Definition Pseudomonas syringae pv. syringae B728a, complete genome.
Accession NC_007005
Length 6,093,698

Click here to switch to the map view.

The map label for this gene is ygfF [C]

Identifier: 66046774

GI number: 66046774

Start: 4222768

End: 4223511

Strand: Reverse

Name: ygfF [C]

Synonym: Psyr_3545

Alternate gene names: 66046774

Gene position: 4223511-4222768 (Counterclockwise)

Preceding gene: 66046776

Following gene: 66046773

Centisome position: 69.31

GC content: 61.83

Gene sequence:

>744_bases
ATGGGCGTCATCGTAATCACTGGCGGCAGCCGTGGCATCGGCGCAAGTGCAGCCGAACACGTGGCTCGACGCGGGATGGG
GGTCATCCTTACCTACAACGCCAACCCGGAGGCCGCCGCAACGGTGGTCGAGCGTATCGCGCAGGCCGGTGGCAAGGCAG
TTGCGCTCAGGCTGGATGTCGCTGACGTCGGCAGCTTCGAGGCGTTTCGAGCGTCAGTGTGCTCGGTCCTGCAAGAAGTC
TGGGGCGTCGCAACACTGAGCGGTCTGGTCAATAACGCGGGTTACGGGCTGTTCAATCCGTTGGAGACGGTCAGCGAAGC
GCAGTTCGATGGCCTGTTCAATGTGCATCTCAAAGGCCCTTTTTTCCTCACCCAGACATTGCTGCCGTTGATGGCGGAAA
ACGCCAGCATCGTCAATCTGACCAGTGCCACCACTCGCGTCGCCACTGCGGGGGTCGCTCCCTATGCGGCGTTCAAGGGC
GGGCTTGAAGTCTTGACCCGCTATATGGCTGTCGAGTTTGGCGAGCGGCGCATAAGGGCCAACGCCGTATCACCCGGCGC
CATTCGTACCGAACTGGGCGGTGGGCTCAATGATGAGTTCGAGGCGATGCTGGCAGCACAGACGGCCTTGGGCAGGGTCG
GCGAGCCGCAAGATGTGGCACGCGTCATCGCCATGCTGCTGTCCGAAGAGGCTGGCTGGATCAATGCGCAGACTATCGAA
GTCGCAGGCGGCTACAACATCTGA

Upstream 100 bases:

>100_bases
CGAAAAGCAGAAACAGGCAGGTTTCGAGCAGGAACGGGTACGCTCCCGGTGATGGCGCTGGGTACTGTGGAGACACATTC
AAACCACAGAGGAACATCAC

Downstream 100 bases:

>100_bases
GGGCGACCCATGCTCGATCATAGCGTTCTACGCGCAACAGCCGTTCTGTACCCCGATGGCTACAGCCCCGACTTCGTCTC
GAAAAAAAGGCAGCACGTCC

Product: Short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: ORFC [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MGVIVITGGSRGIGASAAEHVARRGMGVILTYNANPEAAATVVERIAQAGGKAVALRLDVADVGSFEAFRASVCSVLQEV
WGVATLSGLVNNAGYGLFNPLETVSEAQFDGLFNVHLKGPFFLTQTLLPLMAENASIVNLTSATTRVATAGVAPYAAFKG
GLEVLTRYMAVEFGERRIRANAVSPGAIRTELGGGLNDEFEAMLAAQTALGRVGEPQDVARVIAMLLSEEAGWINAQTIE
VAGGYNI

Sequences:

>Translated_247_residues
MGVIVITGGSRGIGASAAEHVARRGMGVILTYNANPEAAATVVERIAQAGGKAVALRLDVADVGSFEAFRASVCSVLQEV
WGVATLSGLVNNAGYGLFNPLETVSEAQFDGLFNVHLKGPFFLTQTLLPLMAENASIVNLTSATTRVATAGVAPYAAFKG
GLEVLTRYMAVEFGERRIRANAVSPGAIRTELGGGLNDEFEAMLAAQTALGRVGEPQDVARVIAMLLSEEAGWINAQTIE
VAGGYNI
>Mature_246_residues
GVIVITGGSRGIGASAAEHVARRGMGVILTYNANPEAAATVVERIAQAGGKAVALRLDVADVGSFEAFRASVCSVLQEVW
GVATLSGLVNNAGYGLFNPLETVSEAQFDGLFNVHLKGPFFLTQTLLPLMAENASIVNLTSATTRVATAGVAPYAAFKGG
LEVLTRYMAVEFGERRIRANAVSPGAIRTELGGGLNDEFEAMLAAQTALGRVGEPQDVARVIAMLLSEEAGWINAQTIEV
AGGYNI

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=247, Percent_Identity=27.9352226720648, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI15277342, Length=251, Percent_Identity=30.6772908366534, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI5031737, Length=251, Percent_Identity=32.2709163346614, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI7705925, Length=247, Percent_Identity=28.7449392712551, Blast_Score=87, Evalue=9e-18,
Organism=Homo sapiens, GI126723750, Length=248, Percent_Identity=25.8064516129032, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4503301, Length=233, Percent_Identity=26.6094420600858, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI40254992, Length=253, Percent_Identity=30.0395256916996, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI19923817, Length=267, Percent_Identity=25.0936329588015, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI59889578, Length=170, Percent_Identity=32.3529411764706, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI33667109, Length=209, Percent_Identity=32.5358851674641, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI2367175, Length=257, Percent_Identity=32.6848249027237, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1787335, Length=244, Percent_Identity=33.6065573770492, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787905, Length=243, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=7e-24,
Organism=Escherichia coli, GI1789378, Length=246, Percent_Identity=30.8943089430894, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1790717, Length=245, Percent_Identity=28.5714285714286, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI2367365, Length=243, Percent_Identity=32.0987654320988, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788459, Length=246, Percent_Identity=26.8292682926829, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786701, Length=198, Percent_Identity=32.3232323232323, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI87082100, Length=258, Percent_Identity=28.6821705426357, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789208, Length=248, Percent_Identity=26.2096774193548, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI87082160, Length=245, Percent_Identity=24.8979591836735, Blast_Score=68, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25147288, Length=249, Percent_Identity=28.5140562248996, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17561402, Length=250, Percent_Identity=29.2, Blast_Score=88, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17562910, Length=263, Percent_Identity=30.7984790874525, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17536651, Length=250, Percent_Identity=26.8, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17560676, Length=253, Percent_Identity=27.2727272727273, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17536025, Length=251, Percent_Identity=27.8884462151394, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71994600, Length=254, Percent_Identity=28.3464566929134, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71994604, Length=254, Percent_Identity=28.740157480315, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI115534694, Length=252, Percent_Identity=26.984126984127, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17555706, Length=251, Percent_Identity=28.2868525896414, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17559104, Length=261, Percent_Identity=29.5019157088123, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17561272, Length=214, Percent_Identity=28.5046728971963, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17562990, Length=259, Percent_Identity=28.957528957529, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17560150, Length=260, Percent_Identity=27.6923076923077, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17508651, Length=254, Percent_Identity=27.1653543307087, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71982365, Length=194, Percent_Identity=29.3814432989691, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17562908, Length=258, Percent_Identity=29.0697674418605, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17560332, Length=262, Percent_Identity=29.3893129770992, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17544670, Length=265, Percent_Identity=26.4150943396226, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17531453, Length=266, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=195, Percent_Identity=30.2564102564103, Blast_Score=63, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24643142, Length=248, Percent_Identity=33.0645161290323, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24639444, Length=246, Percent_Identity=33.3333333333333, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI28571526, Length=257, Percent_Identity=33.852140077821, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21357041, Length=251, Percent_Identity=33.8645418326693, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24644339, Length=251, Percent_Identity=32.2709163346614, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI23397609, Length=253, Percent_Identity=29.2490118577075, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21355319, Length=252, Percent_Identity=28.968253968254, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24644337, Length=169, Percent_Identity=34.9112426035503, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 25699; Mature: 25567

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MGVIVITGGSRGIGASAAEHVARRGMGVILTYNANPEAAATVVERIAQAGGKAVALRLDV
CEEEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEH
ADVGSFEAFRASVCSVLQEVWGVATLSGLVNNAGYGLFNPLETVSEAQFDGLFNVHLKGP
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCEEEEEECCC
FFLTQTLLPLMAENASIVNLTSATTRVATAGVAPYAAFKGGLEVLTRYMAVEFGERRIRA
HHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
NAVSPGAIRTELGGGLNDEFEAMLAAQTALGRVGEPQDVARVIAMLLSEEAGWINAQTIE
CCCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEE
VAGGYNI
ECCCCCC
>Mature Secondary Structure 
GVIVITGGSRGIGASAAEHVARRGMGVILTYNANPEAAATVVERIAQAGGKAVALRLDV
EEEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEH
ADVGSFEAFRASVCSVLQEVWGVATLSGLVNNAGYGLFNPLETVSEAQFDGLFNVHLKGP
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCEEEEEECCC
FFLTQTLLPLMAENASIVNLTSATTRVATAGVAPYAAFKGGLEVLTRYMAVEFGERRIRA
HHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
NAVSPGAIRTELGGGLNDEFEAMLAAQTALGRVGEPQDVARVIAMLLSEEAGWINAQTIE
CCCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEE
VAGGYNI
ECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8868443 [H]