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The map label for this gene is cobS [H]

Identifier: 66046901

GI number: 66046901

Start: 4359106

End: 4359837

Strand: Reverse

Name: cobS [H]

Synonym: Psyr_3672

Alternate gene names: 66046901

Gene position: 4359837-4359106 (Counterclockwise)

Preceding gene: 66046902

Following gene: 66046900

Centisome position: 71.55

GC content: 65.98

Gene sequence:

>732_bases
ATGCTGCCGTTCTGGATCGCCCTGCAATTTCTCGGCAGCCTGCCGATCAGGCTGCCCGGCATGCCGAGCCCCGAGGAGCT
GGGGCGTTCCTTGCTGTTTTACCCGTTGGTGGGGGCGGTGTTCGGCACGCTGCTGCTGGGCTTCAACACGCTGCTCAGTG
GTGCGCCGCTGATGCTGCACGCCGCGCTGGTACTCACTGCCTGGGTGTTGCTCAGCGGCGGCCTGCATCTGGACGGTCTG
GCCGACAGTGCTGACGCCTGGCTGGGCGGCTTCGGTGACCGTGAACGCACCTTGAAGATCATGAAAGACCCACGCAGCGG
GCCTATCGCCGTGGTCACGCTGGTCCTGGTGCTGCTGCTCAAGTTTGCTGCGATTCTGGCGCTGATCGAGAGCCATGCCA
GCGTCTGGCTGCTGTTGGCACCGGTGATCGGGCGCGCGGCCATGCTTGGGCTGTTTCTGGGGACGCCGTATGTGCGTTCC
GGCGGGCTGGGGCAGGCGTTGGCCGATCACCTGCCGCGCGGCCCTGGACGCAAGGTTTTGCTCGCCACCGCCATTGCGTG
CGTGCTGCTCGCCGGCTGGTCGGGGGTAGCCGTATTGCTGGTCTGCGCCGTCTGTTTCTTCTGGCTGCGGCAACTGATGA
TGCGCCGTCTGGGCGGATGCACCGGTGATACAGCCGGGGCATTGCTCGAGCTGCTGGAGTTGGCGGTGCTGCTGACGCTG
GCGCTGCTTTGA

Upstream 100 bases:

>100_bases
CGTGGCCTGCCGCGTGAGCAACTGTTGCAGGTGGTGGTCGGGCATGGCGCGCTGTTGTCGATTCAGGTCGCTGCTGATCG
CGTACTCACGGAGGTCTGAG

Downstream 100 bases:

>100_bases
GACCCTGCGCAACGCTTTGTTGACAGCTTTTACCGGCAGCGCGTAGCTTCGCCAATCGCGGGTAAAACCATGTAACACAC
AGCGCAGGGAGCTCAACCAT

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MLPFWIALQFLGSLPIRLPGMPSPEELGRSLLFYPLVGAVFGTLLLGFNTLLSGAPLMLHAALVLTAWVLLSGGLHLDGL
ADSADAWLGGFGDRERTLKIMKDPRSGPIAVVTLVLVLLLKFAAILALIESHASVWLLLAPVIGRAAMLGLFLGTPYVRS
GGLGQALADHLPRGPGRKVLLATAIACVLLAGWSGVAVLLVCAVCFFWLRQLMMRRLGGCTGDTAGALLELLELAVLLTL
ALL

Sequences:

>Translated_243_residues
MLPFWIALQFLGSLPIRLPGMPSPEELGRSLLFYPLVGAVFGTLLLGFNTLLSGAPLMLHAALVLTAWVLLSGGLHLDGL
ADSADAWLGGFGDRERTLKIMKDPRSGPIAVVTLVLVLLLKFAAILALIESHASVWLLLAPVIGRAAMLGLFLGTPYVRS
GGLGQALADHLPRGPGRKVLLATAIACVLLAGWSGVAVLLVCAVCFFWLRQLMMRRLGGCTGDTAGALLELLELAVLLTL
ALL
>Mature_243_residues
MLPFWIALQFLGSLPIRLPGMPSPEELGRSLLFYPLVGAVFGTLLLGFNTLLSGAPLMLHAALVLTAWVLLSGGLHLDGL
ADSADAWLGGFGDRERTLKIMKDPRSGPIAVVTLVLVLLLKFAAILALIESHASVWLLLAPVIGRAAMLGLFLGTPYVRS
GGLGQALADHLPRGPGRKVLLATAIACVLLAGWSGVAVLLVCAVCFFWLRQLMMRRLGGCTGDTAGALLELLELAVLLTL
ALL

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=249, Percent_Identity=37.7510040160643, Blast_Score=101, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 25648; Mature: 25648

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPFWIALQFLGSLPIRLPGMPSPEELGRSLLFYPLVGAVFGTLLLGFNTLLSGAPLMLH
CCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
AALVLTAWVLLSGGLHLDGLADSADAWLGGFGDRERTLKIMKDPRSGPIAVVTLVLVLLL
HHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
KFAAILALIESHASVWLLLAPVIGRAAMLGLFLGTPYVRSGGLGQALADHLPRGPGRKVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHCCCCCCCHHH
LATAIACVLLAGWSGVAVLLVCAVCFFWLRQLMMRRLGGCTGDTAGALLELLELAVLLTL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
ALL
HHC
>Mature Secondary Structure
MLPFWIALQFLGSLPIRLPGMPSPEELGRSLLFYPLVGAVFGTLLLGFNTLLSGAPLMLH
CCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
AALVLTAWVLLSGGLHLDGLADSADAWLGGFGDRERTLKIMKDPRSGPIAVVTLVLVLLL
HHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
KFAAILALIESHASVWLLLAPVIGRAAMLGLFLGTPYVRSGGLGQALADHLPRGPGRKVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHCCCCCCCHHH
LATAIACVLLAGWSGVAVLLVCAVCFFWLRQLMMRRLGGCTGDTAGALLELLELAVLLTL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
ALL
HHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA