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The map label for this gene is nemA [H]

Identifier: 66047280

GI number: 66047280

Start: 4835628

End: 4836677

Strand: Direct

Name: nemA [H]

Synonym: Psyr_4053

Alternate gene names: 66047280

Gene position: 4835628-4836677 (Clockwise)

Preceding gene: 66047279

Following gene: 66047285

Centisome position: 79.35

GC content: 62.48

Gene sequence:

>1050_bases
ATGACGACTATCTTTGACCCGATCACCCTGGGCGATCTGCAACTGCCCAACCGCATCATCATGGCCCCACTGACGCGCTG
CCGTGCCGATGAAGGCCGTGTGCCGAACGCGATGATGGCCGAGTATTACGTGCAGCGCGCCTCGGCCGGCCTGATCCTGA
CTGAAGCCACCTCTGTGACGCCGATGGGCGTGGGCTACCCCGACACCCCGGGCATCTGGTCCAACGACCAGGTACGTGGC
TGGAGCAACATCACCAAGGCCGTGCACAACGCCGGTGGCCGTATCGCCCTGCAGTTGTGGCACGTGGGCCGTATTTCCCA
CCCGTCCTACCTGAACGGCGAAACCCCGGTCGCGCCGAGTGCCATTGCGGCTGAAGGGCACGTGAGCCTGATGCGCCCCA
TCACACCACTGCCAGTACCTCGCGCGCTGGAGCTGGCGGAAATCGGTGACATCGTCGAGGCCTATCGCGTCGGTGCGGAA
AACGCCAAGGCTGCGGGCTTCGACGGCGTGGAAGTGCATGGCGCCAACGGCTATCTGCTCGAACAGTTCCTGCTGACCGG
CACCAACCAGCGCACCGACGAGTACGGCGGTTCGGTGGAAAATCGTGCGCGTCTGCTGCTCGAAGTGACCGACGCAGTCA
TCGATGTCTGGGGCGCTGGCCGTGTAGGTGTTCACCTCTCGCCTCGCTTCGACATGCACGACATGAGCGATGCAAACCGT
GCTGAAACCTTCAGCTACGTGGCCAAAGAGCTCGGCAAGCGCGGTATCGCCTTCATCTGTGCCCGTGAGCATGATGCTGA
AGACAGCCTCGGCCCACAGTTGAAGAAGGATTTCGGCGGCGTTTATATCGCCAACGAGAAGTTCACCAAAGACAGCGCCA
ATGCCTGGCTGGCAGAAGGCAAGGCCGACGCAATCGCGTTCGGTGTGCCGTACATTGCCAACCCTGACCTGCCGGAGCGG
CTGGCCAGCGATGCCCCTCTGAACGAAGCCCATCCGGAGACGTTCTACGCCAAAGGGCCGGTCGGTTACATCGACTACCC
GCGCCTGTAA

Upstream 100 bases:

>100_bases
GGCAGTGCTGGCGCTGCTGATCACCCTGATTACTTTCCGTCAGACCGGCAATCCGGATCTGGCACACGCTACGCATTGAT
CAATTACGAGGATTCGATAC

Downstream 100 bases:

>100_bases
AACGTTACTGCAAGGCAAAAAACCCTGACTCGCAAGAGTCAGGGTTTTTTTATCGCCCGTGTAAGGGTAACCGCTACGGC
GCAACGTCAGGCTTGACTGT

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MTTIFDPITLGDLQLPNRIIMAPLTRCRADEGRVPNAMMAEYYVQRASAGLILTEATSVTPMGVGYPDTPGIWSNDQVRG
WSNITKAVHNAGGRIALQLWHVGRISHPSYLNGETPVAPSAIAAEGHVSLMRPITPLPVPRALELAEIGDIVEAYRVGAE
NAKAAGFDGVEVHGANGYLLEQFLLTGTNQRTDEYGGSVENRARLLLEVTDAVIDVWGAGRVGVHLSPRFDMHDMSDANR
AETFSYVAKELGKRGIAFICAREHDAEDSLGPQLKKDFGGVYIANEKFTKDSANAWLAEGKADAIAFGVPYIANPDLPER
LASDAPLNEAHPETFYAKGPVGYIDYPRL

Sequences:

>Translated_349_residues
MTTIFDPITLGDLQLPNRIIMAPLTRCRADEGRVPNAMMAEYYVQRASAGLILTEATSVTPMGVGYPDTPGIWSNDQVRG
WSNITKAVHNAGGRIALQLWHVGRISHPSYLNGETPVAPSAIAAEGHVSLMRPITPLPVPRALELAEIGDIVEAYRVGAE
NAKAAGFDGVEVHGANGYLLEQFLLTGTNQRTDEYGGSVENRARLLLEVTDAVIDVWGAGRVGVHLSPRFDMHDMSDANR
AETFSYVAKELGKRGIAFICAREHDAEDSLGPQLKKDFGGVYIANEKFTKDSANAWLAEGKADAIAFGVPYIANPDLPER
LASDAPLNEAHPETFYAKGPVGYIDYPRL
>Mature_348_residues
TTIFDPITLGDLQLPNRIIMAPLTRCRADEGRVPNAMMAEYYVQRASAGLILTEATSVTPMGVGYPDTPGIWSNDQVRGW
SNITKAVHNAGGRIALQLWHVGRISHPSYLNGETPVAPSAIAAEGHVSLMRPITPLPVPRALELAEIGDIVEAYRVGAEN
AKAAGFDGVEVHGANGYLLEQFLLTGTNQRTDEYGGSVENRARLLLEVTDAVIDVWGAGRVGVHLSPRFDMHDMSDANRA
ETFSYVAKELGKRGIAFICAREHDAEDSLGPQLKKDFGGVYIANEKFTKDSANAWLAEGKADAIAFGVPYIANPDLPERL
ASDAPLNEAHPETFYAKGPVGYIDYPRL

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=363, Percent_Identity=46.5564738292011, Blast_Score=294, Evalue=5e-81,
Organism=Escherichia coli, GI1789463, Length=237, Percent_Identity=35.0210970464135, Blast_Score=117, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17565138, Length=306, Percent_Identity=29.4117647058824, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17559804, Length=129, Percent_Identity=41.8604651162791, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI72001454, Length=261, Percent_Identity=29.5019157088123, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17559802, Length=263, Percent_Identity=30.4182509505703, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17564188, Length=345, Percent_Identity=26.0869565217391, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17540738, Length=308, Percent_Identity=26.6233766233766, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17566914, Length=188, Percent_Identity=30.8510638297872, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6321973, Length=381, Percent_Identity=37.2703412073491, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6325086, Length=381, Percent_Identity=36.4829396325459, Blast_Score=197, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 37812; Mature: 37681

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIFDPITLGDLQLPNRIIMAPLTRCRADEGRVPNAMMAEYYVQRASAGLILTEATSVT
CCCEECCEEECCCCCCCHHEEHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCC
PMGVGYPDTPGIWSNDQVRGWSNITKAVHNAGGRIALQLWHVGRISHPSYLNGETPVAPS
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCC
AIAAEGHVSLMRPITPLPVPRALELAEIGDIVEAYRVGAENAKAAGFDGVEVHGANGYLL
CEECCCCHHEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHH
EQFLLTGTNQRTDEYGGSVENRARLLLEVTDAVIDVWGAGRVGVHLSPRFDMHDMSDANR
HHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHH
AETFSYVAKELGKRGIAFICAREHDAEDSLGPQLKKDFGGVYIANEKFTKDSANAWLAEG
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHCCCEEEECCCCCCCCCCCEEECC
KADAIAFGVPYIANPDLPERLASDAPLNEAHPETFYAKGPVGYIDYPRL
CCCEEEECCCCCCCCCHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCC
>Mature Secondary Structure 
TTIFDPITLGDLQLPNRIIMAPLTRCRADEGRVPNAMMAEYYVQRASAGLILTEATSVT
CCEECCEEECCCCCCCHHEEHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCC
PMGVGYPDTPGIWSNDQVRGWSNITKAVHNAGGRIALQLWHVGRISHPSYLNGETPVAPS
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCC
AIAAEGHVSLMRPITPLPVPRALELAEIGDIVEAYRVGAENAKAAGFDGVEVHGANGYLL
CEECCCCHHEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHH
EQFLLTGTNQRTDEYGGSVENRARLLLEVTDAVIDVWGAGRVGVHLSPRFDMHDMSDANR
HHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHH
AETFSYVAKELGKRGIAFICAREHDAEDSLGPQLKKDFGGVYIANEKFTKDSANAWLAEG
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHCCCEEEECCCCCCCCCCCEEECC
KADAIAFGVPYIANPDLPERLASDAPLNEAHPETFYAKGPVGYIDYPRL
CCCEEEECCCCCCCCCHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]