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The map label for this gene is 66047535

Identifier: 66047535

GI number: 66047535

Start: 5133734

End: 5135290

Strand: Reverse

Name: 66047535

Synonym: Psyr_4308

Alternate gene names: NA

Gene position: 5135290-5133734 (Counterclockwise)

Preceding gene: 66047536

Following gene: 66047534

Centisome position: 84.27

GC content: 59.54

Gene sequence:

>1557_bases
GTGAGCCAGTCCCTGATTGCTGCCCTGCAAAACCCGGCCCTGTTCCCCCATCCGGTGGATCAGTTCCACGTCATCGAGAC
CCATATTTCCTGGGTCCTGCTTACGGGTAACTACGCTTATAAAATGAAGAAGCCGGTGAATTTCGGTTTCCTCGATTTCA
CCACCCTGGAAGCCCGCAGCCACTTTTGCAATGAAGAGCTGCGCCTCAACCAGCGCCTGACCGATAATTTGTATCTTGAA
GTGTTGCCAATCACCGGCAGCGCAGAAGCACCGCAATTGGGCGGCGACGGCCCGGTAGTCGACTACGTGCTGAAAATGCG
CCAGTTCCCACAAAGCCAGCTGCTCAGCACCTTGCAAGCCAACGGCGAACTGACCAGCGCGCACATCGATGAACTGGCCA
GGCAGATCGCGCACTTTCACCTGAATGCCCCGAAAGTGCGGGCCACACACACCGCCGGCACGCCACACGACGTCATGGTG
CCGGTGCTGCAGAACTTCGAGCAGATCCGCCCTTTCCTCAGCGACAAGGCTGACCTGGCACAACTGGGAGCCTTGCAGGC
CTGGGCCGAAAGCAGCTTCGAGCGCCTCAAGCCACTGTTTGAACAACGCAAGCTCAATGGTTTCATCCGCGAATGCCACG
GTGATATTCACCTGGGCAACGCCACTATGATTGATGACCATGTGGTGATCTTTGACTGCATCGAGTTCAACGAGCCATTC
CGCTTCACCGACGTGTATGCCGACACCGGTTTCCTGGCGATGGACCTGGAAGACCGTGGCCTCAAAAGCCTGGCGCGGCG
CTTTATCAGCCAGTACCTGGAACTTACCGGCGACTACCAAGGCCTGGAGCTGCTGAACTTCTATAAAGCCTACCGCGCCC
TGGTACGCGCCAAGGTTGCACTGTTCAGCATGCCTTCCGAGGCAGACGCGGTGCAGCGCGCAACCACCCTGCGCCAATAC
CGCAACTACGCCAACCTGGCAGAAAGCTACAGCACCACCCCCTCGCGTTTCCTGGCCATCACTCACGGCGTATCAGCCGT
CGGCAAAAGCCACGTGGCCATGCGCCTGGTAGAAGCACTGGGGGCAATTCGCCTACGGTCGGATGTAGAGCGCAAGCGCC
TGTTCGGCGAGCAGCATGTCGAGAACAAGCCGCAGGCAGGGATCTACGCCGCAGATGCCAGCGCCGCCACCTACGCGCGC
TTGAACGAGATCGCCGACACCTTACTGCGCGCTGGTTTCCCGGTGGTACTGGATGCGACCTTCCTCAAGCGTGAACAACG
CGACGCTGCGGCGAAAATCGCTGAGGCCACCGGCGCACCGTTCCTGATCCTCGACTGCAATGCGCCACAGGCCGTGATCG
CCGCTTGGCTGGCACAACGTCAGACAGACAACAACGATCTGTCCGACGCCACCCTGACCGTCATCGAAGGGCAGCAGGCC
AATCGTGACCCGCTCAGCGCCGAAGAGTTGCTGCTCAGCAAACGCGTGGAGACCAATGAAAGCGGGACGCTTGACGCGTT
GGTTGCGCAAATTCGTCAGCGACTGTCCCGTTCGTAA

Upstream 100 bases:

>100_bases
TACTATTGATGACCAATATGAGCTGGACAGCGGTTTGCACAACCGTCTGTAGCAGCAATCATACCCTCCATGAATTTCCC
CAAACTCCGAAGGATCACAC

Downstream 100 bases:

>100_bases
GTAAAATTTATTGACATCAAGCCCTCATCAGATAAGGGTCAAGAAATACTGGCATTTTTCTTGACCCTTTTTGCGCCTTT
TTCATGCTGACGTTGCCGAC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MSQSLIAALQNPALFPHPVDQFHVIETHISWVLLTGNYAYKMKKPVNFGFLDFTTLEARSHFCNEELRLNQRLTDNLYLE
VLPITGSAEAPQLGGDGPVVDYVLKMRQFPQSQLLSTLQANGELTSAHIDELARQIAHFHLNAPKVRATHTAGTPHDVMV
PVLQNFEQIRPFLSDKADLAQLGALQAWAESSFERLKPLFEQRKLNGFIRECHGDIHLGNATMIDDHVVIFDCIEFNEPF
RFTDVYADTGFLAMDLEDRGLKSLARRFISQYLELTGDYQGLELLNFYKAYRALVRAKVALFSMPSEADAVQRATTLRQY
RNYANLAESYSTTPSRFLAITHGVSAVGKSHVAMRLVEALGAIRLRSDVERKRLFGEQHVENKPQAGIYAADASAATYAR
LNEIADTLLRAGFPVVLDATFLKREQRDAAAKIAEATGAPFLILDCNAPQAVIAAWLAQRQTDNNDLSDATLTVIEGQQA
NRDPLSAEELLLSKRVETNESGTLDALVAQIRQRLSRS

Sequences:

>Translated_518_residues
MSQSLIAALQNPALFPHPVDQFHVIETHISWVLLTGNYAYKMKKPVNFGFLDFTTLEARSHFCNEELRLNQRLTDNLYLE
VLPITGSAEAPQLGGDGPVVDYVLKMRQFPQSQLLSTLQANGELTSAHIDELARQIAHFHLNAPKVRATHTAGTPHDVMV
PVLQNFEQIRPFLSDKADLAQLGALQAWAESSFERLKPLFEQRKLNGFIRECHGDIHLGNATMIDDHVVIFDCIEFNEPF
RFTDVYADTGFLAMDLEDRGLKSLARRFISQYLELTGDYQGLELLNFYKAYRALVRAKVALFSMPSEADAVQRATTLRQY
RNYANLAESYSTTPSRFLAITHGVSAVGKSHVAMRLVEALGAIRLRSDVERKRLFGEQHVENKPQAGIYAADASAATYAR
LNEIADTLLRAGFPVVLDATFLKREQRDAAAKIAEATGAPFLILDCNAPQAVIAAWLAQRQTDNNDLSDATLTVIEGQQA
NRDPLSAEELLLSKRVETNESGTLDALVAQIRQRLSRS
>Mature_517_residues
SQSLIAALQNPALFPHPVDQFHVIETHISWVLLTGNYAYKMKKPVNFGFLDFTTLEARSHFCNEELRLNQRLTDNLYLEV
LPITGSAEAPQLGGDGPVVDYVLKMRQFPQSQLLSTLQANGELTSAHIDELARQIAHFHLNAPKVRATHTAGTPHDVMVP
VLQNFEQIRPFLSDKADLAQLGALQAWAESSFERLKPLFEQRKLNGFIRECHGDIHLGNATMIDDHVVIFDCIEFNEPFR
FTDVYADTGFLAMDLEDRGLKSLARRFISQYLELTGDYQGLELLNFYKAYRALVRAKVALFSMPSEADAVQRATTLRQYR
NYANLAESYSTTPSRFLAITHGVSAVGKSHVAMRLVEALGAIRLRSDVERKRLFGEQHVENKPQAGIYAADASAATYARL
NEIADTLLRAGFPVVLDATFLKREQRDAAAKIAEATGAPFLILDCNAPQAVIAAWLAQRQTDNNDLSDATLTVIEGQQAN
RDPLSAEELLLSKRVETNESGTLDALVAQIRQRLSRS

Specific function: Some isolated peptides of this protein are able to bind to human U937 monocytoblastic and A549 epithelial cell lines [H]

COG id: COG2187

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011009
- InterPro:   IPR010488 [H]

Pfam domain/function: PF06414 Zeta_toxin [H]

EC number: NA

Molecular weight: Translated: 57796; Mature: 57665

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSLIAALQNPALFPHPVDQFHVIETHISWVLLTGNYAYKMKKPVNFGFLDFTTLEARS
CCCHHHHHHCCCCCCCCCCCHHEEEEEEEEEEEEECCEEEEECCCCCCCCEEEEHHHHHH
HFCNEELRLNQRLTDNLYLEVLPITGSAEAPQLGGDGPVVDYVLKMRQFPQSQLLSTLQA
HHCCHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHC
NGELTSAHIDELARQIAHFHLNAPKVRATHTAGTPHDVMVPVLQNFEQIRPFLSDKADLA
CCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCHHHHHHHHCCCHHHH
QLGALQAWAESSFERLKPLFEQRKLNGFIRECHGDIHLGNATMIDDHVVIFDCIEFNEPF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEECCEEEEEEECCCCCC
RFTDVYADTGFLAMDLEDRGLKSLARRFISQYLELTGDYQGLELLNFYKAYRALVRAKVA
EEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LFSMPSEADAVQRATTLRQYRNYANLAESYSTTPSRFLAITHGVSAVGKSHVAMRLVEAL
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEHHHCHHHHHHHHHHHHHHHHH
GAIRLRSDVERKRLFGEQHVENKPQAGIYAADASAATYARLNEIADTLLRAGFPVVLDAT
HHHHHHHHHHHHHHHCHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEHH
FLKREQRDAAAKIAEATGAPFLILDCNAPQAVIAAWLAQRQTDNNDLSDATLTVIEGQQA
HHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
NRDPLSAEELLLSKRVETNESGTLDALVAQIRQRLSRS
CCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQSLIAALQNPALFPHPVDQFHVIETHISWVLLTGNYAYKMKKPVNFGFLDFTTLEARS
CCHHHHHHCCCCCCCCCCCHHEEEEEEEEEEEEECCEEEEECCCCCCCCEEEEHHHHHH
HFCNEELRLNQRLTDNLYLEVLPITGSAEAPQLGGDGPVVDYVLKMRQFPQSQLLSTLQA
HHCCHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHC
NGELTSAHIDELARQIAHFHLNAPKVRATHTAGTPHDVMVPVLQNFEQIRPFLSDKADLA
CCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCHHHHHHHHCCCHHHH
QLGALQAWAESSFERLKPLFEQRKLNGFIRECHGDIHLGNATMIDDHVVIFDCIEFNEPF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEECCEEEEEEECCCCCC
RFTDVYADTGFLAMDLEDRGLKSLARRFISQYLELTGDYQGLELLNFYKAYRALVRAKVA
EEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LFSMPSEADAVQRATTLRQYRNYANLAESYSTTPSRFLAITHGVSAVGKSHVAMRLVEAL
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEHHHCHHHHHHHHHHHHHHHHH
GAIRLRSDVERKRLFGEQHVENKPQAGIYAADASAATYARLNEIADTLLRAGFPVVLDAT
HHHHHHHHHHHHHHHCHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEHH
FLKREQRDAAAKIAEATGAPFLILDCNAPQAVIAAWLAQRQTDNNDLSDATLTVIEGQQA
HHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
NRDPLSAEELLLSKRVETNESGTLDALVAQIRQRLSRS
CCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]