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The map label for this gene is ygfZ [C]

Identifier: 66047189

GI number: 66047189

Start: 4709531

End: 4710478

Strand: Direct

Name: ygfZ [C]

Synonym: Psyr_3962

Alternate gene names: 66047189

Gene position: 4709531-4710478 (Clockwise)

Preceding gene: 66047186

Following gene: 66047190

Centisome position: 77.29

GC content: 59.7

Gene sequence:

>948_bases
ATGACCATGGCCCACACAGCTTTTTTCTGCACGCTATCCCATGAGGGCGTTCTCGCCGTTCGCGGCGTGGATGCCAGCAA
GTTTCTTCAGGGTCAGCTGACGTGCAACCTCAACTATCTGAATGAAGACACATCCAGCCTGGGTGCCCGTTGCACACAGA
AAGGCCGGATGCAGTCGAGTTTTCGTATCGTCTTCGAGGGCGACGGCTGCCTGCTGGCGATGGCTGGCGAACTGATCGAG
GCGCAACTGCTCGACCTGCGCAAATACGCGGTGTTCTCCAAATCGAAGCTCACGGACGAAAGCGCAGACTGGGTGCGCTT
CGGTCTGCAGGACGGGGACGCCGCGCTGGTCAGCCTGGGGCTGGATCTGCCACAGCAAACCGATAGCGTGGTGCGCGCCA
ACGATCTGATGGCGATCCGCGTCTCGCCAGGCCGGGCCGAGCTTTGGGTGCGCAGCGCAGAGGTCGACAGCATCAAGTCG
CGTCTGGCATCGCACTTGAATGAAGCCCCGCTGAACGACTGGCTACTGGGCCAGATACGTGTCGGCATCGGCCAGGTGTT
CGGCAGCACTCGCGAAGAATTCATTCCGCAGATGATCAATCTGCAAGCGGTTGGCGGCGTCAGCTTCAAGAAAGGCTGCT
ACACCGGCCAGGAAATCGTTGCCAGAATGCAATATCTTGGCAAACTCAAGCGCAGGCTTTATCGCCTGACCCTGAGCGGC
GAAGAGATCCCGCAGCCCGGTACGGCACTGTTCTCACCGGTGCATGCCAGCGCGGTGGGCAACGTTGTCATCGCCGCACA
GGATGGGCAGAACATCGAACTGCTCGCGGTATTGCAGGGTGATGCGGCAGAAGACGGCCGTATCAATCTTGGCTCGCCAG
AAGGCGCAGCGCTGCAAATGAGTGAACTGCCCTACACACTGGACAGCAAACTCGAAACTCAGCGCTAG

Upstream 100 bases:

>100_bases
CGCGCGAATCTCGCAATGGTCGCAGCAGTTTTCATGTACCCGTATGCATAACCTCGACTCGGCACTATGATGTGTCCCCG
GACTTTTTTCCCAATCTGCG

Downstream 100 bases:

>100_bases
CCAGACGAACACATTCAGCTTCCTAGTGTCTTTAGAGCGATCCAGTACAGCCTGGCGCTCTGAAGGGCGCGCGGATAACT
CTGGACACCCCCAACAGCTG

Product: glycine cleavage T protein (aminomethyl transferase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MTMAHTAFFCTLSHEGVLAVRGVDASKFLQGQLTCNLNYLNEDTSSLGARCTQKGRMQSSFRIVFEGDGCLLAMAGELIE
AQLLDLRKYAVFSKSKLTDESADWVRFGLQDGDAALVSLGLDLPQQTDSVVRANDLMAIRVSPGRAELWVRSAEVDSIKS
RLASHLNEAPLNDWLLGQIRVGIGQVFGSTREEFIPQMINLQAVGGVSFKKGCYTGQEIVARMQYLGKLKRRLYRLTLSG
EEIPQPGTALFSPVHASAVGNVVIAAQDGQNIELLAVLQGDAAEDGRINLGSPEGAALQMSELPYTLDSKLETQR

Sequences:

>Translated_315_residues
MTMAHTAFFCTLSHEGVLAVRGVDASKFLQGQLTCNLNYLNEDTSSLGARCTQKGRMQSSFRIVFEGDGCLLAMAGELIE
AQLLDLRKYAVFSKSKLTDESADWVRFGLQDGDAALVSLGLDLPQQTDSVVRANDLMAIRVSPGRAELWVRSAEVDSIKS
RLASHLNEAPLNDWLLGQIRVGIGQVFGSTREEFIPQMINLQAVGGVSFKKGCYTGQEIVARMQYLGKLKRRLYRLTLSG
EEIPQPGTALFSPVHASAVGNVVIAAQDGQNIELLAVLQGDAAEDGRINLGSPEGAALQMSELPYTLDSKLETQR
>Mature_314_residues
TMAHTAFFCTLSHEGVLAVRGVDASKFLQGQLTCNLNYLNEDTSSLGARCTQKGRMQSSFRIVFEGDGCLLAMAGELIEA
QLLDLRKYAVFSKSKLTDESADWVRFGLQDGDAALVSLGLDLPQQTDSVVRANDLMAIRVSPGRAELWVRSAEVDSIKSR
LASHLNEAPLNDWLLGQIRVGIGQVFGSTREEFIPQMINLQAVGGVSFKKGCYTGQEIVARMQYLGKLKRRLYRLTLSGE
EIPQPGTALFSPVHASAVGNVVIAAQDGQNIELLAVLQGDAAEDGRINLGSPEGAALQMSELPYTLDSKLETQR

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Escherichia coli, GI1789265, Length=247, Percent_Identity=36.8421052631579, Blast_Score=139, Evalue=3e-34,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 34238; Mature: 34106

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMAHTAFFCTLSHEGVLAVRGVDASKFLQGQLTCNLNYLNEDTSSLGARCTQKGRMQSS
CCCEEEEEEEEECCCCEEEEECCCHHHHHEEEEEEEEEECCCCHHHHHHHHHHCCCCCCC
FRIVFEGDGCLLAMAGELIEAQLLDLRKYAVFSKSKLTDESADWVRFGLQDGDAALVSLG
EEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCEEEEEEC
LDLPQQTDSVVRANDLMAIRVSPGRAELWVRSAEVDSIKSRLASHLNEAPLNDWLLGQIR
CCCCCCCCCCEEECCEEEEEECCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHH
VGIGQVFGSTREEFIPQMINLQAVGGVSFKKGCYTGQEIVARMQYLGKLKRRLYRLTLSG
HHHHHHHCCCHHHHHHHHHCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECC
EEIPQPGTALFSPVHASAVGNVVIAAQDGQNIELLAVLQGDAAEDGRINLGSPEGAALQM
CCCCCCCHHHHCCHHHHHHCCEEEEECCCCCEEEEEEEECCCCCCCEEECCCCCCCEEEC
SELPYTLDSKLETQR
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
TMAHTAFFCTLSHEGVLAVRGVDASKFLQGQLTCNLNYLNEDTSSLGARCTQKGRMQSS
CCEEEEEEEEECCCCEEEEECCCHHHHHEEEEEEEEEECCCCHHHHHHHHHHCCCCCCC
FRIVFEGDGCLLAMAGELIEAQLLDLRKYAVFSKSKLTDESADWVRFGLQDGDAALVSLG
EEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCEEEEEEC
LDLPQQTDSVVRANDLMAIRVSPGRAELWVRSAEVDSIKSRLASHLNEAPLNDWLLGQIR
CCCCCCCCCCEEECCEEEEEECCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHH
VGIGQVFGSTREEFIPQMINLQAVGGVSFKKGCYTGQEIVARMQYLGKLKRRLYRLTLSG
HHHHHHHCCCHHHHHHHHHCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECC
EEIPQPGTALFSPVHASAVGNVVIAAQDGQNIELLAVLQGDAAEDGRINLGSPEGAALQM
CCCCCCCHHHHCCHHHHHHCCEEEEECCCCCEEEEEEEECCCCCCCEEECCCCCCCEEEC
SELPYTLDSKLETQR
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]