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The map label for this gene is hipO [H]

Identifier: 66046752

GI number: 66046752

Start: 4199121

End: 4200278

Strand: Reverse

Name: hipO [H]

Synonym: Psyr_3523

Alternate gene names: 66046752

Gene position: 4200278-4199121 (Counterclockwise)

Preceding gene: 66046753

Following gene: 66046750

Centisome position: 68.93

GC content: 63.21

Gene sequence:

>1158_bases
ATGTCCATTTCAAAGACGTTGATTGACGAAGCAACCGTGTGGCGCCGCACCCTCCATGCCGCGCCGGAACTGGGGTTTCA
GGAGCTGAAAACCTCCGACAAGGTGGCTGAGTTGCTGAGCGGTTTCGGCATCGAGATTCACCGTGGGCTGGGCGGCACTG
GCGTGGTTGGCACCCTGCGTAACGGCGACGGTCCGACCATCGGCCTGCGTGCCGACATGGATGCCTTGCCGATTCAGGAG
CTGGGCGACAGCGTTCACAAGTCGACCCACAAGGGCTGCATGCACGCCTGCGGCCATGATGGCCACACCGCGATCCTGCT
GGCCACCGCGAGGCATCTGGCCGAGACTCGGCGCTTTCGCGGCACGGTGCATTTCGTCTTCCAGCCTGCCGAAGAGAACC
TCGGCGGCGCACAGCGAATGATCGAAGACGGACTGTTCGAACGCTTTCCGATGGAAGCCATCTACGGCCTGCACAACTGG
CCGGGTGTGCCTGCGGGCAAGGTGGTGATCAATCCGGGGCCGATGATGGCGTCGCTCGACACCTTTGAAATCACCCTGAC
CGGCAAAGGCAGCCATGCGGCCATGCCGGACAAGGGCAATGATCCGATCGTGGCCGCGGCCGAGCTGGTGCTGGGGCTGC
AAACCATTGTCTCCCGGCGCCTGTCGCCGCTCGATTCGGCGGTGGTCAGCATCACTCAGTTCAATGCCGGCGAGGCGATC
AACGTGATCCCGGAAACCGCCACGTTGCGTGGCACCGTGCGCTGCCTGCAAACCCCTGTGCGCGAAAAGGTTCAGCAACT
GATCGGCGAGTTCGTCGAGCGTTTGCCGACGGCCTTCGGCGTGCGCGGCGAGCTGGTTTACAACGTCGGCTACCCGGTGA
CCGAAAACCACGTCGAAGCGGCTGCCACGATCCGTCGTGCAGCAGTCGCCGCAGTCGGCGAGGCCAACGTGCAGTGGGGC
TGCAACCCGTCGATGGCCTCTGAAGACTTCGCCTTCATGCTGCATGCATGTCCCGGTGCTTACATCTGGATGGGCGTCGA
CGGCGAGAAGCCGTCAGCGGCGTTGCACAACCCTTACTACGACTTCAACGACCAGGTGATCGAACCCGGCGTTGCGGTCT
GGACCTCGCTGGTGGAGCAGTGTTTGCCGGTGGCTTGA

Upstream 100 bases:

>100_bases
GATGCCCTGCGCGAGTACAGTGAAGTGAAAAGCGTCTGGGTCGAAACCGCCGCCAAGCCAGCCGCCAGCTTTGGCTACGG
CGCGAGTCTGGAGTAAGCAC

Downstream 100 bases:

>100_bases
ATGGCTCTCGCGCTTACGGCTGCCTTTGATGCTGGCCGTAGGTCATAGGAGCGAAGCGGTTCGCGAAAAGGACGGTACCT
CCGCAGGGGATGTACCGTCC

Product: peptidase M20D, amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MSISKTLIDEATVWRRTLHAAPELGFQELKTSDKVAELLSGFGIEIHRGLGGTGVVGTLRNGDGPTIGLRADMDALPIQE
LGDSVHKSTHKGCMHACGHDGHTAILLATARHLAETRRFRGTVHFVFQPAEENLGGAQRMIEDGLFERFPMEAIYGLHNW
PGVPAGKVVINPGPMMASLDTFEITLTGKGSHAAMPDKGNDPIVAAAELVLGLQTIVSRRLSPLDSAVVSITQFNAGEAI
NVIPETATLRGTVRCLQTPVREKVQQLIGEFVERLPTAFGVRGELVYNVGYPVTENHVEAAATIRRAAVAAVGEANVQWG
CNPSMASEDFAFMLHACPGAYIWMGVDGEKPSAALHNPYYDFNDQVIEPGVAVWTSLVEQCLPVA

Sequences:

>Translated_385_residues
MSISKTLIDEATVWRRTLHAAPELGFQELKTSDKVAELLSGFGIEIHRGLGGTGVVGTLRNGDGPTIGLRADMDALPIQE
LGDSVHKSTHKGCMHACGHDGHTAILLATARHLAETRRFRGTVHFVFQPAEENLGGAQRMIEDGLFERFPMEAIYGLHNW
PGVPAGKVVINPGPMMASLDTFEITLTGKGSHAAMPDKGNDPIVAAAELVLGLQTIVSRRLSPLDSAVVSITQFNAGEAI
NVIPETATLRGTVRCLQTPVREKVQQLIGEFVERLPTAFGVRGELVYNVGYPVTENHVEAAATIRRAAVAAVGEANVQWG
CNPSMASEDFAFMLHACPGAYIWMGVDGEKPSAALHNPYYDFNDQVIEPGVAVWTSLVEQCLPVA
>Mature_384_residues
SISKTLIDEATVWRRTLHAAPELGFQELKTSDKVAELLSGFGIEIHRGLGGTGVVGTLRNGDGPTIGLRADMDALPIQEL
GDSVHKSTHKGCMHACGHDGHTAILLATARHLAETRRFRGTVHFVFQPAEENLGGAQRMIEDGLFERFPMEAIYGLHNWP
GVPAGKVVINPGPMMASLDTFEITLTGKGSHAAMPDKGNDPIVAAAELVLGLQTIVSRRLSPLDSAVVSITQFNAGEAIN
VIPETATLRGTVRCLQTPVREKVQQLIGEFVERLPTAFGVRGELVYNVGYPVTENHVEAAATIRRAAVAAVGEANVQWGC
NPSMASEDFAFMLHACPGAYIWMGVDGEKPSAALHNPYYDFNDQVIEPGVAVWTSLVEQCLPVA

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=417, Percent_Identity=28.0575539568345, Blast_Score=111, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41361; Mature: 41230

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISKTLIDEATVWRRTLHAAPELGFQELKTSDKVAELLSGFGIEIHRGLGGTGVVGTLR
CCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEE
NGDGPTIGLRADMDALPIQELGDSVHKSTHKGCMHACGHDGHTAILLATARHLAETRRFR
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHC
GTVHFVFQPAEENLGGAQRMIEDGLFERFPMEAIYGLHNWPGVPAGKVVINPGPMMASLD
CEEEEEEECCHHCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCEEEECCCCCEEECC
TFEITLTGKGSHAAMPDKGNDPIVAAAELVLGLQTIVSRRLSPLDSAVVSITQFNAGEAI
EEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEECCCCCEE
NVIPETATLRGTVRCLQTPVREKVQQLIGEFVERLPTAFGVRGELVYNVGYPVTENHVEA
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHH
AATIRRAAVAAVGEANVQWGCNPSMASEDFAFMLHACPGAYIWMGVDGEKPSAALHNPYY
HHHHHHHHHHHHCCCCCEECCCCCCCCCHHHHHEECCCCEEEEEECCCCCCCHHHCCCCC
DFNDQVIEPGVAVWTSLVEQCLPVA
CCCCCHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SISKTLIDEATVWRRTLHAAPELGFQELKTSDKVAELLSGFGIEIHRGLGGTGVVGTLR
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEE
NGDGPTIGLRADMDALPIQELGDSVHKSTHKGCMHACGHDGHTAILLATARHLAETRRFR
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHC
GTVHFVFQPAEENLGGAQRMIEDGLFERFPMEAIYGLHNWPGVPAGKVVINPGPMMASLD
CEEEEEEECCHHCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCEEEECCCCCEEECC
TFEITLTGKGSHAAMPDKGNDPIVAAAELVLGLQTIVSRRLSPLDSAVVSITQFNAGEAI
EEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEECCCCCEE
NVIPETATLRGTVRCLQTPVREKVQQLIGEFVERLPTAFGVRGELVYNVGYPVTENHVEA
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHH
AATIRRAAVAAVGEANVQWGCNPSMASEDFAFMLHACPGAYIWMGVDGEKPSAALHNPYY
HHHHHHHHHHHHCCCCCEECCCCCCCCCHHHHHEECCCCEEEEEECCCCCCCHHHCCCCC
DFNDQVIEPGVAVWTSLVEQCLPVA
CCCCCHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]