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The map label for this gene is gcvA [H]

Identifier: 66046750

GI number: 66046750

Start: 4196563

End: 4197474

Strand: Reverse

Name: gcvA [H]

Synonym: Psyr_3521

Alternate gene names: 66046750

Gene position: 4197474-4196563 (Counterclockwise)

Preceding gene: 66046752

Following gene: 66046746

Centisome position: 68.88

GC content: 62.5

Gene sequence:

>912_bases
ATGAGCAGAATCTTCAACGCACAGATGCACGCCTGGCTACAGGTGTTCGCCTGCGCGGCCCGTCACCTGTCCTTCACCCG
CTGCGCGGAAGAATTGCACGTCACCCCCGGCGCGATCAGCCAGCAGATGCGCCAGCTCGAAGAGCGCCTGGGCTTTGCGC
TGTTTCACCGGGTCGGGCGCGGGCTTGAGCTGACCGCTGAAGGTCAGCGTCTGGCGGTGGTCGCCAACGAAGTGCAGAGC
CGTATCAGCGAAGAACTGCGCCTGCTGTATTCCGGGCGTATCGGCGGGGTGTTCAAGCTGCGCTGCATTCCATCATTCCT
GAGCAAATGGCTGATGCCGCGCCTGCCACGCCTGCAGGCGGCGTTTCCGGACATCCAGTTGCGGATCATTGCCGAGGACA
GCAGCGGCTCGTTGCGTGATGACGATTTCGATCTGGCCATCGACCTGAACGACGGCAGCTATCCGGGGTTGTCGACTACG
CCCTTGCTGGAGGAGGAGCTGTTCGCGGTCTGCTCGCCAGACCTGCTGGTGGGCAAGCCGCCGCTGGATACGCCCAGTCA
GCTGGTGCACTTTCCGTTGTTGCACGACATCACTGCCTGGCGCGGCAGCTATGAGTATGCGGAGTGGGAGTTCTACCTGA
CGGCCATCGGTGCCGATGGCATCGACGTGCGTCGCGGCCACACGTTTAACCGTAATCACCTGACCATCGACGCCGCGCGT
ATGGGCATGGGTGTGGCTATTGCGCGCAAGGCGCTGATCACCGATGAGCTGGAGCAGGGCTTGTTGATCGTGCCGTTCGG
TCATCCGATCAAGGCCAGAAAGAAATACGTGCTGGCCTATCGTGAAGGGGCGCTCAGCACCCCGTCACGTCGCGCAGTGC
ATGACTGGCTGGTGAACGAGGCGCAGGGCTGA

Upstream 100 bases:

>100_bases
CGGGAATTTGGTATTCCCCTTTTTCTGTTACTCATCTTTGGCTTCGGCTGTCGAAACGACAAACGATTGTTGCTATGCAA
CGCTTTAGCGGAACTCTTCA

Downstream 100 bases:

>100_bases
CTCGCGCCGAATCAGGCGGTGCGGATCAATGGAACAGCTCGGCGATCAATACCGCGCCGATGAACGTACCGAAGTTGGCC
AGGAACGACACCAGCACAAT

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MSRIFNAQMHAWLQVFACAARHLSFTRCAEELHVTPGAISQQMRQLEERLGFALFHRVGRGLELTAEGQRLAVVANEVQS
RISEELRLLYSGRIGGVFKLRCIPSFLSKWLMPRLPRLQAAFPDIQLRIIAEDSSGSLRDDDFDLAIDLNDGSYPGLSTT
PLLEEELFAVCSPDLLVGKPPLDTPSQLVHFPLLHDITAWRGSYEYAEWEFYLTAIGADGIDVRRGHTFNRNHLTIDAAR
MGMGVAIARKALITDELEQGLLIVPFGHPIKARKKYVLAYREGALSTPSRRAVHDWLVNEAQG

Sequences:

>Translated_303_residues
MSRIFNAQMHAWLQVFACAARHLSFTRCAEELHVTPGAISQQMRQLEERLGFALFHRVGRGLELTAEGQRLAVVANEVQS
RISEELRLLYSGRIGGVFKLRCIPSFLSKWLMPRLPRLQAAFPDIQLRIIAEDSSGSLRDDDFDLAIDLNDGSYPGLSTT
PLLEEELFAVCSPDLLVGKPPLDTPSQLVHFPLLHDITAWRGSYEYAEWEFYLTAIGADGIDVRRGHTFNRNHLTIDAAR
MGMGVAIARKALITDELEQGLLIVPFGHPIKARKKYVLAYREGALSTPSRRAVHDWLVNEAQG
>Mature_302_residues
SRIFNAQMHAWLQVFACAARHLSFTRCAEELHVTPGAISQQMRQLEERLGFALFHRVGRGLELTAEGQRLAVVANEVQSR
ISEELRLLYSGRIGGVFKLRCIPSFLSKWLMPRLPRLQAAFPDIQLRIIAEDSSGSLRDDDFDLAIDLNDGSYPGLSTTP
LLEEELFAVCSPDLLVGKPPLDTPSQLVHFPLLHDITAWRGSYEYAEWEFYLTAIGADGIDVRRGHTFNRNHLTIDAARM
GMGVAIARKALITDELEQGLLIVPFGHPIKARKKYVLAYREGALSTPSRRAVHDWLVNEAQG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=289, Percent_Identity=37.3702422145329, Blast_Score=190, Evalue=9e-50,
Organism=Escherichia coli, GI1788706, Length=283, Percent_Identity=31.8021201413428, Blast_Score=147, Evalue=8e-37,
Organism=Escherichia coli, GI1786448, Length=298, Percent_Identity=29.5302013422819, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI157672245, Length=208, Percent_Identity=33.6538461538462, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI145693105, Length=187, Percent_Identity=25.1336898395722, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786401, Length=299, Percent_Identity=25.0836120401338, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693193, Length=172, Percent_Identity=29.0697674418605, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87081978, Length=253, Percent_Identity=25.296442687747, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790262, Length=175, Percent_Identity=28, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33988; Mature: 33857

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS50931 HTH_LYSR ; PS00290 IG_MHC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIFNAQMHAWLQVFACAARHLSFTRCAEELHVTPGAISQQMRQLEERLGFALFHRVGR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
GLELTAEGQRLAVVANEVQSRISEELRLLYSGRIGGVFKLRCIPSFLSKWLMPRLPRLQA
CEEEEECCCEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCCCCHHH
AFPDIQLRIIAEDSSGSLRDDDFDLAIDLNDGSYPGLSTTPLLEEELFAVCSPDLLVGKP
CCCCEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCEEECCC
PLDTPSQLVHFPLLHDITAWRGSYEYAEWEFYLTAIGADGIDVRRGHTFNRNHLTIDAAR
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCEECCCCCCCCCEEEEEHHH
MGMGVAIARKALITDELEQGLLIVPFGHPIKARKKYVLAYREGALSTPSRRAVHDWLVNE
HCCCHHHHHHHHHHHHHHCCEEEEECCCCHHHCCEEEEEEECCCCCCCCHHHHHHHHHHC
AQG
CCC
>Mature Secondary Structure 
SRIFNAQMHAWLQVFACAARHLSFTRCAEELHVTPGAISQQMRQLEERLGFALFHRVGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
GLELTAEGQRLAVVANEVQSRISEELRLLYSGRIGGVFKLRCIPSFLSKWLMPRLPRLQA
CEEEEECCCEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCCCCHHH
AFPDIQLRIIAEDSSGSLRDDDFDLAIDLNDGSYPGLSTTPLLEEELFAVCSPDLLVGKP
CCCCEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCEEECCC
PLDTPSQLVHFPLLHDITAWRGSYEYAEWEFYLTAIGADGIDVRRGHTFNRNHLTIDAAR
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCEECCCCCCCCCEEEEEHHH
MGMGVAIARKALITDELEQGLLIVPFGHPIKARKKYVLAYREGALSTPSRRAVHDWLVNE
HCCCHHHHHHHHHHHHHHCCEEEEECCCCHHHCCEEEEEEECCCCCCCCHHHHHHHHHHC
AQG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]