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The map label for this gene is nadB [H]

Identifier: 66047186

GI number: 66047186

Start: 4707097

End: 4708713

Strand: Direct

Name: nadB [H]

Synonym: Psyr_3959

Alternate gene names: 66047186

Gene position: 4707097-4708713 (Clockwise)

Preceding gene: 66047174

Following gene: 66047189

Centisome position: 77.25

GC content: 60.85

Gene sequence:

>1617_bases
ATGAGTCAACATTTTCAGCATGACGTACTGGTAATCGGCAGCGGAGCTGCCGGGTTGAGCCTGGCACTGACCCTGCCCGA
CCACCTGCGGATTGCAGTCCTGAGCAAGGGCGATCTCGCCAATGGCTCGACATTCTGGGCGCAGGGCGGTGTTGCGGCAG
TGCTGGATGACACCGACACCGTGCAGTCTCATGTTGAGGACACGCTGAACGCAGGCGGCGGCCTTTGCCACGAAGACGCT
GTGCGTTTCACTGTCGAACACAGCCGCGAGGCGATTCAATGGCTGATCGATCAAGGCGTGCCTTTCACTCGTGGCGATAA
CGCAGACACCGACGAAAGTGGATTTGAATTTCACCTGACGCGTGAAGGCGGCCATAGCCATCGGCGGATCATTCATGCTG
CAGATGCCACAGGTGCCGCCATTTTTCGCACCCTGCTGGAACAGGCCATCAAGCGACCCAACATCGAATTGCTCGAGCAG
CGCGCTGCCATCGACCTGATCACCGAACGCCGTCTCGGCCTGGACGGAGAGCGCTGCCTTGGTGCCTATGTGCTCAATCG
CCACAGTGGCGAAGTGGATACGTACAGCGCGCGCTTCACCATCCTGGCGTCGGGCGGCGCCGCCAAGGTCTACCTGTACA
CCAGCAACCCTGACGGAGCCTGCGGCGACGGCATCGCCATGGCCTGGCGTTCCGGCTGCCGGGTGGCGAACCTTGAGTTC
AACCAGTTTCACCCCACTTGCCTGTATCACCCGCAAGCCAAGAGCTTTCTGATCACCGAGGCACTTCGCGGTGAAGGGGC
CTACCTCAAGCTGCCCAACGGCGAGCGCTTCATGCAACGCTTCGATGAGCGGGCAGAACTGGCCCCGCGCGATATCGTGG
CCCGCGCGATAGACCATGAGATGAAGCGACTGGGCATCGACTGTGTGTATCTGGACATCAGCCACAAGCCGGAAGCCTTC
ATCAAGACGCACTTTCCAACCGTCTATGAACGCTGCCTGGAATTTTCCATCGACATCACTCGCGAGCCGATCCCAGTGGT
CCCCGCCGCGCATTACACCTGTGGCGGCGTGATGGTGGACAGCAAGGGGCATACCGACGTGCCGGGCCTGTACGCCATCG
GCGAGACCAGCTTCACAGGCCTGCATGGCGCCAACCGCATGGCCAGCAACTCACTGCTGGAATGCTTCGTCTATGCCCGA
TCGGCCGCCGCAGACATCGAGAAACAATTGGCTGACGTGCAGATGCCCGCCGACCTTCCGGCCTGGGATGCCAGCCAGGT
CACTGACTCGGACGAGGACGTGATCATTGCGCACAACTGGGACGAGCTGAGGCGGTTCATGTGGGACTACGTCGGCATTG
TGCGCACCAACAAACGCCTGCAGCGCGCTCAGCACCGCGTGCGCTTGCTGCTGGATGAGATTGACGAGTTTTACAGCAAC
TACAAGGTCAGCCGCGACCTGATCGAGTTGCGCAATCTGGCGCAGGTGGCGGAACTGATGATCCGCTCGGCCATGCAGCG
CAAGGAAAGCCGCGGTCTGCACTACACGCTGGACTATCCCGATATGTTGCCGGAGGCGCTGGACACTATTCTGGTGCCGC
CCACCTATGCCTGCTGA

Upstream 100 bases:

>100_bases
CAATCCAGACCCGCCGTGGTCTCACATTGCCATGTAGGCGCGGCTATTGTGCCGTTACCCCAATACTTATACTAGTGGTC
GCCAAGGGCAGAATTCGCAG

Downstream 100 bases:

>100_bases
ATTTCAAACGCACGCGCAGACGTCGGTGAATGTCCGGCGTCAATGCGTCATGCGGGATACAACAACTGTTTATTGATCGT
GCCGGCCGACCGGCGATAAT

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MSQHFQHDVLVIGSGAAGLSLALTLPDHLRIAVLSKGDLANGSTFWAQGGVAAVLDDTDTVQSHVEDTLNAGGGLCHEDA
VRFTVEHSREAIQWLIDQGVPFTRGDNADTDESGFEFHLTREGGHSHRRIIHAADATGAAIFRTLLEQAIKRPNIELLEQ
RAAIDLITERRLGLDGERCLGAYVLNRHSGEVDTYSARFTILASGGAAKVYLYTSNPDGACGDGIAMAWRSGCRVANLEF
NQFHPTCLYHPQAKSFLITEALRGEGAYLKLPNGERFMQRFDERAELAPRDIVARAIDHEMKRLGIDCVYLDISHKPEAF
IKTHFPTVYERCLEFSIDITREPIPVVPAAHYTCGGVMVDSKGHTDVPGLYAIGETSFTGLHGANRMASNSLLECFVYAR
SAAADIEKQLADVQMPADLPAWDASQVTDSDEDVIIAHNWDELRRFMWDYVGIVRTNKRLQRAQHRVRLLLDEIDEFYSN
YKVSRDLIELRNLAQVAELMIRSAMQRKESRGLHYTLDYPDMLPEALDTILVPPTYAC

Sequences:

>Translated_538_residues
MSQHFQHDVLVIGSGAAGLSLALTLPDHLRIAVLSKGDLANGSTFWAQGGVAAVLDDTDTVQSHVEDTLNAGGGLCHEDA
VRFTVEHSREAIQWLIDQGVPFTRGDNADTDESGFEFHLTREGGHSHRRIIHAADATGAAIFRTLLEQAIKRPNIELLEQ
RAAIDLITERRLGLDGERCLGAYVLNRHSGEVDTYSARFTILASGGAAKVYLYTSNPDGACGDGIAMAWRSGCRVANLEF
NQFHPTCLYHPQAKSFLITEALRGEGAYLKLPNGERFMQRFDERAELAPRDIVARAIDHEMKRLGIDCVYLDISHKPEAF
IKTHFPTVYERCLEFSIDITREPIPVVPAAHYTCGGVMVDSKGHTDVPGLYAIGETSFTGLHGANRMASNSLLECFVYAR
SAAADIEKQLADVQMPADLPAWDASQVTDSDEDVIIAHNWDELRRFMWDYVGIVRTNKRLQRAQHRVRLLLDEIDEFYSN
YKVSRDLIELRNLAQVAELMIRSAMQRKESRGLHYTLDYPDMLPEALDTILVPPTYAC
>Mature_537_residues
SQHFQHDVLVIGSGAAGLSLALTLPDHLRIAVLSKGDLANGSTFWAQGGVAAVLDDTDTVQSHVEDTLNAGGGLCHEDAV
RFTVEHSREAIQWLIDQGVPFTRGDNADTDESGFEFHLTREGGHSHRRIIHAADATGAAIFRTLLEQAIKRPNIELLEQR
AAIDLITERRLGLDGERCLGAYVLNRHSGEVDTYSARFTILASGGAAKVYLYTSNPDGACGDGIAMAWRSGCRVANLEFN
QFHPTCLYHPQAKSFLITEALRGEGAYLKLPNGERFMQRFDERAELAPRDIVARAIDHEMKRLGIDCVYLDISHKPEAFI
KTHFPTVYERCLEFSIDITREPIPVVPAAHYTCGGVMVDSKGHTDVPGLYAIGETSFTGLHGANRMASNSLLECFVYARS
AAADIEKQLADVQMPADLPAWDASQVTDSDEDVIIAHNWDELRRFMWDYVGIVRTNKRLQRAQHRVRLLLDEIDEFYSNY
KVSRDLIELRNLAQVAELMIRSAMQRKESRGLHYTLDYPDMLPEALDTILVPPTYAC

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=510, Percent_Identity=32.5490196078431, Blast_Score=224, Evalue=2e-58,
Organism=Escherichia coli, GI1788928, Length=527, Percent_Identity=63.9468690702087, Blast_Score=700, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=538, Percent_Identity=32.8996282527881, Blast_Score=238, Evalue=6e-64,
Organism=Escherichia coli, GI1786942, Length=524, Percent_Identity=32.0610687022901, Blast_Score=221, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17550100, Length=532, Percent_Identity=31.9548872180451, Blast_Score=226, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17505833, Length=541, Percent_Identity=31.0536044362292, Blast_Score=224, Evalue=9e-59,
Organism=Caenorhabditis elegans, GI71986328, Length=437, Percent_Identity=24.9427917620137, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322701, Length=526, Percent_Identity=31.9391634980989, Blast_Score=226, Evalue=9e-60,
Organism=Saccharomyces cerevisiae, GI6322416, Length=534, Percent_Identity=32.0224719101124, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6320788, Length=489, Percent_Identity=25.7668711656442, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI17137288, Length=533, Percent_Identity=32.8330206378987, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24655642, Length=533, Percent_Identity=32.8330206378987, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24655647, Length=533, Percent_Identity=32.8330206378987, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24663005, Length=536, Percent_Identity=30.410447761194, Blast_Score=207, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 59894; Mature: 59763

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQHFQHDVLVIGSGAAGLSLALTLPDHLRIAVLSKGDLANGSTFWAQGGVAAVLDDTDT
CCCCCCCCEEEEECCCCCEEEEEECCCCEEEEEEECCCCCCCCEEEECCCEEEEECCHHH
VQSHVEDTLNAGGGLCHEDAVRFTVEHSREAIQWLIDQGVPFTRGDNADTDESGFEFHLT
HHHHHHHHHHCCCCCCCHHHHEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEE
REGGHSHRRIIHAADATGAAIFRTLLEQAIKRPNIELLEQRAAIDLITERRLGLDGERCL
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
GAYVLNRHSGEVDTYSARFTILASGGAAKVYLYTSNPDGACGDGIAMAWRSGCRVANLEF
HHHHHHCCCCCCEEEEEEEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHCCCEEEEEEC
NQFHPTCLYHPQAKSFLITEALRGEGAYLKLPNGERFMQRFDERAELAPRDIVARAIDHE
CCCCCEEEECCCCCHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
MKRLGIDCVYLDISHKPEAFIKTHFPTVYERCLEFSIDITREPIPVVPAAHYTCGGVMVD
HHHCCCEEEEEEECCCCCHHHHHCCHHHHHHHHHHCEECCCCCCCCCCCCCEECCCEEEC
SKGHTDVPGLYAIGETSFTGLHGANRMASNSLLECFVYARSAAADIEKQLADVQMPADLP
CCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AWDASQVTDSDEDVIIAHNWDELRRFMWDYVGIVRTNKRLQRAQHRVRLLLDEIDEFYSN
CCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YKVSRDLIELRNLAQVAELMIRSAMQRKESRGLHYTLDYPDMLPEALDTILVPPTYAC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure 
SQHFQHDVLVIGSGAAGLSLALTLPDHLRIAVLSKGDLANGSTFWAQGGVAAVLDDTDT
CCCCCCCEEEEECCCCCEEEEEECCCCEEEEEEECCCCCCCCEEEECCCEEEEECCHHH
VQSHVEDTLNAGGGLCHEDAVRFTVEHSREAIQWLIDQGVPFTRGDNADTDESGFEFHLT
HHHHHHHHHHCCCCCCCHHHHEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEE
REGGHSHRRIIHAADATGAAIFRTLLEQAIKRPNIELLEQRAAIDLITERRLGLDGERCL
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
GAYVLNRHSGEVDTYSARFTILASGGAAKVYLYTSNPDGACGDGIAMAWRSGCRVANLEF
HHHHHHCCCCCCEEEEEEEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHCCCEEEEEEC
NQFHPTCLYHPQAKSFLITEALRGEGAYLKLPNGERFMQRFDERAELAPRDIVARAIDHE
CCCCCEEEECCCCCHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
MKRLGIDCVYLDISHKPEAFIKTHFPTVYERCLEFSIDITREPIPVVPAAHYTCGGVMVD
HHHCCCEEEEEEECCCCCHHHHHCCHHHHHHHHHHCEECCCCCCCCCCCCCEECCCEEEC
SKGHTDVPGLYAIGETSFTGLHGANRMASNSLLECFVYARSAAADIEKQLADVQMPADLP
CCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AWDASQVTDSDEDVIIAHNWDELRRFMWDYVGIVRTNKRLQRAQHRVRLLLDEIDEFYSN
CCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YKVSRDLIELRNLAQVAELMIRSAMQRKESRGLHYTLDYPDMLPEALDTILVPPTYAC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7737518; 10984043; 7961421 [H]