGGVISSAQAMAVAEAARTYAQGVIEATNRANLQIRGIGADH
DGLIGALMAAGLGPANPASDDVRNLMLSPTAGLDPQMLFDARPLAAQILDALEHHPRFHELSPKFALSLDAGEALVMLEH
PHDVWLSALKRDDEVLLAFGLAGCPAHDRPLAAVPLAEGKMLVVALLELFLDLARPEHTRMRHLLTEVSTADLLRELSTR
SACTVRTDKAITDWQRPAIQGTRHIGVYPQAQPNQVAVGAAVPLGRLDAAMLCSVAQLAADQGDGTLRLTPWQSLLLPNV
PVERAAAVLDELQASGFAGSAEQPLAQMLACTGSAGCAKGLADTKADALQLAAALDTGQAIHLSGCTRSCAAAHVAPVTL
LAVAPGRYDLYFRDAAHAGFGVLRARNLTIEAVGAQLNAGSRSNMHD

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

None

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.7.7.1 [H]

Molecular weight: Translated: 46787; Mature: 46787

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEQQSARTSSATLRPSACPGLLRIVPALDGGICRIKLAGGVISSAQAMAVAEAARTYAQ
CCCHHHCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
GVIEATNRANLQIRGIGADHDGLIGALMAAGLGPANPASDDVRNLMLSPTAGLDPQMLFD
HHHHCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHC
ARPLAAQILDALEHHPRFHELSPKFALSLDAGEALVMLEHPHDVWLSALKRDDEVLLAFG
CCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEEECCHHHHHHHHCCCCCEEEEEE
LAGCPAHDRPLAAVPLAEGKMLVVALLELFLDLARPEHTRMRHLLTEVSTADLLRELSTR
CCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
SACTVRTDKAITDWQRPAIQGTRHIGVYPQAQPNQVAVGAAVPLGRLDAAMLCSVAQLAA
CCEEEECCCCHHHHCCHHHCCCCEECCCCCCCCCCEEEECCCCHHHCCHHHHHHHHHHHH
DQGDGTLRLTPWQSLLLPNVPVERAAAVLDELQASGFAGSAEQPLAQMLACTGSAGCAKG
CCCCCEEEECCHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHCC
LADTKADALQLAAALDTGQAIHLSGCTRSCAAAHVAPVTLLAVAPGRYDLYFRDAAHAGF
CCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHEEEEEECCCCEEEEEECCCCCCH
GVLRARNLTIEAVGAQLNAGSRSNMHD
HHHEECCEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MNEQQSARTSSATLRPSACPGLLRIVPALDGGICRIKLAGGVISSAQAMAVAEAARTYAQ
CCCHHHCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
GVIEATNRANLQIRGIGADHDGLIGALMAAGLGPANPASDDVRNLMLSPTAGLDPQMLFD
HHHHCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHC
ARPLAAQILDALEHHPRFHELSPKFALSLDAGEALVMLEHPHDVWLSALKRDDEVLLAFG
CCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEEECCHHHHHHHHCCCCCEEEEEE
LAGCPAHDRPLAAVPLAEGKMLVVALLELFLDLARPEHTRMRHLLTEVSTADLLRELSTR
CCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
SACTVRTDKAITDWQRPAIQGTRHIGVYPQAQPNQVAVGAAVPLGRLDAAMLCSVAQLAA
CCEEEECCCCHHHHCCHHHCCCCEECCCCCCCCCCEEEECCCCHHHCCHHHHHHHHHHHH
DQGDGTLRLTPWQSLLLPNVPVERAAAVLDELQASGFAGSAEQPLAQMLACTGSAGCAKG
CCCCCEEEECCHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHCC
LADTKADALQLAAALDTGQAIHLSGCTRSCAAAHVAPVTLLAVAPGRYDLYFRDAAHAGF
CCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHEEEEEECCCCEEEEEECCCCCCH
GVLRARNLTIEAVGAQLNAGSRSNMHD
HHHEECCEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8490140 [H]