Click here to switch to the map view.

The map label for this gene is gatB [H]

Identifier: 66047393

GI number: 66047393

Start: 4955603

End: 4957048

Strand: Direct

Name: gatB [H]

Synonym: Psyr_4166

Alternate gene names: 66047393

Gene position: 4955603-4957048 (Clockwise)

Preceding gene: 66047392

Following gene: 66047394

Centisome position: 81.32

GC content: 60.17

Gene sequence:

>1446_bases
ATGCAATGGGAAGTCGTCATCGGGCTGGAGATTCATACCCAGCTTTCGACCCAATCAAAGATTTTTTCCGGTAGCGCGAC
CACGTTCGGCTCCGAGCCCAACACGCAGGCCAGCCTCGTCGACCTGGGCATGCCCGGCGTATTGCCGGTGCTGAACAAGG
AAGCCGTGCGCATGGCCGTCAAGTTCGGCCTGGCCGTCAACGCCGAAATCGGCCAGCACAACGTGTTTGCCCGCAAGAAC
TACTTCTACCCGGACCTGCCCAAGGGCTATCAGATCAGCCAGATGGAGCTGCCGATTGTCGGCAAGGGCCACCTGGACAT
CCCCCTTGAAGACGGCACCGTCAAGCGCATCGGCATCACCCGTGCGCACCTGGAAGAGGACGCGGGCAAGAGCCTGCACG
AAGACTTCCAGGGCATGACCGGTATCGACCTGAACCGTGCCGGCACCCCGTTGCTGGAGATCGTTTCCGAGCCGGACATG
CGCAACGCCAAGGAAGCCGTGGCTTACGTCAAGGCCATCCATGCGATTGTCCGCTACCTGGGCATCTGTGACGGCAACAT
GGCCGAAGGTTCGCTGCGCTGCGACTGCAACGTATCGATTCGCCCCAAGGGTCAGGTCGAGTTCGGCACGCGTTGCGAGA
TCAAGAACGTCAACTCGTTCCGCTTCATCGAAAAAGCGATCAACAGCGAGATTCAGCGCCAGATCGACCTGATCGAAGAC
GGTGGCAAGGTTATCCAGCAGACACGCCTGTATGACCCGAACACCAACGAAACCCGCGCCATGCGCAGCAAGGAGGAAGC
CAACGACTACCGTTACTTCCCCGACCCGGATCTGTTGCCGGTGATCATCGAGGACTCGTTCCTCGAAGAAACCCGCGCCA
CCTTGCCGGAGCTGCCACCGCAGAAACGCGAACGCTTCCAGAGCCAGTTCGGTCTGTCGACCTACGACGCCAGCGTACTG
GCTTCTAGCCGCGAGCAGGCTGATTACTTCGAACAGGTGGTGAGCATCAGTGGTGACGCCAAACTGGCAGCCAACTGGGT
CATGGTCGAGCTGGGCAGCCTGCTCAACAAGCAGGGCCTGGAAATCGACCAGTCGCCGGTCAGTGCCGAGCAGTTGGGCG
GCATGCTCAAGCGCATCACCGACAACACCATTTCCGGCAAGATCGCGAAAATGGTCTTCGAGGCGATGGCCAATGGTGAA
GGCAGCGCCGATGAAGTCATCGAAAAGCGCGGCCTGAAGCAGGTCACCGACAGCGGCGCCATCGAATCGATGCTCGACGA
GGTACTGGCAGCCAACGCGGAACAGGTCGAACAGTATCGCGCCGCCGATGAAGCCAAGCGCGGCAAGATGTTCGGCTTCT
TCGTCGGTCAGGCGATGAAAGCCTCCAAAGGCAAGGCCAACCCGCAGCAGGTCAACGAACTGCTGAAGGCCAAGCTGGAG
GGCTGA

Upstream 100 bases:

>100_bases
CCGTATTTCCAGGAAGGCCGCCTCTTGAATGTGGCGCATCAGTATCAGCAAGTCACCGACTGGCACACTCGCGCACCTGA
AGGCTTCTGAGGAGAAACAC

Downstream 100 bases:

>100_bases
TCCTCGTCACTCTCGTTCCCACGCTATGCGATCAGCGTTATACACGAGTCTTGTGGGAGCGAGCTTGCTCGCGAATAGGC
CGGTACAGCCTCTAGAGACA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MQWEVVIGLEIHTQLSTQSKIFSGSATTFGSEPNTQASLVDLGMPGVLPVLNKEAVRMAVKFGLAVNAEIGQHNVFARKN
YFYPDLPKGYQISQMELPIVGKGHLDIPLEDGTVKRIGITRAHLEEDAGKSLHEDFQGMTGIDLNRAGTPLLEIVSEPDM
RNAKEAVAYVKAIHAIVRYLGICDGNMAEGSLRCDCNVSIRPKGQVEFGTRCEIKNVNSFRFIEKAINSEIQRQIDLIED
GGKVIQQTRLYDPNTNETRAMRSKEEANDYRYFPDPDLLPVIIEDSFLEETRATLPELPPQKRERFQSQFGLSTYDASVL
ASSREQADYFEQVVSISGDAKLAANWVMVELGSLLNKQGLEIDQSPVSAEQLGGMLKRITDNTISGKIAKMVFEAMANGE
GSADEVIEKRGLKQVTDSGAIESMLDEVLAANAEQVEQYRAADEAKRGKMFGFFVGQAMKASKGKANPQQVNELLKAKLE
G

Sequences:

>Translated_481_residues
MQWEVVIGLEIHTQLSTQSKIFSGSATTFGSEPNTQASLVDLGMPGVLPVLNKEAVRMAVKFGLAVNAEIGQHNVFARKN
YFYPDLPKGYQISQMELPIVGKGHLDIPLEDGTVKRIGITRAHLEEDAGKSLHEDFQGMTGIDLNRAGTPLLEIVSEPDM
RNAKEAVAYVKAIHAIVRYLGICDGNMAEGSLRCDCNVSIRPKGQVEFGTRCEIKNVNSFRFIEKAINSEIQRQIDLIED
GGKVIQQTRLYDPNTNETRAMRSKEEANDYRYFPDPDLLPVIIEDSFLEETRATLPELPPQKRERFQSQFGLSTYDASVL
ASSREQADYFEQVVSISGDAKLAANWVMVELGSLLNKQGLEIDQSPVSAEQLGGMLKRITDNTISGKIAKMVFEAMANGE
GSADEVIEKRGLKQVTDSGAIESMLDEVLAANAEQVEQYRAADEAKRGKMFGFFVGQAMKASKGKANPQQVNELLKAKLE
G
>Mature_481_residues
MQWEVVIGLEIHTQLSTQSKIFSGSATTFGSEPNTQASLVDLGMPGVLPVLNKEAVRMAVKFGLAVNAEIGQHNVFARKN
YFYPDLPKGYQISQMELPIVGKGHLDIPLEDGTVKRIGITRAHLEEDAGKSLHEDFQGMTGIDLNRAGTPLLEIVSEPDM
RNAKEAVAYVKAIHAIVRYLGICDGNMAEGSLRCDCNVSIRPKGQVEFGTRCEIKNVNSFRFIEKAINSEIQRQIDLIED
GGKVIQQTRLYDPNTNETRAMRSKEEANDYRYFPDPDLLPVIIEDSFLEETRATLPELPPQKRERFQSQFGLSTYDASVL
ASSREQADYFEQVVSISGDAKLAANWVMVELGSLLNKQGLEIDQSPVSAEQLGGMLKRITDNTISGKIAKMVFEAMANGE
GSADEVIEKRGLKQVTDSGAIESMLDEVLAANAEQVEQYRAADEAKRGKMFGFFVGQAMKASKGKANPQQVNELLKAKLE
G

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=499, Percent_Identity=37.4749498997996, Blast_Score=338, Evalue=5e-93,
Organism=Caenorhabditis elegans, GI115534094, Length=384, Percent_Identity=34.8958333333333, Blast_Score=211, Evalue=8e-55,
Organism=Saccharomyces cerevisiae, GI6319391, Length=513, Percent_Identity=32.3586744639376, Blast_Score=235, Evalue=9e-63,
Organism=Drosophila melanogaster, GI45550808, Length=498, Percent_Identity=34.136546184739, Blast_Score=296, Evalue=2e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53041; Mature: 53041

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQWEVVIGLEIHTQLSTQSKIFSGSATTFGSEPNTQASLVDLGMPGVLPVLNKEAVRMAV
CCEEEEEEEEEEECCCHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHH
KFGLAVNAEIGQHNVFARKNYFYPDLPKGYQISQMELPIVGKGHLDIPLEDGTVKRIGIT
HHCEEEECCCCCCCEEEECCCCCCCCCCCCEECEEECCEECCCCEEEECCCCCEEEECCH
RAHLEEDAGKSLHEDFQGMTGIDLNRAGTPLLEIVSEPDMRNAKEAVAYVKAIHAIVRYL
HHHHHHHHCHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
GICDGNMAEGSLRCDCNVSIRPKGQVEFGTRCEIKNVNSFRFIEKAINSEIQRQIDLIED
HCCCCCCCCCCEEEEECEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHC
GGKVIQQTRLYDPNTNETRAMRSKEEANDYRYFPDPDLLPVIIEDSFLEETRATLPELPP
CHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHCCCCCH
QKRERFQSQFGLSTYDASVLASSREQADYFEQVVSISGDAKLAANWVMVELGSLLNKQGL
HHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
EIDQSPVSAEQLGGMLKRITDNTISGKIAKMVFEAMANGEGSADEVIEKRGLKQVTDSGA
CCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHCCHH
IESMLDEVLAANAEQVEQYRAADEAKRGKMFGFFVGQAMKASKGKANPQQVNELLKAKLE
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCEEEHHHHHHHHHCCCCCCHHHHHHHHHHHCC
G
C
>Mature Secondary Structure
MQWEVVIGLEIHTQLSTQSKIFSGSATTFGSEPNTQASLVDLGMPGVLPVLNKEAVRMAV
CCEEEEEEEEEEECCCHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHH
KFGLAVNAEIGQHNVFARKNYFYPDLPKGYQISQMELPIVGKGHLDIPLEDGTVKRIGIT
HHCEEEECCCCCCCEEEECCCCCCCCCCCCEECEEECCEECCCCEEEECCCCCEEEECCH
RAHLEEDAGKSLHEDFQGMTGIDLNRAGTPLLEIVSEPDMRNAKEAVAYVKAIHAIVRYL
HHHHHHHHCHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
GICDGNMAEGSLRCDCNVSIRPKGQVEFGTRCEIKNVNSFRFIEKAINSEIQRQIDLIED
HCCCCCCCCCCEEEEECEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHC
GGKVIQQTRLYDPNTNETRAMRSKEEANDYRYFPDPDLLPVIIEDSFLEETRATLPELPP
CHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHCCCCCH
QKRERFQSQFGLSTYDASVLASSREQADYFEQVVSISGDAKLAANWVMVELGSLLNKQGL
HHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
EIDQSPVSAEQLGGMLKRITDNTISGKIAKMVFEAMANGEGSADEVIEKRGLKQVTDSGA
CCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHCCHH
IESMLDEVLAANAEQVEQYRAADEAKRGKMFGFFVGQAMKASKGKANPQQVNELLKAKLE
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCEEEHHHHHHHHHCCCCCCHHHHHHHHHHHCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA